3,454 research outputs found
Steven Yedinak Interview
No full textLTC (RET) Steven M. Yedinak commissioned in the U. S. Army Infantry in 1963 and subsequently spent 26 years in Special Forces and Airborne Infantry. He served two combat tours in Vietnam (1966-67 & 1971-1972), and started the Mobile Guerrilla Force. He is the author of Hard to Forget: An American with the Mobile Guerrilla Force in Vietnam (Random House, 1998). He retired from the Army in 1989
Steven M. Lowenstein Collection - Addenda. 1960s-2007
No full textThe Steven Lowenstein Collections documents professional activities of Steven Lowenstein, writer, researcher, historian, and teacher. Documents comprising the collection reflect his interests in a wide spectrum of topics related to Jews and Judaism, such as modernity and tradition and their influence on the religion and common folks; Berlin Jews of the upper strata; similarities and differences between agrarian/rural and urban Jews; popular and official Judaism; secular and religious Jews; and other Jewish related topics. However, there is a very small amount of materials related to his professional activities other than research and writing.There are two distinct types of materials – correspondence and manuscripts: Correspondence collected here pertain to Steven Lowenstein’s writings and publishing efforts; his teaching activities; conferences; writings by others; and research projects. Additional correspondence dealing with Steven Lowenstein’s editorial work, writings, and publishing efforts is found in Series II: Writings.Writings collected here range from large scale works to short essays, reviews and lectures. By and large, his manuscripts are accompanied by very extensive supplementary materials such as notes, research files, correspondence with publishers regarding publications and publication’s rights, small amount of correspondence with colleagues, and printed materials. Additionally, there are legal documents, such as agreements and permissions; small amount of printed materials, and photographs.Steven Lowenstein, scholar, teacher, and a writer was born in New York in 1945 into a family of German –Jewish refugees. He received his master’s degree from Princeton University in 1969 and went on earning a doctorate degree from Princeton University in 1972. He taught at a number of universities, including Columbia University and Monmouth College, and worked as a researcher at YIVO and the Leo Baeck Institute. In the late 1970s Dr. Lowenstein moved to California where he taught Jewish history at the American Jewish University. He is the author of a large number of scholarly works, including The Jewish Cultural Tapestry: International Jewish Folk Traditions; The Berlin Jewish Community: Enlightenment, Family and Crisis, 1770-1830; and Frankfurt on the Hudson: The German-Jewish Community of Washington Heights, 1933-1983, Its structure and Culture.digitize
Landsat MSS classification of fire fuel types in Wood Buffalo National Park, northern Canada
No full textJ1: Global Ecology & Biogeography Letters; M3: Article; Milne, David Franklin, Steven E. Wilson, Bradley A. Ghitter, Geoff Heathcott, Mark McCaffrey, Thomas M. Ow, Charlotte F. Y.; Source Information: Mar1994, Vol. 4 Issue 2, p33; Subject Term: FOREST fires; Author-Supplied Keyword: Canada (Wood Buffalo National Park); Author-Supplied Keyword: Forest fire; Author-Supplied Keyword: Fuel type classification; Author-Supplied Keyword: Landsat data; Number of Pages: 0p; Document Type: Articl
Steven Bryant’s Solace: a conductor’s analysis and performance guide
No full textThe purpose of this study was to examine Solace, a musical composition for wind ensemble, by Steven Bryant composed for the University of North Carolina at Greensboro Wind Ensemble and premiered at the 2013 College Band Directors National Association National Conference. Through a conductor's analysis and performance guide, the author provided insight and background knowledge to all future performers and interpreters of the work through unique first hand accounts from commission to premiere performance. The research method included three processes: 1. A detailed analysis of the musical score, 2. The observation of rehearsals and recording sessions during preparation for the premiere performance of Solace by the University of North Carolina at Greensboro Wind Ensemble, Kevin M. Geraldi, conductor, 3. Extensive interviews of Steven Bryant, composer and Kevin M. Geraldi, conductor. Through examination of prior research on electro-acoustic works for wind ensemble, the author examined Solace within those constructs. Because of the blurring of lines between electronics and acoustic sound, the author further identified Solace as a unique musical composition within the electro-acoustic genre
