1,721,032 research outputs found
Quantitative analysis of caffeic acid phenethyl ester in crude propolis by liquid chromatography-electrospray ionization mass spectrometry.
No full textCaffeic acid phenethyl ester (CAPE), an active component of propolis from honeybee hives, is known to have antimitogenic, anticarcinogenic, antinflammatory, and immunomodulatory properties. The paper describes a rapid and simple liquid chromatography-electrospray ionisation mass spectrometry method for qualitative and quantitative determination of CAPE. The chromatographic separation was performed with a Luna RP-C18 column using a water-acetonitrile linear gradient. The method was linear over a 0.125-80 ng/mL range (LOD = 62.5 pg/mL). The method was applied for the quantitation of caffeic acid phenethyl ester in crude propolis samples, which were analysed directly after extraction with ethyl acetate solution
AFFINITY AND SPECIFICITY OF ANTI-GM1 ANTIBODIES IN THE EXPERIMENTAL AXONAL MODEL OF GUILLAIN-BARRE SYNDROME
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Integration of metabolomics and proteomics in multiple sclerosis: from biomarkers discovery to personalized medicine
No full textPersonalized medicine is the science of individualized prevention and therapy. In the last decade, advances in high-throughput approaches allowed the development of proteomic and metabolomic studies in evaluating the association of genetic and phenotypic variability with Q2 disease sensitivity and analgesic response. These considerations have more value in case of multiple sclerosis (MuS), a multifactorial disease with high heterogeneity in clinical course and treatment response. In this review, we reported and updated about proteomic and metabolomic studies for the research of new candidate biomarkers in MuS, and difficulties in their clinical applications. We focused especially on the description of both “omics” approaches that, once integrated, may synergically describe pathophysiology conditions. To prove this assumption, we rebuilt interaction between proteins and metabolites described in the literature as potential biomarkers for MuS, and a pathway analysis of these molecules was performed. The result of
such speculation demonstrated a strong convergence of proteomic and metabolomic results in this field, showing also a poorness of available tools for incorporating “omics” approaches. In conclusion, the integration of Metabolomics and Proteomics may allow a more complete characterization of such a heterogeneous disease, providing further insights into personalized healthcare
Improving protein identification from linear MALDI-TOF spectra: validation of an automated tool for signal denoising and peak identification
No full textLipidomics analysis reveals low levels of phosphatidylcholines and sphingomyelins in cerebrospinal fluido f multiple sclerosis patients.
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Visible Photostability of Ruthenium and Platinum Phthalocyanines in Water and in the presence of Organic Substrates
No full textStereoselective double reduction of 3-methyl-2-cyclohexenone by use of palladium and platinum nanoparticles in tandem with alcohol dehydrogenase
Full text linkThe combination of metal nanoparticles (Pd or Pt NPs) with NAD-dependent thermostable alcohol dehydrogenase (TADH) resulted in the one-flask catalytic double reduction of 3-methyl-2-cyclohexenone to 3-(1S,3S)-methylcyclohexanol. In this article some assumptions about the interactions between a chemocatalyst and a biocatalyst have been proposed. It was demonstrated that the size of the NPs was the critical parameter for the mutual inhibition: the bigger the NPs the more harmful for the enzyme they were even if the NPs themselves were only moderately inactivated. Conversely the smaller the NPs the more minimal the TADH denaturation although they were dramatically inhibited. Resuming the chemocatalysts were very sensitive to deactivation which was not related to the amount of enzyme used while the inhibition of the biocatalyst can be strongly reduced by minimizing the NPs/TADH ratio used to catalyze the reaction. Among some methods to avoid direct binding of NPs with TADH we found that using large Pd NPs and protecting their surfaces with a silica shell the overall yield of 3-(1S,3S)-methylcyclohexanol was maximized (36%).BT/Biocatalysi
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