331 research outputs found
UOrtos [Software]
No full textThe algorithm for co-registration and georeferentation of satellite images assumes that images can be realigned through an isometric transformation. The image alignment is achieved by minimising the distances between common image features. Clustering techniques are used to obtain a high quality set of aligned images without outliers. As a result of the process, the metadata information related to the geotransformation of the satellite images (GeoTIFF) is corrected and co-registered PNG images are derived. The development of this software is aimed for obtaining images to accurately determine shoreline displacements. In general, it can be used for the precise detection of features at different times. Details about the algorithm and methodology are described in Simarro, G.; Calvete, D.; Ribas, F.; Castillo Y.; Puig-Polo, C. UOrtos: Methodology for co-registration and subpixel georeferencing of satellite imagery for coastal monitoring. Remote Sens. 2025, https://doi.org/10.3390/rs3511428UOrtos is an open source software to obtain a set of outlier-free co-registered and georeferenced satellite images with high alignment quality.Peer reviewe
UOrtos: Methodology for Co-Registration and Subpixel Georeferencing of Satellite Imagery for Coastal Monitoring
No full text23 pages, 20 figures, 4 tables.-- Data Availability Statement: Software name: UOrtos. Developers: Gonzalo Simarro, Daniel Calvete. Contact address: [email protected]. Cost: free. License: AGPL-3.0. Availability: https://github.com/Ulises-ICM-UPC/UOrtos (accessed on 19 March 2025). Year first available: 2024. Hardware requirements: PC, server. System requirements: Windows, Linux, Mac. Program language: Python (3.9). Dependencies: OpenCV, NumPy, SciPy and Osgeo modules. Program size: 133 KB. Documentation: README in Github repository and example in an editable Jupyter NotebookThis study introduces a novel methodology for the automated co-registration and georeferencing of satellite imagery to enhance the accuracy of shoreline detection and coastal monitoring. The approach utilizes feature-based methods, cross-correlation, and RANSAC (RANdom SAmple Consensus) algorithms to accurately align images while avoiding outliers. By collectively analyzing the entire set of images and clustering them based on their pixel-pair connections, the method ensures robust transformations across the dataset. The methodology is applied to Sentinel-2 and Landsat images across four coastal sites (Duck, Narrabeen, Torrey Pines, and Truc Vert) from January 2020 to December 2023. The results show that the proposed approach effectively reduces the errors from ∼1 to at least 0.4 px (although they are likely below 0.2 px). This approach can enhance the precision of existing algorithms for coastal feature tracking, such as shoreline detection, and aids in differentiating georeferencing errors from the actual impacts of storms or beach nourishment activities. The tool can also handle complex cases of significant image rotation due to varied projections. The findings emphasize the importance of co-registration for reliable shoreline monitoring, with potential applications in coastal management and climate change impact studiesThis research was funded by Grant TED2021-130321B-I00 funded by MICIU/AEI/10.13039/501100011033 of the Spanish government and by “NextGenerationEU/PRTR” Grants PID2021-124272OB-C21/C22 funded by MCIN/AEI/10.13039/501100011033/ of the Spanish government and by “ERDF A way of making Europe”With the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S)Peer reviewe
Seminar Online | Brazil after Bolsonaro: The Comeback of Lula da Silva
No full textLondon : 10 October 2023, 6:00PM – 7:30PM.Speakers : Richard Bourne (author & editor), Thomas Traumann (journalist & political scientist), Gladys L. Mitchell-Walthour (North Carolina Central University), Luísa Calvete Portela Barbosa (Cardiff University), Jamille Pinheiro Dias (CLACS, ILCS), Gabriel Bayarri (CLACS, ILCS) Contact: [email protected]
Entwicklung neuer massenspektrometrischer Methoden für die Suche, Strukturaufklärung und biologische Untersuchung von peptidischen Toxinen
No full textNatürliche Toxine sind von großer Bedeutung für den Menschen. Ihre biochemischen Eigenschaften resultieren in einem „Ying und Yang“ an tödlichem und zugleich lebensrettenden Potenzial für uns. Etliche pharmazeutische Wirkstoffe basieren auf natürlichen Giften, insbesondere auf peptidischen Toxinen, welche jedes Jahr hunderttausende Menschenleben retten. Im gleichen Moment sind einige dieser Toxine verantwortlich für hunderttausende Todesfälle und fatale gesundheitliche Einschränkungen.
