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Untitled Speech by Judge Ronald N. Davies, UND Commencement, June 4, 1961
No full textText of untitled speech delivered by Judge Ronald N. Davies at the UND Commencement on June 4, 1961. Judge Davies was a United States District Judge in North Dakota, as well as an UND alumni
Fergusobia rosettae Davies, n. sp.
No full textDescription of Fergusobia rosettae Davies n. sp. (Figs 1, 3 A, 4 A) = Fergusobia MSp 45 apud Davies et al., 2012 a Measurements. Table 2. Material examined. Holotype, parthenogenetic ♀, roadside at Yellow Waterhole, ~ 2 km north of Kennedy in coastal eastern QLD (18 º 11.30 ’S, 145 º 56.66 ’E). From basal shoot bud (‘rosette’) galls on Melaleuca quinquenervia (Cav.) S.T. Blake 1958, collected by K.A. Davies, 10.viii. 2006 and 18.viii. 2007. On slide with a paratype ♂, deposited at the ANIC, Canberra, ACT, Australia. Paratypes, WINC, The University of Adelaide, SA, Australia, 3 parthenogenetic ♀s, 1 pre-parasitic infective ♀, 10 ♂ s, slide numbers WNC 2480; Queensland Museum, Brisbane, QLD, Australia, 10 parthenogenetic ♀s, 2 pre-parasitic infective ♀s, 12 ♂ s; and at the USDA Nematode Collection, Beltsville, MD, USA 1 parthenogenetic ♀ and 1 ♂. 15 parthenogenetic ♀♀, 4 pre-parasitic infective ♀♀, and 24 ♂♂, collected from the above location on 10.viii. 2006 and 18.viii. 2007. Description. Parthenogenetic female. Body shape arcuate when heat relaxed; relatively small (<0.3mm long); relatively broad compared to length; larger than amphimictic pre-parasitic females and smaller than males; body narrows behind vulva to form a short conoid tail. With light microscope, cuticle appears smooth, and longitudinal striae not apparent in sub-cuticle. Lateral fields not seen. Cephalic region 5–7 µm wide, ~ 80 % diameter of body at anterior end, off-set, 1 – 2 µm high, unstriated; lateral view with rounded outline and circum-oral area distinctly raised ~ 1 µm in most specimens. Stylet with cone ~ 40 % of length; basal knobs just higher than wide, ~ 2 µm wide at base, rounded. Orifice of dorsal pharyngeal gland ~ 1 µm posterior to stylet knobs. Anterior fusiform part of digestive tract occupying 53–74 % of body diameter, length 3 times diameter of tract. Lumen of tract broadens at distal end of dorsal pharyngeal gland. Pharyngeal glands extending over intestine, large, diameter ~ 47–75 % of body diameter, distance from head to end of glands being 45–66 % (mean 55 %) of total body length. Gland nucleus prominent, with obvious nucleolus. Secretory/excretory pore with obscure duct, opens posterior to nucleus of pharyngeal gland; secretory/ excretory cell ovoid. Hemizonid 4 or 5 annules in front of secretory/excretory pore. Reproductive tract variable in length, extending to secretory/excretory pore, part-way along pharyngeal gland, to base of gland or not reaching it; flexed in 1 / 17 specimens examined; oviduct usually with two oocytes per row; quadricolumella not smooth, uterus not seen containing eggs, apparently not extensile; vulva a simple transverse slit with protruding lips in some specimens; no vulval plate. Anus pore-like. Tail relatively short, conoid; length 1–1.5 times anal body diameter; bluntly rounded tip. Infective pre-parasitic female. Infects mature larval stage of Fergusonina sp. or pupa. Arcuate to open-C shape when relaxed by heat; relatively slender; maximum body diameter at mid-body length; body tapers gradually behind vulva. Cuticle obscurely annulated, appears smooth; longitudinal striae not apparent with light microscope; lateral fields not seen. Cephalic region barely offset, domed shape; circum-oral area rounded; stylet slender, weakly sclerotised with tiny basal knobs ~ 1.5 µm wide; cone ~ 40 % of length. Orifice of dorsal pharyngeal gland not seen. Anterior fusiform part of digestive tract little expanded, occupying 63–66 % of body diameter, length 3.3–3.5 times diameter. Pharyngeal glands extending over intestine, diameter 54 (36–66)% of body diameter, extending over intestine, distance from head to end of glands being 34 (29–48)% of total body length. Secretory/excretory pore opens behind pharyngeal glands or opposite gland nucleus; duct obscure; secretory/ excretory cell not seen. Hemizonid not seen. Uterus ~ 70 % of total gonad length in uninseminated females, packed with sperm in inseminated females; vagina at right angle to body axis; reproductive tract extending to nerve ring; length of tract hypertrophied in some specimens. Vulva a transverse slit, vulval lips raised ~ 1 µm, no vulval plate present. Anus an obscure pore. Tail sub-cylindroid; relatively short; length 1–1.5 times diameter at anus, tip almost hemispherical. Male. Body almost straight to arcuate when relaxed by heat, tail region slightly curved ventrally. Cuticle obscurely annulated, longitudinal striae of sub-cuticle apparent when viewed with light microscope; lateral fields not seen. Cephalic region 5–7 µm wide, occupying 75–80 % anterior body diameter, offset, ~ 1.5–2 µm high; circum-oral area flat or raised, with lightly sclerotised framework; stylet with cone ~ 40 % of length, round stylet knobs 2–3 µm wide. Orifice of dorsal pharyngeal gland ~ 2 µm behind knobs. Anterior fusiform part of digestive tract occupying 52–83 % of body diameter, length 2–3 times diameter. Pharyngeal glands extending over intestine, diameter 46–80 % (mean 63 %) of body diameter, distance from head to end of glands being 32–59 % (mean 47 %) of total body length. Lumen of intestinal tract broadens behind pharyngeal gland. Secretory/excretory pore opens opposite nucleus of pharyngeal gland; duct obscure; secretory/excretory cell ovoid, ~ 5 µm long. Hemizonid extending over two annules, 4 or 5 annules in front of secretory/excretory pore. Reproductive tract with single testis, variable in length, usually extending to distal end of dorsal pharyngeal gland, barely overlapping it in three specimens, not flexed; testis, seminal vesicle and vas deferens not clearly differentiated. Bursa apparently leptoderan, smooth; may be prominent or obscure; arises 40 – 50 % along length of body from tail tip, terminates just in front of tail tip. Spicules paired, broadly angular to arcuate, bent at about 40 % of length, with manubrium and shaft longer than blade; moderately sclerotised; manubrium wider than shaft, may be off-set on dorsal edge; blade narrows gradually to bluntly rounded tip with concavity on distal edge; opening sub-terminal. Inconspicuous muscles associated with cloaca. Tail arcuate, slightly ventrally concave, conoid; length 1.5–2.5 times diameter at cloaca; bluntly to broadly rounded tip. Diagnosis and relationships. Fergusobia rosettae n. sp. is morphologically characterized by the combination of a small, arcuate parthenogenetic female having a short conoid tail with a bluntly rounded tip, an arcuate, relatively slender, infective female with an almost hemispherical tail tip, and an arcuate male with an arcuate to angular (not heavily sclerotised) spicule and leptoderan bursa arising at 40–50 % of body length. Morphologically, Fergusobia rosettae n. sp. is similar to F. pohutukawa Davies 2007 (in Taylor et al. 2007), F. jambophila Siddiqi 1986, F. tolgaensis n. sp., F. cajuputiae Davies & Giblin-Davis 2004, F. nervosae Davies & Giblin-Davis 2004, and F. sporangae Davies 2013 (in Davies et al. 2014 a). From phylogenetic analyses based on sequences of D 2 /D 3, large sequence divergences support F. rosett ae n. sp. as a unique species. It is genetically close to an undescribed species (Fergusobia sp. 1 in Davies & Giblin-Davis 2004) from a similar gall form on M. nervosa (Ye et al. 2007). The parthenogenetic female of F. ro s et t a e n. sp. (arcuate shape) differs from F. brevicauda Siddiqi 1994, F. brittenae Davies 2010 (in Taylor & Davies 2010), F. cosmophyllae Davies 2013 (in Davies et al. 2013 b), F. diversifoliae Davies 2013 (in Davies et al. 2013 b), F. fasciculosae Davies 2012 (in Davies et al. 2012 b), F. floribundae Davies 2013 (in Davies et al. 2013 b), F. gomphocephalae Davies 2013 (in Davies et al. 2014 c), F. indica Jairajpuri 1962 sensu Siddiqi 1986, F. leucoxylonae Davies 2014 (in Davies et al. 2014 c), F. magna Siddiqi 1986 sensu Davies 2010 (in Davies et al. 2010 b), F. microcarpae Davies 2014 (in Davies et al. 2014 b), F. minimus Lisnawita 2013 (in Davies et al. 2013 b), F. morrisae Davies 2012 (in Davies et al. 2012 b), F. pimpamensis Davies 2013 (in Davies et al. 2013 b), F. planchonianae Davies 2014 (in Davies et al. 2014 b), F. porosae Davies 2013 (in Davies et al. 2013 a), F. ptychocarpae Davies 2008 (in Taylor & Davies 2008), F. tumifaciens (Currie 1937) Wachek 1955 sensu Davies 2014 (in Davies et al. 2014 b), F. v i m i n al i s a e Davies 2014 (in Davies et al. 2014 b), and F. viridiflorae Davies & Giblin-Davis 2004 (C-shape). In length (228–269 µm), it is shorter than the parthenogenetic female of F. rileyi Davies 2012 (in Davies et al. 2011 a) (310–394 µm). In having cuticle which does not swell upon fixation; it differs from F. jambophila Siddiqi 1986 and F. pohutukawa Davies 2007 (in Taylor et al. 2007) in which it does. Having a distinctly raised circum-oral area separates the parthenogenetic female of F. rosettae n. sp. from those of F. cajuputiae, F. colbrani Davies 2014 (in Davies et al. 2014 a), F. delegatensae, F. fisheri Davies & Lloyd 1996, F. leucadendrae Davies & Giblin-Davis 2004, and F. tumifaciens, in which it is flat or only slightly raised. The stylet (8–10 µm) of this female is longer than in F. curriei Fisher & Nickle 1968 (5–8 µm) and F. juliae Davies 2012 (in Davies et al. 2012 b) (5–7 µm); and shorter than in F. camaldulensae Davies 2012 (in Davies et al. 2012 a) (11–13 µm), F. schmidti Davies & Bartholomaeus 2014 (in Davies et al. 2014 c) (11–14 µm), and F. tumifaciens (19 µm). In having an enormous oesophageal gland (b’ 1.5–2.2), it is similar to F. quinquenerviae Davies & Giblin-Davis 2004 but lacks the extra lobe or flex found in the gland of the latter. In F. rosettae n. sp., the vulva (V 83 –93%) is more posterior than in F. nervosae Davies & Giblin-Davis 2004 (81–83 %). In having a body behind the vulva that narrows gradually, is arcuate and conoid in shape, with a broadly rounded tip, this female differs from that of F. dealbatae Davies & Giblin-Davis 2004, F. eugenioidae Davies 2012 (in Davies et al. 2012 b), and F. philippinensis Siddiqi 1994 (more slender, arcuate to straight). In length (9–21 µm, mean 14 µm), the tail of the F. rosett ae n. sp. parthenogenetic female is usually shorter than in F. tolgaensis n. sp. (mean 22 µm, range 18–25 µm). This female lacks the broad opening of the stylet aperture present in F. sporangae Davies 2014 (in Davies et al. 2014 c). It is morphologically close to but can be separated from the undescribed species of Fergusobia (Species 1) from ‘rosette’ galls on M. nervosa in having the hemizonid at 4–5 vs 6–8 annules in front of the excretory pore (Davies & Giblin-Davis 2004). The infective female of F. ros e t t a e n. sp. (open C-shape) differs in shape from that of F. brevicauda, F. camaldulensae, F. colbrani, F. curriei, F. delegatensae, F. diversifoliae, F. fisheri, F. leucadendrae, F. leucoxylonae, F. microcarpae, F. quinquenerviae, and F. viridiflorae (arcuate to barely J), and from F. rileyi (almost straight). In length (250–267 µm), it is smaller than the female of F. brittenae (375–550 µm), F. c ol b r an i (369–405 µm), F. cosmophyllae (374–448 µm), F. dealbatae (307–347 µm), F. eugenioidae (438 µm), F. fasciculosae (268–332 µm), F. floribundae (357–450 µm), F. juliae (396–550 µm), F. magna (537–633 µm), F. morrisae (322–395 µm), F. pimpamensis (369–443 µm), F. philippinensis (290–370 µm), F. planchonianae (303–339 µm), F. porosae (277–300 µm), F. ptychocarpae (387–471 µm), F. sporangae (289–353 µm), and F. viminalisae (334–437 µm). The stylet (7–8 µm) of F. rosettae n. sp. is longer than in F. minimus (4–6 µm). It has a subcylindroid tail with a tip that is almost hemisperical, separating it from F. tolgaensis n. sp., in which the tail tip is broadly rounded. It is difficult to morphologically separate the infective female of F. rosett ae n. sp. and F. cajuputiae, although the former tends to be slimmer (respective diameters 22–23 µm and 27–30 µm). Body diameter also separates F. rosettae n. sp. and F. gomphocephalae