188,172 research outputs found
Io. Diominici Cosciae... Dissertationes iurium : in quibus inuenies digestos tractatus... : cum copiosissimis omnium rerum indicibus...
No full textEste ejemplar repite los últimos cuadernillos [ ]\p4\s, B-E\p4\s, [cruz]\p6\s, que se corresponden con: "Ioannis Dominici Cosciae... responsum primum... secundum... tertium"Marca tip. en portSign.: [ ]\p2\s, A-M\p6\s, N\p5\s, O-R\p4\s, A-C\p4\s, A-R\p4\s, [parágrafo]-3[parágrafo]\p4\s, [ ]\p2\s, A-I\p6\s, [ ]\p4\s, B-E\p4\s, 2[cruz latina]\p6\sTexto a dos colPort. a dos tintasSign. : []¡2, A-M¡6, N¡5, O-R¡4, A-C¡4, A-R¡4, Ú-3Ú¡4, []¡2, A-I¡6, []¡4, B-E¡4, 2[cruz latina]¡6Con portadilla propia : "Responsum de praecedentia inter lectorem cathearae Iuris Ciuilis ... & lectorem Iuris Canonici ... in studio Neapolitano / pro D. Emanuele Roderico Nauarro, lectore dictae cathedrae Iuris Ciuilis aduersus D. Cosciam lectorem Iuris Canonici", p. 159"Antipologia aduersus veritatis impugnatores & iniquitatis defensores / [P]ascalis de Orco ... Io. Dominici Cosciae ... ", pag. propia"Ioannis Dominici Cosciae ... responsum primum, in materia praecedentiae aedita in controuersia ... inter ipsum ... Cosciam & ... Emanuelem Nauarrum""Ioannis Dominici Cosciae ... Responsum secundum, in quo adducuntur rationes dubitandi quibus vespertinus lector sit praeferendus iuris pontificij lectori, & ipsius aduersarij oppositiones veris solutionibus resoluuntur""Ioannis Dominici Cosciae ... Responsum tertium in quo traduntur rationes quae pra lectore iuris pontificij faciunt, & aduersariorum responsiones confutantur"Con portadilla propia : "Responsum de praecedentia inter lectorem cathedrae Iuris Ciuilis... & lectorem Iuris Canonici... in studio Neapolitano / pro D. Emanuele Roderico Nauarro, lectore dictae cathedrae Iuris Ciuilis aduersus D. Cosciam lectorem Iuris Canonici", p. 159. "Antipologia aduersus v eritatis impugnatores & iniquitatis defensores / [P]ascalis de Orco... Io. Dominici Cosciae...", pag. propia; "Ioannis Dominici Cosciae... responsum secundum, in quo adducuntur rationes dubitandi quibus vespertinus lector sit praeferendus iuris pontificij lectori, & ipsius aduersarij oppositione -- "Ioannis Dominici Cosciae... Responsum tertium in quo traduntur rationes quae pro lectore iuris pontificij faciunt, & aduersariorum responsiones confutantur
My first fifty years in ionospheric research
No full textThe author, as an active witness, recalls what he has seen of the development of ionospheric physics during the last 50 years.JCR Journalope
Monumenta Ordinis Fr. Praedicatorum Historica. Tomus XV: Monumenta Historica S. P. N. Dominici. Fasc. I: Historia Diplomatica S. Dominici, recensuit Fr. M.Hyacintus Laurent
No full textRanwez Charles. Monumenta Ordinis Fr. Praedicatorum Historica. Tomus XV: Monumenta Historica S. P. N. Dominici. Fasc. I: Historia Diplomatica S. Dominici, recensuit Fr. M.Hyacintus Laurent. In: Revue néo-scolastique de philosophie. 38ᵉ année, Deuxième série, n°47, 1935. pp. 397-398
Monumenta Ordinis Fr. Praedicatorum Historica. Tomus XV: Monumenta Historica S. P. N. Dominici. Fasc. I: Historia Diplomatica S. Dominici, recensuit Fr. M.Hyacintus Laurent
No full textRanwez Charles. Monumenta Ordinis Fr. Praedicatorum Historica. Tomus XV: Monumenta Historica S. P. N. Dominici. Fasc. I: Historia Diplomatica S. Dominici, recensuit Fr. M.Hyacintus Laurent. In: Revue néo-scolastique de philosophie. 38ᵉ année, Deuxième série, n°47, 1935. pp. 397-398
Mutation of cysteine 111 in dopa decarboxylase leads to active site perturbation
No full textCysteine 111 in Dopa decarboxylase (DDC) has been replaced by alanine or serine by site-directed mutagenesis. Compared to the wild-type enzyme, the resultant C111A and C111S mutant enzymes exhibit k(cat) values of about 50% and 15%, respectively, at pH 6.8, while the K(m) values remain relatively unaltered for L-3,4-dihydroxyphenylalanine (L-Dopa) and L-5-hydroxytryptophan (L-5-HTP). While a significant decrease of the 280 nm optically active band present in the wild type is observed in mutant DDCs, their visible co-enzyme absorption and CD spectra are similar to those of the wild type. With respect to the wild type, the Cys-111→Ala mutant displays a reduced affinity for pyridoxal 