1,721,031 research outputs found
Mutagenicity of pesticides evaluated by means of gene conversion in Saccharomyces cerevisiae and in Aspergiullus nidulans.
No full textEvoluzione microbica durante la fase termofila di compostaggio in reattore orizzontale chiuso
No full textSewage sludge and agricolture waste hygienization through aerobic stabilization and composting.
No full text
Recovery of Salmonella typhimurium from compost with the IMS-PCR method
No full textIn this work a procedure was optimized to recover Salmonella typhimurium from a vegetable compost after its artificial contamination. A combination of a bacteriological method, immunomagnetic separation (IMS) and polymerase chain reaction (PCR) techniques allowed a reduction in the detection time to 30 h while maintaining high specificity. The lower threshold of direct amplification from extracts of contaminated compost was 10(8) salmonellae g(-1). To improve the sensitivity, a combination of shortened pre enrichment and enrichment procedures was optimized and the growth of S. typhimurium evaluated. Immunomagnetic separation using anti-Salmonella Dynabeads(TM) permitted the recovery of 30 salmonellae per 50 g of compost, the same value obtained with the traditional microbial method, which takes two days longer. Alternative purification methods to reduce organic compound inhibition of the PCR reaction mixture did not improve Salmonella detection under a threshold of 10(5) salmonellae g(-1)
Effects of Benomyl anf Captan on rhizosphere fungi and the growth of Allium cepa.
No full textThe effects of soil treatment with Benomyl or Captan on the rhizosphere fungi of Allium cepa were investigated. The mycoflora of the rhizosphere was considerably inhibited by both fungicides. Captan was more inhibiting than Benomyl, but the latter seemed to be more persistent. The fungi isolated from the rhizosphere of control plants, plants treated with Benomyl or with Captan are listed and differences between treatments are discussed. The total number of species isolated from the rhizosphere of control plants was 65, Benomyl 44 and Captan 29. The growth of onions receiving fungicide treatment was greatly reduced: differences in dry weight were 31% (Captan) and 36% (Benomyl) less than control plants
- …
