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    Environmental variability and allostatic load in the Eurasian red squirrel Sciurus vulgaris

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    Abstract: The analysis of eco-physiological parameters in wildlife may represent a priority for their conservation and management, not only to establish causal relationships, obtaining a set of raw data, but also to monitor the effectiveness of management strategies. We studied Hair Cortisol concentration (HCC) in Eurasian red squirrels Sciurus vulgaris in four selected study areas in Italy. Areas differed from one another for vegetation type, degree of urbanization and anthropic disturbance, density of red squirrel populations and presence or absence of alien squirrel species. HCC measurements showed the highest values in a high-density population, subjected to a high predation pressure (i.e. in areas characterized by deciduous vegetation). Conversely, the lowest HCC levels have been observed in areas with the lowest squirrel density, i.e. in urbanized environments, where the species is established for a rather long time, and in areas with mixed vegetation and presence of conifers. Measuring HCC provides reliable information on the long-term activity of hypothalamic–pituitary–adrenal (HPA) axis and associated allostatic load. Furthermore, it constitutes a non-invasive method of investigation. The use of hair tubes further supports our non-invasive approach. Because of its simplicity and low costs, hair collection might be applied to monitor the long-term physiological response of wild species even in larger areas, besides focusing in the promotion of citizen-science data collection. Graphic abstract: [Figure not available: see fulltext.]

    Isolated teratozoospermia: a cause of male sterility in the era of ICSI?

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    Single structural defects involving the totality of ejaculated sperm are among rare cases of untreatable human male infertility. This form of infertility is of genetic origin and is generally transmitted as an autosomal recessive traits. Acrosome agenesis or globozoospermia results from perturbed expression of nuclear proteins or from an altered Golgi-nuclear recognition during spermiogenesis. Failed fertilization after intracytoplasmic sperm injection (ICSI) of acrosomeless sperm is consistent with an inability of sperm to activate oocytes. Acephalic spermatozoa result from a head-neck defect due to a failure of migration of the tail anlagen and related centriole to the caudal pole of spermatids. An abnormal sperm centrosome function may explain the defective embryo cleavage after ICSI with sperm carrying a fragile head-neck junction. Primary cilia dyskinesia (PCD) and dysplasia of the fibrous sheath (DFS) are isolate defects associated with absent or greatly reduced sperm motility due to an abnormal ciliary structure and function (PCD) or to a disorganized fibrous sheath (DFS). Numerous defective genes are potentially involved in human isolated teratozoospermia but such defects have not been defined at the molecular level in most cases. IVF-ICSI is the only available method for obtaining live births with sperm carrying these defects, but the outcome is poor and the genetic risk for the subsequent generation can not be determined
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