77 research outputs found
Facade Leasing Demonstrator Project: 4.2.6.FLD D3. Business Delivery Report
No full textIncentivising investment and long-term collaboration in high-performance facadeprojects. This technical report is an annex to the Facade Leasing Demonstrator Project 2019 performance report(2.7.3.FLD.D1). For general information on the Facade Leasing research project, its process, and objectives please refer to the aforementioned document. This technical delivery report focuses on the economic and business model aspects of the FLD project. The innovation behind the Facade Leasing research project lies not so much in the creation of new, energy-efficient facade technologies, but rather the creation of new investment and management processes leading to amore widespread and effective use of available and upcoming technology. The present report starts by describing the large, and growing, market for economically feasible facade renovation solutions. The research presently focuses on the Dutch non-residential, (semi-)publicly owned market, which has been identified as an ideal early adopter, but extrapolations are made to other European segments in the “Upscaling” chapter. The report then presents the work done by the research and practice consortium of the FLD project, represented by real estate owners/operators, facade fabricators, financial institutions, and other key stakeholders towards the definition of a promising business and financial model for the contracting of Facades-as-a-Service.Building Product InnovationReal Estate Managemen
Facade Leasing Demonstrator Project: 2.7.3.FLD D4. Final Dissemination Activities Report
No full textScientific and professional dissemination of the Facade Leasing Demonstrator Project. This technical report is an annex to the Facade Leasing Demonstrator Project 2019 performance report(2.7.3.FLD.D1). For general information on the Facade Leasing research project, its process, and objectives please refer to the aforementioned document. The focus of the resent report is the dissemination and expansion of knowledge related to the FLD project. This includes academic work such as scientific publications, conferences, and master graduate thesis; as well as professional knowledge-sharing and networking events addressing the knowledge triangle of academy, industry, and research. The sensitive political and strategic nature of the CiTG building demonstrator case throughout the decision-making and execution period in 2018 and 2019 has prevented the external dissemination of specific information regarding the case-study. A draft plan has been setout, however, for a broader dissemination of the case as a large scale success story in early 2020, now that the project’s construction has been successfully finalised.Building Product InnovationReal Estate Managemen
Composition Analysis of Free Fatty Acids from Swertia Species by a Novel Pre-column Fluorescence Labelling Method Using HPLC-FLD
No full textFree fatty acids (FFA) are basic and indispensable components of medicinal plants. Many researches indicate that the efficacy of medicinal plant requires the presence of FFA. In the present study, ultrasonic-assisted extraction of FFA from Swertia species was optimized by response surface methodology (RSM), ensuring the highest FFA recoveries. A novel pre-column fluorescence labelling method using 2-(5-benzoacridine)ethyl-p-toluenesulfonate (BAETS) as a labelling reagent has been developed for highly sensitive and selective analysis of FFA by HPLC with fluorescent detection (FLD) and online mass spectrometry identification. RSM was also employed to optimize the fluorescence labelling of FFA. HPLC separation of 17 FFA derivatives was carried out on a reversed-phase Hypersil BDS C-8 column (4.6 x 200 mm, 5 mu m, Agilent Co.) with a gradient elution. The detection limits and quantifications were as low as 0.60 and 1.22 ng mL(-1), respectively. FFA analysis from nine Swertia species was performed by the newly developed method, and the FFA composition of these Swertia samples was first reported
Forming Limit Diagram Generation of Aluminum Alloy AA2014 Using Nakazima Test Simulation Tool
No full textAbstractPlastic instability is a commonly observed problem, in sheet metal forming operation, which leads to defective products. Forming Limit Diagram (FLD) is an important parameter to be considered in the manufacturing process of non-defective sheet products. This paper focuses on FLD prediction based on simulation of Nakazima test using finite element software Pam-Stamp 2G. Finite Element Model (FEM) for Nakazima test is established in this work. Then the experimental values are compared with the simulation results in order to establish the credibility of Nakazima test simulation tool. Then the simulation is extended to predict the FLD of AA2014 aluminium alloy
Lecithin: cholesterol acyltransferase activation by sulfhydryl-reactive small molecules: role of cysteine-31
No full textLecithin:cholesterol acyltransferase (LCAT) catalyzes plasma
cholesteryl ester formation and is defective in familial lecithin:
cholesterol acyltransferase deficiency (FLD), an autosomal recessive
disorder characterized by low high-density lipoprotein,
anemia, and renal disease. This study aimed to investigate the
mechanism by which compound A [3-(5-(ethylthio)-1,3,4-thiadiazol-
2-ylthio)pyrazine-2-carbonitrile], a small heterocyclic amine, activates
LCAT. The effect of compound A on LCAT was tested in human
plasma andwith recombinant LCAT.Mass spectrometry and nuclear
magnetic resonance were used to determine compound A adduct
formation with LCAT. Molecular modeling was performed to gain
insight into the effects of compound A on LCAT structure and
activity. Compound A increased LCAT activity in a subset (three of
nine) of LCATmutations to levels comparable to FLD heterozygotes.
