30 research outputs found
Effect of Yakae-Prajamduen-Jamod Traditional Thai Remedy on Cognitive Impairment in an Ovariectomized Mouse Model and Its Mechanism of Action
No full textCognitive impairment is a neurological symptom caused by reduced estrogen levels in menopausal women. The Thai traditional medicine, Yakae-Prajamduen-Jamod (YPJ), is a formula consisting of 23 medicinal herbs and has long been used to treat menopausal symptoms in Thailand. In the present study, we investigated the effects of YPJ on cognitive deficits and its underlying mechanisms of action in ovariectomized (OVX) mice, an animal model of menopause. OVX mice showed cognitive deficits in the Y-maze, the novel object recognition test, and the Morris water maze. The serum corticosterone (CORT) level was significantly increased in OVX mice. Superoxide dismutase and catalase activities were reduced, while the mRNA expression of IL-1β, IL-6, and TNF-α inflammatory cytokines were up-regulated in the frontal cortex and hippocampus of OVX mice. These alterations were attenuated by daily treatment with either YPJ or 17β-estradiol. HPLC analysis revealed that YPJ contained antioxidant and phytoestrogen constituents including gallic acid, myricetin, quercetin, luteolin, genistein, and coumestrol. These results suggest that YPJ exerts its ameliorative effects on OVX-induced cognitive deficits in part by mitigating HPA axis overactivation, neuroinflammation, and oxidative brain damage. Therefore, YPJ may be a novel alternative therapeutic medicine suitable for the treatment of cognitive deficits during the menopausal transition
Application of <i>Dipterocarpus alatus</i> oil for Permeation Enhancement of Some Compounds
No full textPotential of Alternanthera philoxeroides on Improvement of Anxiety-Like Behavior Induced By Ovariectomized Mice Model
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Performance in Daily Living Activities of the Elderly While Living at Home or Being Home-bound in a Thai Suburban Community
No full textAbstractThe purpose of this study was to examine the ability of elderly people in performing their daily activities while living at home or being home-bound in the community. Data collection included interviews and observation of their performance at their home in the community. The study found that all of the elderly communicated independently. They also acted independently in interpersonal interactions and relationships, but they needed assistance from people or equipment in some activities such as walking and bodily care. Most of the home-bound elderly performed activities of daily living independently, whereas the elderly living at home were dependent when using transportation and driving
Optimized Extraction Method for Kleeb Bua Daeng Formula with the Aid of the Experimental Design
No full textKleeb Bua Daeng formula is one of the popular remedies sold in Chao Phya Abhaibhubejhr Hospital, Thailand. This formula contains Piper nigrum L., Nelumbo nucifera Gaertn., and Centella asiatica L. as active components. Owing to getting the highest content of its phytochemical compounds, the conditions of solvent extraction for this formula were optimized. The type of solvent, number of extraction times, and ratio between the material and solvent were varied in this study using the Box–Behnken design. The important phytochemical constituents (total phenolics, flavonoids, carotenoids, and anthocyanins) were also determined. From the result of this study, it was found that the highest content of each total active compound was obtained from different conditions such as the optimal extraction condition of phenolic content was obtained using methanol as solvent, one time of extraction, and the ratio of powder and solvent was 1 : 6. Thus, the variation of solvent extraction condition could affect the phytochemical content. Further studies about the herbal formula involving the extraction process should concern the variation of extraction conditions to get the highest content of the active compound
Pharmacological Evaluation of a Traditional Thai Polyherbal Formula for Alzheimer’s Disease: Evidence from In Vitro and In Silico Studies