and F. schmidti (respectively, diameters 22–23 µm vs 26–32 µm and 30–48 µm; ratio a 10.8–11.5 vs 8.6–10.7 and 6.5–10.8). Given the small sample size for the infective female of F. nervosae (n= 1), and their morphological similarity to F. rosettae n. sp., it is not possible to separate them. In shape (arcuate), the heat-relaxed male of F. ro s e t t ae n. sp. differs from those of F. brittenae, F. curriei, and F. fasciculosae (J-shape), F. pimpamensis (J or C-shape), F. diversifoliae, F. juliae, F. magna, F. planchonianae, F. ptychocarpae, and F. viridiflorae (with strongly curved posterior). In length (246–319 µm), it is smaller than the male of F. brevicauda (330–420 µm), F. camaldulensae (383–451 µm), F. delegatensae (350–518 µm), F. diversifoliae (413–459 µm), F. eugenioidae (341–420 µm), F. floribundae (403–570 µm), F. morrisae (347–413 µm), F. pohutukawa (398–469 µm), and F. r i l e yi (378–508 µm). The ratio a (8.5–11.4) is smaller than in males of F. pohutukawa (12.2–15.5). In length (8–9 µm), the stylet is shorter than in F. leucoxylonae (10–13 µm) and tends to be shorter than that of F. tumifaciens (8.5–10 µm). The shape of the tail (barely arcuate with a broadly rounded tip) differs from that of F. philippinensis (truncate tip) and from F. leucadendrae (bluntly rounded tip). Spicule length (14–17 µm) is shorter than in F. colbrani (17–21 µm), F. dealbatae (18–22 µm), F. juliae (20–27 µm), F. quinquenerviae (16–20 µm), F. schmidti (19–25 µm), and F. sporangae (17–25 µm). The bursa arises at ~ 40–50 % of body length from the tail tip, differing from F. cosmophyllae, F. fisheri, F. minimus, F. porosae, and F. tumifaciens in which it is shorter (respectively, 12–39, ~ 20, 12–28, 15– 33, and less than 20 %) and from F. cajuputiae and F. jambophila in which it is longer (more than 50 %). In having a raised circum-oral area, this male is separated from those of F. microcarpae and F. nervosae, in which it is flat or less raised. The shape of the spicule differs in F. rosettae n. sp. and F. gomphocephalae and F. viminalisae, being more angular in the latter two species. In size, shape, length of bursa, form of cephalic area and of stylet, the male of F. rosettae n. sp. and F. tolgaensis n. sp. are similar. However, they can be separated on the position of the hemizonid (4–5 vs 2 annules in front of the excretory pore), and the spicule is more slender in F. tolgaensis n. sp. Etymology. Named after the form (resembling a rosette) of the gall from which the nematodes were collected.Published as part of Davies, Kerrie A., Ye, Weimin, Giblin-Davis, Robin M., Taylor, Gary S., Purcell, Matthew & Thomas, Kelley, 2014, Nematodes from galls on Myrtaceae. IX. Fergusobia rosettae n. sp. on Melaleuca quinquenervia and F. tolgaensis n. sp. on Syzygium luehmannii, from Queensland, pp. 214-236 in Zootaxa 3889 (2) on pages 216-220, DOI: 10.11646/zootaxa.3889.2.3, http://zenodo.org/record/22749
Fergusobia colbrani Davies, n. sp.
No full textFergusobia colbrani Davies n. sp. (Figs 1 E, 2 E, 3 E, 4) Measurements. Table 3. Material examined. The description presented here is based on measurements of 26 parthenogenetic ♀s, 2 pre-parasitic infective ♀s, and 26 ♂ s; from a tree of Angophora floribunda (Sm.) Sweet 1830 growing naturally in Paradise Park at Murrurundi, NSW, Australia (31 º 76 ’S, 150 º 83 ’ E.); associated with an undescribed species of Fergusonina (MSp 49 in Davies et al. 20011 a; WINC nos 0 0 3222, 063857). Extracted from spheroid leaf galls on A. floribunda. Coll. K.A. Davies, 16.iv. 2001 and 6.v. 2008. Holotype: a parthenogenetic female, together with an infective female and a male, on a slide deposited in the ANIC, Canberra, ACT, Australia, collected at Murrurundi, NSW, data as above. Vouchers (collection data as above) deposited at the WINC, The University of Adelaide, SA, Australia, 5 parthenogenetic ♀s, 5 ♂ s; Australian National Museum, Sydney, NSW, Australia, 15 parthenogenetic ♀s, 1 preparasitic infective ♀, 15 ♂ s; and at the USDA Nematode Collection, Beltsville, MD, USA 5 parthenogenetic ♀s and 5 ♂ s. subvelutina Colbran, 1967 Yarraman Description. Parthenogenetic female. From soft spheroid galls on leaves on A. floribunda, containing one or two locules. Body open C-shape to sub-spiral, dorsally curved with ventral side convex; smaller than amphimictic pre-parasitic females and males; body narrows gradually behind vulva to form conoid tail with broadly rounded tip. With light microscope, cuticle appears smooth to weakly annulated, sub-cuticle with longitudinal striae. Lateral fields not seen. Cephalic region ca 80 % diameter of body at anterior end, off-set, ca 2 µm high, unstriated; rounded outline in lateral view, circum-oral area slightly raised. Stylet small with cone 40–50 % length, basal knobs rounded, just higher than wide, ca 2 µm wide at base. Orifice of dorsal pharyngeal gland ca 1 µm posterior to stylet knobs. Anterior fusiform part of digestive tract occupying 51–79 % (mean 65 %, n = 6) body diameter, 1.9–2.8 × diameter; lumen of tract broadens partway along dorsal pharyngeal gland. Pharyngeal glands huge, diameter 35–80 % (average 62 %, n = 19) of body diameter, extending over intestine, distance from head to end of glands being 45 (37–55) % of total body length. Secretory/excretory pore opens opposite nucleus of pharyngeal gland; duct obscure, secretory/excretory cell not seen. Hemizonid not seen. Reproductive tract variable in length, extending to base of pharyngeal gland, part-way along gland or to nerve ring; with 1 to 4 flexures; oviduct with oocytes in pairs for the first few rows, subsequently no apparent pattern of arrangement; quadricolumella not smooth, uterus not extensile, with no eggs in 5 specimens, and 1 in 21 specimens examined; vulva a simple transverse slit with non-protruding rounded lips; no vulval plate. Anus pore-like, in some specimens opens into a small dimple in the cuticle. Tail conoid, short, length 0.5–1.5 × anal body diameter; broadly rounded tip. Infective pre-parasitic female. From spheroid galls on leaves on A. floribunda. Infects mature larval stage of Fergusonina sp. or pupa. Arcuate shape when heat-relaxed; maximum body diameter at mid-body length; body tapers gradually behind vulva. Cuticle obscurely annulated, longitudinal striae of sub-cuticle not apparent with light microscope; lateral fields not seen. Cephalic region just offset, rounded shape; circum-oral area rounded, flat or slightly raised; stylet slender, with rounded basal knobs higher than wide, ca 1 µm in diameter; cone ca 40 – 50 % of length. Orifice of dorsal pharyngeal gland obscure, ca 1 µm posterior to stylet knobs. Anterior fusiform part of digestive tract little expanded, occupying 38–63 % of body diameter, length 1.6–3.3 × daimeter (n = 2). Pharyngeal glands extending over intestine, diameter 55–75 % body diameter, distance from head to end of glands is average 27.5 % of body length. Secretory/excretory pore opens opposite nucleus of pharyngeal gland; duct obscure; secretory/excretory cell not seen. Hemizonid immediately anterior to secretory/excretory pore. Uterus ca 60 % of total gonad length in uninseminated females, packed with sperm in inseminated females; vagina anteriorly directed; reproductive tract extending to level of excretory/secretory pore. Vulva a transverse slit, vulval lips not raised, rounded, no vulval plate present. Anus an obscure pore. Tail sub-cylindroid; relatively short (c = 17–20); length approximates body diameter at anus; tip almost hemispherical. Male. From spheroid galls on leaves on A. floribunda. Body arcuate to barely J-shaped when heat-relaxed, tail region slightly curved ventrally. Cuticle obscurely annulated, longitudinal striae of sub-cuticle apparent with light microscope; lateral fields not seen. Cephalic region 6.5 –8.0 µm wide, occupying ca 70–80 % anterior body diameter, offset, ca 2 µm high; circumoral area not raised, with lightly sclerotised framework; stylet with cone 40–50 % of length, round stylet knobs ca 2 µm wide. Diameter of anterior fusiform part of digestive tract 51–86 % of body diameter, length 1.4–2.8 × diameter. Orifice of dorsal pharyngeal gland ca 1 µm behind knobs. Pharyngeal glands extending over intestine, diameter 41 (29–53) % of body diameter (n = 16), length from head to end of glands 31 (26–47) % of total body length. Secretory/excretory pore opens opposite or slightly posterior to nucleus of pharyngeal gland; duct obscure, not heavily sclerotised; secretory/excretory cell not seen. Hemizonid immediately anterior to duct, extending over 2 annules. Testis single, variable in length, usually overlaps dorsal pharyngeal gland and extends to nerve ring but may only reach base of gland; reflexed in a few specimens; cap cell often offset, seminal vesicle and vas deferens not clearly differentiated. Bursa apparently leptoderan, reaching to tail tip but not enveloping it, smooth; may be prominent or obscure; arises 30 – 65 % of length of body anterior to tail tip. Spicules paired, angular at about 40 % of length, with manubrium and shaft longer than blade; moderately sclerotised; manubrium wider than shaft, not offset; blade having similar diameter to shaft, tip notched, opening terminal. Inconspicuous muscles associated with cloaca. Tail conoid, arcuate, ventrally concave; length 1.5 –2.0× diameter at cloaca; bluntly rounded tip. Diagnosis and relationships. Fergusobia colbrani n. sp. is morphologically characterized by the combination of a small C-shaped parthenogenetic female with a short broadly conoid tail, an arcuate infective female with an almost hemispherical tail tip, and an arcuate to barely J-shaped male with an angular spicule having a notched tip and mid-length leptoderan bursa. Its species status is supported by its development in an unusual spheroid leaf gall found only on host plants of the genus Angophora, and its association with a fergusoninid fly larva having a dorsal shield of the two ‘patches’ form. Morphologically, Fergusobia colbrani n. sp. is close to F. microcarpae Davies 2013 (in Davies et al. 2013 a), F. fisheri Davies & Lloyd 1996, MSp 11 from E. viminalis, and the Fergusobia spp. from the broad-leaved melaleucas. Molecular sequences are unavailable for phylogenetic analyses. The parthenogenetic female of F. col br ani n. sp. (C-shape to spiral) differs from F. fasciculosae Davies 2012 (in Davies et al. 2012 b) (arcuate); and from F. rileyi Davies 2012 (in Davies et al. 2012 a) (almost straight to arcuate). Its length (225–361 um) is shorter than that of F. magna Siddiqi 1986 sensu Davies 2010 (in Davies et al. 2010 b) (500–780 µm) and F. i nd i c a (Jairajpuri 1962) Siddiqi 1986 (525–626 µm). The stylet (7–9 µm) of this parthenogenetic female is longer than in F. curriei Fisher & Nickle 1968 (5–8 µm), F. juliae Davies 2013 (in Davies et al. 2013 b) (5–7 µm) and F. microcarpae (9.5–11 µm); and shorter than in F. camaldulensae Davies 2012 (in Davies et al. 2012 a) (11–13 µm) and F. pohutukawa Davies 2007 (in Davies et al. 2007) (10 µm). Its stylet is smaller than that of F. tumifaciens Currie 1937 (19 µm). In having a large to enormous pharyngeal gland (b’ 1.8–2.7), F. colbrani n. sp. is similar to F. quinquenerviae Davies & Giblin-Davis 2004 and F. viridiflorae Davies & Giblin-Davis 2004, but lacks the extra lobes or flexures. In having a body behind the vulva that narrows gradually, is arcuate and conoid in shape, with a broadly rounded tip, the female of F. col br ani n. sp. differs from F. cosmophyllae Davies 2011 (in Davies et al. 2013 b) (narrowly rounded tail tip); F. dealbatae Davies & Giblin- Davis 2004, F. delegatensae Davies 2013 (in Davies et al. 2013 b), F. eugenioidae Davies 2012 (in Davies et al. 2012 b), F. floribundae Davies 2013 (in Davies et al. 2013 b), F. philippinensis Siddiqi 1994, and F. ptychocarpae Davies 2008 (in Taylor & Davies 2008) (more slender tails); from F. po ro s ae Davies 2013 (in Davies et al. 2013 a) (tail with smaller volume); and from F. diversifoliae Davies 2013 (in Davies et al. 2013 b), F. morrisae Davies 2012 (in Davies et al. 2012 b), and F. pimpamensis Davies 2013 (in Davies et al. 2013 b) (bodies narrow rapidly behind the vulva). In having a non-extensile uterus, it is separated from F. brevicauda Siddiqi 1994, F. jambophila Siddiqi 1986 and F. rileyi, in which the uterus is