1,721,321 research outputs found
Whole Exome for the Identification of Mutations in CD8+ T-Cells
No full textAdvances in next-generation sequencing and in particular whole exome sequencing (WES) have provided an innovative opportunity to perform a mutational screening of the entire coding region of the genome down to the single base, enhancing the discovery of causal mutations important for disease treatment and management. Recently, the accumulation of germline mutations in expanded CD8+ T-cells has been found to have a pathogenic significance in autoimmune diseases such as rheumatoid arthritis, and, on the other hand, this type of mutations may act in combination with newly acquired somatic mutations modulating tumorigenesis, evolution, and cancer recurrence determining the clinical outcome. Therefore, we describe a protocol for identifying and characterizing germline single nucleotide variants (SNVs) and small deletions (Indels) from next-generation WES data of CD8+ T-cells coming from patients with autoimmune diseases and comparing them to matching control samples. Conversely, the same protocol can be applied for identifying and characterizing germline SNVs from CD8+ T-cells isolated from tumor samples with a non-favorable clinical outcome compared to those from patients with a favorable clinical outcome used as controls
Folder 8: Schwiderski, Richard Craig v. State of Texas 1, 1979-1984
No full textA notarized affidavit dated December 22, 1980 from Sharon L. Cox stating that she will not be able to finish a transcript in time for a deadline
Folder 8: Schwiderski, Richard Craig v. State of Texas 1, 1979-1984
No full textA notarized affidavit dated February 6, 1981 from Sharon L. Cox stating that she needs an extension of time to create a transcript
Folder 8: Schwiderski, Richard Craig v. State of Texas 1, 1979-1984
No full textA carbon copy and a copy of a bill dated March 10, 1981 to Donald J. Maison for transcription services by Sharon L. Cox in the State of Texas v. Richard Craig Schwiderski case
Folder 2: Court Documents 1, 1980-1981
No full textA transcript of a trial written by Sharon L. Cox July 29, 1980 before the court in the State of Texas v. Orval David Austin
Introduction
No full textThis introduction presents an overview of the key concepts discussed in the subsequent chapters of this book. The book focuses on the ethical consequences of different translation strategies, this time to convey the memory of perpetrators of mass political violence. It examines the commemoration of the 2004 siege of School No.1 in Beslan, North Ossetia-Alania. The book reviews a project titled Refugee Tales, involving symbolic walks that link histories of asylum seekers in Britain to iconic memory sites such as Chaucer’s The Canterbury Tales, Kafka’s ‘The Metamorphosis,’ and Magna Carta. Working across the verbal and the visual, Michelle Liao reveals how the contrasting narrative choices of the exhibition catalogue and text panels are driven by stakeholder attempts to distance the Chinese visitor from a traumatic moment in collective memory, and to distance the English-speaking visitor from the problematic Victorian gaze of the photographer
Heritage interpretations : remembering, translating, and utilizing the past
Full text linkThis chapter addresses the synergistic relationship between heritage, memory, and translation from a conceptual and an applied perspective. It begins by emphasizing how all three operations can be framed in relation to narratives, interactions, subjectivities, and shifts; and yet there remains significant interdisciplinary scope for further investigation into the myriad ways in which translation mediates heritage meanings and simultaneously negotiates visitor experience and engagement. Similarly, little space has been carved out for practical translation considerations within heritage interpretation guidelines, and so this chapter calls for more sustained, empirically led, and joined-up thinking about the heritage–memory–translation triad. The chapter then gestures towards the participatory turn in the cultural sector as a framework for bringing together various stakeholders in that triad to encourage a collaborative approach to interpretative materials. It reports on the endeavours of the 'Translating Scotland's Heritage' research network to stimulate cross-sectoral discussions and provides an applied case study on translation at the Kelpies heritage site. Central issues include anxieties over translation quality, the role of technology, the reception of translated material, accessibility, and minority language use. The chapter concludes with some reflections on directions in which the research horizon might expand
Biomarkers and Precision Medicine in IgA Nephropathy
No full textThe field of biomarker research in IgA nephropathy has experienced a major boost in recent years with the publication of a large number of scientific reports. Candidate biomarkers from blood, urine, and renal tissue obtained through the use of clinical chemistry, molecular biology, and omics have been proposed for translation in clinical practice. Nevertheless, individual biomarkers often lack sensitivity and specificity with the consequent impairment of disease specificity. This review, moving on from the analysis of the four-hit hypothesis, illustrates the biomarkers linked to the abnormal glycosylation process of IgA1 and the immune complex formation. It also describes other serum and urinary biomarkers. Given the profound insights into the pleiotropic function of a single biomarker that is specific for a pathophysiological mechanism, this review suggests a novel approach based on a panel of biomarkers that covers the entire pathogenic process of the disease. Clinical bioinformatics that integrate genetic, clinical, and bioinformatics data sets could optimize the specific value of each biomarker in a multimarker panel. This is a promising approach for precision medicine and personalized therapy in IgA nephropathy. (C) 2018 Elsevier Inc. All rights reserved
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