1,721,054 research outputs found
Raman spectroscopy of osteosarcoma cells
Full text linkOsteosarcoma is the most common primary malignant bone tumor. In the last years, several studies have demonstrated that the increase of Hydroxyapatite (HA) and Interleukin-6 (IL-6) syntheses compared to those expressed by normal osteoblasts could be used to detect the degree of malignancy of osteosarcoma cells. Conventional biochemical methods widely employed to evaluate bone cell differentiation, including normal and cancerous phenotypes, are time consuming and may require a large amount of cells. HA is a mineral form of calcium phosphate whose presence increases with maturation of osteosarcoma cells. Analogously, IL-6 is a fundamental cytokine whose production is highly increased in osteosarcoma cells. In this study, we employ Raman spectroscopy to the identification and discrimination of osteosarcoma cells from osteo-differentiated mesenchymal stromal cells (MSCs) by detecting the presence of HA and IL-6. However, while the identification of HA is facilitated by the characteristic peak at 960 cm-1, corresponding to symmetric stretching (P-O) mode, the quantification of IL-6 it is much more elusive, being its Raman signal characterized by cysteine, but also by phenylalanine, amide I II and III whose signals are common to other proteins. Supported by an accurate multivariate analysis, the results show that Raman spectroscopy is a high sensitivity technique dealing out a direct and quantitative measurement of specific mineralization levels of osteosarcoma cells. In turn, by exploiting the Surface-Enhanced Raman Scattering stimulated by internalized Gold Nanoshells (AuNSs) and combined with scanning probe microscopies, we were able to employ Raman spectroscopy to study subcellular components locally
Cellular distribution of RhoA in human embryonic and adult renal tissues and renal cell lines: morphofunctional implications.
No full textTissue engineering in the reconstructive challenges of the middle ear
No full textTissue engineering in the reconstructive challenges of the middle ea
Effects of barium titanate nanoparticles on proliferation and differentiation of rat mesenchymal stem cells
No full textNanomaterials hold great promise in the manipulation and treatments of mesenchynal stem cells, since they allow the modulation of their properties and differentiation. However, systematic studies have to be carried out in order to assess their potential toxicological effects. The present study reports on biocompatibility evaluation of glycol-chitosan coated barium titanate nanoparticles (BTNPs) on rat mesenchymal stem cells (MSCs). BTNPs are a class of ceramic systems which possess interesting features for biological applications thanks to their peculiar dielectric and peizolelctric properties. Viability was evaluated up to 5 days of incubation (concentration in the range 0-100 ug/ml) both quantitatively and qualitatively with specific assays. Interactions cells/nanoparticles were further investigated with analysis of the cytoscheleton conformation, with SEM and TEM imaging, and with AFM analysis. Finally, differentiation in adipocytes and osteocytes was achieved in the presence of high doses of BTNPs, thus highlighting the safety of these nanostructures towards mesenchymal stem cell
New prostheses for middle ear ossicles obtained by tissue engineering strategies
No full textNew prostheses for middle ear ossicles obtained by tissue engineering strategie
Clot as a biological scaffold for mesenchymal stem cells in treatment of orthopaedic healing
No full textTissue engineered constructs as human ossicular chain replacements
No full textTissue engineered constructs as human ossicular chain replacement
Ossicular chain replacements using human mesenchymal stem cells cultured on PORP-like scaffolds based on poly propylene fumarate
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