Supplemental Material - Outcomes of Injured Patients Before and After the Institution of a Third-Tier Activation at a Level 1 Trauma Center
No full textSupplemental Material for Outcomes of Injured Patients Before and After the Institution of a Third-Tier Activation at a Level 1 Trauma Center by Andrew M. Young, Brian R. Young, Steven D. Young, Alexander Brundage, Prajeeth Koyada, Christopher Cramer, and Jeffrey S Young in The American Surgeon™</p
Author Correction: Identification of functional tetramolecular RNA G-quadruplexes derived from transfer RNAs
No full textThe original version of this Article contained an error in the spelling of the author Steven M. Coyne, which was incorrectly given as Stephen M. Coyne. This has now been corrected in both the PDF and HTML versions of the Article.</jats:p
Mylothris agathina subsp. agathina agathina (Cramer 1779
No full text<i>Mylothris agathina agathina</i> (Cramer, 1779) <p>Larsen 1996: pl. 11, figs 105 i,ii. d’ Abrera 1997: 113 (3 figs). SI: Figure 34g –j.</p> <p> Forewing length: male 29–35 mm (mean (<i>n</i> = 5) 32.08 mm, SD = 1.921); female 28.5–35 mm (mean (<i>n</i> = 7) 31.84 mm, SD = 1.908).</p> <p> <i>Records.</i> Kielland (1990, p.65) considered this butterfly to be widespread throughout Tanzania, occurring in deciduous woodlands, forest margins and clearings, even gardens, from sea level to 2200 m. Rogers (1908, p.537) recorded a single female from Kilimanjaro, collected 26–31 January 1906, apparently at about 5000 ft elevation (c. 1600 m); this specimen, together with several males and females from Taveta, is preserved at OUMNH. One male and three females were reported from Kibongoto, Kilimanjaro, by Aurivillius (1910a, p.11), occurring at up to 1200 m, where the species was encountered in April and December. Specimens in BMNH include several from Engaruka (about 100 km west of Kilimanjaro), and a single male from Old Moshi. On this basis, <i>M. agathina</i> is included as part of the lower slopes fauna.</p> <p> Beyond Tanzania, nominate <i>agathina</i> is very widespread in the eastern half of Africa, from Sudan south to South Africa. A second subspecies occurs in Cameroon, Central African Republic, Uganda and Ituri (DRC) (Ackery et al. 1995).</p> <p>This is a dimorphic species, the male upperside ground colour being pure white, while most females are a vibrant orangey-yellow – although they can be paler, with some almost white, male-like. Females, on average, have slightly larger “dotted border” spots on the hindwings.</p>Published as part of <i>Liseki, Steven D. & Vane-Wright, Richard I., 2014, Butterflies (Lepidoptera: Papilionoidea) of Mount Kilimanjaro: family Pieridae, subfamily Pierinae, pp. 1543-1583 in Journal of Natural History 48 (25 - 26)</i> on page 1573, DOI: 10.1080/00222933.2014.886343, <a href="http://zenodo.org/record/5193830">http://zenodo.org/record/5193830</a>
High-throughput characterization of colloidal properties of monoclonal antibodies
No full textAugust 2016School of EngineeringIn this work, we have significantly improved the AC-SINS method and used it for differentiating between mAbs with different levels of self-association in serum as well as in other formulation solutions. We find that the AC-SINS measurements are well correlated with several conventional antibody biophysical properties (solubility, aggregation) and non-conventional biophysical properties (non-specific interactions with non-adsorptive chromatography columns). Moreover, we have identified sequence features of antibodies that are responsible for their variable biophysical properties, and find strong correlation between our self-interaction measurements and computational predictions based on the sequences of the antibody binding loops. These findings highlight the potential of using high-throughput biophysical methods for rapid identification and optimization of mAbs for high concentration therapeutic applications.Monoclonal antibodies (mAbs) are currently the focus of intense interest for therapeutic applications due to their potent bioactivities. However, the process of identifying and developing mAbs into approved therapeutics is an extremely challenging task. One of the key challenges is to identify mAb candidates with excellent biophysical properties, including high solubility (colloidal stability) and low viscosity at elevated antibody concentrations required for subcutaneous delivery. However, it is difficult to characterize the biophysical properties of mAbs early in the discovery process due to the large number of antibody candidates (hundreds to thousands) as well as their low concentrations (micrograms per mL) and purities (cell culture supernatants). We aim to address some of these challenges by developing a high-throughput assay (affinity-capture self-interaction nanoparticle spectroscopy, AC-SINS) capable of measuring weak antibody colloidal interactions that is compatible with extremely dilute and unpurified mAb solutions. AC-SINS uses gold nanoparticles coated with polyclonal antibodies to capture the target human mAbs on the surface of the particles, which amplifies antibody self-interactions due to polyvalency. Attractive mAb self-interactions reduce interparticle separation distances between conjugates, which leads to red shifts in the plasmon wavelength (wavelength of maximum absorbance).Ph