Durch die konstante Verbesserung bio-analytischer und speziell massenspektrometrischer Methoden sind wir auf dem Weg zu einem „kompletten Bild“ an Informationen bezüglich Identität, Konzentration und Konformation bzw. räumliche Interaktionstelle von Naturstoffen oder Toxinen in verschiedenen biologischen Systemen. Diese neuen technologischen Möglichkeiten werden uns zwangsläufig zu einem umfassenden Verständnis von Toxinen und anderen Naturstoffen und ihrer Interaktion mit anderen biologisch relevanten Molekülen führen. Hierdurch werden wir das enorme Reservoir an solchen Verbindungen besser aufdecken und ihr Potential als Modellverbindungen, diagnostische Werkzeuge und schlussendlich als neue therapeutische Wirkstoffe nutzen.
In dieser Arbeit sind die Ergebnisse bezüglich der Suche, Strukturaufklärung, der biochemischen und pharmazeutischen Untersuchung zweier verschiedener Toxin-Klassen, unter Zuhilfenahme neuer massenspektrometrischer Analysemethoden, gezeigt und diskutiert.
Das erste Beispiel handelt von dem nicht-ribosomal synthetisierten Peptid-Polyketid-Hybrid Albicidin, welches von dem Zuckerrohr-Pathogen Xanthomonas albilineans synthetisiert wird. Durch die Sequenzierung des Genoms von Xanthomonas albilineans war es uns möglich die einzigartige biologische Nische und den besonderen Genotyp, innerhalb der Xanthomonaden, welcher etliche ungewöhnliche Biosynthese Gencluster enthält, zu beschreiben. Einer dieser Gencluster ist für die Biosynthese des Phytotoxins Albicidin, einem starken DNA-Gyrase Inhibitor, welcher auch hochwirksam gegen Gram-positive und Gram-negative Bakterien ist, verantwortlich. Die Strukturaufklärung von Albicidin deckte ein bisher unbekanntes chemisches Gerüst auf, welche bis auf Cyano-Alanin und Zimtsäure ausschließlich aus para-Aminobenzoesäure-Derivaten besteht. Die heterologe Produktion und darauf aufbauende in vitro Experimente verschiedener Module der Nicht-Ribosomalen-Peptidsynthetase gab uns eine detaillierte Einsicht in die Biosynthesemaschinerie, was uns half ein umfassendes Biosynthesemodell für Albicidin aufzustellen.
Durch non-targeted Tandem-Massenspektrometrie (MS/MS) konnten wir mehrere Albicidin Derivate, wie z.B. N-terminal carbamoyliertes Albicidin identifizieren. Die Struktur von Carbamoyl-Albicidin konnte durch hochauflösende MS/MS aufgeklärt und die Biosynthese ebenfalls durch heterologe Produktion der Carbamoyltransferase und in vitro Assays sowie einer Geninaktivierungsmutante verifiziert werden.
Die Totalsynthese von Albicidin sowie Carbamoyl-Albicidin ermöglichte uns schließlich die detaillierte Testierung der biologischen Aktivitäten durch in vitro Gyrase-inhibierung und Testierung der antibakteriellen Eigenschaften. Carbamoyl-Albicidin zeigte hierbei stärkere inhibitorische Effekte gegenüber Gyrase als Albicidin, was dafür spricht, dass die Carbamoylierung ein Teil der biosynthetischen Reifung von Albicidin ist.