extensile. It lacks the broad duct of the excretory/secretory system that is present in F. brittenae Davies 2010 (in Taylor & Davies 2010) and F. minimus Lisnawita 2013 (in Davies et al. 2013 b). It is similar to F. fisheri, but lacks the wrinkled cuticle on the ventral side. It is also similar to F. nervosae Davies & Giblin-Davis 2004, but tends to have a shorter tail relative to the anal body diameter (respectively, c ’ 0.6–1.4 vs 1.4 –2.0). Morphologically, the parthenogenetic female of F. colbrani n. sp. cannot be separated from that of F. cajuputiae Davies & Giblin-Davis 2004 and F. leucadendrae Davies & Giblin-Davis 2004, but they do differ biologically, developing on genetically divergent hosts in Angophora and Melaleuca (Davies et al. 2010 a). The infective female of F. colbrani n. sp. (arcuate) differs in shape from F. brittenae, F. diversifoliae, and F. fasciculosae (open C-shape); from F. floribundae, F. juliae, F. morrisae, F. ptychocarpae and F. viminalisae (strongly curved in posterior region), and from F. porosae and F. rileyi (almost straight). In length (369–405 µm), it is smaller than the infective female of F. curriei (417–489 µm), F. magna (537–633 µm) and F. minimus (419–458 µm); and larger than that of F. c a j u pu t i a e (239–309 µm), F. dealbatae (307–347 µm), F. leucadendrae (270–291 µm), and F. quinquenerviae (259–325 µm). The a ratio (12.7–13.1) is larger than in F. brevicauda (8.9–10.6), F. cosmophyllae (4.0– 6.8), and F. microcarpae (10.2–11.8). The infective female of F. c o l b r a ni n. sp. can be separated from that of F. camaldulensae and F. eugenioidae by having a flat, rather than a raised, circum-oral area. Its head capsule is distinctly offset and raised about 2 µm from the rest of the body, separating it from F. delegatensae, in which the head is not so clearly offset or raised. Fergusobia colbrani n. sp. has a broad tail with a sub-hemispherical tip, separating it from F. philippinensis, which has a tip that is truncate in shape. The infective female of F. pimpamensis, with c ’ ratio of 1.5–2.4, has a tail that is longer relative to its diameter than in F. colbrani n. sp. (0.8–1.2). Morphologically, the infective female cannot be separated from that of F. f i s h e r i. While only one infective female of F. nervosae has been examined, it appears to have a longer tail compared to body length than that of F. colbrani n. sp. (respectively, c 12 vs 17.3–20.5, and c ’ 1.6 vs 0.8–1.2). Similarly, F. viridiflorae (n = 1) appears to have a more anterior vulva (V% 79 vs 85–86) than in F. c o l b r a ni n. sp., but more specimens should be examined to confirm this. In having spicules with a notched tip, the male of F. colbrani n. sp. is separated from all other described fergusobiid males, in which the tip is not notched. The notch is apparently an indent in the cuticle at the point where the channel between the spicules, for passage of the sperm, opens. In addition, the spicule of F. colbrani n. sp. appears to be more heavily sclerotised than in the other species of Fergusobia collected from Angophora. Form of gall. Warty, spongy, spheroid galls on leaf blades (collected from Murrurundi district, NSW, Australia) (Fig. 7 F; and see Fig. 3 in Colbran (1964) for a similar gall form), protruding from only one surface of leaf. About 0.5 to 1 cm in diameter. Lack a clear epidermal covering, contain 1 to 3 locules. Morphology of dorsal shield. Mature third stage larvae of associated Fergusonina fly with a dorsal shield comprising two separate patches of heavily sclerotised cuticle (Fig. 8). The first is on TS 3, and has a posterior patch of moderately sclerotised cuticle confluent with the second heavily sclerotised patch on AS 1, with a weakly sclerotised patch posterior to it. The whole shield is surrounded by sparse sclerotised raised spicules. Etymology. Named after the late Dr. Bob Colbran, of Brisbane, Queensland, plant and soil nematologist, who first photographed and recorded spheroid leaf galls on Angophora. Fergusobia Morphospecies A (WNC no. 2214) (Figs 1 C; 2 C; 3 C, 5, Table 2); associated with an undescribed species of Fergusonina (WINC no. 003225). Form of gall. ‘Leafy’ leaf bud galls (collected from Murrurundi district, NSW, Australia). Similar to, but smaller than, those described from E. viminalis and E. bridgesiana (Taylor et al. 2005); about 0.5 cm in diameter. Galls form within 2 or more fused leaf blades, bounded by developing leaves of the bud which usually grow past the galled tissue. Multilocular. Morphology of nematodes. Parthenogenetic female of medium size, relatively slender; arcuate to open Cshape; large pharyngeal gland; uterus non-extensile; V about 85 %; slender conoid tail. Infective female not collected. Male medium to large; slender, usually C-shape but may be arcuate; medium pharyngeal gland; spicule arcuate to angular with manubrium and shaft longer than blade, manubrium wider than shaft; relatively slender arcuate tail with slightly expanded tip; bursa arises at 70–90 % of body length anterior from tail tip, from near excretory/secretory pore to near anterior end of body. Morphology of dorsal shield. Shield absent. Fergusobia Morphospecies B (MSp 50 in Davies et al. (2010 a), WNC nos 2013, 2077, 2215, 2235) (Figs 1 D; 2 D,F; 3 D,F; 6; Table 2); associated with an undescribed species of Fergusonina (WINC no. 003224). Form of gall. Multilocular axillary vegetative bud ‘stem’ galls (various collections from Breeza, Wiseman’s Ferry and Murrurundi districts, NSW, Australia) (Fig. 7 B–E). Typically rounded to elliptical, with irregular shape and diameter 0.5 –2.0 cm. They are amorphous in form and variable in volume, depending on the number and arrangement of locules, usually 10. Morphology of nematodes. Parthenogenetic female relatively mid-size, slender; C-shape; large pharyngeal gland; uterus non-extensile, V about 80 %; slender arcuate conoid tail. Infective female arcuate shape with a hemispherical tail tip. Male medium to large; slender, arcuate or J-shape; medium pharyngeal gland; angular spicule with manubrium and shaft longer than blade, manubrium wider than shaft but not offset; slender tail; bursa arises at about 25–50 % of body length anterior to tail tip. Morphology of dorsal shield. Shield absent.Published as part of Davies, Kerrie A., Taylor, Gary S., Nelson, Leigh A., Yeates, David & Giblin-Davis, Robin M., 2014, Nematodes from galls on Myrtaceae. VI. Fergusobia from galls on Angophora in Australia, with description of F. colbrani n. sp. and key, pp. 326-348 in Zootaxa 3856 (3) on pages 328-342, DOI: 10.11646/zootaxa.3856.3.2, http://zenodo.org/record/22534
Fergusobia gomphocephalae Davies, n. sp.
No full textDescription of <i>Fergusobia gomphocephalae</i> Davies n. sp. <p>(Figs 2, 3, 7 I, 8J)</p> <p> = <i>Fergusobia</i> MSp. 9 <i>apud</i> Davies <i>et al</i>. 2010 <b>a</b></p> <p> <b>Measurements.</b> Table 2.</p> <p> <b>Material examined.</b> The description presented here is based on measurements of 21 parthenogenetic ♀s, 9 pre-parasitic infective ♀s, and 16 ♂ s. From Cervantes Caravan Park, Cervantes, 250 km north of Perth, WA, Australia (30°31´S 115°05´E), and Tuart Reserve, Cervantes, WA, Australia (30°25´S 115°08´E). Taken from unilocular bud galls on <i>Eucalyptus gomphocephala</i> associated with <i>Fergusonina newmani</i> Tonnoir 1937. Collected by K.A. Davies, 24.x.2000.</p> <p> <b>Holotype.</b> Parthenogenetic female on a slide deposited in the ANIC, Canberra, ACT, Australia, collected at Cervantes Caravan Park, Cervantes, Western Australia, together with one infective female and one male; collection data as above.</p> <p> <b>Paratypes.</b> Nine parthenogenetic ♀s, 3 pre-parasitic infective ♀s, 6 ♂ s were deposited at the WINC, The University of Adelaide, SA, Australia, 10 parthenogenetic ♀s, 5 pre-parasitic infective ♀s, 8 ♂ s at Western Australian Museum, Perth, WA, Australia, and 1 parthenogenetic ♀, 1 pre-parasitic infective ♀, and 1 ♂ at the USDA Nematode Collection, Beltsville, MD, USA. Collection data as above.</p> <p>Holotype Parthenogenetic females Males Infective females</p> <p>Partheno-genetic</p> <p>female</p> <p> <b>Description.</b> Parthenogenetic female. From unilocular ‘pea’ galls on young leaves and stems on <i>E. gomphocephala</i>. When heat relaxed, body usually dorsally curved with ventral side convex to form a C-shape, with greatest curvature behind the vulva; body narrows sharply behind vulva to form conoid tail, with most narrowing on ventral side; similar in size to pre-parasitic infective female and to male. With light microscope, cuticle has obscure annuli, and longitudinal striae are apparently present. Using SEM, cuticle is clearly annulated (~ 1µm wide), no longitudinal striae. While not seen with the light microscope, with SEM lateral fields arise ~20 µm behind anterior end, ~2 µm wide at mid-body, contain many clear, diagonal striae; which change at level of vulva to become less defined and irregular and continue almost to tail tip.</p> <p>Cephalic region 70% diameter of body at anterior end, offset, 1–2 µm high, unstriated; in lateral view has rounded outline and circum-oral area flat or slightly raised. With SEM, 4 distinct, respectively alternating with 4 indistinct, sectors are apparent. Six small cephalic papillae can be seen on the circum-oral area, surrounding the stylet opening. Amphid openings rounded, pore-like and closer to the edge of the sectors than the circum-oral area. Stylet 7–9 µm long, with conus 40–50% of length, basal knobs 1–2 µm wide at base, small, round.</p> <p>Orifice of dorsal pharyngeal gland 1–2 µm posterior to stylet knobs. Anterior fusiform part of digestive tract occupying 53–73% of body diameter, length 1.8–2.5 times diameter; lumen of tract broadening behind dorsal pharyngeal gland. Pharyngeal glands extending over intestine, large, diameter 61–78% of body diameter, distance from head to end of glands being 53 (31–67)% of total body length.</p> <p>Secretory/excretory pore opens 72–93 µm from anterior end with prominent duct opening onto area of slightly raised cuticle, at about 20% length of pharyngeal gland; secretory/excretory cell not seen. Hemizonid extending over one annulus, 1 annulus anterior to secretory/excretory pore.</p> <p> Reproductive tract comprising ovary with growth zone occupying <i>~</i> 60% of length, oviduct comprising two or three rows of cells with structure unclear, no spermatheca, quadricolumella present, not smooth, uterine sac about one vulval body width in length. Tract variable in length, extending part-way along dorsal pharyngeal gland; not flexed but with cap cell usually offset from growth zone; latter with one or two oocytes per row; uterine sac containing no eggs or one (in 2 of 22 specimens examined) egg; vulva with protruding lips in some specimens, flat or a depressed slit in others. Tail conoid, may be concave on dorsal side, length 1–2 times anal body diameter, with a rounded tip.</p> <p> Infective pre-parasitic female. From unilocular ‘pea’ galls on young leaves and stems on <i>E. gomphocephala.</i> Penetrates mature larval stage of <i>Fn. newmani</i> or pupa. Body J or C-shaped when relaxed by heat, with greatest curvature behind vulva; maximum body diameter at mid-body in some and at vulva in others; cuticle with obscure annulations and longitudinal striae; lateral fields not seen.</p> <p>Cephalic region barely or not offset; circum-oral area flat or slightly raised; stylet slender, 6–10 µm long, weakly sclerotised with round basal knobsbeing slightly higher than wide, 1–2 µm in width; conus 50% of total stylet length.</p> <p>Orifice of dorsal pharyngeal gland barely posterior to stylet knobs. Anterior fusiform part of digestive tract occupying 48–64% of body diameter, 2.4–3.5 times diameter in length. Pharyngeal glands extending over intestine, 31–50% of body diameter in diameter, distance from head to end of glands being 30 (28–37)% of total body length.</p> <p>Secretory/excretory pore opens 59–83 µm behind anterior end, opposite pharyngeal gland nucleus; prominent duct opens onto area of slightly raised cuticle; secretory/excretory cell not seen. Hemizonid extending over one annule, 1 annule anterior to secretory/excretory pore.