The site-directed mutation LCAT-Cys31Gly prevented activation by compound A. Substitution of Cys31 with charged residues (Glu, Arg,
and Lys) decreased LCAT activity, whereas bulky hydrophobic
groups (Trp, Leu, Phe, and Met) increased activity up to 3-fold (P,
0.005).Mass spectrometry of a tryptic digestion of LCAT incubated
with compound A revealed a 1103.017 m/z adduct on Cys31,
consistent with the addition of a single hydrophobic cyanopyrazine
ring. Molecular modeling identified potential interactions of compound
A near Cys31 and structural changes correlating with
enhanced activity. Functional groups important for LCAT activation
by compound A were identified by testing compound A derivatives.
Finally, sulfhydryl-reactive b-lactams were developed as a new class
of LCAT activators. In conclusion, compound A activates LCAT,
including some FLD mutations, by forming a hydrophobic adduct
with Cys31, thus providing a mechanistic rationale for the design of
future LCAT activators
Inorganic arsenic causes fatty liver and interacts with ethanol to cause alcoholic liver disease in zebrafish
No full textThe rapid increase in fatty liver disease (FLD) incidence is attributed largely to genetic and lifestyle factors; however, environmental toxicants are a frequently overlooked factor that can modify the effects of more common causes of FLD. Chronic exposure to inorganic arsenic (iAs) is associated with liver disease in humans and animal models, but neither the mechanism of action nor the combinatorial interaction with other disease-causing factors has been fully investigated. Here, we examined the contribution of iAs to FLD using zebrafish and tested the interaction with ethanol to cause alcoholic liver disease (ALD). We report that zebrafish exposed to iAs throughout development developed specific phenotypes beginning at 4 days post-fertilization (dpf), including the development of FLD in over 50% of larvae by 5 dpf. Comparative transcriptomic analysis of livers from larvae exposed to either iAs or ethanol revealed the oxidative stress response and the unfolded protein response (UPR) caused by endoplasmic reticulum (ER) stress as common pathways in both these models of FLD, suggesting that they target similar cellular processes. This was confirmed by our finding that arsenic is synthetically lethal with both ethanol and a well-characterized ER-stress-inducing agent (tunicamycin), suggesting that these exposures work together through UPR activation to cause iAs toxicity. Most significantly, combined exposure to sub-toxic concentrations of iAs and ethanol potentiated the expression of UPR-associated genes, cooperated to induce FLD, reduced the expression of as3mt, which encodes an arsenic-metabolizing enzyme, and significantly increased the concentration of iAs in the liver. This demonstrates that iAs exposure is sufficient to cause FLD and that low doses of iAs can potentiate the effects of ethanol to cause liver disease. This article has an associated First Person interview with the first author of the paper
Façade Leasing Demonstrator Project: Business Delivery Report
No full textThis technical report is an annex to the Facade Leasing Demonstrator Project 2018 performance report (4.2.6.FLD.D1). For general information on the FacadeLeasing research project, its process, and objectives please refer to the aforementioned document. This technical delivery report focuses on the economic and business model aspects of the FLD project. As has been mentioned before, the innovation behind the Facade Leasing research project lies not so much in the creation of new, energy-efficient facade technologies, but rather the creation of new investment and management processes leading to a more widespread and effective use of available and upcoming technology. The present report starts by describing the large, and growing, market for economically viable facade renovation solutions. The research presently focuses on the Dutch non-residential, (semi-)publicly owned market, which has been identified as an ideal early adopter, butextrapolations are made to other European segments in the “Upscaling” chapter. The report then presents the work done by the research and practice consortium of the FLD project represented by real estate owners/ operators, facade fabricators, financial institutions, and other key stakeholders towards the definition of a promising business and financial model for the contracting of Facades-as-a-Service.Building Product InnovationReal Estate Managemen