Der zweite Teil dieser Arbeit handelt von der Einführung und ersten Anwendung von Top-down Massenspektrometrie für die Analyse von Schlangengiften. In einer Machbarkeitsstudie haben wir Gift der Königskobra, Ophiophagus hannah, mit Hilfe einer Top-down Methode analysiert und etliche Toxine verschiedener Proteinfamilien nachgewiesen. Unter diesen Toxinen waren hauptsächlich Drei-Finger-Toxine, ein Kunitz-artiger Proteaseinhibitor, Ohanin, eine bisher unbekannte A2-Phospholipase sowie ein Cystein-reiches-Sekretionsprotein (Ophanin). Eine komplementäre bottom-up Analyse führte zu einem kompletten, Lokus-aufgelösten Venom-Repertoire der Königskobra - der größten bekannten Giftschlange.
Als erstes Beispiel für eine Otter-Spezies haben wir das Venom der Anatolischen Wiesenotter, Vipera anatolica, durch eine Kombination aus Top-down und Bottom-up Venomics analysiert. Durch diese Analyse konnten wir etliche Metalloproteasen, Cystein-reiche-Sekretionsproteine, einen Metalloproteaseinhibitor, A2-Phospholipasen, Disintegrine, eine Serinprotease, Typ-C Lektine sowie einen Kunitz-artigen Proteaseinhibitor identifizieren. Des Weiteren konnten wir etliche Isoformen der oben beschriebenen sowie unbekannten Toxine detektieren, was für eine große, bisher nicht beschriebene Komplexität von Schlangengiften spricht, die mit herkömmlichen bottom-up Techniken unentdeckt geblieben wäre. Schlussendlich konnten wir hierdurch eine bioaktive Komponente des Venoms als ein heterodimeres Disintegrin identifizieren, welches vielversprechende Aktivität gegen Krebszellen zeigte.Natural toxins play an important role for human health. Their biochemical properties result in a “ying and yang” of deadly and lifesaving potential. Several drugs are based on natural and especially peptidic toxins, saving hundred thousands of human lives every year. At the same time, some of these toxins are responsible for hundreds of thousands of deaths and permanent health issues.
With the constant improvement of bioanalytical and especially mass spectrometric techniques, progress has been made towards the accomplishment of “complete pictures” of identities, concentration and spatial location of biomolecules, and in particular toxins, in different biological systems. These technological improvements will enable us to gain a more complete understanding of toxins and all other natural products and their biological interactions. Furthermore these improvements will enable us to better uncover and utilize their enormous potential as biomedical models, diagnostic tools and ultimately novel therapeutics.
In this work, the discovery, structural elucidation, biochemical investigation and finally assessment as potential drugs of two classes of natural toxins are shown and discussed.
The first part is dedicated to the non-ribosomally synthesized peptide-polyketide-hybrid albicidin, which is produced by a sugar cane pathogenic bacterium, Xanthomonas albilineans. By means of the sequencing of the genome of Xanthomonas albilineans we were able to describe its unique biological niche and special genotype among other xanthomonads, containing a number of unusual biosynthetic gene clusters. One of these gene clusters is responsible for the biosynthesis of the phytotoxin albicidin, a potent DNA gyrase inhibitor, which is also highly active against Gram positive and Gram negative bacteria. The structure elucidation revealed the hitherto unknown structure of albicidin, revealing a unique polyaromatic oligopeptide mainly composed of p-amino benzoic acids and cyano-alanine. Heterologous production and in-vitro studies of several domains of the non-ribosomal peptide synthetase rendered detailed insights into the biosynthetic machinery, revealing a comprehensive biosynthetic model for albicidin. Besides the main metabolite, we found several derivatives by non-targeted tandem mass spectrometry (MS/MS) experiments, for example an N-terminally carbamoylated version of albicidin. Carbamoyl-albicidin was characterized by high resolution MS/MS from cultures of X. albilineans and the putative biosynthesis confirmed by gene inactivation of the carbamoyltransferase, heterologous production of the carbamoyltransferase and in vitro reconstitution of the carbamoylation reaction. The chemical synthesis of both albicidin and carbamoyl-albicidin finally enabled us to assess their bioactivities by means of in vitro gyrase inhibition and antibacterial assays. Carbamoyl-albicidin showed stronger inhibitory effects against the gyrase compared to albicidin, which identifies the carbamoylation as an important biosynthetic step of albicidin maturation.
