461 research outputs found
Frédérique Neau-Dufour, Yvonne de Gaulle
No full textComme le rappelle Frédérique Neau-Dufour dès la première ligne de la biographie qu’elle consacre à l’épouse du général de Gaulle, « dans les mémoires, Yvonne de Gaulle est demeurée un personnage falot ». L’image qui vient spontanément à l’esprit est celle de la femme du président de la République, en retrait de son grand homme de mari. Une image tronquée et simplifiée qui, malgré l’effacement et la discrétion volontaire de l’intéressée, ne rend pas compte de cette personnalité peu prolixe, d..
A Streptomyces sp. NEAU-HV9: Isolation, Identification, and Potential as a Biocontrol Agent against Ralstonia solanacearum of Tomato Plants
No full textRalstonia solanacearum is an important soil-borne bacterial plant pathogen. In this study, an actinomycete strain named NEAU-HV9 that showed strong antibacterial activity against Ralstonia solanacearum was isolated from soil using an in vitro screening technique. Based on physiological and morphological characteristics and 98.90% of 16S rRNA gene sequence similarity with Streptomyces panaciradicis 1MR-8T, the strain was identified as a member of the genus Streptomyces. Tomato seedling and pot culture experiments showed that after pre-inoculation with the strain NEAU-HV9, the disease occurrence of tomato seedlings was effectively prevented for R. solanacearum. Then, a bioactivity-guided approach was employed to isolate and determine the chemical identity of bioactive constituents with antibacterial activity from strain NEAU-HV9. The structure of the antibacterial metabolite was determined as actinomycin D on the basis of extensive spectroscopic analysis. To our knowledge, this is the first report that actinomycin D has strong antibacterial activity against R. solanacearum with a MIC (minimum inhibitory concentration) of 0.6 mg L−1 (0.48 μmol L−1). The in vivo antibacterial activity experiment showed that actinomycin D possessed significant preventive efficacy against R. solanacearum in tomato seedlings. Thus, strain NEAU-HV9 could be used as BCA (biological control agent) against R. solanacearum, and actinomycin D might be a promising candidate for a new antibacterial agent against R. solanacearum
Central Station power switching characteristics
No full textThis ATM summarizes the conditions which led to the ALSEP Central Station Discrepancy Report AA 7344 and analyzes the results of trouble shooting steps performed to isolate and define the anomaly. Recommendations are made regarding proper operational procedures.prepared by D. Douthat, O. Neau, W. Tosh
Histoire des souffrances du sieur Elie Neau, sur les galères, et dans les cachots de Marseille, Édition et présentation de Didier Poton et Bertrand Van Ruymbeke
No full textElie Neau, marin français naturalisé anglais et condamné aux galères (1693-1698), est l’un des 1551 « galériens pour la foi », c’est-à-dire des forçats protestants des années 1680-1748. Il est connu par l’Histoire abbreggee des soufrances du sieur Elie Neau, sur les galères, et dans les cachots de Marseille, Rotterdam, 1701, dont voici une belle réédition. L’ouvrage s’ouvre par une très riche introduction. Celle-ci analyse tout d’abord la spécificité éditoriale de l’ouvrage publié du vivant d..
Data_Sheet_1_Dimeric Pimprinine Alkaloids From Soil-Derived Streptomyces sp. NEAU-C99.PDF
No full textSix new pimprinine alkaloids (1–6), including four dimers, dipimprinines A–D (1–4), and two monomers, (±)-Pimprinol D (5), and pimprinone A (6), along with six known congeners (7–12), were isolated from a soil-derived actinomycete Streptomyces sp. NEAU-C99. Structures of the new compounds were elucidated by extensive spectroscopic analyses, single-crystal X-ray diffractions, and ECD calculations. Dipimprinines A–D (1–4) showed weak cytotoxic activities against five tumor cell lines, including HL-60, SMMC-7721, A-549, MCF-7, and SW-480, with IC50 values ranging from 12.7 to 30.7 μM.</p
Streptomyces triticagri sp. nov. and Streptomyces triticirhizae sp. nov., two novel Actinobacteria isolated from the rhizosphere soil of wheat (Triticum aestivum L.)