The multidimensional optimal order detection method in the three-dimensional case : very high-order finite volume method for hyperbolic systems
No full textThe Multidimensional Optimal Order Detection (MOOD) method for two-dimensional geometries has been introduced by the authors in two recent papers.We present here the extension to 3D mixed meshes composed
of tetrahedra, hexahedra, pyramids, and prisms. In addition, we simplify the u2 detection process previously
developed and show on a relevant set of numerical tests for both the convection equation and the Euler system
that the optimal high order of accuracy is reached on smooth solutions, whereas spurious oscillations
near singularities are prevented. At last, the intrinsic positivity-preserving property of the MOOD method
is confirmed in 3D, and we provide simple optimizations to reduce the computational cost such that the
MOOD method is very competitive compared with existing high-order Finite Volume methods.The third author research was financed by FEDER Funds through Programa Operacional Factores de
Competitividade — COMPETE and by Portuguese Funds through FCT — Fundação para a Ciência e a
Tecnologia, within the Project PEst-C/MAT/UI0013/2011
Facade Leasing Demonstrator Project. Technical Delivery Report: Annex 4.2.6. FLD D2
No full textAccelerating deep building energy retrofits within the Circular Economy transition. This technical report is an annex to the Facade Leasing Demonstrator Project 2018 performance report (4.2.6.FLD.D1). For general information on the Facade Leasing research project, its process, and objectives please refer to the aforementioned document.This technical delivery report focuses on the design, engineering, construction, and monitoring process towards the energy retrofit of the East facade of the building of the Civil Engineering and Geo-sciences faculty at TU Delft (CiTG in Dutch). After building an initial prototype in November 2018, on one of the building’s typical office spaces, plans are to continue with the full retrofit of theEast facade of the building throughout 2019. The CiTG case is representative of a massive volume of buildings across Europe - over 50% according to some estimates - which have been built during the post-second world war period, and which are currently reaching the end of their original service life. Such buildings need urgent technical intervention in order to improve their energy, safety, and indoor comfort performance. Such interventions, however, must be realized in line with Circular Economy principles, as they demand the strategic investment of immense amount of resources: material, financial, and human. Resources which we cannot afford to keep using under a linear mentality of take - make - dispose.Building Product InnovationReal Estate Managemen
Facade Leasing Demonstrator Project: 2.7.3.FLD D2. Final Technical Delivery Report
No full textThis technical report is an annex to the Facade Leasing Demonstrator Project 2019 performance report(2.7.3.FLD.D1). For general information on the Facade Leasing research project, its process, and objectives please refer to the aforementioned document. This technical delivery report focuses on the design, engineering, construction, and monitoring process towards the energy retrofit of the East facade of the building of the Civil Engineering and Geo-sciences faculty at TU Delft (CiTG in Dutch). Having built an initial prototype in November 2018, on one of the building’s typical office spaces, the project continued with the execution of a full large-scale retrofit of the East facade of the building throughout 2019.The CiTG case is representative of a massive volume of buildings across Europe - over 50% according to someestimates - which have been built during the post-second world war period, and which are currently reaching the end of their original service life. Such buildings need urgent technical intervention in order to improve their energy, safety, and indoor comfort performance. Such interventions, however, must be realized in line with Circular Economy principles, as they demand the strategic investment of immense amount of resources: material, financial, and human. Resources which we cannot afford to keep using under a linear mentality of take - make -dispose.Building Product InnovationReal Estate Managemen
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