3 research outputs found
Immobilization of laccase on hybrid layered double hydroxide
No full textCrystals of Mg/Al layered double hydroxide were synthesized by alkaline precipitation and treated in an aqueous solution of glutamic acid. The glutamate ions were not intercalated into the interlayer space, but were detected in the material by Fourier transform infrared spectroscopy, suggesting that only the external surfaces of crystals were modified with glutamate ions. The resulting hybrid material was tested as a support for immobilization of the enzyme lacease (Myceliophthora thermophild). The immobilized enzyme preparation was characterized by electronic paramagnetic resonance spectroscopy and by assays of catalytic activity. The activity of the immobilized lacease was 97% of the activity in the free enzyme. Layered double hydroxide is a suitable support for use in remediation of soil studies
Immobilization of laccase on hybrid layered double hydroxide
No full textCrystals of Mg/Al layered double hydroxide were synthesized by alkaline precipitation and treated in an aqueous solution of glutamic acid. The glutamate ions were not intercalated into the interlayer space, but were detected in the material by Fourier transform infrared spectroscopy, suggesting that only the external surfaces of crystals were modified with glutamate ions. The resulting hybrid material was tested as a support for immobilization of the enzyme lacease (Myceliophthora thermophild). The immobilized enzyme preparation was characterized by electronic paramagnetic resonance spectroscopy and by assays of catalytic activity. The activity of the immobilized lacease was 97% of the activity in the free enzyme. Layered double hydroxide is a suitable support for use in remediation of soil studies
The use of Digital Image Correlation to correct the thermoelastic curves in static tests
No full textAbstractMany studies have been carried out with the aid of thermal scanners for the analysis of the thermoelastic effect on materials. In particular, for tensile stresses, the gradient is negative and the thermal variations are proportional to the stress applied in the purely elastic phase. Thermoelastic analysis of materials from the static tests have shown that it is possible to detect information on the dynamic (fatigue) behavior.One of the main problems that occur in following the local thermal behavior in a static test is that of dimensional correction and tracking of the measuring point (spot). These do not allow to follow the same investigated area, that progressively undergoes a displacement as load increases.The purpose of this work, based on previous experiences, is to implement an algorithm and to define a procedure that allows the tracking of the area thermally investigated that, as the load increasing, inevitably undergoes a displacement. The radiometric spots on the specimen, selected at the beginning of test, are progressively updated by following thermographic frames. The displacement information derived from the Digital Image Correlation (D.I.C.), simultaneously was applied on the same specimens. This procedure can better define the trend of temperature changes during the static tensile test and can be used to measure the stress concentration factors in notched specimens
