52 research outputs found
A case of embryonal rhabdomyosarcoma of the cheek in adolescence. Its cytological diagnosis by fine-needle aspiration via the gingival fornix
No full textAbstract
The authors present a case of embryonal rhabdomyosarcoma of the cheek in a young patient. The histological diagnosis, on the tumoral mass, was preceded by cytological researches with fine needle aspiration biopsy, carried out on the gingival fornix. The cytology, by immunocytochemical techniques, made possible the diagnosis of rhabdomyosarcoma. Therefore the authors stress the high capacity of these cytochemical and immunocytochemical methods to demonstrate, not in a invasive way, the small round cell tumors of the skull and the neck in the youngs. This early diagnosis may orient the clinical and therapeutic management of the case
Localization of hepatitis C virus antigen(s) by immunohistochemistry on fixed-embedded liver tissue
No full textUsing two sources of primary antibodies, we immunohistochemically stained hepatitis C virus-related antigen(s) on fixed-embedded liver specimens. These antigens were localized in the cytoplasm of hepatocytes. The results obtained serologically correlated well with immunohistochemistry
Clinically silent pancreatic "Somatostatinoma" in MEN - 1 syndrome, and literature review.
No full textAltered lipid peroxidation/glutathione ratio in experimental extrahepatic cholestasis.
No full text1. Lipid peroxidation can occur in the presence of a cellular antioxidant-oxidant imbalance, but the role of lipid peroxides in cholestasis is not well understood.
2. This study was undertaken in order to: (i) evaluate the behaviour of a product of lipid peroxidation (thiobarbituric acid-reactive species), and of an important antioxidant tripeptide, reduced glutathione, in the course of experimental extrahepatic cholestasis; and (ii) ascertain whether there was a link between this aspect and the alterations in liver morphology.
3. Forty-five male Sprague-Dawley rats (250-300 g) were double bile duct ligated and followed from 1 to 28 days. At the end of each experimental period, blood and liver samples were collected for thiobarbituric acid-reactive species and glutathione determinations.
4. Bile duct ligated rats showed a marked increase in liver weight which was related to cholestasis duration and to some anatomical alterations such as bile duct proliferation and dilation and liver fibrosis (periportal, perivenular, perineoductular and parenchymal).
5. An increase in serum lipid peroxidation was also observed but this was not linked to hepatic thiobarbituric acid-reactive species. Erythrocyte and hepatic glutathione decreased in relation to cholestasis duration. Serum lipid peroxides and erythrocyte glutathione were correlated with liver cell necrosis.
6. In conclusion, experimental extrahepatic cholestasis determines bile duct proliferation and fibrosis, the degree of which is directly related to the duration of cholestasis itself and to liver cell necrotic phenomena. Furthermore, extrahepatic cholestasis is associated with increased lipid peroxide formation and with a depletion of reduced glutathione both in the liver and in the erythrocytes. The alteration in the oxidative balance may be a contributory factor in necrotic liver cell phenomena
Malignant mixed müllerian tumor of the endometrium. Report of a case with cytological diagnosis.
No full textA case is reported of malignant mixed mesodermal tumor of the uterus in a postmenopausal 56 year-old woman presenting vaginal bleeding and who was cytologically diagnosed with an endometrial aspirate. The cytologic examination of smears showed the presence of two different cellular types: adenocarcinomatous and sarcomatous (some with evident cross striations). The patient underwent total hysterectomy with lymphadenectomy. Histologically, a malignant mixed Müllerian tumor was confirmed. Cytological, histological, histochemical and immunohistochemical features are described in detail together with the main differential diagnosis
Localization of hepatitis C virus (HCV) antigen by immunohistochemistry on fixed-embedded liver tissue
No full textCytokines and the progression of liver damage in experimental bile duct ligation
No full text1. Cytokines are soluble factors whose action has been documented in physiological and pathological conditions. Some may be involved in the pathogenesis of cholestasis, whether of acute or chronic origin. 2. The aim of the present study was to evaluate the influence of epidermal growth factor (EGF), transforming growth factor (TGF)-β1 interleukin (IL)-6 and tumour necrosis factor (TNF) on cholestasis. Findings from Sprague-Dawley rats submitted to bile duct ligation for 1-28 days were compared with those from controls, which underwent laparotomy but not bile duct ligation. 3. Biochemical and morphological findings confirmed that the experimental procedure was successful. At the end of each follow-up period, the hepatic levels of the cytokines were determined and compared with liver histology findings. 4. The four cytokines studied showed different patterns of activation: hepatic levels of EGF, higher in the experimental than the control group, were comparable with the proliferative picture. The TGF-β1 pattern was correlated with data of periportal, perivenular and perineoductular fibrosis, confirming that this cytokine has a role in mediating the synthesis of matrix proteins. A fluctuating, phasic pattern was found for TNF in the experimental group, with high values on day 0, a decrease on the first and second postoperative days and then two peaks on days 8 and 14. Finally, immediately after surgical manipulation, high levels of IL-6 were found in the experimental group, followed by a decrease in levels until zero values were obtained. 5. This suggests that the obstructive condition produces several cytokine responses, each of which contributes to determine the cholestatic condition
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