In the second part of this work, the first application of top-down mass spectrometry in snake venomics is shown and discussed. In a case study with venom from king cobra, the application of top-down mass spectrometry enabled us to identify several proteins from different toxin families. Among those toxins are eleven three-finger toxins (7-7.9 kDa), a Kunitz-type inhibitor (6.3 kDa), ohanin (11.9 kDa), a novel phospholipase A2 molecule (13.8 kDa) and a cysteine-rich secretory protein (CRISP) ophanin (25 kDa). Complementary bottom-up MS/MS analyses contributed to complete a locus-resolved venom phenotypic map for Ophiophagus hannah, the world's largest venomous snake.
As a first example for a viperid species we characterized the venom proteome Vipera anatolica, the Anatolian Meadow Viper by a combination of top-down and bottom-up venomics. From this analysis we identified snake venom metalloproteases, cysteine-rich secretory protein isoforms, a metalloprotease inhibitor, several type A2 phospholipases, disintegrins, a snake venom serine protease, a C-type lectin and a Kunitz-type protease inhibitor. Furthermore, we detected several isoforms of above mentioned proteins as well as previously unknown proteins, indicating an extensive complexity of the venom which would have remained undetected with conventional venomic approaches. With these results we could identify one of the bioactive fractions of the venom to be a hetero-dimeric disintegrin, which showed remarkable activity against cancer cells
Novos catalisadores de ródio para hidroformilação com processos ambientalmente sustentáveis
No full textA hidroformilação de olefinas, catalisada por complexos de ródio é o melhor processo de síntese de aldeídos, num só passo e com 100% de economia atómica, tanto ao nível industrial de grande escala como na preparação de compostos do foro da química fina.
No entanto, apesar de que, na atualidade, os catalisadores de ródio são os mais ativos e/ou seletivos, o ródio apresenta um preço muito elevado, sendo que a pesquisa de catalisadores e processos químicos que permitam a reutilização do catalisador é ainda um grande desafio para os químicos orgânicos sintéticos. Para além do desafio económico é também crucial promover o desenvolvimento de processos químicos “amigos do ambiente" que permitam a obtenção dos produtos pretendidos com um mínimo de danos ambientais.
Neste trabalho, foram seguidas duas estratégias distintas tendo em vista a reutilização dos catalisadores de ródio em reações de hidroformilação de olefinas, nomeadamente, utilizando nanopartículas de ródio e sistemas bifásicos envolvendo solventes fluorados.
Ainda no âmbito do desenvolvimento de processos ambientalmente mais inócuos foi desenvolvido um processo auto-tandem que, com o mesmo catalisador de ródio, e sem isolar os aldeídos intermediários, permitiu transformar olefinas em aldeídos, via hidroformilação, seguindo-se a transformação in situ dos aldeídos em álcoois diarílicos por reação destes com ácidos borónicos.