5'-phosphate (PLP), slower kinetics of reconstitution to holoenzyme, a decreased ability to anchor the external aldimine formed between D-Dopa and the bound co-enzyme, and a decreased efficiency of energy transfer between tryptophan residue(s) and reduced PLP. Values of pK(a) and pK(b) for the groups involved in catalysis were determined for the wild-type and the C111A mutant enzymes. The mutant showed a decrease in both pK values by about 1 pH unit, resulting in a shift of the pH of the maximum velocity from 7.2 (wild-type) to 6.2 (mutant). This change in maximum velocity is mirrored by a similar shift in the spectrophotometrically determined pK value of the 420 → 390 nm transition of the external aldimine. These results demonstrate that the sulfhydryl group of Cys-111 is catalytically nonessential and provide strong support for previous suggestion that this residue is located at or near the PLP binding site (Dominici P, Maras B, Mei G, Borri Voltattorni C. 1991. Eur J Biochem 201:393-397). Moreover, our findings provide evidence that Cys-111 has a structural role in PLP binding and suggest that this residue is required for maintenance of proper active- site conformation.Cysteine 111 in Dopa decarboxylase (DDC) has been replaced by alanine or serine by site-directed mutagenesis. Compared to the wild-type enzyme, the resultant C111A and C111S mutant enzymes exhibit k(cat) values of about 50% and 15%, respectively, at pH 6.8, while the K-m values remain relatively unaltered for L-3,4-dihydroxyphenylalanine (L-Dopa) and L-5-hydroxytryptophan (L-5-HTP). While a significant decrease of the 280 nm optically active band present in the wild type is observed in mutant DDCs, their visible co-enzyme absorption and CD spectra are similar to those of the wild type. With respect to the wild type, the Cys-111-->Ala mutant displays a reduced affinity for pyridoxal 5'-phosphate (PLP), slower kinetics of reconstitution to holoenzyme, a decreased ability to anchor the external aldimine formed between D-Dopa and the bound co-enzyme, and a decreased efficiency of energy transfer between tryptophan residue(s) and reduced PLP. Values of pK(a) and pK(b) for the groups involved in catalysis were determined for the wild-type and the C111A mutant enzymes. The mutant showed a decrease in both pK values by about 1 pH unit, resulting in a shift of the pH of the maximum velocity from 7.2 (wild-type) to 6.2 (mutant). This change in maximum velocity is mirrored by a similar shift in the spectrophotometrically determined pK value of the 420 --> 390 nm transition of the external aldimine. These results demonstrate that the sulfhydryl group of Cys-111 is catalytically nonessential and provide strong support for previous suggestion that this residue is located at or near the PLP binding site (Dominici P, Maras B, Mei G, Borri Voltattomi C. 1991. fur J Biochem 201:393-397). Moreover, our findings provide evidence that Cys-Ill has a structural role in PLP binding and suggest that this residue is required for maintenance of proper active-site conformation
The Holonic Production System: a Multi Agent Simulation Approach
Get PDFToday’s turbulent markets are facing unpredictable and sudden variations in demand. In this context, the Holonic Production System (HPS) seems to be able to overcome the operational and economic problems of traditional production
systems. The HPS’ ability to adapt and react to business environment changes, whilst maintaining systemic synergies
and coordination, leverage on its network organizational structure, assuring both flexibility and profitability. In this
paper we study HPS experimentally, modeling holon-firms as agents. In our simulation, holon-firms interact both with
each other and with the external environment without predetermined hierarchies and following their own aims and internal decision rules with a negotiation-based control system. The Multi Agent System Approach we propose aims to
evaluate and test the performance of the HPS to adjust to changes in market demand by simulating variations in
holon-firms’ capacity and reconfiguration costs in real time in a distributed enterprise network. Hence we demonstrate
that, through a collaborative negotiation approach, the HPS results in a better adaptability and improved network responsiveness