</p> <p>Uterus 80% of total gonad length in uninseminated female, packed with amoeboid sperms in inseminated female; vagina at right angle to body axis, plugged with refractive material; reproductive tract extending alongside dorsal pharyngeal gland; hypertrophy of tract in some specimens. Vulval lips flat or barely raised. Tail short, broad, in length 0.6–1.3 times diameter at anus, tip almost hemispherical and may be notched.</p> <p> Male. From unilocular ‘pea’ galls on young leaves and stems on <i>E. gomphocephala</i>. Body arcuate to J-shaped when relaxed by heat, tail region more or less curved ventrally. Cuticle with obscure annuli ~1 µm wide, longitudinal striations not seen; lateral fields not seen.</p> <p> Cephalic region offset, 1–2 µm high; circum-oral area flat or raised, with lightly sclerotised framework; stylet 7–10 µm long, with conus 50% of length, round stylet knobs with <i>~</i> 2 µm width. Anterior fusiform part of digestive tract occupying 51–75% of body diameter, 2.6–3.2 times diameter in length. Pharyngeal glands extending over intestine, occupying 50–70% of body diameter, distance from head to end of glands being 33–59% (mean 48%) of total body length; gland nucleus may be positioned at anterior or posterior end of gland. Lumen of intestinal tract broadens at posterior end or behind pharyngeal glands.</p> <p> Secretory/excretory pore opens 59–93 µm behind anterior end, at <i>~</i> 30–40% of length of pharyngeal gland and in front of gland nucleus; prominent duct opens onto area of slightly raised cuticle in some specimens; secretory/ excretory cell not seen. Hemizonid extending over two annuli, barely anterior to secretory/excretory pore.</p> <p> Reproductive tract with single testis, variable in length, may extend to base of pharyngeal glands, or to secretory/excretory pore, but usually overlaps dorsal pharyngeal gland; may be reflexed; testis, seminal vesicle and <i>vas deferens</i> not clearly differentiated. Bursa membranous, smooth or crenate, peloderan; may be prominent or obscure; arises 30 <b>–</b> 50% along length of body. Spicules paired, angular near their middle, small but robust; moderately sclerotised; manubrium offset in some specimens, similar to or slightly wider than shaft; blade narrows gradually with notch on proximal side similar to the tip; opening terminal. Inconspicuous muscles associated with cloaca. Tail ventrally concave, curved; in length 1.1–2.6 times diameter at cloaca, tip bluntly rounded.</p> <p> <b>Molecular phylogenetic relationships.</b> For molecular analysis, the D2/D3 expansion segments of LSU (AY589352) and mtCOI (AY589435) of V63 (<i>F. gomphocephalae</i> <b>n. sp</b>.) were sequenced. Based on the combined data from D2/D3 and mtCOI, V63 is in a monophyletic clade with V4 (<i>F. juliae</i> from FBG on <i>E. macrorrhyncha</i>), V32 (MSp 28 from <i>Eucalyptus</i> sp.), V65 (<i>F. fasciculosae</i> from stylet galls on <i>E. fasciculosa</i>) and V71 (<i>F. leucoxylonae</i> <b>n. sp</b>.) (Ye <i>et al.</i> 2007, Fig. 5). The blastn search of LSU (853 bp sequenced) on V63 revealed it has 6–12-bp differences (99% identity) and 2–4 gaps with V32 (AY589328), V65 (AY589354), V68 (AY589357), V71 (AY589360) and V314 (FJ386989). The blastn search of mtCOI (618 bp sequenced) on V63 revealed it has 37–45- bp differences (93–94% identity) with V4 (AY589406), V65 (AY589437), V69 (AY589440) and V741 (EF 011098) (<i>F. leucoxylonae</i> <b>n. sp</b>.). These large sequence divergences support <i>F. gomphocephalae</i> <b>n. sp.</b> as a unique species.</p> <p> <b>Diagnosis and relationships.</b> <i>Fergusobia gomphocephalae</i> <b>n. sp.</b> (MSp 9 in Davies <i>et al.</i> 2010a) is morphologically characterized by the combination of a small C-shaped parthenogenetic female with a variable, conoid tail, a small C-shaped infective female with a hemispherical tail tip, and an arcuate or J-shaped male with broad tail, angular spicule and short peloderan bursa. Its status as a species is also supported by a combination of molecular analyses, gall form, association with <i>Fergusonina (Fn.) newmani</i>, host <i>E. gomphocephala</i>, and geographic distribution (collected only within the natural range of the host plant in WA).</p> <p> All stages are morphologically similar to those of <i>F. leucoxylonae</i> <b>n. sp.</b>, <i>F. sporangae</i> <b>n. sp.</b>, <i>F. microcarpae</i> Davies 2013 (in Davies <i>et al.</i> 2013a) and <i>F. porosae</i> Davies 2013 (in Davies <i>et al.</i> 2013a). In addition, parthenogenetic and infective females are similar to those of <i>F. fisheri</i> Davies & Lloyd 1996.</p> <p> From phylogenetic analyses based on sequences of D2/D3 and mtCOI, <i>F. gomphocephalae</i> <b>n. sp.</b> is genetically close to <i>Fergusobia</i> spp. from pea galls and flower bud galls on <i>E. microcarpa</i>, <i>F. fasciculosae</i> Davies 2012 (in Davies <i>et al.</i> 2012b) from stylet galls on <i>E. fasciculosa</i>, and <i>F. juliae</i> Davies 2012 (in Davies <i>et al.</i> 2012b) from flower bud galls on <i>E. macrorhyncha</i>.</p> <p> In body shape (C-shaped), the parthenogenetic female of <i>F. gomphocephalae</i> <b>n. sp.</b> differs from <i>F. camaldulensae</i> Davies 2012 (in Davies <i>et al.</i> 2012a) and <i>F. sporangae</i> <b>n. sp.</b> (arcuate); and <i>F. rileyi</i> Davies 2012 (in Davies <i>et al.</i> 2012a) (almost straight). In body length (239–331 µm), <i>F. gomphocephalae</i> <b>n. sp.</b> is shorter than <i>F. brittenae</i> (328–461 µm) Davies 2010 (in Taylor & Davies 2010), <i>F. cosmophyllae</i> Davies 2013 (in Davies <i>et al.</i> 2013b) (354–406 µm), <i>F. delegatensae</i> Davies 2013 (in Davies <i>et al.</i> 2013b) (345–527 µm), <i>F. eugenioidae</i> Davies 2012 (in Davies <i>et al.</i> 2012b) (298–354 µm), <i>F. magna</i> Siddiqi 1986 <i>sensu</i> Davies 2010 (in Davies <i>et al</i>. 2010b) (500–780 µm), and <i>F. i n di ca</i> (Jairajpuri 1962) Siddiqi 1986 (525–626 µm). The stylet (7–9 µm) of the parthenogenetic female is shorter than that in <i>F. camaldulensae</i> (11–13 µm), <i>F. indica</i> (12–15 µm), <i>F. microcarpae</i> (9.5–11 µm), <i>F. pohutukawa</i> Davies 2007 (in Taylor <i>et al.</i> 2007) (10–11 µm), <i>F. rileyi</i> (10–13 µm) and <i>F. sporangae</i> <b>n. sp.</b> (12–14 µm); and tends to be shorter than in <i>F. planchonianae</i> Davies 2013 (in Davies <i>et al.</i> 2013b) (9–12 µm), <i>F. tumifaciens</i> (Currie 1937) Wachek 1955 <i>sensu</i> Davies 2014 (in Davies <i>et al.</i> 2014b) (9–11 µm), and <i>F. viminalisae</i> Davies 2014 (in Davies <i>et al.</i> 2014b) (9–10 µm). In having large to enormous pharyngeal glands (<i>b’</i> 1.5–3.2), it differs from <i>F. jambophila</i> Siddiqi 1986, and <i>F. philippinensis</i> Siddiqi 1994 which have smaller glands; and from <i>F. quinquenerviae</i> Davies & Giblin-Davis 2004 which has glands that are reflexed or have an extra lobe. The shape (conoid, with tail with straight sides and bluntly rounded tip) of the body behind the vulva in <i>F. gomphocephalae</i> <b>n. sp.</b> differs from that of <i>F. dealbatae</i> Davies & Giblin-Davis 2004 (more slender); <i>F. diversifoliae</i> Davies 2013 (in Davies <i>et al.</i> 2013b) (narrows more rapidly); <i>F. curriei</i> Fisher & Nickle 1968, <i>F. fisheri</i> (narrows more gradually); <i>F. pohutukawa</i> (conoid, straight); <i>F. floribundae</i> Davies 2013 (in Davies <i>et al.</i> 2013b), <i>F. juliae,</i> <i>F. morrisae</i> Davies 2012 (in Davies <i>et al.</i> 2012b), <i>F. pimpamensis</i> Davies 2013 (in Davies <i>et al.</i> 2013b), <i>F. porosae</i>, <i>F. planchonianae</i> Davies 2014 (in Davies <i>et al.</i> 2014b) and <i>F. ptychocarpae</i> Davies 2008 (Taylor & Davies 2008) (more slender); <i>F. brevicauda</i> Siddiqi 1994, <i>F. cajuputiae</i> Davies & Giblin-Davis 2004, <i>F. camaldulensae</i>, <i>F. fasciculosae</i>, <i>F. leucadendrae</i> Davies & Giblin-Davis 2004, <i>F. nervosae</i> Davies & Giblin-Davis 2004 and <i>F. viridiflorae</i> Davies & Giblin-Davis 2004 (tips more broadly rounded). The parthenogenetic female of <i>F. gomphocephalae</i> <b>n. sp.</b> tends to have a smaller stylet than that of <i>F. leucoxylonae</i> <b>n. sp.</b> and <i>F. planchonianae</i> (respectively, 7–9 µm <i>vs</i> 9–11.5 µm and 9–12 µm). It is difficult to separate the parthenogenetic female of <i>F. gomphocephalae</i> <b>n. sp.</b> and <i>F. minimus</i> Lisnawita 2013 (in Davies <i>et al.</i> 2013b) but the latter tends to be larger, to be more tightly coiled when heat relaxed, and to have the body behind the vulva forming a more elongate conoid shape. Morphologically, the parthenogenetic female of <i>F. gomphocephalae</i> <b>n. sp.</b> cannot be separated from that of <i>F. schmidti</i> <b>n. sp.</b> and <i>F. colbrani</i> Davies 2014 (in Davies <i>et al.</i> 2014a).</p> <p> The infective female (body an open C-shape with strongly curved posterior region) of <i>F. gomphocephalae</i> <b>n.</b></p> <p> <b>sp.</b> differs in shape from those of <i>F. cajuputiae, F. camaldulensae</i>, <i>F. colbrani</i>, <i>F. cosmophyllae, F. dealbatae, F. delegatensisae, F. diversifoliae</i>, <i>F. fasciculosae</i>, <i>F. floribundae</i>, <i>F. leucadendrae, F. leucoxylonae</i>, <i>F. m a gn a, F. nervosae</i>, <i>F. pimpamensis</i>, <i>F. philippinensis, F. porosae, F. quinquenerviae,</i> and <i>F. viridiflorae</i> (arcuate to open C); from <i>F. eugenioidae</i>, <i>F. juliae</i>, <i>F. morrisae</i>, and <i>F ptychocarpae</i> (strongly curved in posterior region); and from <i>F. rileyi</i> (almost straight). In length (222–298 µm), the female is smaller than <i>F. magna</i> (537–633 µm), <i>F. brevicauda</i> (330–410 µm), <i>F. brittenae</i> (375–550 µm), <i>F. camaldulensae</i> (346–454 µm), <i>F. colbrani</i> (369–405 µm), <i>F. cosmophyllae</i> (374–448 µm), <i>F. curriei</i> (417–489 µm), <i>F. delegatensae</i> (375–452 µm), <i>F. diversifoliae</i> (357–473 µm), <i>F. eugenioidae</i> (438 µm), <i>F. juliae</i> (396–550 µm), <i>F. minimus</i> (419–458 µm), <i>F. morrisae</i> (322–395 µm), <i>F. pimpamensis</i> (369–443 µm), <i>F. planchonianae</i> (303–339 µm), <i>F. ptychocarpae</i> (387–471 µm), <i>F. rileyi</i> (378–432 µm), <i>F. tumifaciens</i> (345–445 µm), and <i>F. vim inal isae</i> (334–437 µm). The shape of the tail tip (hemispherical) differs from that of <i>F. microcarpae</i> (broadly rounded). The infective female of <i>F. gomphocephalae</i> <b>n. sp.</b> tends to be smaller than those of <i>F. f i s he r i</i> (mean 272 µm, 222–298 µm <i>vs</i> mean 349 µm, 241–395 µm). There is a tendency for the vulva to be more anterior in <i>F. gomphocephalae</i> <b>n. sp.</b> than in <i>F. sporangae</i> <b>n. sp.</b> (mean 77%, 73–81% <i>vs</i> mean 83%, 80–88%). Morphologically, the infective female of <i>F. gomphocephalae</i> <b>n. sp.</b> cannot be separated from that of <i>F. schmidti</i> <b>n. sp.</b></p> <p> In shape (arcuate to J-shaped), the male of <i>F. gomphocephalae</i> <b>n. sp.</b> differs from those of <i>F. jambophila</i> (almost straight). Length (228–283 µm) is shorter than in <i>F. magna</i> (446–588 µm), <i>F. brevicauda</i> (330–420 µm), <i>F. camaldulensae</i> (383–451 µm), <i>F. c o l b r a ni</i> (288–481 µm), <i>F. cosmophyllae</i> (374–502 µm), <i>F. curriei</i> (370–492 µm), <i>F. dealbatae</i> (314–432 µm), <i>F. delegatensae</i> (350–518 µm), <i>F. diversifoliae</i> (413–459 µm), <i>F. eugenioidae</i> (341–420 µm), <i>F. fisheri</i> (292–453 µm), <i>F. floribundae</i> (403–570 µm), <i>F. juliae</i> (377–453 µm), <i>F. minimus</i> (368–502 µm), <i>F. morrisae</i> (347–413 µm), <i>F. pimpamensis</i> (407–525 µm), <i>F. philippinensis</i> (280–390 µm), <i>F. pohutukawa</i> (398–469 µm), <i>F. planchonianae</i> (291–373 µm), <i>F. ptychocarpae</i> (405–535 µm), <i>F. r i l e yi</i> (378–508 µm), <i>F. sporangae</i> <b>n. sp.</b> (292–400 µm), <i>F. tumifaciens</i> (314–481 µm), <i>F. viminalisae</i
Davies, N S, NX27850
No full textThis record was harvested from a previous catalogue system and will be withdrawn in 2025. Information in this record may be superseded or incomplete. Visit this record in UMA's new catalogue at: https://archives.library.unimelb.edu.au/nodes/view/380761Surname: DAVIES
Given Name(s) or Initials: N S
Military Service Number or Last Known Location: NX27850
Missing, Wounded and Prisoner of War Enquiry Card Index Number: 34453195459
Item: [2016.0049.13054] "Davies, N S, NX27850
Fergusobia janetae Davies & Ye & Taylor & Scheffer & Bartholomaeus & Giblin-Davis 2018, n. sp.
