1,720,993 research outputs found
Overexpression of Osmyb4 enhances compatible solute accumulation and increases stress tolerance of arabidopsis thaliana
No full textIn this paper, we report the metabolic and molecular changes in response to cold and drought induced in Osmyb4 transgenic Arabidopsis thaliana compared with the wildtype (WT). The rice Osmyb4 gene codes for a transcription factor (Myb4) induced by cold treatment and, in Arabidopsis transgenic plants, improves cold and freezing tolerance [Vannini C, Locatelli F, Bracale M, Magnani E, Marsoni M, Osnato M, Mattana M, Baldoni E, Coraggio I (2004) Plant J 37: 115-127]. Here, we report the ability of Myb4 to induce also drought tolerance in Arabidopsis transgenic plants. By the use of nuclear magnetic resonance (NMR) and enzymatic assays, we showed that several compatible solutes (glucose, fructose, sucrose, proline, glycine betaine and sinapoyl malate) accumulate in higher amount in Osmyb4-overexpressing plants with respect to the WT, both under normal and stress conditions. Considering proline, we also found that in transgenic plants the levels of the mRNAs coding for Δ1- pyrroline-5-carboxylate synthase (EC not assigned) and for Δ1- pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12) were higher and lower, respectively. The constitutive activation of several stress-inducible pathways and different kinetics in the accumulation of several metabolites, in Myb4 transgenic plants, may represent an advantage to prepare plants to face the stress condition. Moreover, these results taken together suggest that Myb4 integrates the activation of multiple components of stress response
Uso di specifici geni Myb per la produzione di piante transgeniche tolleranti gli stress biotici e abiotici
No full textLa presente invenzione riguarda l'uso del gene di riso Osmyb4 e dei suoi omologhi funzionali per l'ottenimento di piante con aumentata tolleranza a stress biotici ed abiotic
Use of di specific Myb genes for the production of the transgenic plants tolerant to biotic and abiotic stresses
No full textUso del fattore trascrizionale Mybleu per la produzione di piante transgeniche tolleranti gli stress abiotici
No full textLa presente invenzione riguarda l'uso del gene di riso Osmyb4 e dei suoi omologhi funzionali per l'ottenimento di piante con aumentata tolleranza a stress abiotic
The product of the rice myb7 unspliced mRNA dimerizes with the maize leucine-zipper opaque2 and stimulates its activity in transient expression assay
No full textmyb7 mRNA is present in rice in spliced and unspliced forms, splicing being enhanced by anoxia. The protein (Mybleu) encoded by the unspliced mRNA is composed of an incomplete Myb domain followed by a leucine zipper; however, it lacks canonical sequences for DNA binding, transcriptional activation, and nuclear localization. We show here that in transiently transformed tobacco protoplasts, Mybleu is able to enhance the transcriptional activity of the maize leucine zipper Opaque2 on its target b32 promoter. The Mybleu transactivation effect is strictly dependent on the presence of Opaque2 and is driven by Mybleu-Opaque2 heterodimers. Mybleu is located in the nucleus, both in rice and in transformed tobacco protoplasts. In rice, the protein is expressed in regions corresponding to undifferentiated cells of roots and coleoptiles. Therefore, myb7 mRNA encodes, depending on its splicing, two transcription factors belonging to separate classes. One of them, Myblue, has novel structural characteristics, suggesting the existence of new mechanisms acting in the activation of transcription.
-------------------------------------------------------------------------------
Use of specific Myb genes for the production of transgenic plants tolerant to biotic and abiotic stresses.
No full text
Osmyb7 of rice: one gene encodes for two transcription factors belonging to different classes
No full text- …
