1,720,987 research outputs found

    Clinical evaluation of poor milking procedures effects on dairy Mediterranean buffaloes udder health

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    Two pluriparous [MB1: 243 days in milk (DIM); MB2: 232 DIM] and 1 primiparous (MB3: 247 DIM) Mediterranean Buffaloes (MB), were submitted to clinical examination of teat and udder including CMT, sterile milk sampling for bacteriological milk culture (BMC) and somatic cells count (SCC) evaluation. Milking procedures monitoring and teat scoring of all milking MB were also performed. MB1 showed apical teat trauma with ulceration near orifice and a moist, wet, inflamed area surrounding the teat canal; MB2 revealed vertical skin fissure originating from the teat apex and flowing into a wide moist and wet area closer to the base of the teat with signs of skin necrosis. Finally MB3 revealed severe teat canal eversion with dry keratinized protrusion, grade 4. MB1 and MB3 were score CMT +2, instead MB3 +3. BMC revealed E. coli mono-infections in MB1 and 3, with SCC of 152^104 and 119^104 respectively; milk sample of MB3 was instead sterile with 450^104 SCC. A hygiene score, ranged from 1 (clean) to 5 (dirty,) was also assessed for 5 body areas, with the following mean results: tail head 3.0±0.9 (score ± standard deviation), thigh (lateral aspect) 3.3±0.5, abdomen 2.7±0.5, udder 3.7±0.5, rear limbs 4.3±0.5. According to the clinical picture and the weak point were observed during milking procedures, teat lesions due to poor milking procedures have been suspected. A correct therapy and strategies to prevent the problem in future have been suggested. All the indications given must to be included in complete and up-date udder health monitoring program, allowing to correctly monitor over-time milk yield and quality, guaranteeing to detected presence of problem as soon as possible and aiming to reduce their short-, mid- and long- term negative effects

    Validation of a pedometer algorithm as a tool for evaluation of locomotor behaviour in dairy Mediterranean buffalo.

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    This research communication of technical interest addresses the goal to validate an algorithm to monitor natural occurrence of locomotor behaviours in dairy Mediterranean buffalo based on the output of a 3-dimensional accelerometer (RumiWatch®, pedometer). Several characteristics of the locomotor behaviour were detected with a very high (up-right, lying and standing time) or high degree of correlation (walking time and number of strides) and a low mean difference with the video recording. The proportion of correctly detected events exceeded 99% for the following variables: stand up and lie down events, as well as number of lying, standing or walking bouts. The mean relative measurement error was less than 10% for the variables: lying, standing, up-right times and number of strides as compared with gold standard. This new algorithm may represent the base for a future early and real-time disease warning system aiming to gain higher health standard in these ruminants

    Plasma Atrial Natriuretic Peptide (proANP 31-67), B-type Natriuretic Peptide (Nt-proBNP) and endothelin (ET-1) concentrations in dogs with chronic degenerative valvular disease (CDVD).

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    In congestive heart failure neuroendocrine network activation is complex and is generally associated with worsening of hemodynamic status and severity of clinical findings. The aim of this research is to study Atrial Natriuretic Peptide (proANP), Brain Natriuretic Peptide (Nt-proBNP) and Endothelin-1 (ET-1) plasma concentrations in dogs affected by chronic degenerative valvular disease (CDVD) classified using the International Small Animal Cardiac Health Council functional scheme (ISACHC). CDVD was diagnosed in twenty-nine mixed, and pure breed, dogs on the basis of clinical examination, thoracic radiography, electrocardiography and echocardiography. Commercial enzyme-linked immunoassays validated for canine species were used to measure the neuroendocrine markers. Nt-proBNP and proANP levels increase in proportion to the severity of heart failure, although there was no statistical difference in the ANP plasma levels between ISACHC classes II and III. Significant difference in BNP plasma levels was observed among all the heart failure classes. Plasma ET-1 concentration increased in classes II and III compared to that of class Ib, but the increment was not progressive and no statistical differences were found between the different classes of heart failure considered. In conclusion, under our experimental conditions Nt-proBNP plasma levels were shown to be more useful than other cardiac biomarkers in assessing the severity of heart failure in dogs with CDVD, classified according to the ISACHC functional scheme

    Cardiac biomarkers in clinical practice of dog and cat - a review

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    A biomarker is an indicator of a normal or pathologic process, or a pharmacologic response to a therapeutic intervention. Nowadays, in veteriary cardiology, the most used biomarkers for assessing the pathological status of the cardio-vascular system, are B-type natriuretic peptide and cardiac troponins. These biomarkers have been very well studied in cardiac pathology in order to assess their diagnostic potential. The aim of the present paper was to discuss the structure, metabolism, function, applicability, reference values and variations in different diseases and to review some practical aspects of the two cardiac biomarkers, used nowadays in small animal cardiology

    Diagnostic imaging procedures and herd management algorithm in grazing cows affected by chronic enzootic hematuria.