No full textFergusobia janetae Davies n. sp. apud MSp 40 (Davies et al. 2012a) (Fig. 1) Measurements. Table 2. Material examined. Holotype: Parthenogenetic female, near Busselton, WA, Australia (33°38.62´S, 115°26.59´E). From roadside vegetation; unilocular galls on the blades of leaves of Eucalyptus marginata Donn ex Smith, collected J. Walker and K.A. Davies, 30.x.2000. On a slide with a paratype infective female and a male, deposited in the ANIC, Canberra, ACT, Australia. Paratypes: Vouchers (collection data as above) deposited at the WINC, The University of Adelaide, SA, Australia, 8 parthenogenetic ♀ s and 6 Ƌs on slides numbered WINC 004288-89 (WNC 2209); at the Western Australian Museum, Perth, WA, Australia, 8 parthenogenetic ♀ s and 8 Ƌs on slides; and at the USDA Nematode Collection, Beltsville, MD, USA 1 parthenogenetic ♀ and 1 Ƌ on a slide. Fifteen parthenogenetic ♀ s, one preparasitic infective ♀ and 18 ♂ s examined. Description. Parthenogenetic female. Body straight to arcuate, spindle-shaped; relatively broad compared to length; of similar size to males; body narrows gradually from about one body diameter anterior to vulva to form a straight conoid tail (Fig. 1A). With light microscope, cuticle appears smooth, and longitudinal striae in sub-cuticle are clear. Lateral fields with seven incisures (Fig. 1K). Cephalic region 6–9 µm in diameter, ~75–80% diameter of body at anterior end, off-set, 1.5 – 3 µm high, unstriated, not annulated; rounded outline in lateral view, circum-oral area slightly raised (Fig. 1B). Scanning electron microscopy shows that there are 6 rounded ‘lips’, with the two lateral lips being slightly narrower than the other four, and sub-triangular, with large openings for the amphids (Fig. 1B). Stylet sturdy, with cone usually less than 50% length, basal knobs as high as wide, ~ 2 µm wide at base, rounded. Orifice of dorsal pharyngeal gland ~ 1 µm posterior to stylet knobs. Anterior fusiform part of digestive tract not greatly expanded, occupying ~50–80% of body diameter; length 4.6 (3.7–6.0) times diameter (Fig. 1B). Valves apparently separating pharynx and intestine present 1–1.2 stylet lengths behind the stylet knobs, i.e. within the anterior fusiform part of digestive tract. Pharyngeal glands large, extending over intestine, occupying 57 (48–67)% of body diameter, distance from anterior end to end of glands being 20 (12–25)% of total body length. Intestinal lumen broadens from mid length to posterior of pharyngeal gland. Secretory/excretory pore opening posterior to pharyngeal gland; with non-refractile duct surrounded by prominent duct cell, secretory/excretory cell ellipsoid. Hemizonid positioned far anterior to pore, approximately at level of nucleus of dorsal gland cell. Reproductive tract variable in length, outstretched or flexed, extending part-way along dorsal pharyngeal gland or to nerve ring; oviduct with oocytes in pairs; uterus relatively long, extensile; about 30% of body length, containing 3 or more eggs; cells of quadricolumella prominent, smooth; vulva a simple slit. Anus a small pore. Tail conoid, straight, length 2–5 times anal body diameter, strongly annulated, narrowing gradually and equally to bluntly rounded tip (Figs 1A, G). Infective pre-parasitic female. The only specimen found was undergoing moult from J4 to adult stage (Fig. 1C). Infects mature larval stage or pupa of Fergusonina sp. Almost straight when relaxed by heat; maximum diameter at mid-body length, body narrows gradually behind vulva. Cuticle with inconspicuous annulations, clear longitudinal striae seen with light microscope; lateral fields not seen. Large nuclei present in body wall. Cephalic region ~ 90% diameter of body at anterior end, not offset, unstriated, ~ 8 µm in diameter, 2 µm high; circum-oral area flat. Stylet too weakly sclerotised to be observed. Orifice of dorsal pharyngeal gland not seen. Anterior part of digestive tract not expanded, occupying ~60% of body diameter, length 5 times diameter. Pharyngeal gland extending over intestine, occupying 40% body diameter, distance from anterior end to posterior end of glands being 22% body length. Secretory/excretory pore opening near posterior end of pharyngeal glands; hemizonid not seen. Uterus not fully developed, containing no sperm; vagina angled towards anterior end, about one vulval body diameter in length and occupying almost full body width, ovoid in shape, surrounded by apparent strong musculature; reproductive tract extending ~30% of distance between vulva and nerve ring. Vulval lips tiny, flat; no vulval plate apparent. Tail straight, length ~4 times body diameter at anus, tip narrowly rounded (Fig. 1C). Male. Body an open C-shape when relaxed by heat, tail region relatively slender (Fig. 1D). Cuticle appears smooth when viewed with light microscope; longitudinal striae clearly apparent in sub-cuticle; lateral fields faint, 5 or 6 lines present. Cephalic region 80–90% of anterior body diameter, offset, 6–8 µm in diameter, 2–4 µm high; circum-oral area flat or barely raised. Stylet sturdy, with cone ~40% of length, basal knobs spheroid, ~2 µm wide (Fig. 1H). Anterior fusiform part of digestive tract slender, occupying ~20–40% of body diameter; length 5.1 (4.1–5.9) times diameter; lumen broadening posterior to gland nucleus. Valves apparently separating pharynx and intestine, situated ~1 stylet length behind stylet knobs. Pharyngeal glands extending over intestine, 59 (50–68)% of body diameter, distance from anterior end to end of glands being 24 (20–34)% of total body length. Secretory/excretory pore opens posterior to pharyngeal gland; duct non-refractile, surrounded by prominent duct cell; excretory cell not seen. Hemizonid extending over two annules, positioned anterior to secretory/excretory pore in region level with or anterior to pharyngeal gland nucleus. Reproductive tract with single testis, extending to nerve ring; usually outstretched and occasionally flexed; testis, seminal vesicle and vas deferens of shorter tracts not clearly differentiated (Fig. 1D). Spermatids not in columns. Bursa smooth, prominent in most specimens; arises 95–98 (mean 96%) of length of body anterior to tail tip (Fig. 1H). The bursa widens just anterior to the cloaca; unclear if it terminates just anterior to the tail tip or surrounds it (Fig. 1D). Spicules paired, more or less angular near mid-length, relatively slender; relatively strongly sclerotised; in some specimens manubrium clearly offset on dorsal edge, wider than shaft; blade narrows gradually to bluntly rounded tip and may have convex curve on proximal edge (Fig. 1L). Large glands on vas deferens, associated with cloaca. Tail straight to ventrally arcuate, sub-conoid, ~3–5 times as long as diameter at cloaca, narrowing gradually to bluntly rounded or angular tip (Fig. 1J). Diagnosis and relationships. Fergusobia janetae n. sp. is morphologically characterized by the combination of a large, almost straight, spindle-shaped parthenogenetic female with an extensile uterus and a narrow conoid tail; an arcuate, relatively broad, infective female with a conoid tail having a narrowly rounded tip; and an open Cshaped male with an arcuate to angular spicule and bursa arising near the level of the stylet knobs. Morphologically, F. janetae n. sp. is most similar to F. magna Siddiqi 1986 sensu Davies 2010 (in Davies et al. 2010b), F. indica (Jairajpuri, 1962) Siddiqi, 1986 and F. rileyi, and also has similarities with F. linariifolia Davies 2014 (in Davies et al. 2014e) and F. pohutukawa Davies 2007 (in Taylor et al. 2007). Its status as a distinct species is corroborated by molecular data from sequencing of the relatively conserved 18S and 28S D2/D3 expansion segment. The parthenogenetic female of F. janetae n. sp. (a straight or arcuate spindle-shape when heat-fixed, with a long slender tail) differs from all described species of Fergusobia, except F. magna. Body length of the parthenogenetic female of F. janetae n. sp. overlaps with that of F. magna and F. indica (respectively, 514–723 vs 418–780 and 525–626 µm) and is larger than all other described species of Fergusobia. However, its straight to arcuate body separates female F. janetae n. sp. from F. magna which has a C-shaped parthenogenetic female. The presence of an extensile uterus in the parthenogenetic female of F. janetae n. sp. separates it from F. indica and also from F. cajuputiae Davies & Giblin-Davis, 2004, F. colbrani Davies 2014 (in Davies et al. 2014a), F. dealbatae Davies & Giblin-Davis, 2004, F. delegatensae Davies 2013 (in Davies et al. 2013b), F. eugenioidae Davies 2012 (in Davies et al. 2012b), F. fisheri Davies & Lloyd, 1996, F. leucadendrae Davies & Giblin-Davis, 2004, F. nervosa Davies & Giblin-Davis, 2004, F. philippinensis Siddiqi, 1994, F. schmidti Davies 2014 (in Davies et al. 2014c), F. quinquenerviae Davies & Giblin-Davis, 2004, F. rosettae Davies 2014 (in Davies et al. 2014d), F. rileyi Davies 2012 (in Davies et al. 2012a), F. sporangae Davies 2014 (in Davies et al. 2014d), F. tolgaensis Davies 2014 (in Davies et al. 2014d), F. tumifaciens (Currie 1937) Wachek 1955 sensu Davies 2014 (in Davies et al. 2014b), and F. decorae Davies 2014 (in Davies et al. 2014e), which lack extensile uteri. In having cuticle that does not swell upon fixation; it differs from F. linariifoliae and F. pohutukawa, and also from F. jambophila Siddiqi 1986, in which it does. Fergusobia janetae n. sp. is separated from F. rileyi by both body length and in having a longer tail (90 vs 40–50 µm). The only infective female of F. janetae n. sp. available for examination was moulting, and information about it is therefore limited. Its body length and stylet length are unlikely to accurately reflect that of mature individuals, i.e., cannot be used here as characters. Its arcuate shape differs from that of F. diversifoliae, F. fasciculosae Davies 2012 (in Davies et al. 