1,720,967 research outputs found
Reconstituted extracellular matrix improve osteoblastic differentiation onto titanium surfaces
No full textElectrospinning technique is an efficient processing method to manufacture micro- and nano-sized fibrous structures by electrostatic force for different applications. In biomaterial field, electrospinning technique has been successfully utilized to prepare new drug delivery materials and tissue engineering scaffolds. Fiber mats of biodegradable polymers having a diameter in the nano- to submicro-scale can be considered to mimic the nanofibrous structure of native extracellular matrix (ECM).
Native extracellular matrix, constituted of proteins and polysaccharides improving cells growth in its nanofibrous porous structure, controls not only the cell phenotype, but the whole structure of the biological tissues.
In the present study we investigated the effect of electrospun reconstituted collagen fibers onto metals for oral implants devices manufacturing as far as the osteoblastic differentiation potential of stem cells and cytofunctionality of osteoblasts in-vitro. The cells cultured onto titanium samples coated with ECM constituents showed faster osteoblastic differentiation and more efficient deposition of mineralized matrix in comparison with those onto uncoated substrates
Effectiveness of topical mesalazine in the management of patients with oral lichen planus
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Ability of polyurethane foams to support cell proliferation and the differentiation of MSCs into osteoblasts
No full textIn bone tissue reconstruction, the use of engineered constructs created by mesenchymal stem cells (MSCs) that differentiate and proliferate into three-dimensional porous scaffolds is an appealing alternative to autologous and heterologous bone grafts. Scaffolds considered in this work are represented by polyurethane (PU) foams. Two PU foams (EG-1 and EC-2) were synthesized and characterized for morphology, mechanical properties and in vitro interaction with the osteoblast-like cell line MG63 and MSCs from human bone marrow. EC-1 and EC-2 showed similar densities (0.20 g cm(-3)) with different morphologies: EC-1 showed a more homogeneous pore size (average Phi = 691 mu m) and distribution, with a 35% open porosity, whereas EC-2 evidenced a wide range of pore dimension, with an average pore size of 955 mu m and a 74% open porosity. The compressive properties of the two foams were similar in the dry condition and both showed a strong decrease in the wet condition. In vitro tests showed good MG63 cell proliferation, as confirmed by the results of the MTT assay and scanning electron microscopy (SEM) observations, with a higher cell viability on EC-2 foam 7 days post-seeding. In the experiments with MSCs, SEM observations showed the presence of an inorganic phase deposition starting day 7 onto EC-1, day 14 onto EC-2. The inorganic particles (CaP) deposition was much more evident onto the pore surface of both foams at day 30, indicating good differentiation of MSCs into osteoblasts. Both PU foams therefore appeared to stimulate cell adhesion and proliferation in vitro, sustaining the MSCs' growth and differentiation into osteoblasts
Lichen planus and hepatitis C virus: a multicentre study of patients with oral lesions and a systematic review.
No full textAn association between hepatitis C virus (HCV) infection and lichen planus (LP) has been investigated, but results have been inconsistent.
OBJECTIVES:
To investigate the relationship between LP and HCV seropositivity. Methods In a cross-sectional study we tested the sera of 303 consecutive newly diagnosed patients with histologically proven LP referred to three Italian centres for the presence of anti-HCV IgG. A comparable control group was also tested. Next, in a systematic review, studies were identified by searching different databases in April 2004. Inclusion criteria were: (i) analytical study design; (ii) clinical and histological diagnosis of LP; and (iii) serological test for anti-HCV antibodies as main outcome. The risk of bias was assessed on the basis of characteristics of the study group, appropriateness of the control group and study design. Pooled data were analysed by calculating odds ratios (ORs), using a random effects model.
RESULTS:
In the cross-sectional study, nearly one in five (19.1%) of the LP group was HCV positive, while a much lower prevalence of infection was found in the control group (3.2%) [OR 7.08; 95% confidence interval (CI) 3.43-14.58]. The systematic review yielded 25 relevant studies, six of which had a low risk of bias. There was a statistically significant difference in the proportion of HCV-seropositive subjects among patients with LP, compared with controls (OR 4.80; 95% CI 3.25-7.09). Following subgroup analyses, the variability of HCV prevalence in patients with LP seemed to depend on geographical area, but not on age.
CONCLUSIONS:
Anti-HCV circulating antibodies are more common in patients with LP than in controls, although such an association may not be significant in some geographical areas
Accuracy of referrals to a specialist oral medicine unit by general medical and dental practitioners and the educational implications
No full textThe effect of levosulpiride in burning mouth syndrome
No full textAIM: This paper evaluated the efficacy of the systemic administration of levosulpiride, an antipsychotic and antidepressant drug, in the management of burning mouth syndrome.
METHODS: Forty-four subjects suffering from idiopathic burning mouth syndrome were enrolled in the study. Patients underwent a thorough clinical examination, including salivary flow rate measurements, laboratory tests (complete blood cell counts, blood glucose levels, serum iron and transferrin levels, serum vitamin B(12), and folate levels), patch tests for contact allergy to denture materials, and isolation of Candida species from oral mucosal scrapes. Oral symptoms before and after treatment were assessed using a visual analogue scale. All the patients used systemic levosulpiride (100 mg/daily) for 8 weeks.
RESULTS: Of the 39 patients who completed the protocol, none reported complete remission of the oral burning sensation. However, 28 patients reported at least some improvement, and these patients had had oral symptoms for significantly less time than those who reported no improvement.
CONCLUSIONS: These preliminary data suggest that levosulpiride is more effective in patients who have recently developed burning/stinging oral sensation
Ability to support cell proliferation and to stimulate MSCs differentiation of polyurethane foams
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