No full textAlzheimer’s disease (AD) is a complex neurodegenerative disorder characterized by multifactorial pathogenesis, including oxidative stress, cholinergic dysfunction, β-amyloid (Aβ) aggregation, and neuroinflammation. In this study, we investigated the neuroprotective potential of the Pheka capsule (PC) formula, a traditional Thai polyherbal medicine comprising Oroxylum indicum (OI), Zingiber officinale (ZO), and Boesenbergia rotunda (BR). Phytochemical analysis by HPLC confirmed the presence of key bioactive compounds including baicalein, baicalin, oroxylin A, 6-gingerol, 6-shogaol, pinocembrin, and pinostrobin. The PC formula exhibited strong antioxidant activity, highly selective butyrylcholinesterase (BChE) inhibition with a selectivity index (SI) of BChE > 20, suppression of Aβ aggregation, and protection against H2O2-induced neuronal damage in vitro. Network pharmacology analysis identified multiple AD-relevant targets and pathways, including APP, GSK3B, CASP3, GAPDH, PTGS2, and PPARG, implicating the PC formula in modulating oxidative stress, apoptosis, and inflammation. Notably, OI emerged as the primary contributor to the formula’s multitargeted actions. These findings support the therapeutic potential of the PC formula as a multitarget agent for AD, aligning with the growing interest in polypharmacological strategies for complex neurodegenerative diseases. Further in vivo and clinical studies are warranted to confirm its efficacy and safety
Cognitive Training Program for Thai Older People with Mild Cognitive Impairment
No full textAbstractPopulation of Thailand is growing rapidly to ageing society. Cognitive abilities change throughout life and decline in ageing depends on the particular type of memory. Cognitive slowing is the change of old age, which affects the individual's capacity to perform activity and quality of life. Mild cognitive impairment (MCI) is a deficit in cognitive function in memory, but does not effect to the daily functioning. However MCI is related in developing to Alzheimer's disease (AD) or other types of dementia commonly found in old age. This research focuses on the elderly people in Thailand with mild cognitive impairment for improving their cognitive function by forming web applications for computers, laptops or tablets. The purpose of this study is to develop a new computer-based program, containing cognitive training software for Thai elderly with mild cognitive impairment. The cognitive training program contains of six categories, involving visuospatial skill, working memory, abstraction, sequencing, categorization and orientation. The validity, reliability, and efficacy of the intervention of the cognitive training program will also investigate. The outcome of using this program will be used for future development of an effective intervention technique for Thai elderly with MCI
Personal and Environmental Well-being of Children in a Thai Suburban Community
No full textAbstractThis study surveyed the personal and environmental well-being of 30 Thai children aged 3-6 years in a suburban community. A questionnaire was examined for content validity, and revealed an acceptable internal consistency (α = 0.73). Results showed that all of the children presented behavior of personal well-being in sleeping for 8-10hours per day, receiving educational preparation, enrolling in school at an appropriate age, and participating with peers. They also presented behavior of environmental well-being by receiving care from, participating in leisure activities with, and obtaining educational support from their family members, as well as living in a peaceful community
Effect of Diacetylcurcumin Manganese Complex on Rotenone-Induced Oxidative Stress, Mitochondria Dysfunction, and Inflammation in the SH-SY5Y Parkinson’s Disease Cell Model
No full textDiacetylcurcumin manganese complex (DiAc-Cp-Mn) is a diacetylcurcumin (DiAc-Cp) derivative synthesized with Mn (II) to mimic superoxide dismutase (SOD). It exhibited superior reactive oxygen species (ROS) scavenging efficacy, particularly for the superoxide radical. The present study investigated the ROS scavenging activity, neuroprotective effects, and underlying mechanism of action of DiAc-Cp-Mn in a cellular model of Parkinson’s disease. This study utilized rotenone-induced neurotoxicity in SH-SY5Y cells to assess the activities of DiAc-Cp-Mn by measuring cell viability, intracellular ROS, mitochondrial membrane potential (MMP), SOD, and catalase (CAT) activities. The mRNA expression of the nuclear factor erythroid 2 p45-related factor (Nrf2), Kelch-like ECH-associated protein 1 (Keap1), inducible nitric oxide synthase (iNOS), and Interleukin 1β (IL-1β), which are oxidative and inflammatory genes, were also evaluated to clarify the molecular mechanism. The results of the in vitro assays showed that DiAc-Cp-Mn exhibited greater scavenging activity against superoxide radicals, hydrogen peroxide, and hydroxyl radicals compared to DiAc-Cp. In cell-based assays, DiAc-Cp-Mn demonstrated greater neuroprotective effects against rotenone-induced neurotoxicity when compared to its parent compound, DiAc-Cp. DiAc-Cp-Mn maintained MMP levels, reduced intracellular ROS levels, and increased the activities of SOD and CAT by activating the Nrf2-Keap1 signaling pathway. In addition, DiAc-Cp-Mn exerted its anti-inflammatory impact by down-regulating the mRNA expression of iNOS and IL-1β that provoked neuro-inflammation. The current study indicates that DiAc-Cp-Mn protects against rotenone-induced neuronal damage by reducing oxidative stress and inflammation
