3 research outputs found
Daniel Pierce Thompson and “The Shaker Lovers”: Portraying the Shakers in Fiction and on the Stage
No full textWhen Vermont author D. P. Thompson’s short story “The Shaker Lovers” was first published in the periodical The New World in 1841, it joined a growing body of literature on American Shaker communities. However, Thompson’s story was unusual in three ways. First, it was the product of a man whose upbringing and opinions gave him a unique perspective on the Shakers. Second, it was a fictional literary treatment of the Shakers, of which there had been very few up to that date. And last, Thompson’s story was destined to be turned into a stage play, albeit one with a somewhat different set of messages than in the original story
Erratum to: MiR-9-5p Down-Regulates PiT2, but not PiT1 in Human Embryonic Kidney 293 Cells
No full textThe original version of this article unfortunately contained mistakes in the Author group and Abstract sections. Author D. P. Bezerra’s name was incorrectly presented as “D. P. Paiva”. This abstract version substitutes the previous one: Abstract PiT1 (SLC20A1) and PiT2 (SLC20A2) are members of the mammalian type-III inorganic phosphate transporters and recent studies linked SLC20A2 mutations with primary brain calcifications. MicroRNAs (miRNAs) are endogenous noncoding regulatory RNAs and MicroRNA-9 (miR-9) modulates neurogenesis but is also involved with different types of cancer. We evaluated possible interactions between miR-9 and the phosphate transporters (PiT1 and PiT2). SLC20A2, platelet-derived growth factor receptor beta (PDGFRB) and Fibrillin-2 (FBN2) showed binding sites with high affinity for mir-9, In silico. miR-9 mimic was transfected into HEK293 cells and expression was confirmed by RT-qPCR. Overexpression of miR-9 in these cells caused a significant reduction in PiT2 and FBN2. PDGFRB appeared to be decreased, but was not significantly down-regulated. PiT1 showed no significant difference relative to controls. The down-regulation of PiT2 protein by miR-9 was confirmed by western blotting. In conclusion, we showed that miR-9 can down-regulate PiT2, in HEK293 cells