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    Introduction - Chronic Enzootic Hematuria (CEH) is a bladder disorder due to bracken fern intake and observed in grazing cows of south Italy. Modern imaging techniques such as ultrasound and cystoscopy can be recently applied in alive animals to perform diagnosis. Aim - The goals of present investigation were: to compare the usefulness of ultrasonography and video-cystoscopy in animals affected by CEH, to evaluate the impairment of animal welfare due to the procedures performed, to develop a new diagnostic/decision algorithm based on the analysis of the previous goals. Materials and Methods - After a screening phase performed on 230 Podolic breed cows, 24 cows with hematuria were enrolled. All of them were scored for discomfort status pre- and post- ultrasonography (T0u and T1u, respectively), as well as pre- and post- cystoscopy (T0c and T1c) by means of cumulative scoring system. Results - Ultrasonography allows an easy and early identification of structural changes of the organs involved with low consequences on animals’ welfare; video-cystoscopy guaranteed a direct vision of the bladder’s mucosa and lesions as well as the possibility of biopsy in field achieving a certain diagnosis and staging. Comparing the two techniques (T1u vs. T1c), significant difference were observed for the variables aimless chewing (P<0.05), response to approach (P<0.01) as well as for behaviors such us attention, back position weight shifting, ear position (P<0.0001) and the parameters HR and RR (P<0.0001). Discussion and Conclusions – Independently from disease's severity, ultrasonography was perceived as less stressful by the cows when compared with a video-cystoscopy entailing longer execution times and higher level of animal handling. The combined use of the two imaging techniques produced a diagnostic/decisional algorithm that may aid the practitioners in field to manage the animals affected. Herd grazing in endemic areas should be monitored on regular base and presence of one positive cows should justify further investigations

    Penicillin-Resistant Aerococcus viridans Bacteremia Associated with Bovine Severe Respiratory Syndrome

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    Aerococcus viridans, a less frequently isolated bacteria, is a gram-positive, catalase-negative coccus, found singly or in tetrads, with biochemical and growth characteristics of streptococci and enterococci. This microorganism, usually susceptible to penicillin, is often found in the environment and is infrequently associated with human/veterinary infections. We described a case of Holstein Friesian female calf, 150-day-old, affected by respiratory emergencies. Following the clinical signs, radiographic analysis and bacteriological/molecular examinations carried out on blood culture, a diagnosis of severe broncho-pulmonary disease associate with a multidrug-resistant A. viridans bacteremia was done. The present case highlights the invasive nature of a saprophytic bacterium showing a broad profile of antibiotic-resistance including β-lactams. Furthermore, this report confirms that the effectiveness of an antibiotic therapy is based primarily on a sure diagnosis including susceptibility testing

    Marker biochimici del metabolismo osteo-minerale in bufale con turbe locomotorie croniche riferibili ad osteomalacia (“sciatica del bufalo”)

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    Nel presente studio sono state selezionate 34 bufale in asciutta di età compresa tra i 10 - 18 anni, di cui 24 affette da turbe locomotorie riferibili a osteomalacia cronica (cosiddetta sciatica del bufalo) e 10 bufale sane (gruppo di controllo). In questi animali sono stati determinati i seguenti parametri del turnover osteo-minerale: calcio, fosforo e magnesio totali, creatinina, urea, proteine totali, aspartato aminnotrasferasi, creatininfosfochinasi, fosfatasi alcalina, gamma glutamin transferasi, paratormone, calcitonina ed ostiolacina sierica. Gli animali ammalati hanno mostrato elevate concentrazioni di PHT e ALP nonché ridotti livelli di fosforo, calcio e ostiocalcina sierica se paragonati al gruppo di controllo
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