1,721,010 research outputs found
Genome methylation of the marine annelid worm Chaetopterus variopedatus:methylation of a CpG in an expressed H1 histone gene.
No full textOccurrence of genetic exchange in the environment between natural and laboratory microorganisms:plasmids in psichrophilic bacteria of italian natural spring waters.
No full textCharacterization of a new variant DNA (cytosine-5)-methyltranferase unable to methylate double stranded DNA isolated from the marine annelid worm Chaetopterus variopedatus.
No full textThe enzyme S-adenosylmethionine-DNA (cytosine-5)-methyltransferase has been identified, first time for invertebrates, in embryos of the marine polychaete annelid worm Chaetopterus variopedatus. The molecule has been isolated from embryos at 15 h of development. It is a single peptide of about 200 kDa molecular weight, cross-reacting with antibodies against sea urchin DNA methyltransferase. The enzymatic properties of the molecule are similar to those of Dnmt1 methyltransferases isolated from other organisms, but with the peculiarity to be unable to make 'de novo' methylation on double stranded DNA. Copyright (C) 1999 Federation of European Biochemical Societies
GNG5 Controls the Number of Apical and Basal Progenitors and Alters Neuronal Migration During Cortical Development
Full text linkCortical development is a very complex process in which any temporal or spatial alterations can give rise to a wide range of cortical malformations. Among those malformations, periventricular heterotopia (PH) is characterized by clusters of neurons that do not migrate to the correct place. Cerebral organoids derived from patients with mutations in DCHS1 and FAT4, which have been associated with PH, exhibit higher levels of GNG5 expression in a patient-specific cluster of neurons. Here we investigate the role of GNG5 during the development of the cerebral cortex in mice and human cerebral organoids. GNG5, highly expressed in progenitors and downregulated in neurons, is critical for controlling the number of apical and basal progenitors and neuronal migration. Moreover, forced expression of GNG5 recapitulates some of the alterations observed upon downregulation of Dchs1 and Fat4 in mice and human cerebral organoids derived from DCHS1 and FAT4 patients, suggesting a critical role of GNG5 in cortical development
DNA (CYTOSINE-5) METHYLTRANSFERASE TURNOVER AND CELLULAR LOCALIZATION IN DEVELOPING P. LIVIDUS SEA URCHIN EMBRYO.
No full textInfluences of Wolbachia (Rickettsiales Rickettsiaceae) on the cellular response to cold streb in Drosophila melanogaster (Diptera Drosophilidae)
No full textWolbachia pipiensis (Hertig et Wolbach, 1924) is known to manipulate the exprebion of genes implicated in the metabolism, immunity and reproduction in Drosophila melanogaster (Meigen, 1830). Under streb, cells activate the cellular streb response (CSR). The CSR is a conserved network of pathways regulating identification, check and response to streb, preserving the cellular homeostasis. The CSR involves the unfolded protein response, autophagy, the heat shock response and other subcellular pathways. How Wolbachia affects the CSR has not yet been investigated. Here, we report the influence of Wolbachia infection and cold streb on the exprebion of the Heat-shock-protein-70Aa (Hsp70Aa), Autophagy-related gene-1 (Atg1) and X box binding protein-1 (Xbp1) genes and the influence of cold streb on the Wolbachia surface protein gene (wsp). The Hsp70Aa, Atg1, and Xbp1 genes were affected by Wolbachia infection since they were found to be up-regulated in the Wolbachia-free flies. After cold streb, the Wolbachia-infected flies showed high exprebion of the Atg1 and Hsp70Aa genes in comparison to the Wolbachia-free flies. Moreover, cold streb negatively influenced the exprebion of the wsp gene
Cross talk at the cytoskeleton-plasma membrane interface: impact on neuronal morphology and functions
No full textThe cytoskeleton and its associated proteins present at the plasma membrane not only determine the cell shape but also modulate important aspects of cell physiology such as intracellular transport including secretory and endocytic pathways. Continuous remodeling of the cell structure and intense communication with extracellular environment heavily depend on interactions between cytoskeletal elements and plasma membrane. This review focuses on the plasma membrane- cytoskeleton interface in neurons, with a special emphasis on the axon and nerve endings. We discuss the interaction between the cytoskeleton and membrane mainly in two emerging topics of neurobiology: (i) production and release of extracellular vesicles and (ii) local synthesis of new proteins at the synapses upon signaling cues. Both of these events contribute to synaptic plasticity. Our review provides new insights into the physiological and pathological significance of the cytoskeleton-membrane interface in the nervous system
DNA (CYTOSINE-5) METHYLTRANSFERASE TURNOVER AND CELLULAR LOCALIZATION IN DEVELOPING P. LIVIDUS SEA URCHIN EMBRYO.
No full textRNA interference machinery-mediated gene regulation in mouse adult neural stem cells
Full text linkBackground: Neurogenesis in the brain of adult mammals occurs throughout life in two locations: the subventricular zone of the lateral ventricle and the subgranular zone of the dentate gyrus in the hippocampus. RNA interference mechanisms have emerged as critical regulators of neuronal differentiation. However, to date, little is known about its function in adult neurogenesis. Results: Here we show that the RNA interference machinery regulates Doublecortin levels and is associated with chromatin in differentiating adult neural progenitors. Deletion of Dicer causes abnormal higher levels of Doublecortin. The microRNA pathway plays an important role in Doublecortin regulation. In particular miRNA-128 overexpression can reduce Doublecortin levels in differentiating adult neural progenitors. Conclusions: We conclude that the RNA interference components play an important role, even through chromatin association, in regulating neuron-specific gene expression programs
Characterization of a new variant DNA (cytosine-5)-methyltranferase unable to methylate double stranded DNA isolated from the marine annelid worm Chaetopterus variopedatus.
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