2499. Miracula S. Dominici Rothomagi
No full text2499. Miracula S. Dominici Rothomagi. In: Molinier Auguste. Les Sources de l'histoire de France - Des origines aux guerres d'Italie (1494). III. Les Capétiens, 1180-1328. Paris : A. Picard et fils, 1903. p. 78
MUJER Y SALUD REPRODUCTIVA EN EL MUNICIPIO DE KAUA (YUCATAN)
No full textEl presente trabajo analiza las trayectorias reproductivas de las mujeres embarazadas que viven en el municipio de Kaua, Yucatán (México). En el municipio, como en todo el Estado, hay una oferta plural en salud: la mujer embarazada es atendida tanto por profesionales de la salud como por parteras locales, que efectúan sobadas (mansajes para "reubicar" al bebé) cada mes. Parir en el hospital y parir en la casa son dos opciones concretas y accesible para las mujeres mayas. La elección tiene que ver con distintos aspectos, entre éstos el compartir o no una visión medicalizada del parto y del nacimiento
Reaction of Dopa decarboxylase with alfa-methylDopa leads to an oxidative deamination producing 3,4-dihydroxyphenylacetone, an active-site directed affinity label
No full textDopa decarboxylase (DDC) catalyzes the cleavage of alpha-methylDopa into 3,4-dihydroxyphenylacetone and ammonia, via the intermediate alpha-methyldopamine, which does not accumulate during catalysis. The ketone has been identified by high-performance liquid chromatography and mass spectroscopic analysis, and ammonia by means of glutamate dehydrogenase. Molecular oxygen is consumed during the reaction in a 1:2 molar ratio with respect to the products. The kcat and Km of this reaction were determined to be 5.68 min-1 and 45 microM, respectively. When the reaction is carried out under anaerobic conditions, alpha-methyldopamine is formed in a time-dependent manner and neither ammonia nor ketone is produced to a significant extent. The reaction is accompanied by a time- and concentration-dependent inactivation of the enzyme with kinact of 0. 012 min-1 and Ki of 39.3 microM. Free 3,4-dihydroxyphenylacetone binds to the active site of DDC and inactivates the enzyme in a time- and concentration-dependent manner with a kinact/Ki value similar to that of alpha-methylDopa. d-Dopa, a competitive inhibitor of DDC, protects the enzyme against inactivation. Taken together, these findings indicate the active site directed nature of the interaction of DDC with 3,4-dihydroxyphenylacetone and provide evidence that the ketone generated by the reaction of DDC with alpha-methylDopa dissociates from the active site before it inactivates the enzyme. Inactivation of the enzyme by ketone followed by NaB3H4 reduction and chymotryptic digestion revealed that the lysine residue which binds pyridoxal 5'-phosphate (PLP) in the native enzyme is the site of covalent modification. Together with the characterization of the adduct released from the inactivated DDC, these data suggest that the enzyme is inactivated by trapping the coenzyme in a ternary adduct with ketone and the active site lysine. As recently reported for serotonin (5-HT) [Bertoldi, M., Moore, P. S., Maras, B., Dominici, P., and Borri Voltattorni, C. (1996) J. Biol. Chem. 271, 23954-23959], the conversion of dopamine (DA) into 3,4-dihydroxyphenylacetaldehyde and ammonia catalyzed by DDC is accompanied by irreversible loss of decarboxylase activity. However, the comparison between the absorbance, fluorescence, and CD features of DDC after 5-HT- or 3, 4-dihydroxyphenylacetone-induced inactivation shows that a different covalent adduct is formed between either of these two molecules and DDC-bound PLP
L'Affaire Dominici : P. Bresson, R. Follet
No full textLes rencontres entre le droit et la bande dessinée sont décidément riches! L'ouvrage scénarisé par Pascal Bresson et dessinée par René Follet, annoncé dans un précédent billet, permet d'offir un point de vue intéressant sur la célèbre affaire Dominici. Les règles juridiques ne sont absolument pas abordées, mais il est difficile de passer à côté d'une affaire à laquelle la Cour de cassation elle-même a consacré une conférence. Formellement, la bande dessinée est très agréable : le dessin repre..
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