Os estudos iniciaram-se com a síntese de dois monofosfitos quirais derivados do 2,2´-binaftol (BINOL). Este processo iniciou-se com a monoproteção do BINOL com diferentes álcoois. A monoproteção com o álcool benzílico baseou-se na reação de Mitsunobu com a qual foi possível obter o (S)-2’-(benziloxi)-1,1’-binaftilo-2-ol com um rendimento de 86%. Por outro lado, efetuou-se a monoproteção com 4,4,5,5,5-pentafluoropentan-1-ol através do acoplamento de 4,4,5,5,5-pentafluoropentil metanossulfonato com BINOL, obtendo-se o pretendido (R)-2’-(4,4,5,5,5-pentafluoropentiloxi)-1,1’-binaftalen-2-ol com um rendimento de 69%. A preparação dos respetivos monofosfitos foi realizada com algumas modificações ao método clássico da síntese de monofosfitos, nomeadamente, através da reação das três moléculas de BINOL monoprotegidas com tricloreto de fósforo, na presença de trietilamina, que atua simultaneamente como base e como solvente de reação. Após purificação e isolamento foi possível obter o tris[(S)-2’-(benziloxi)-1,1’-binaftilo-2-]fosfito com um rendimento de 51% e o tris[(R)-2’-(4,4,5,5,5-pentafluoropentiloxi)-1,1’-binaftilo-2-]fosfito com um rendimento de 63%. A caracterização dos monofosfitos foi efetuada por espectroscopia de RMN e análise elementar. Posteriormente, estes monofosfitos foram utilizados como ligandos na preparação de catalisadores de ródio e como template na preparação das nanopartículas.
As nanopartículas de ródio com diversos ligandos foram testadas na hidroformilação de 1-dodeceno e estireno. Na hidroformilação do 1-dodeceno, as nanopartículas contendo o tris[(S)-2’-(benziloxi)-1,1’-binaftilo-2-]fosfito como ligando mostraram uma baixa regiosseletividade, obtendo-se uma percentagem ligeiramente superior de aldeído linear (58%) quando comparado com branco da reação. A mesma família de nanopartículas foi testada na hidroformilação do estireno observando-se que neste caso, para além de uma excelente conversão e quimiosseletividade o sistema apresentou também uma elevada regiosseletividade, obtendo-se preferencialmente o aldeído ramificado (87%). Para além deste ligando, foram também testadas nanopartículas contendo dppb- TS, β-ciclodextrina e polivinilpirrolidona (PVP) como estabilizadores em reações de hidroformilação do estireno. O melhor resultado foi obtido com as nanopartículas contendo β-ciclodextrina onde se obteve uma boa conversão (80%) e uma excelente quimio- e regiosseletividade obtendo-se apenas o aldeído ramificado. A reciclagem destas nanopartículas, por filtração, demonstrou uma perda de atividade no segundo ciclo mas manteve-se a mesma excelente quimio- e regiosseletividade. Por outro lado, as nanopartículas contendo PVP demonstraram ser inativas na reação da hidroformilação do estireno nas condições de reação selecionadas.
Os estudos prosseguiram com a avaliação do tris[(R)-2’-(4,4,5,5,5-pentafluoropentiloxi)-1,1’-binaftilo-2-]fosfito como ligando de catalisadores de ródio, com vista à reutilização do catalisador devido à utilização de um sistema bifásico (solvente fluorado/solvente orgânico) em reações de hidroformilação do estireno. A avaliação deste sistema catalítico foi realizada em primeiro lugar no sistema homogéneo, obtendo-se uma elevada conversão (91%) elevada quimioselectividade para a obtenção de aldeídos (99%) e elevada regiosseletividade para a formação do aldeído ramificado (76%). Contudo, o catalisador apresentou baixa enantiosseletividade (15%). Ao utilizar um sistema bifásico de tolueno/perfluoro-1,3-dimetilciclohexano observou-se que a conversão diminuiu para 62% e o ee manteve-se na mesma ordem de grandeza (11%), mas ocorreu um aumento da regiosseletividade para os 86%. Salienta-se que a tentativa de reutilizar o catalisador por separação da fase fluorada revelou um significativo decréscimo da atividade, obtendo-se apenas 7% de conversão, o que indica que o catalisador se encontrava na fase orgânica. Apesar do ee da reação se manter na mesma ordem de valores do primeiro ciclo, verificou-se um aumento substancial da regiosseletividade obtendo-se apenas o aldeído ramificado.Na estratégia que visava a utilização dos aldeídos como intermediários na preparação de álcoois diarílicos através de uma reação tandem, procedeu-se inicialmente à hidroformilação dos substratos trans-anetol e 3-vinil-1H-indol e subsequente arilação, in situ, com ácido fenilborónico e 4-tolilborónico utilizando-se o tris[(S)-2’-(benziloxi)-1,1’-binaftilo-2-]fosfito e a trifenilfosfina como ligandos do ródio. Tanto para o trans-anetol como para o 3-vinil-1H-indol os melhores resultados foram obtidos com o ácido fenilborónico, utilizando a trifenilfosfina como ligando (64% e 58% de álcool, respetivamente)
Methodology for the design of an acid stimulation in carbonate naturally fractured
