1,721,034 research outputs found
THE LEVEL OF INDUCED SCES SHOWS AN ANOMALOUS PATTERN OF EXTINCTION IN DEMETHYLATED CHINESE-HAMSTER OVARY CELLS
No full textDNA damage repair and genetic polymorphisms: assessment of individual sensitivity and repair capacity
No full textPurpose: To study the repair capacity after X-ray irradiation in human peripheral blood cells of healthy subjects, in relation to their genotypes.
Methods and Materials: The peripheral blood of 50 healthy subjects was irradiated in vitro with 2Gy of X rays and the induced DNA damage was measured by Comet assay immediately after irradiation. DNA repair was detected by analyzing the cells at defined time intervals after the exposure. Furthermore, all subjects were genotyped for XRCC1, OGG1, and XPC genes.
Results: After X-ray irradiation, persons bearing XRCC1 homozygous variant (codon 399) genotype exhibited significantly lower Tail DNA values than those bearing wild-type and heterozygous genotypes. These results are also confirmed at 30 and 60 min after irradiation. Furthermore, XPC heterozygous subjects (variant codon 939) showed lower residual DNA damage 60 min after irradiation compared with wild-type and homozygous genotypes.
Conclusion: The results of the present study show that polymorphisms in DNA repair genes could influence individual DNA repair capacity. (c) 2006 Elsevier Inc
Damage proneness induced by genomic DNA demethylation in mammalian cells cultivated in vitro
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Influence of XRCC1 Genetic Polymorphisms on Ionizing Radiation-Induced DNA Damage and Repair
Full text linkIt is well known that ionizing radiation (IR) can damage DNA through a direct action, producing single- and double-strand breaks on DNA double helix, as well as an indirect effect by generating oxygen reactive species in the cells. Mammals have evolved several and distinct DNA repair pathways in order to maintain genomic stability and avoid tumour cell transformation. This review reports important data showing a huge interindividual variability on sensitivity to IR and in susceptibility to developing cancer; this variability is principally represented by genetic polymorphisms, that is, DNA repair gene polymorphisms. In particular we have focussed on single nucleotide polymorphisms (SNPs) of XRCC1, a gene that encodes for a scaffold protein involved basically in Base Excision Repair (BER). In this paper we have reported and presented recent studies that show an influence of XRCC1 variants on DNA repair capacity and susceptibility to breast cancer
RESVERATROL ACTS AS A TOPOISOMERASE IIα POISON: INDUCTION OF DNA DAMAGE AND INHIBITION OF CHROMOSOME SEGREGATION
No full textRecent advances in pharmacological therapy of Parkinson's disease: levodopa and carbidopa protective effects against DNA oxidative damage
No full textINFLUENCE OF SPERMIDINE ON SISTER CHROMATID EXCHANGES INDUCED BY ALKYLATING-AGENTS IN MAMMALIAN-CELLS INVITRO
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