1,354,290 research outputs found
A Novel Precursor Ion Discovery Method on a Q-TOF Mass Spectrometer for GnRH Detection in Complex Biological Mixtures
No full text1,3-stereocontrol with bromoallenes. Synthesis of N-Boc-ADDA, the unique amino acid present in several inhibitors of serine/threonine phosphatases
No full textIn Vitro analysis of biological effects of Solanum lycopersicum (Tomato)-Derived Nanovesicles (TDNVs) as a potential therapeutic for wound treatment
No full textPlant-derived nanovesicles have been considered interesting in medicine for their breakthrough
biological effects, including wound healing. However, tomato-derived nanovesicles (TDNVs)
were not studied for their effects on wound closure yet. TDNVs were isolated from Solanum
lycopersicum (var. Piccadilly) ripe tomatoes by ultracentrifugation. Extract (collected during the
isolation procedure) and NVs (pellet) were characterized by transmission electron microscopy
and laser doppler electrophoresis. Wound healing in the presence of Extract or NVs was analysed
by a scratch assay with monocultures of human keratinocytes (HUKE) or NIH-3T3 mouse
fibroblasts. Cell proliferation and migration were studied by MTT and agarose spot assay,
respectively. The vesicles in the Extract and NVs samples were nanosized with a similar mean
diameter of 115 nm and 130 nm, respectively. Both Extract and NVs accelerated wound closure
of injured HUKE and NIH-3T3 monocultures, already at 6h post-injury. Although both samples
exerted no cytotoxic effect on HUKE and NIH-3T3 fibroblasts, they did not augment cell
proliferation. NVs and Extract increased cell migration of both cell types. NVs from tomatoes
may accelerate wound healing by increasing keratinocyte and fibroblast migration. These results
indicate the potential therapeutic usefulness of TDNVs in the treatment of chronic or hard-to-heal
ulcers
Stereoconvergent synthesis of (2S,3S,8S,9S,4E,6E)-N-Boc-ADDA starting from (S)-serine and (S)-phenyllactic acid
No full textThe important naturally occurring beta-amino acid N-Boc-ADDA is prepared following a disconnection of the C-C bond between the two E,E double bonds. The stereochemistry of the two synthons was controlled using the alkylation of chiral bromoalienes derived from naturally occurring (S)-serine and (S)-phenyllactic acid. The cupration of bromoallenes derived from (S)-serine also provides a general method for the synthesis of chiral beta-alkylated aspartic acid derivatives. (C) 1997, Elsevier Science Ltd
Occurrence of D-aspartate in the Harderian gland of Podarcis s. sicula and its effect on gland secretion
No full textHigh concentrations of free D-aspartate (D-Asp), an amino acid well known for its neuroexcitatory activity, are endogeneously present in the Harderian gland (HG) of the lizard Podarcis s. sicula. This orbital gland consists of two different parts: the medial part, which is prevalently a mucous acinar gland, and the lateral part, which is a serous tubulo-acinar gland. To determine the physiological effect of D-Asp on exocrine secretion in HG, D-Asp (2.0 μmol/g b.w.) was injected intraperitoneally into lizards. We found that highest accumulations of exogenous D-Asp in HGs occurred 15 hr after the injection. Specifically, exogenous D-Asp prevalently stimulated serous secretion from the lateral portion of the gland, where immunohistochemical analysis revealed a major accumulation. Similarly, in the medial part of the gland, highly sulfated mucosubstances were observed after D-Asp injection. Further, in both parts of the HG, the electron microscope revealed euchromatic nuclei, a prominent rough endoplasmic reticulum, as well as numerous secretory granules within the acinar cells. Thus, following D-Asp injection, a 60% increase in HG total protein was detected. In addition, exogenous D-Asp induced changes in the electrophoretic pattern of HG. In conclusion, although further investigations are still needed to clarify the molecular pathway induced by D-Asp in exocrine secretion, this study does indicate that free D-Asp plays a significant role in the secretory activity of this gland. © 2006 Wiley-Liss, Inc
Ignatius Loyola, from De vita et institvto S. Ignatii
No full textFrom Bartoli, Daniello. De vita et institvto S. Ignatii Societatis Iesv fvndatoris libri qvinqve, Lvgdvni : Sumptibus Lavrentii Anisson, 1665.
This beautiful engraved portrait of Ignatius is the frontispiece of a book in Grasselli\u27s Special Collections called On the Life and Institutes of St. Ignatius. The biography was written by Father Daniel Bartoli and was translated into Latin by Father Ludovico Ianino. The book was published in Lyons in 1665.https://collected.jcu.edu/jesuitimages/1001/thumbnail.jp
Stereocontrolled synthesis of gamma-branched amino acids. TiCl4 mediated addition of (E)-crotylsilane to N,O-protected serine aldehyde
No full textThe N-Boc derivatives of (2S,3S,4R,6E)-2-amino-3-hydroxy-4-methyl-6-octenoic acid and (2S,4R)-2-amino-4-methyl-hexanoic acid have been prepared using the acetonide of D-Serine aldehyde 1 as a formyl glycine equivalent. The stereochemistry of the y-branch was introduced by reaction of (E)-crotyltrimethylsilane with 1 in the presence of TiCl4 followed by elaboration of the terminal double bond and further transformation of the Serine hydroxymethyl group into a carboxylic acid. Copyright (C) 1996 Elsevier Science Lt
D-aspartate modulates transcriptional activity in Harderian gland of frog, Rana esculenta: Morphological and molecular evidence
No full textIn the green frog, Rana esculenta, a substantial amount Of D-aspartate (D-Asp) is found enclogenously within the Harderian gland (HG) following its synthesis from (L)-aspartate ((L)-Asp) by an aspartate racemase. The frog HG is an orbital seromucoid gland that displays seasonal changes in secretory activity. Our in vivo experiments, consisting of i.p. injection of 2.0 mu mol/g.b.w. (D)-Asp in frogs collected during two periods of differing glandular activity (high or medium-low secretory activity), revealed that HG can to take up and accumulate D-Asp and that this amino acid may modulate the exocrine secretion through a kinase pathway. Atatirnewhenthe gland shows relatively low secretory activity, i.p. administration of (D)-Asp rapidly induced activation of ERK1 and an increase in cells active in RNA synthesis. This increase in transcriptional activity was followed by a significant increase in Mucous secretion. By contrast, administration of exogenous (D)-Asp when HG was showing high activity rapidly induced inhibition of both ERK1 and transcriptional activity. Since D-Asp is known to be recognized by receptors for N-methyl-(D)-aspartic acid (NMDA), it is possible that in the HG, D-Asp mediated NMDA activation may enhance the kinase pathway. The above activation of opposing stimulatory and inhibitory processes could reflect different levels of NMDA-receptor activity, which could vary as a function of the level of gland activity. This study provides the first evidence of a role for this excitatory amino acid in exocrine secretion. The effects of (D)-Asp in HG appear to be specific since they were not seen in frogs treated with other (D)- or (L)-amino acids with known excitatory effects on neurosecretion. (c) 2005 Wiley-Liss, Inc
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