No full textEn el presente proyecto se desarrolló una metodología para el diseño de trabajos de estimulación ácida en yacimientos naturalmente fracturados; con el fin de remover el daño de formación y aumentar los canales de flujo de las fracturas naturales logrando así un aumento de la producción. Para tal fin se comienza con el estudio de los mecanismos de daño presentes en formaciones de carbonatos y el estudio de la técnica de estimulación viable para este tipo de yacimientos. A continuación se presentan las generalidades del campo P del cual se tomó el pozo P1 como caso para validar la metodología. Después se propone la metodología iniciando con la selección de candidatos la cual nos permitirá determinar a partir de criterios operacionales de potencial de producción, los pozos viables para implementar un trabajo de estimulación ácida. Posteriormente dentro del diseño básico de los trabajos de estimulación, sé propone una etapa cuyo objetivo será predecir la distancia de penetración del tratamiento ácido dentro de la fractura natural de la formación, con esta distancia y las dimensiones de la fractura se podrá calcular el volumen de ácido requerido para el tratamiento. Luego se implementa la metodología desarrollada, en un pozo. Por último se valida la metodología comparando los resultados obtenidos de un tratamiento real realizado al pozo P1 y los resultados que se obtendrían si se utilizara la metodología propuesta en estePregradoIngeniero de PetróleosWe developed a methodology to design works on acid stimulation in naturally fractured reservoirs. We did it to remove damage formation and to increase flux channels of natural fractures, in order to increase production. We start studying damage mechanisms present in carbonate formations, and studying stimulation techniques feasible to this type of reservoirs. Generalities of camp P are showed and oil well P1 was chosen to validate this methodology. Then, we propose a methodology by selecting candidates that will allow us to determinate, based on operational criteria of production potentiality, feasible oil wells to implement a work on acid stimulation. Then, we propose a phase directed to predict penetration distance of acid treatment inside the natural formation fracture, within the basic design of stimulation works. Acid volume required for this treatment will be estimated based on this distance. We then test the developed methodology on an oil well. Finally, we validate this methodology by comparing results from a real treatment performed in oil well P1, and results that would be obtained under the methodology proposed in thi
UCalib: Cameras Autocalibration on Coastal Video Monitoring Systems
No full textFollowing the path set out by the “Argus” project, video monitoring stations have become a very popular low cost tool to continuously monitor beaches around the world. For these stations to be able to offer quantitative results, the cameras must be calibrated. Cameras are typically calibrated when installed, and, at best, extrinsic calibrations are performed from time to time. However, intra-day variations of camera calibration parameters due to thermal factors, or other kinds of uncontrolled movements, have been shown to introduce significant errors when transforming the pixels to real world coordinates. Departing from well-known feature detection and matching algorithms from computer vision, this paper presents a methodology to automatically calibrate cameras, in the intra-day time scale, from a small number of manually calibrated images. For the three cameras analyzed here, the proposed methodology allows for automatic calibration of >90% of the images in favorable conditions (images with many fixed features) and ∼40% in the worst conditioned camera (almost featureless images). The results can be improved by increasing the number of manually calibrated images. Further, the procedure provides the user with two values that allow for the assessment of the expected quality of each automatic calibration. The proposed methodology, here applied to Argus-like stations, is applicable e.g., in CoastSnap sites, where each image corresponds to a different camera
UBathy (v2.0): A Software to Obtain the Bathymetry from Video Imagery
No full textUBathy is an open source software developed for bathymetry estimation from video images. The proposed scheme is based on extracting the wave modes from videos of the nearshore surface wave propagation. These videos can be formed either from raw camera images, which must have been previously calibrated, or from georeferenced planviews. For each wave mode extracted from the videos, the wave frequency and the spatially dependent wavenumbers are obtained. The frequencies and wavenumbers from different videos are used to estimate the bathymetry by adjusting the dispersion relationship for linear surface water waves. The bathymetry at different times can further be weighted and aggregated using the Kalman filter. The new software is suitable for Argus-type video monitoring stations and for moving cameras mounted on drones or satellites, and it is meant for users familiar with coastal image processing and suitable for non-experienced users. The software and an application example are available on the GitHub platform
Study technical and financial feasibility for application of the system artificial lift chamber lift in the colorado school field.