2012b), F. gomphocephalae Davies 2014 (in Davies et al. 2014c), F. leucadendrae, F. nervosae, F. pimpamensis Davies 2013 (Davies et al. 2013b), F. philippinensis, F. rosettae, F. sporangae, F. tolgaensis Davies 2014 (in Davies et al. 2014c), and F. viminalisae Davies 2014 (in Davies et al. 2014b) (open Cshape), and from F. eugenioidae, F. juliae Davies 2012 (in Davies et al. 2012b), F. morrisae Davies 2012 (in Davies et al. 2012b), F. pruinosae n. sp., F. ptychocarpae Davies 2008 (in Taylor & Davies 2008), F. tumifaciens, and F. viminalisae (J-shaped). It has a straight slender sub-conoid tail with a bluntly rounded tip, separating it from F. brittenae Davies 2010 (in Taylor & Davies 2010), F. cajuputiae, F. curriei Fisher & Nickle 1968, F. leptospermum Davies 2017 (in Davies et al. 2017), F. leucadendrae, F. minimus Lisnawita (in Davies et al., 2013b), F. nervosae, F. pauciflorae n. sp., F. quinquenerviae, F. tolgaensis (with broadly rounded tips); F. eugenioidae, F. juliae, F. linariifoliae, F. morrisae, F. porosae Davies 2013 (in Davies et al. 2013a), F. pruinosae n. sp., F. ptychocarpae, F. tumifaciens, F. viminalisae, F. viridiflorae Davies & Giblin-Davis 2004 (with J–shaped tails); F. armillarisae Davies 2014 (in Davies et al. 2014), F. brevicauda Siddiqi 1994, F. camaldulensae, F. colbrani, F. cosmophyllae Davies 2013 (in Davies et al. 2013b), F. dealbatae, F. decorae, F. delegatensae, F. diversifoliae Davies 2013 (in Davies et al. 2013b), F. fasciculosae, F. fisheri, F. floribundae Davies 2013 (in Davies et al. 2013b), F. gomphocephalae, F. leucoxylonae Davies 2014 (in Davies et al. 2014c), F. microcarpae Davies 2013 (in Davies et al. 2013a), F. obliquae n. sp., F. pimpamensis, F. planchonianae Davies 2014 (in Davies et al. 2014b), F. robustae n. sp., F. rosettae, F. schmidti, F. sporangae (with sub-hemispherical tips); and from F. philippinensis (with a truncate tip). The infective female of F. janetae n. sp. has a more posterior vulva than that of F. magna (V% 65–72 vs 51–62). In length (639–750 µm), the male of F. janetae n. sp. is larger than all described species of Fergusobia. The shape of the tail (slender, arcuate with a bluntly rounded tip, long) is similar to that of F. magna and F. rileyi, but differs in being longer (82–111 vs 54–87 and 58–70 µm, respectively) and is slimmer than in F. magna (respectively, c’ ratio 3.3–4.8 vs 2.0–3.3). In length (9–11 µm), the stylet is similar to that of most described species, but longer than in F. minimus (4–7 µm). Spicule length (23–27 µm) is smaller than in F. magna (30–36 µm); but overlaps with or is longer than in other described species of Fergusobia. The shape of the spicules in F. janetae n. sp. (more or less angular) differs from those of F. jambophila, F. pimpamensis, F. rosettae, and F. decorae, in which it is clearly arcuate. In the male of F. janetae n. sp., the bursa extends over>90% of the body length, longer than in most Fergusobia spp., except for F. leptospermum (99%), F. linariifolia (90%), F. pohutukawa (99%), F. rileyi (90–95%) and F. viridiflorae (90%). Etymology. Named for the late Janet Walker, who directed the first author to the new species described here, in gratitude for her help with collecting, and happy memories of her zest for life, humour and patience.Published as part of Davies, Kerrie A., Ye, Weimin, Taylor, Gary S., Scheffer, Sonja, Bartholomaeus, F. & Giblin-Davis, Robin M., 2018, Nematodes from galls on Myrtaceae. XI. Descriptions of five new species of Fergusobia from Australia, pp. 1-31 in Zootaxa 4399 (1) on pages 3-7, DOI: 10.11646/zootaxa.4399.1.1, http://zenodo.org/record/120640
Davies, N, 2-Resd-1077
No full textThis record was harvested from a previous catalogue system and will be withdrawn in 2025. Information in this record may be superseded or incomplete. Visit this record in UMA's new catalogue at: https://archives.library.unimelb.edu.au/nodes/view/380737Surname: DAVIES
Given Name(s) or Initials: N
Military Service Number or Last Known Location: 2-RESD-1077
Missing, Wounded and Prisoner of War Enquiry Card Index Number: 18383(B)195435
Item: [2016.0049.13030] "Davies, N, 2-Resd-1077
Davies, K N B, 411293
No full textThis record was harvested from a previous catalogue system and will be withdrawn in 2025. Information in this record may be superseded or incomplete. Visit this record in UMA's new catalogue at: https://archives.library.unimelb.edu.au/nodes/view/380725Surname: DAVIES
Given Name(s) or Initials: K N B
Military Service Number or Last Known Location: 411293
Missing, Wounded and Prisoner of War Enquiry Card Index Number: 52444195423
Item: [2016.0049.13018] "Davies, K N B, 411293
Fergusobia armillarisae Davies, Giblin-Davis, Ye, Taylor, Makinson & Purcell, 2014, n. sp.
No full textFergusobia armillarisae n. sp. Davies (Figs 1, 4 A) = Fergusobia MSp 18 apud Davies et al. (2012 a) Measurements. Table 2. Material examined. The description presented here is based on measurements of 16 parthenogenetic ♀s, 2 infective ♀s and 19 ♂ s; Illalong Bay, Kuringai Chase, Sydney, NSW, Australia (33 ° 38.17´S 151 ° 13.32´E). Taken from unilocular pea galls on Melaleuca armillaris (Sol. ex Gaertn) Smith 1797. Collected by R.M. Giblin-Davis, K.A. Davies, Zeng Qi Zhao & Ian Riley, 4.vii. 2004. Holotype: One parthenogenetic female, together with an infective female and a male, on a slide deposited in the ANIC, Canberra, ACT, Australia; collection data as above. Paratypes: Vouchers (collection data as above) deposited at the WINC, The University of Adelaide, SA, Australia, 5 parthenogenetic females, 1 infective female and 8 males on slides numbered WINC 004345– 47 (WNC 2372); at the Australian National Museum, Sydney, NSW, Australia, 7 parthenogenetic females and 8 males on slides; and at the USDA Nematode Collection, Beltsville, MD, USA 3 parthenogenetic females and 2 males on slides. Description. Parthenogenetic female. Body shape variable, arcuate to open C-shaped, dorsally curved with ventral side convex and most curvature in tail region; relatively small; relatively broad; similar in size to amphimictic pre-parasitic females and smaller than males; body narrows behind vulva to form a conoid tail. With light microscope, cuticle appears smooth, sub-cuticle with strong longitudinal striae. Lateral fields not seen. Cephalic region ~ 70 % diameter of body at anterior end, offset, 2 µm high, unstriated; rounded outline in lateral view, circum-oral area flat or slightly raised. Amphids not seen. Stylet well sclerotised with cone occupying ~ 50 % of stylet length, basal knobs just higher than wide, 2–3 µm wide at base, rounded. Orifice of dorsal pharyngeal gland 1–2 µm posterior to stylet knobs. Anterior fusiform part of digestive tract occupying 38–66 % (mean 50 %, n = 11) of body diameter, length 2–3 times diameter; lumen of tract broadens at distal end of dorsal pharyngeal gland. Pharyngeal glands extending over intestine, occupying 50–76 % (mean 65 %) of body diameter, length from head to end of glands being 37 (30–50)% of total body length. Gland nucleus large, with medium-sized nucleolus. Holotype Partheno Parthenogenetic Males Infective females genetic female females Spicule length 18.4 ± 0.8 (17–19) Tail length 28 24 ± 3.2 45 ± 3.6 22 (18–32) (37–48) (21-23) Secretory/excretory pore opens anterior to nucleus of pharyngeal gland; secretory/excretory cell not seen. Hemizonid not seen. Reproductive tract variable in length, extending to secretory/excretory pore, part-way along pharyngeal gland or to nerve ring; flexed in 6 / 15 specimens examined; oviduct usually with two oocytes in a row; quadricolumella with clustered cells, uterus extensile, containing 1– 5 eggs; vulva a simple transverse slit with protruding rounded lips in some specimens; no vulval plate. Anus pore-like, opening in cuticular depression. Tail relatively short, conoid, concave on dorsal side; length 1.3–2.2 times anal body diameter; tip bluntly rounded. Infective pre-parasitic female. Arcuate shape when heat-relaxed; relatively broad; maximum body diameter at mid-body length; body tapers gradually in tail region. Cuticle obscurely annulated, less than 1 µm wide; longitudinal striae apparent with light microscope; lateral fields not seen. Cephalic region just offset, dome-shaped; circum-oral area rounded; stylet slender, weakly sclerotised with basal knobs being higher than wide; ~ 2 µm wide; cone ~ 40 % of stylet length. Orifice of dorsal pharyngeal gland 1–2 µm posterior to stylet knobs. Anterior fusiform part of digestive tract little expanded, occupying ~ 50 % of body diameter, length 2.0 – 2.5 times diameter. Pharyngeal glands occupying ~ 30 % of body diameter, extending over intestine, length from head to end of glands being ~ 30 % of total body length. Secretory/excretory pore opens behind pharyngeal glands; duct obscure; ellipsoid secretory/excretory cell ~ 7 µm long. Hemizonid not seen. Uterus ~ 70 % of total gonad length in uninseminated females, packed with sperm in inseminated females; vagina at right angle to body axis; reproductive tract extending to dorsal pharyngeal gland; hypertrophy of length of tract in some specimens. Vulva a transverse slit, vulval lips not raised, no vulval plate present. Anus an obscure pore. Tail relatively short, sub-cylindroid; length 1.3–1.5 times diameter at anus, tip broadly rounded. Male. Body arcuate when relaxed by heat, tail region slightly curved ventrally. Cuticle clearly annulated, annuli ~ 1 µm wide at mid-body length; strong longitudinal striae apparent with light microscope; lateral fields ~ 5 µm wide, lacking prominent lateral ridges, having broken diagonal striae. Cephalic region occupying ~ 70 %– 90 % anterior body diameter, offset, ~ 2 µm high; circum-oral area slightly raised, with lightly sclerotised framework; stylet well sclerotised with cone 30 % of length, stylet knobs higher than wide, 2–3 µm wide, rounded. Anterior fusiform