Strategies for the development of integrated purification processes for non-platform biologic therapeutics
No full textAugust 2017School of EngineeringIn the first approach, a multiscale platform for the discovery of peptide affinity chromatography ligands was developed and applied for the purification of human growth hormone (hGH) from yeast cultures. A high-throughput batch screening approach using RP-UPLC was created to rapidly sample portions of the competitive adsorption isotherms for the biologic, allowing the selection of peptide leads based on their abilities to both bind the product from representative mixtures and subsequently desorb it from the chromatographic resin. A peptide ligand, SMWRTYH, was selected as the final candidate for the purification of hGH and in column-scale experiments was shown to purify hGH from yeast (Pichia pastoris) cultures with a product recovery of 80% and purity of 95% after a single step.The successful implementation of the strategies presented in this thesis provides evidence that a characterization of both the product and the impurity interactions with the separations media can enable the development of highly constrained, integrated purification processes for non-platform biologics. Moreover, this body of work offers perspective on the relative merits and drawbacks of the various strategies that can be employed to design these processes.Finally, a more straightforward approach was used to develop an integrated purification process for an Interferon mutant. This approach combined the lessons learned from the impurity characterization experiments with the results from a gradient elution screen on the product and its key variants to guide process development. These results demonstrate that integrated purification processes can also be designed for molecules with significant (>30%) product variant separation challenges and that a characterization of the host-related impurities can be used to guide decisions in the development process, even without the use of the in-silico process synthesis tool.The second process development approach made use of a novel method for characterizing the host-related impurities in Pichia cultures. This characterization data was analyzed by an in silico tool which synthesized tentative chromatographic purification processes and ranked them based on predicted impurity clearance. The utility of the approach was demonstrated by employing it to develop integrated, 3-step purification processes for two therapeutic proteins using only commercially available separations media. This impurity characterization based approach could have applications in the development of highly constrained purification processes for a wide range of non-platform biologics.The design of chromatographic purification processes for non-platform biologic therapeutics is a complex problem due to the multitude of species involved and the difficulty of predicting their interactions with the separations media. This design problem is made even more challenging if the target therapeutic is to be manufactured in an integrated fashion, in which hold tanks, buffer conditioning, and other steps which allow the design of each unit operation on an individual basis are removed. This thesis explored several strategies to develop purification processes for non-platform biologics which were to be manufactured in a small-scale, integrated system with no conditioning steps or hold tanks between chromatographic unit operations.Ph
M. Patrick Graham & Steven L. McKenzie (ed.), The Chronicler as Author. Studies in Text and Texture, Sheffield, Sheffield Academic Press, 1999, (Journal for the Study of the Old Testament, Supplement Series, 263), ISBN 1-84127-057-1
No full textRobert Philippe de. M. Patrick Graham & Steven L. McKenzie (ed.), The Chronicler as Author. Studies in Text and Texture, Sheffield, Sheffield Academic Press, 1999, (Journal for the Study of the Old Testament, Supplement Series, 263), ISBN 1-84127-057-1. In: Revue d'histoire et de philosophie religieuses, 80e année n°2, Avril-juin 2000. p. 297
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