1,720,992 research outputs found
Molecular fingerprinting of Helicobacter pylori strains from duodenal ulcer patients.
No full textAims: To characterize the molecular fingerprinting of Helicobacter pylori population isolated in duodenal ulcer
patients treated with triple therapy.
Methods and Results: Gastric biopsy specimens from corpus and antrum, were cultured for H. pylori isolation.
Helicobacter pylori eradication was evaluated after 4 and 16 weeks. DNAs of all isolates were characterized by
random amplified polymorphic DNA typing and cagA gene was also detected. After the therapy, five patients
harboured the microorganism at 4 weeks and two of them remained H. pylori positive at 16 weeks. The analysis of
DNA fingerprinting of strains isolated from antrum and corpus of patients susceptible to treatment, showed similar
patterns. Instead, when the therapy was not effective, strains isolated from sequential biopsies from initial and after
4 and 16 weeks, showed distinct fingerprintings and retained the cagA status, over time.
Conclusions: The drugs used for therapy could exercise an effect in genotypical rearrangement among H. pylori
cells.
Significance and Impact of the Study: The variableness among H. pylori strains represents a way to challenge
environmental stress
Helicobacter pylori in fase planctonica e sessile: comportamento in risposta a diversi terreni di coltura
No full textDetection of free and plankton-associated Helicobacter pylori in seawater.
No full textAims: To detect both free and plankton-associated Helicobacter pylori in seawater samples collected on the Italian
coast of the Adriatic Sea using a nested-PCR.
Methods and Results: Dissolved oxygen, pH, salinity and chlorophyll a were the parameters recorded together
with the characterization of zooplanktonic organisms. Plankton-associated H. pylori DNA was searched for in water
samples filtered through 200 and 64 lm nylon nets whereas free bacteria were retained with the subsequent
filtration through 0Æ22 lm pore-size membranes. Nested-PCR using primers for the glmM (ureC) gene was
performed to reveal the presence of H. pylori. The DNA sequencing of amplified products confirmed the specificity
of the assay. The sensitivity of the nested-PCR assay for H. pylori detection was 62 CFU per 100 ml in spiked water
samples. Helicobacter pylori either free or bound to planktonic organisms was found in seven of 12 monthly samples.
In particular, free bacteria were detected during the summer sampling and in November, December and March
associated to planktonic cells.
Conclusions: The presence of free and plankton-associated H. pylori in seawater suggests that it can be a
significant reservoir and a potential route of transmission for the microorganism.
Significance and Impact of the Study: Our study seems to provide a promising background to define new and
effective strategies for surveillance of this human pathogen
Anti-Helicobacter pylori specific antibody immunohistochemistry improves the diagnostic accuracy of Helicobacter pylori in biopsy specimen from patients treated with triple therapy.Am. J. Gastroenterol
No full textOBJECTIVE: To investigate the effectiveness of immunohistochemical technique to detect Helicobacter pylori (H. pylori) in patients treated with triple therapy. METHODS: Forty patients (18 men, 22 women, mean age 43 years) with active antral gastritis, H. pylori positive at urease test, culture, and histology, were treated for 1 wk with omeprazole, amoxicillin, and metronidazole. Gastritis was scored according to Sydney criteria. Two months after the end of therapy, endoscopy, urease test, culture, and histology were repeated. RESULTS: Culture and histology were negative in 32 (80%) of treated cases. Biopsy specimens of the eradicated group were stained with immunohistochemical technique using an anti-H. pylori specific polyclonal antibody. In 12 of 32 (37.5%) patients, clusters of round or vibrio-shaped bacteria, unidentified at histology, were stained by the specific anti-H. pylori antibody. After triple therapy, at histology all patients were found with improved gastritis. In six patients however, mucosal-associated lymphoid tissue (MALT) appearance, present before therapy, persisted after therapy. In five of six patients with MALT, immunostaining with anti-H. pylori antibody was positive. CONCLUSIONS: The immunohistochemical technique is more accurate than classical methods in identifying H. pylori after specific therapy. This method should, therefore, be used in all studies that aim to achieve eradication. Whether the H. pylori identified at immunohistochemistry is able to reactivate and induce recrudescence of infection remains to be clarified
In vitro activity of Aloe vera inner gel against microorganism grown in planktonic and sessile phase
No full textAloe barbadensis Miller (Aloe vera) is a herbal remedy widely used for a variety of illnesses. A large number of biological activities have been ascribed to A.vera leaf extracts to explain its purported health benefits, including antimicrobial, anti-inflammatory, lipid and glucose lowering, antiproliferative, immunostimulatory, and antioxidant functions. In the first part of our study, antimicrobial activity of A.vera inner gel was determined against a panel of Gram positive and negative bacteria (Staphylococcus aureus ATCC 29213, S.epidermidis ATCC 35984, Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 9027) and Candida albicans ATCC 10231. In addiction, being A.vera inner gel used in the treatment of peptic ulcer and in the treatment of dermatological and wound healing, A.vera inner gel was tested on planktonic and sessile phase of clinical Helicobacter pylori strains (including multi-drug-resistant strains) and against S.aureus and P.aeruginosa clinical isolates from leg ulcer
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