part of digestive tract occupying 52–70 % of body diameter, length 2–3 times diameter. Orifice of dorsal pharyngeal gland ~ 2 µm behind knobs. Pharyngeal glands occupying 65 (47–82)% of body diameter, extending over intestine, length from head to end of glands being 33 (23–48)% of total body length. Lumen of intestinal tract broadens behind pharyngeal gland. Secretory/excretory pore opens opposite nucleus of pharyngeal gland; duct obscure; secretory/excretory cell ~ 5 µm long. Hemizonid lens-like, extending over two annules, 8 annules in front of secretory/excretory pore. Reproductive tract with single testis, variable in length, usually extending to nerve ring but may overlap dorsal pharyngeal gland; may be reflexed; testis, seminal vesicle and vas deferens not clearly differentiated. Bursa peloderan but may appear to be leptoderan, smooth; may be prominent or obscure; arises 50 % – 80 % along length of body. Spicules paired, angular at about half length, moderately sclerotised; with manubrium wider than shaft, may or may not be offset; blade narrows irregularly to bluntly rounded tip with concavity on distal edge; opening subterminal. Inconspicuous muscles associated with cloaca. Tail arcuate, ventrally concave, conoid; length 2.2–2.9 times diameter at cloaca; bluntly rounded tip. Molecular phylogenetic relationships. For molecular analysis, the partial SSU (V 410: FJ 393268), the D 2 / D 3 expansion segments of LSU (V 405: FJ 386994: V 410: FJ 386995) and mtCOI (V 405: FJ 386964) of F. armillarisae n. sp. were sequenced. Based on the LSU, V 405 and V 410 are close to each other and sister to V 412 (M. linariifolia n. sp.) (Davies et al., 2010 a, Fig. 78). A phylogenetic tree inferred from COI placed V 405 with V 462 (M. decora n. sp.) in a clade and in a basal position with many other Fergusobia species collected from Melaluca (Davies et al., 2010 a, Fig. 79). The blastn search of SSU of V 410 (1686 bp sequenced) revealed it has 7 to 11 -bp differences (99 % identity) from some Fergusobia species. The blastn search of LSU of V 410 (880 bp sequenced) revealed it has 9 -bp differences (94 % identity) and 3 gaps with V 405 and 40 -bp differences (95 % identity) and 16 gaps with V 412 (FJ 386996) (M. linariifolia n. sp.). The blastn search of COI of V 405 (618 bp sequenced) revealed the highest match was with V 462 (FJ 386982) (M. decora n. sp.) with 52 -bp differences (92 % identity). These large sequence divergences supported Fergusobia armillarisae n. sp. as a unique species. Diagnosis and relationships. Fergusobia armillarisae n. sp. is morphologically characterized by the combination of a medium-sized, arcuate parthenogenetic female with an extensile uterus, a narrow conoid tail; an arcuate, relatively broad, infective female with an almost hemispherical tail tip; and a small arcuate male with an angular spicule having an offset manubrium, and bursa arising at 50 %– 80 % of body length. Morphologically, F. armillarisae n. sp. is similar to F. linariifoliae n. sp., but lacks the swollen cuticle of the latter. The parthenogenetic female of F. armillarisae n. sp. (arcuate shape) differs from F. brevicauda Siddiqi, 1994, F. brittenae Davies, 2010 (in Taylor & Davies 2010), F. cosmophyllae Davies 2013 b (in Davies et al. 2013 b), F. diversifoliae Davies 2013 (in Davies et al. 2013 b), F. fasciculosae Davies 2012 (in Davies et al. 2012 b), F. floribundae 2013 (in Davies et al. 2013 b), F. gomphocephalae Davies 2014 (in Davies et al. 2014 c), F. indica (Jairajpuri, 1962) Siddiqi, 1986, F. leucoxylonae Davies 2014 (in Davies et al. 2014 c), F. magna Siddiqi 1986 sensu Davies 2010 (in Davies et al. 2010 b), F. microcarpae Davies 2013 (in Davies et al. 2013 a), F. minimus Lisnawita 2013 (in Davies et al. 2013 b), F. morrisae Davies 2012 (Davies et al. 2012 b), F. pimpamensis Davies 2013 (in Davies et al. 2013 b), F. planchonianae Davies 2014 (in Davies et al. 2014 b), F. porosae Davies 2013 (Davies et al. 2013 a), F. ptychocarpae Davies, 2008 (Taylor & Davies 2008), F. viminalisae Davies 2014 (in Davies et al. 2014 b), and F. viridiflorae Davies & Giblin-Davis, 2004 (C-shape). The presence of an extensile uterus in the parthenogenetic female of F. armillarisae n. sp. separates it from that of F. cajuputiae Davies & Giblin-Davis, 2004, F. colbrani Davies 2014 (in Davies et al. 2014 a), F. dealbatae Davies & Giblin-Davis, 2004, F. delegatensae Davies 2013 (in Davies et al. 2013 b), F. eugenioidae Davies 2012 (in Davies et al. 2012 b), F. fisheri Davies & Lloyd, 1996, F. leucadendrae Davies & Giblin-Davis, 2004, F. nervosa Davies & Giblin-Davis, 2004, F. philippinensis Siddiqi, 1994, F. schmidti Davies 2014 (in Davies et al. 2014 c), F. quinquenerviae Davies & Giblin-Davis, 2004, F. ro s et t a e Davies 2013 (in Davies et al. 2013 f), F. rileyi Davies 2011 (in Davies et al. 2011 a), F. sporangae Davies 2014 (in Davies et al. 2014 c), F. tolgarae Davies 2014 (in Davies et al. 2014 d), F. tumifaciens (Currie 1937) Wachek 1955 sensu Davies 2014 (in Davies et al. 2014 b), and F. decorae n. sp., which do not have extensile uteri. In having cuticle that does not swell upon fixation, it differs from F. jambophila Siddiqi 1986, F. linariifoliae n. sp. and F. pohutukawa Davies 2007 (in Taylor et al. 2007), in which it does. Having a flat or slightly raised circum-oral area separates the parthenogenetic female of F. armillarisae n. sp. and that of F. camaldulensae Davies 2011 (in Davies et al. 2011 a), in which it is distinctly raised. The stylet (9–12 µm) of this parthenogenetic female is longer than in F. curriei Fisher & Nickle 1968 (5–8 µm) and F. juliae Davies 2012 (in Davies et al. 2012 b) (5–7 µm). In having enormous oesophageal glands (b’ 1.5–2.2), it is similar to F. quinquenerviae but lacks the extra lobe or flex found in the gland of the latter. Infective females of F. armillarisae n. sp. have an arcuate shape, differing from that of F. diversifoliae, F. fasciculosae, F. gomphocephalae, F. leucadendrae, F. nervosae, F. pimpamensis, F. philippinensis, F. rosettae, F. sporangae, F. tolgarae, and F. viminalisae (open C-shape). With body length ranging from 279–291 µm, it is smaller than the female of F. brittenae (375–550 µm), F. colbrani (369–405 µm), F. cosmophyllae (374–448 µm), F. curriei (417–489 µm), F. dealbatae (307–347 µm), F. delegatensae (375–452 µm), F. eugenioidae (438 µm), F. floribundae (357–450 µm), F. juliae (396–550 µm), F. linariifoliae n. sp. (347–384 µm), F. magna (537–633 µm), F. microcarpae (302–341 µm), F. morrisae (322–395 µm), F. pimpamensis (369–443 µm), F. philippinensis (290–370 µm), F. planchonianae (303–339 µm), F. ptychocarpae (387–471 µm), F. sporangae (289–353 µm), and F. viminalisae (334–437 µm). In having a flat circum-oral area, the infective female of F. armillarisae n. sp. differs from those of F. camaldulensae, in which it is raised. It has a longer stylet than F. minimus and F. schmidti (respectively, 11–14 vs 4–5.5 and 5–10 µm). It has a short sub-cylindroid tail (21–23 µm) with an almost hemispherical tip, separating it from F. brevicauda (24–30 µm), F. d ec o r a e n. sp. (13–18 µm), F. f i s he r i (21–49 µm, mean 30 µm), F. leucoxylonae (11–18 µm), F. rileyi (40–50 µm with a bluntly rounded tip), and F. porosae and F. tolgarae in which the tip is bluntly rounded. Given that only two specimens of the infective female of F. armillarisae n. sp. were examined, it is difficult to separate it from the same stage collected from various broadleaved melaleucas. However, it appears to be shorter than F. viridiflorae (321 µm); and to have a more anterior secretory/excretory pore than F. cajuputiae and F. quinquenerviae (anterior end to secretory/excretory pore 47 µm vs 53–78 and 71–91 µm). In shape (arcuate), the male of F. armillarisae n. sp. differs from that of F. brittenae, F. curriei, and F. fasciculosae (J-shape), F. pimpamensis, F. schmidti (J or C-shape), F. magna, F. planchonianae, F. ptychocarpae, and F. viridiflorae (with strongly curved posterior). In length (302–400 µm), it is smaller than the male of F. diversifoliae (413–459 µm) and F. floribundae (403–570 µm). The male of F. armillarisae n. sp. has a flattened circum-oral area, but it is raised in F. camaldulensae, F. colbrani and F. jambophila. In length (9–12 µm), the stylet is shorter than in F. leucoxylonae (10–13 µm) and longer than in F. minimus (4–7 µm). The shape of the tail (arcuate with a bluntly rounded tip) differs from that of F. philippinensis (truncate tip). Spicule length (17–19 µm) is shorter than in F. juliae (20–27 µm). The shape of the spicules in F. armillarisae n. sp. (angular to arcuate) differs from those of F. brevicauda, F. cajuputiae, F. dealbatae, F. delegatensae, F. eugenioidae, F. gomphocephalae, F. leucadendrae, F. microcarpae, F. morrisae, F. pohutukawa, F. quinquenerviae, and F. viminalisae, in which it is clearly angular. In the male of F. armillarisae n. sp., the bursa arises at ~ 50 %– 80 % of the body length, separating it from those of F. cosmophyllae (~ 10 %– 40 %), F. decorae n. sp. (~ 30 %– 50 %), F. f i s h e r i (~ 20 %), F. linariifoliae n. sp. (80 %– 90 %), F. nervosae (~ 50 %), F. pohutukawa (~ 90 %), F. porosae (15 %– 33 %), F. rileyi (90 %– 95 %), F. rosettae (~ 40 %– 50 %), F. sporangae (10 %– 40 %) and F. tumifaciens (18 %– 22 %). Etymology. Named after M. armillaris, the plant species from which the nematodes were collected.Published as part of Davies, Kerrie A., Giblin-Davis, Robin M., Ye, Weimin, Taylor, Gary S., Makinson, Jeff & Purcell, Matthew, 2014, Nematodes from galls on Myrtaceae. X. Fergusobia from galls on narrow-leaved Melaleuca spp. in Australia, with descriptions of three new species, pp. 237-258 in Zootaxa 3889 (2) on pages 239-243, DOI: 10.11646/zootaxa.3889.2.4, http://zenodo.org/record/22465
Fergusobia armillarisae Davies, Giblin-Davis, Ye, Taylor, Makinson & Purcell, 2014, n. sp.
