6,581 research outputs found

    Jc-1 assay`

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    JC-1 assay results for 24 hour Alexidine dihdyrochloride for Panc-1 cell line. This assay is a supporting assay, not included in any manuscript

    GMT-20-script-JC-ETOPO1-MT

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    GMT-20-script-JC-ETOPO1-M

    Introduction

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    Results for testing JC.

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    <p>Correlation of current prediction with what happened on the nth trial in the past plotted against trials into the past (n). JC shows a stronger influence of recent trials than normal controls, and normal controls show a stronger influence of distant trials than JC.</p

    jc.2021-01642.R1_supplemental file.docx

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    Supplemental file to article jc.2021-0164

    JC-VLP uptake in spleen.

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    <p>Panels A–I: Mouse tissues were perfusion fixed 7 min after intravenous injection of JC-VLPs. Paraffin sections of spleen were double immune labeled using a rabbit antiserum against BK-VP1 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111762#pone.0111762-Rinaldo1" target="_blank">[53]</a> (reacts also with JC-VP1), and antibodies against F4/80 (A–C), CD31 (D–F) or CD163 (G–I). Antibodies are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111762#pone-0111762-t001" target="_blank">Table 1</a>. The VP1 staining pattern (green) showed that the uptake of VLPs was concentrated in the red pulp marginal zone, mz (A), and here partly co-localized with F4/80 (C, arrow), and CD31 (F, arrows), but not with CD163 (I; arrow indicates VP1, and arrow head to CD163 staining). The F4/80 (B, C), CD31 (E, F), and CD163 (H, I) staining patterns are all shown in red. Panels J–L: In J–L, tail vein injection of JC-VLPs was followed by injection of FITC-FSA in the opposite tail vein and tissues perfusion fixed 15 min after the VLP administration. Both JC-VLP (K; red fluorescence) and FITC-FSA (J; green fluorescence) distributed to the reticuloendothelial network of the spleen red pulp marginal zone (L; arrows point to overlap of JC-VLP staining and FITC-FSA uptake).</p

    Electron microscopy of BK- and JC-virus like particles (VLPs).

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    <p>The figure show transmission electron micrographs of negatively stained BK- and JC-VLPs constructed from BK-VP1 or JC-VP1 proteins: A) BK-VP1 VLPs (Dunlop strain); B) JC-VP1-Mad-1 VLPs; C) JC-VP1-L55F VLPs; D) JC-VP1-S269F VLPs. Scale bars: 200 nm.</p

    JC-1 assay.

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    <p>JC-1 mitochondrial depolarization assay after control, S, T, or S+T treatment of U87 and U373 CSCs.</p
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