No full textEl propósito de éste estudio es realizar un muestreo de los pozos del Campo Escuela Colorado y seleccionar aquellos que cuentan con los parámetros óptimos para la implementación del sistema de levantamiento artificial Chamber Lift con el objetivo de estudiar la viabilidad tanto técnica como financiera de implementar este sistema como método de levantamiento de crudo en dicho campo. Analizando las variables que inciden en el desempeño del sistema Chamber Lift y teniendo en cuenta que el principio del sistema se basa en la energía que acumula y aporta el gas que se encuentra en el espacio anular de la cámara, se determinó que las principales variables que imposibilitan la implementación del sistema en un pozo, son las aquellas variables inherentes al comportamiento del fluido como son la presión de fondo fluyendo (Pwf) y la relación Gas-Liquido (GLR). Con la información suministrada por parte del área de producción del campo se seleccionaron los pozos que cuentan con las propiedades dentro de los rangos aceptables para realizarles el diseño del sistema Chamber Lift, encontrando así tres pozos candidatos para realizar el estudio dentro de los pozos activos en la actualidad. Por otro lado se realizó un análisis financiero a partir de la producción lograda con la implementación del sistema Chamber Lift en los pozos seleccionados, basados en el cálculo del Valor Presente Neto (VPN), la Tasa Interna de Retorno (TIR) y el tiempo de recuperación de la inversión (Payback) encontrando rentable la implementación del sistema en dos de los tres pozos seleccionados ya que se obtuvo un VPN positivo lo que indica que se logra cubrir la inversión inicial y por otro lado la TIR es mayor a la tasa de oportunidad del campo evidenciando que se generan ganancias.PregradoIngeniero de PetróleosThe purpose of this study is to sample wells Colorado School Field and select those with the optimal parameters for implementing artificial lift system Lift Chamber in order to study the technical and financial feasibility of implementing this system as rising crude method in this field. Analyzing the variables that affect system performance Lift Chamber and considering that the principle of the system is based on the energy that builds up and brings the gas in the annulus of the camera, it was determined that the main variables preclude the implementation of the system in a well, those are the variables inherent in the behavior of the fluid such as the flowing bottomhole pressure (PWF) and gas-liquid ratio (GLR). With the information provided by the área of field production wells were selected that have properties within acceptable ranges unit to perform system design Chamber Lift, finding three wells candidates for the study within the active wells in present. Furthermore financial analysis was performed from the production achieved by implementing Chamber Lift System in selected wells, based on the calculation of the Net Present Value (NPV), Internal Rate of Return (IRR) and the time Payback (Payback) finding profitable system implementation in two of the three wells selected inasmuch as NPV was positive indicating that manages to cover the initial investment and moreover the IRR is greater than the rate of opportunity field generated showing gains
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