No full textTwo novel Actinobacteria , designated strains NEAU-YY421T and NEAU-YY642T, were isolated from the rhizosphere soil of wheat (Triticum aestivum L.) collected from Zhumadian, Henan Province, PR China and characterized using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains NEAU-YY421T and NEAU-YY642T belonged to the genus Streptomyces and strain NEAU-YY421T was most closely related to Streptomyces fumanus CGMCC 4.1732T (97.9 % sequence similarity) and Streptomyces naganishii DSM 40282T (97.8 %), and that of strain NEAU-YY642T to Streptomyces zhaozhouensis LZS-5T (98.0 %) and Streptomyces sedi YIM 65188T (97.5 %). The cell walls of the two strains contained ll-diaminopimelic acid as the diagnostic diamino acid and the whole-cell hydrolysates were glucose and ribose. Multilocus sequence analysis using the concatenated sequences of the atp D, gyr B, rec A, rpo B and trp B genes showed that the two strains formed separate branches in the genus Streptomyces . Moreover, a combination of DNA–DNA hybridization results and cultural and physiological properties indicated that the two strains can be distinguished from their closest phylogenetic relatives. Therefore, strains NEAU-YY421T and NEAU-YY642T belong to two novel species in the genus Streptomyces , for which the names Streptomyces triticagri sp. nov. (NEAU-YY421T=CGMCC 4.7476T=DSM 106775T) and Streptomyces triticirhizae sp. nov. (NEAU-YY642T=CCTCC AA 2018092T=DSM 107172T) are proposed, respectively
Molecular basis of the fructose-2,6-bisphosphatase reaction of PFKFB3: Transition state and the C-terminal function
No full textThe molecular basis of fructose-2,6-bisphosphatase (F-2,6-P 2ase) of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB) was investigated using the crystal structures of the human inducible form (PFKFB3) in a phospho-enzyme intermediate state (PFKFB3-P•F-6-P), in a transition state-analogous complex (PFKFB3•AlF 4), and in a complex with pyrophosphate (PFKFB3•PP i) at resolutions of 2.45, 2.2, and 2.3 Å, respectively. Trapping the PFKFB3-P•F-6-P intermediate was achieved by flash cooling the crystal during the reaction, and the PFKFB3•AlF 4 and PFKFB3•PP i complexes were obtained by soaking. The PFKFB3•AlF 4 and PFKFB3•PP i complexes resulted in removing F-6-P from the catalytic pocket. With these structures, the structures of the Michaelis complex and the transition state were extrapolated. For both the PFKFB3-P formation and break down, the phosphoryl donor and the acceptor are located within ∼5.1 Å, and the pivotal point 2-P is on the same line, suggesting an in-line transfer with a direct inversion of phosphate configuration. The geometry suggests that NE2 of His253 undergoes a nucleophilic attack to form a covalent N-P bond, breaking the 2O-P bond in the substrate. The resulting high reactivity of the leaving group, 2O of F-6-P, is neutralized by a proton donated by Glu322. Negative charges on the equatorial oxygen of the transient bipyramidal phosphorane formed during the transfer are stabilized by Arg252, His387, and Asn259. The C-terminal domain (residues 440-446) was rearranged in PFKFB3•PP i, implying that this domain plays a critical role in binding of substrate to and release of product from the F-2,6-P 2ase catalytic pocket. These findings provide a new insight into the understanding of the phosphoryl transfer reaction. © 2011 Wiley Periodicals, Inc
A present from a farr countrey, to the people of New England. I. A great voice from heaven, to these parts of the earth: in an excellent letter full of divine rarities, lately written from a terrible prison in France; / by a pious confessor of the reformed religion, once an inhabitant of this countrey. ; (With some late remarkables, of the persecution, upon the reformed in that kingdom.) II. The golden bells of the great high priest, heard from heaven, through the land. Or, Meditations, upon the methods of grace, wherein a few faithful persons may be the happy instruments of delivering a land, from all its iniquities & calamities.
No full text53, [1] p. ; 15 cm. (8vo)Attributed to Cotton Mather by Holmes.Parentheses substituted for square brackets in statement of responsibility.Contains a letter in French from Elias Neau, translated by Nehemiah Walter of Roxbury."A brief discourse, made unto the Great and General Assembly ... 21d. 8m. 1697."--p. 22-53.Erratum note, p. 53.Bookseller's advertisement, p. [54
Mise au point d une méthode prédictive pour le calcul des équilibres de phases des systèmes eau - hydrocarbures - glycols
No full textLorsque de l eau est présente dans un fluide de gisement pétrolier elle entraîne, dans les conditions de température et pression sous-marines, l apparition de démixtions et l éventuelle formation d hydrates d hydrocarbures. La prévention de la formation de ces hydrates peut se faire par l ajout de composés tels que des glycols. Un modèle prédictif dénommé NRTL-PR, associant l équation d état de Peng Robinson et le modèle d enthalpie libre d excès NRTL, à été développé au cours de ce travail. Celui-ci a été élaboré de manière à pouvoir représenter aussi bien les équilibres liquide vapeur et les enthalpies de mélange des mélanges d hydrocarbures, que les équilibres liquide liquide des systèmes eau hydrocarbures et éthylène glycol hydrocarbures. Les résultats obtenus avec notre modèle ont été comparés à ceux du modèle PPR78, pour les mélanges d hydrocarbures et de gaz permanents, et à ceux du modèle RK-PR, pour les mélanges impliquant de l eau ou de l éthylène glycol. Ces comparaisons se montrent favorables au modèle NRTL-PR.AIX-MARSEILLE3-BU Sc.St Jérô (130552102) / SudocSudocFranceF
Crystal structure of heart 6‐phosphofructo‐2‐kinase/fructose‐2,6‐bisphosphatase (PFKFB2) and the inhibitory influence of citrate on substrate binding
No full text© 2016 Wiley Periodicals, Inc. The heart-specific isoform of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB2) is an important regulator of glycolytic flux in cardiac cells. Here, we present the crystal structures of two PFKFB2 orthologues, human and bovine, at resolutions of 2.0 and 1.8 Å, respectively. Citrate, a TCA cycle intermediate and well-known inhibitor of PFKFB2, co-crystallized in the 2-kinase domains of both orthologues, occupying the fructose-6-phosphate binding-site and extending into the γ-phosphate binding pocket of ATP. This steric and electrostatic occlusion of the γ-phosphate site by citrate proved highly consequential to the binding of co-complexed ATP analogues. The bovine structure, which co-crystallized with ADP, closely resembled the overall structure of other PFKFB isoforms, with ADP mimicking the catalytic binding mode of ATP. The human structure, on the other hand, co-complexed with AMPPNP, which, unlike ADP, contains a γ-phosphate. The presence of this γ-phosphate made adoption of the catalytic ATP binding mode impossible for AMPPNP, forcing the analogue to bind atypically with concomitant conformational changes to the ATP binding-pocket. Inhibition kinetics were used to validate the structural observations, confirming citrate\u27s inhibition mechanism as competitive for F6P and noncompetitive for ATP. Together, these structural and kinetic data establish a molecular basis for citrate\u27s negative feed-back loop of the glycolytic pathway via PFKFB2. Proteins 2016; 85:117–124. © 2016 Wiley Periodicals, Inc
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