No full textFergusobia armillarisae n. sp. Davies (Figs 1, 4 A) = Fergusobia MSp 18 apud Davies et al. (2012 a) Measurements. Table 2. Material examined. The description presented here is based on measurements of 16 parthenogenetic ♀s, 2 infective ♀s and 19 ♂ s; Illalong Bay, Kuringai Chase, Sydney, NSW, Australia (33 ° 38.17´S 151 ° 13.32´E). Taken from unilocular pea galls on Melaleuca armillaris (Sol. ex Gaertn) Smith 1797. Collected by R.M. Giblin-Davis, K.A. Davies, Zeng Qi Zhao & Ian Riley, 4.vii. 2004. Holotype: One parthenogenetic female, together with an infective female and a male, on a slide deposited in the ANIC, Canberra, ACT, Australia; collection data as above. Paratypes: Vouchers (collection data as above) deposited at the WINC, The University of Adelaide, SA, Australia, 5 parthenogenetic females, 1 infective female and 8 males on slides numbered WINC 004345– 47 (WNC 2372); at the Australian National Museum, Sydney, NSW, Australia, 7 parthenogenetic females and 8 males on slides; and at the USDA Nematode Collection, Beltsville, MD, USA 3 parthenogenetic females and 2 males on slides. Description. Parthenogenetic female. Body shape variable, arcuate to open C-shaped, dorsally curved with ventral side convex and most curvature in tail region; relatively small; relatively broad; similar in size to amphimictic pre-parasitic females and smaller than males; body narrows behind vulva to form a conoid tail. With light microscope, cuticle appears smooth, sub-cuticle with strong longitudinal striae. Lateral fields not seen. Cephalic region ~ 70 % diameter of body at anterior end, offset, 2 µm high, unstriated; rounded outline in lateral view, circum-oral area flat or slightly raised. Amphids not seen. Stylet well sclerotised with cone occupying ~ 50 % of stylet length, basal knobs just higher than wide, 2–3 µm wide at base, rounded. Orifice of dorsal pharyngeal gland 1–2 µm posterior to stylet knobs. Anterior fusiform part of digestive tract occupying 38–66 % (mean 50 %, n = 11) of body diameter, length 2–3 times diameter; lumen of tract broadens at distal end of dorsal pharyngeal gland. Pharyngeal glands extending over intestine, occupying 50–76 % (mean 65 %) of body diameter, length from head to end of glands being 37 (30–50)% of total body length. Gland nucleus large, with medium-sized nucleolus. Holotype Partheno Parthenogenetic Males Infective females genetic female females Spicule length 18.4 ± 0.8 (17–19) Tail length 28 24 ± 3.2 45 ± 3.6 22 (18–32) (37–48) (21-23) Secretory/excretory pore opens anterior to nucleus of pharyngeal gland; secretory/excretory cell not seen. Hemizonid not seen. Reproductive tract variable in length, extending to secretory/excretory pore, part-way along pharyngeal gland or to nerve ring; flexed in 6 / 15 specimens examined; oviduct usually with two oocytes in a row; quadricolumella with clustered cells, uterus extensile, containing 1– 5 eggs; vulva a simple transverse slit with protruding rounded lips in some specimens; no vulval plate. Anus pore-like, opening in cuticular depression. Tail relatively short, conoid, concave on dorsal side; length 1.3–2.2 times anal body diameter; tip bluntly rounded. Infective pre-parasitic female. Arcuate shape when heat-relaxed; relatively broad; maximum body diameter at mid-body length; body tapers gradually in tail region. Cuticle obscurely annulated, less than 1 µm wide; longitudinal striae apparent with light microscope; lateral fields not seen. Cephalic region just offset, dome-shaped; circum-oral area rounded; stylet slender, weakly sclerotised with basal knobs being higher than wide; ~ 2 µm wide; cone ~ 40 % of stylet length. Orifice of dorsal pharyngeal gland 1–2 µm posterior to stylet knobs. Anterior fusiform part of digestive tract little expanded, occupying ~ 50 % of body diameter, length 2.0 – 2.5 times diameter. Pharyngeal glands occupying ~ 30 % of body diameter, extending over intestine, length from head to end of glands being ~ 30 % of total body length. Secretory/excretory pore opens behind pharyngeal glands; duct obscure; ellipsoid secretory/excretory cell ~ 7 µm long. Hemizonid not seen. Uterus ~ 70 % of total gonad length in uninseminated females, packed with sperm in inseminated females; vagina at right angle to body axis; reproductive tract extending to dorsal pharyngeal gland; hypertrophy of length of tract in some specimens. Vulva a transverse slit, vulval lips not raised, no vulval plate present. Anus an obscure pore. Tail relatively short, sub-cylindroid; length 1.3–1.5 times diameter at anus, tip broadly rounded. Male. Body arcuate when relaxed by heat, tail region slightly curved ventrally. Cuticle clearly annulated, annuli ~ 1 µm wide at mid-body length; strong longitudinal striae apparent with light microscope; lateral fields ~ 5 µm wide, lacking prominent lateral ridges, having broken diagonal striae. Cephalic region occupying ~ 70 %– 90 % anterior body diameter, offset, ~ 2 µm high; circum-oral area slightly raised, with lightly sclerotised framework; stylet well sclerotised with cone 30 % of length, stylet knobs higher than wide, 2–3 µm wide, rounded. Anterior fusiform part of digestive tract occupying 52–70 % of body diameter, length 2–3 times diameter. Orifice of dorsal pharyngeal gland ~ 2 µm behind knobs. Pharyngeal glands occupying 65 (47–82)% of body diameter, extending over intestine, length from head to end of glands being 33 (23–48)% of total body length. Lumen of intestinal tract broadens behind pharyngeal gland. Secretory/excretory pore opens opposite nucleus of pharyngeal gland; duct obscure; secretory/excretory cell ~ 5 µm long. Hemizonid lens-like, extending over two annules, 8 annules in front of secretory/excretory pore. Reproductive tract with single testis, variable in length, usually extending to nerve ring but may overlap dorsal pharyngeal gland; may be reflexed; testis, seminal vesicle and vas deferens not clearly differentiated. Bursa peloderan but may appear to be leptoderan, smooth; may be prominent or obscure; arises 50 % – 80 % along length of body. Spicules paired, angular at about half length, moderately sclerotised; with manubrium wider than shaft, may or may not be offset; blade narrows irregularly to bluntly rounded tip with concavity on distal edge; opening subterminal. Inconspicuous muscles associated with cloaca. Tail arcuate, ventrally concave, conoid; length 2.2–2.9 times diameter at cloaca; bluntly rounded tip. Molecular phylogenetic relationships. For molecular analysis, the partial SSU (V 410: FJ 393268), the D 2 / D 3 expansion segments of LSU (V 405: FJ 386994: V 410: FJ 386995) and mtCOI (V 405: FJ 386964) of F. armillarisae n. sp. were sequenced. Based on the LSU, V 405 and V 410 are close to each other and sister to V 412 (M. linariifolia n. sp.) (Davies et al., 2010 a, Fig. 78). A phylogenetic tree inferred from COI placed V 405 with V 462 (M. decora n. sp.) in a clade and in a basal position with many other Fergusobia species collected from Melaluca (Davies et al., 2010 a, Fig. 79). The blastn search of SSU of V 410 (1686 bp sequenced) revealed it has 7 to 11 -bp differences (99 % identity) from some Fergusobia species. The blastn search of LSU of V 410 (880 bp sequenced) revealed it has 9 -bp differences (94 % identity) and 3 gaps with V 405 and 40 -bp differences (95 % identity) and 16 gaps with V 412 (FJ 386996) (M. linariifolia n. sp.). The blastn search of COI of V 405 (618 bp sequenced) revealed the highest match was with V 462 (FJ 386982) (M. decora n. sp.) with 52 -bp differences (92 % identity). These large sequence divergences supported Fergusobia armillarisae n. sp. as a unique species. Diagnosis and relationships. Fergusobia armillarisae n. sp. is morphologically characterized by the combination of a medium-sized, arcuate parthenogenetic female with an extensile uterus, a narrow conoid tail; an arcuate, relatively broad, infective female with an almost hemispherical tail tip; and a small arcuate male with an angular spicule having an offset manubrium, and bursa arising at 50 %– 80 % of body length. Morphologically, F. armillarisae n. sp. is similar to F. linariifoliae n. sp., but lacks the swollen cuticle of the latter. The parthenogenetic female of F. armillarisae n. sp. (arcuate shape) differs from F. brevicauda Siddiqi, 1994, F. brittenae Davies, 2010 (in Taylor & Davies 2010), F. cosmophyllae Davies 2013 b (in Davies et al. 2013 b), F. diversifoliae Davies 2013 (in Davies et al. 2013 b), F. fasciculosae Davies 2012 (in Davies et al. 2012 b), F. floribundae 2013 (in Davies et al. 2013 b), F. gomphocephalae Davies 2014 (in Davies et al. 2014 c), F. indica (Jairajpuri, 1962) Siddiqi, 1986, F. leucoxylonae Davies 2014 (in Davies et al. 2014 c), F. magna Siddiqi 1986 sensu Davies 2010 (in Davies et al. 2010 b), F. microcarpae Davies 2013 (in Davies et al. 2013 a), F. minimus Lisnawita 2013 (in Davies et al. 2013 b), F. morrisae Davies 2012 (Davies et al. 2012 b), F. pimpamensis Davies 2013 (in Davies et al. 2013 b), F. planchonianae Davies 2014 (in Davies et al. 2014 b), F. porosae Davies 2013 (Davies et al. 2013 a), F. ptychocarpae Davies, 2008 (Taylor & Davies 2008), F. viminalisae Davies 2014 (in Davies et al. 2014 b), and F. viridiflorae Davies & Giblin-Davis, 2004 (C-shape). The presence of an extensile uterus in the parthenogenetic female of F. armillarisae n. sp. separates it from that of F. cajuputiae Davies & Giblin-Davis, 2004, F. colbrani Davies 2014 (in Davies et al. 2014 a), F. dealbatae Davies & Giblin-Davis, 2004, F. delegatensae Davies 2013 (in Davies et al. 2013 b), F. eugenioidae Davies 2012 (in Davies et al. 2012 b), F. fisheri Davies & Lloyd, 1996, F. leucadendrae Davies & Giblin-Davis, 2004, F. nervosa Davies & Giblin-Davis, 2004, F. philippinensis Siddiqi, 1994, F. schmidti Davies 2014 (in Davies et al. 2014 c), F. quinquenerviae Davies & Giblin-Davis, 2004, F. ro s et t a e Davies 2013 (in Davies et al. 2013 f), F. rileyi Davies 2011 (in Davies et al. 2011 a), F. sporangae Davies 2014 (in Davies et al. 2014 c), F. tolgarae Davies 2014 (in Davies et al. 2014 d), F. tumifaciens (Currie 1937) Wachek 1955 sensu Davies 2014 (in Davies et al. 2014 b), and F. decorae n. sp., which do not have extensile uteri. In having cuticle that does not swell upon fixation, it differs from F. jambophila Siddiqi 1986, F. linariifoliae n. sp. and F. pohutukawa Davies 2007 (in Taylor et al. 2007), in which it does. Having a flat or slightly raised circum-oral area separates the parthenogenetic female of F. armillarisae n. sp. and that of F. camaldulensae Davies 2011 (in Davies et al. 2011 a), in which it is distinctly raised. The stylet (9–12 µm) of this parthenogenetic female is longer than in F. curriei Fisher & Nickle 1968 (5–8 µm) and F. juliae Davies 2012 (in Davies et al. 2012 b) (5–7 µm). In having enormous oesophageal glands (b’ 1.5–2.2), it is similar to F. quinquenerviae but lacks the extra lobe or flex found in the gland of the latter. Infective females of F. armillarisae n. sp. have an arcuate shape, differing from that of F. diversifoliae, F. fasciculosae, F. gomphocephalae, F. leucadendrae, F. nervosae, F. pimpamensis, F. philippinensis, F. rosettae, F. sporangae, F. tolgarae, and F. viminalisae (open C-shape). With body length ranging from 279–291 µm, it is smaller than the female of F. brittenae (375–550 µm), F. colbrani (369–405 µm), F. cosmophyllae (374–448 µm), F. curriei (417–489 µm), F. dealbatae (307–347 µm), F. delegatensae (375–452 µm), F. eugenioidae (438 µm), F. floribundae (357–450 µm), F. juliae (396–550 µm), F. linariifoliae n. sp. (347–384 µm), F. magna (537–633 µm), F. microcarpae (302–341 µm), F. morrisae (322–395 µm), F. pimpamensis (369–443 µm), F. philippinensis (290–370 µm), F. planchonianae (303–339 µm), F. ptychocarpae (387–471 µm), F. sporangae (289–353 µm), and F. viminalisae (334–437 µm). In having a flat circum-oral area, the infective female of F. armillarisae n. sp. differs from those of F. camaldulensae, in which it is raised. It has a longer stylet than F. minimus and F. schmidti (respectively, 11–14 vs 4–5.5 and 5–10 µm). It has a short sub-cylindroid tail (21–23 µm) with an almost hemispherical tip, separating it from F. brevicauda (24–30 µm), F. d ec o r a e n. sp. (13–18 µm), F. f i s he r i (21–49 µm, mean 30 µm), F. leucoxylonae (11–18 µm), F. rileyi (40–50 µm with a bluntly rounded tip), and F. porosae and F. tolgarae in which the tip is bluntly rounded. Given that only two specimens of the infective female of F. armillarisae n. sp. were examined, it is difficult to separate it from the same stage collected from various broadleaved melaleucas. However, it appears to be shorter than F. viridiflorae (321 µm); and to have a more anterior secretory/excretory pore than F. cajuputiae and F. quinquenerviae (anterior end to secretory/excretory pore 47 µm vs 53–78 and 71–91 µm). In shape (arcuate), the male of F. armillarisae n. sp. differs from that of F. brittenae, F. curriei, and F. fasciculosae (J-shape), F. pimpamensis, F. schmidti (J or C-shape), F. magna, F. planchonianae, F. ptychocarpae, and F. viridiflorae (with strongly curved posterior). In length (302–400 µm), it is smaller than the male of F. diversifoliae (413–459 µm) and F. floribundae (403–570 µm). The male of F. armillarisae n. sp. has a flattened circum-oral area, but it is raised in F. camaldulensae, F. colbrani and F. jambophila. In length (9–12 µm), the stylet is shorter than in F. leucoxylonae (10–13 µm) and longer than in F. minimus (4–7 µm). The shape of the tail (arcuate with a bluntly rounded tip) differs from that of F. philippinensis (truncate tip). Spicule length (17–19 µm) is shorter than in F. juliae (20–27 µm). The shape of the spicules in F. armillarisae n. sp. (angular to arcuate) differs from those of F. brevicauda, F. cajuputiae, F. dealbatae, F. delegatensae, F. eugenioidae, F. gomphocephalae, F. leucadendrae, F. microcarpae, F. morrisae, F. pohutukawa, F. quinquenerviae, and F. viminalisae, in which it is clearly angular. In the male of F. armillarisae n. sp., the bursa arises at ~ 50 %– 80 % of the body length, separating it from those of F. cosmophyllae (~ 10 %– 40 %), F. decorae n. sp. (~ 30 %– 50 %), F. f i s h e r i (~ 20 %), F. linariifoliae n. sp. (80 %– 90 %), F. nervosae (~ 50 %), F. pohutukawa (~ 90 %), F. porosae (15 %– 33 %), F. rileyi (90 %– 95 %), F. rosettae (~ 40 %– 50 %), F. sporangae (10 %– 40 %) and F. tumifaciens (18 %– 22 %). Etymology. Named after M. armillaris, the plant species from which the nematodes were collected.Published as part of Davies, Kerrie A., Giblin-Davis, Robin M., Ye, Weimin, Taylor, Gary S., Makinson, Jeff & Purcell, Matthew, 2014, Nematodes from galls on Myrtaceae. X. Fergusobia from galls on narrow-leaved Melaleuca spp. in Australia, with descriptions of three new species, pp. 237-258 in Zootaxa 3889 (2) on pages 239-243, DOI: 10.11646/zootaxa.3889.2.4, http://zenodo.org/record/22465
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