1,721,057 research outputs found
“Alpha-anomeric configuration of GT-oligodeoxynucleotide leads to loss of the specific aptameric and cytotoxic properties retained by the beta-anomeric analogue.”
No full textEthoxylated polyethylenimine successfully enhances the cytotoxic activity of GT oligomers targeted to specific nuclear proteins in human T-Lymphoblastic cancer cells.
No full text“Can TIE-2 expressing monocytes represent a novel marker for Hepatocellular Carcinoma?
No full textHepatocellular carcinoma (HCC), the predominant form of primary liver cancer, is a global health problem representing the sixth most common cancer and the third cause of cancer related death worldwide. The number of deaths per year in HCC is comparable to the incidence number, underlying the aggressive behavior of HCC and the modest efficacy of available curative treatments. Effective HCC treatment is problematic also due to the lack of early and specific diagnostic markers. In this regard, particular interest has been put on the tyrosine kinase with Ig and EGF homology domains 2 (TIE2), a receptor of angiopoietins, predominantly present on endothelial cells but also observed on monocytes (TIE-2-expressing monocytes – TEMs). Recently, a work by Matsubara et al showed that the amount of circulating TEMs is higher in HCV/HCC patients compared to HCV patients or healthy subjects. Additionally the authors showed that TEMs have a diagnostic potential for HCC. Whereas the molecular mechanisms responsible for this observation remain elusive and further studies are necessary to confirm this finding, the work of Matsubara et al may contribute to the identification of a novel HCC prognostic and diagnostic marker
In vivo specific loss of DNA amplification by intermolecular triple-helix forming oligodeoxyribonucleotides.”
No full textUso di small interfering RNA (SIRNA) per il trattamento di patologie caratterizzate da iperproliferazione cellulare
No full text“Increase of therapeutic index of doxorubicin and vinblastine by aptameric oligonucleotide in human T-lymphoblastic drug-sensitive and multidrug-resistant cells.”
No full textTherapeutic potential of small interfering RNAs/micro interfering RNA in hepatocellular carcinoma
Full text linkHepatocellular carcinoma (HCC) is the predominant
form of primary liver cancer and represents the third
leading cause of cancer-related death worldwide.
Current available therapeutic approaches are poorly
effective, especially for the advanced forms of the
disease. In the last year, short double stranded RNA
molecules termed small interfering RNAs (siRNAs) and
micro interfering RNAs (miRNA), emerged as interesting
molecules with potential therapeutic value for HCC.
The practical use of these molecules is however limited
by the identification of optimal molecular targets and
especially by the lack of effective and targeted HCC
delivery systems. Here we focus our discussion on the
most recent advances in the identification of siRNAs/
miRNAs molecular targets and on the development of
suitable siRNA/miRNAs delivery systems
The Role of the eEF1A Family in Human Cancers
No full textOncogene proteins are proteins coded by oncogenes. They include proteins resulting from the fusion of an oncogene and another gene (oncogene proteins, fusion). An oncogene is a modified gene, or a set of nucleotides that codes for a protein and is believed to cause cancer. This book brings together the latest research in this field from around the world
Interaction of G-rich GT oligonucleotides with nuclear-associated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines.
No full textG-rich GT oligonucleotides with a different content of G clusters have beenevaluated for their ability to exert cytotoxicity and to bind to nuclear-associatedproteins in T-lymphoblast CCRF-CEM cells. Only the oligomersthat did not form G-based structures or had a poor structure, under physiologicalconditions, were able to exert significant cellular growth inhibitioneffect. The cytotoxicity of these oligomers was related to their bindingto the nuclear-associated eEF1A protein, but not to the recognition ofnucleolin or other proteins. In particular, GT oligomers adopting a conformationcompatible with G-quadruplex, did not exert cytotoxicity and didnot bind to eEF1A. The overall results suggest that the ability of oligomersto adopt a G-quadruplex-type secondary structure in a physiological buffercontaining 150 mm NaCl is not a prerequisite for antiproliferative effect inhaematopoietic cancer cells. The cytotoxicity of G-rich GT oligomers wasshown to be tightly related to their binding affinity for eEF1A protein
The more basic isoform of eEF1A relates to tumour cell phenotype and is modulated by hyper-proliferative/differentiating stimuli in normal lymphocytes and CCRF-CEM T-lymphoblasts
No full textThe elongation factor 1A proteins (eEF1A1/A2) are known to play a role in tumours. We
previously found that a more basic isoform of eEF1A (MBI-eEF1A) is present in the
cytoskeletal/nuclear-enriched extracts of CCRF-CEM T-lymphoblasts but not in those of
normal lymphocytes. To obtain deeper knowledge about MBI-eEF1A biology, we investigate
from which of the eEF1A proteins, eEF1A1 or eEF1A2, MBI-eEF1A originates and the
possibility that its appearance can be modulated by the differentiated or proliferative cell
status. CCRF-CEM T-lymphoblasts and normal lymphocytes were cultured with or without
differentiation/pro-proliferative stimuli (Phorbol 12-Myristate 13-Acetate (PMA) alone or the
combination of phytohaemagglutinin (PHA) with PMA, respectively), and the presence of
MBI-eEF1A evaluated together with that of the eEF1A1/A2 mRNAs. Our data indicate
that the MBI-eEF1A may derive from eEF1A1 as eEF1A2 is not expressed in CCRF-CEM
and normal lymphocytes. Moreover, MBI-eEF1A is inducible in normal lymphocytes upon
hyper-proliferative stimuli application; in CCRF-CEM, its presence can be abrogated by
PMA-induced differentiation. Finally, MBI-eEF1A may have a functional role in
hyper-proliferating/tumour cells as its disappearance reduces the growth of CCRF-CEM
and that of PHA/PMA-stimulated lymphocytes. The presented data suggest thatMBI-eEF1A
may be related to oncogenic cell phenotype, rising the possibility to use MBI-eEF1A as target
for novel therapeutic strategies
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