196,064 research outputs found
SURGERY IN THE VERY OLD PATIENT - EVALUATION OF FACTORS LINKED TO POSTOPERATIVE MORBIDITY AND MORTALITY
No full textCombination of copeptin and highly sensitive troponin I for diagnosing acute myocardial infarction at emergency department admission.
No full textCombination of copeptin and highly sensitive troponin I for diagnosing acute myocardial infarction at emergency department admission
Relationship between red blood cell distribution width and prognostic biomarkers in patients admitted to the emergency department with acute infections.
No full textRelationship between red blood cell distribution width and prognostic biomarkers in patients admitted to the emergency department with acute infections
Development of a high-performance affinity chromatography-based method to study the biological interaction between whole microorganisms and target proteins
No full textAnalytical assessment of the Beckman Coulter Unicel DxI AccuTnI+3 immunoassay.
No full textThe aim of this study (performed between December the 2nd and the 12th, 2013) was the analytical evaluation of the novel AccuTnI+3 on Unicel DxI, along with evaluation of carry-over
Influence of hemolysis on troponin testing: studies on Beckman Coulter UniCel Dxl 800 Accu-TnI and overview of the literature.
No full textBackground: Hemolyzed specimens are the leading preanalytical problem in the laboratory practice, and exert a negative impact on test results. We assessed the reliability of Beckman Coulter UniCel Dxl 800 Accu-TnI testing on hemolyzed specimens. Methods: Twelve non-hemolyzed K2EDTA- anticoagulated samples displaying Accu-TnI values >0.20 μg/L and nine with values <0.04 μg/L were selected and three aliquots were obtained from each. The first ("#A") was processed without further manipulation, whereas the second ("#B") and third ("#C") were hemolyzed by aspirating anticoagulated blood through a thin needle. Plasma was separated and tested for hemolysis index (HI) and Accu-TnI. Results: As compared with aliquot #A (HI: 0), a progressive increase of hemolysis occurred in aliquots #B (HI: 25) and #C (HI: 45). The concentration of Accu-TnI gradually decreased from aliquots #A (0.89 μg/L, 0.20-20.16 μg/L), to aliquots #B (0.81 μg/L, 95% CI 0.17-18.37 μg/L; ps0.041) and #C (0.78 μg/L, 95% CI 0.15-17.48 μg/L; ps0.026). In 0/12 (aliquots #B) and 3/12 cases (aliquots #C) the percent decrease exceeded 20% variation. The values remained unchanged in nine samples with Accu-TnI -0.04 μg/L. Conclusions: Accu-TnI values decrease in hemolyzed samples, but the bias might not be clinically significant in samples with hemoglobin <14.5 g/L. © 2011 by Walter de Gruyter
Chitosan elicits mono-glucosylated stilbene production and release in fed-batch bioreactor cultures of grape cells
No full textThe present paper reports the study of the optimal conditions for grape (Vitis vinifera cv. Barbera) cell suspensions
in batch and fed-batch bioreactor cultures, in order to specifically improve the production of
mono-glucosylated stilbenes, which are resveratrol derivatives. These compounds are physiologically
as active as free resveratrol in cardio- and chemoprotection, but are more stable and bioavailable after
ingestion through diet. In fed-batch conditions the production of mono-glucosides was considerably
increased together with that of free resveratrol. For the first time, an elicitor (chitosan) was tested in a
bioreactor system, demonstrating its efficacy in inducing the production of stilbenes. The bioreactor culture
conditions also allowed the accumulation of other polyphenols, such as catechins. The vast majority
of the produced compounds was released into the culture media, which represents a relevant advantage
for the recovery of specific molecules or of polyphenol-enriched mixtures. These results represent a further
step toward the employment of grape cells in fed-batch cultures, as a promising alternative to whole
plant extraction for the industrial production of plant polyphenols, considering the necessity for developing
sustainable processes
Indicazioni delle tecniche da associare agli interventi di artrorisi temporanea della sottoastragalica.
No full textRed Blood Cell distribution width and erythrocyte parameters in patients with brain injury after mild head trauma.
No full textRed Blood Cell distribution width and erythrocyte parameters in patients with brain injury after mild head trauma
Biodiversity among sheep breeds to produce sukling lamb. 2. Meat quality characteristics
No full textThe aim of the present research was to evaluate the physical and chemical characteristics of
meat of twenty-four suckling lambs, 12 Leccese lambs (LEC) and 12 Comisana lambs (COM)
fed with ewe milk from mothers (NAT) or artificial milk (ART) and slaughtered at 35 days of
age. Leccese meat from suckling lamb was significantly higher in water content (74.91% vs
73.76%) and lower in fat content (2.86% vs 3.75%). No significant differences were observed
between the two breeds and feeding system on meat tenderness, but Leccese lambs, fed with artificial milk, gave more tender meat (2.02 kg/cm2 vs 2.60, kg/cm2). Regarding the colour
characteristics differences on Longissimus lumborum muscle were observed on lightness (L*)
resulting significantly higher in suckling Leccese lambs compared to Comisana lambs (42.03
vs 41.29; P<0.05). A lower red index (a*) (8.02 vs 8.53; P<0.05) and a higher yellow index
(b*) (8.83 vs 8.56; P<0.01) were found in Leccese breed lambs comparing to Comisana.
Fatty acid profile of the meat showed a significant (P<0.05) higher incidence of saturated
fatty acid in Naturale group (43.72%) comparing with Artificiale group (35.83%).
Monounsaturated fatty acids in artificial diet compared to natural (54.40% vs 46.79%) and
those of Comisana breed (P<0.05) comparing to Leccese (51.45% vs 49.74%) were higher.
Atherogenicity and thrombogenicity indexes were better in suckling lamb fed with artificial
milk (P<0.01). In Leccese lambs the better ratio ω6/ω3 was obtained when lambs were fed
with ewe milk comparing to artificial (4.01 vs 5.50; P<0,05).
Cholesterol content in the blood and liver was lower (P<0.01) in lambs fed with artificial milk in both breeds
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