170,079 research outputs found
Institutions, innovation and performance in Guangdong firms: The role of entrepreneurial orientation and environmental turbulence
No full textThis study combines the institution-based perspective and the innovation systems literature to examine the role of public policies in the entrepreneurship-innovation-performance relationships. It does so by presupposing a key mediating role for entrepreneurial orientation and by introducing environmental turbulence as a moderator. Data from a sample of 166 Guangdong Province manufacturing firms are used to test hypotheses, which distinguish between institutional pressures and incentives. Findings reveal the lesser relevance of institutional pressures whereas a more virtuous relationship between institutional incentives, entrepreneurial orientation and both innovation and overall performance emerges, especially when environmental turbulence increases. This is particularly clear with reference to firms' overall performance. These findings are of theoretical and practical relevance for the study of the effects of public policies as regards their nature and the policy design mix
The RNA recognition motif protein RBM11 is a novel tissue-specific splicing regulator.
No full textMammalian tissues display a remarkable complexity of splicing patterns. Nevertheless, only few examples of tissue-specific splicing regulators are known. Herein, we characterize a novel splicing regulator named RBM11, which contains an RNA Recognition Motif (RRM) at the amino terminus and a region lacking known homology at the carboxyl terminus. RBM11 is selectively expressed in brain, cerebellum and testis, and to a lower extent in kidney. RBM11 mRNA levels fluctuate in a developmentally regulated manner, peaking perinatally in brain and cerebellum, and at puberty in testis, in concomitance with differentiation events occurring in neurons and germ cells. Deletion analysis indicated that the RRM of RBM11 is required for RNA binding, whereas the carboxyl terminal region permits nuclear localization and homodimerization. RBM11 is localized in the nucleoplasm and enriched in SRSF2-containing splicing speckles. Transcription inhibition/release experiments and exposure of cells to stress revealed a dynamic movement of RBM11 between nucleoplasm and speckles, suggesting that its localization is affected by the transcriptional status of the cell. Splicing assays revealed a role for RBM11 in the modulation of alternative splicing. In particular, RBM11 affected the choice of alternative 5' splice sites in BCL-X by binding to specific sequences in exon 2 and antagonizing the SR protein SRSF1. Thus, our findings identify RBM11 as a novel tissue-specific splicing factor with potential implication in the regulation of alternative splicing during neuron and germ cell differentiation
Città e territori del PNRR. Casi e approfondimenti
No full textQuesto volume raccoglie i Background Papers del IX Rapporto di Urban@it, dedicato all’attuazione del Piano Nazionale di Ripresa e Resilienza. Le riflessioni che sono state sistematizzate nel Rapporto, e delle quali i contributi raccolti sono parte integrante, hanno carattere parziale e temporaneo perché nascono dall’osservazione in itinere di un processo i cui risultati potranno essere effettivamente ponderati dopo la scadenza del 2026. Più precisamente, i contributi raccolti in questo volume si riferiscono al periodo dall’inizio del 2022 fino al mese di luglio
2023, attestandosi alle soglie della revisione del Piano richiesta nel mese di agosto 2023 dal governo italiano e accolta soltanto in parte dall’Europa. Si tratta di una osservazione che fotografa successi e criticità del primo periodo di attuazione del Pnrr, con particolare riferimento ad alcune città che costituiscono luoghi di osservazione privilegiata per gli aderenti alla rete Urban@it
Osservare e valutare nel PNRR. Flussi informativi e criteri valutativi per città e territori nel nuovo ciclo di politiche pubbliche.
No full textQuesto contributo analizza i primi risultati di un'indagine avviata nel gennaio
2021 sul Piano Nazionale di Ripresa e Resilienza (PNRR). Si esplorano i flussi
informativi e le valutazioni nel processo di sviluppo del piano, con particolare
attenzione alla governance. L'autore rileva criticità nella struttura del PNRR,
sottolineando la mancanza di un quadro di coerenza spaziale e una governance
che limita il coinvolgimento degli enti territoriali. La revisione del PNRR nel
luglio 2023 presenta elementi contraddittori, sollevando domande sulla
coerenza e sull'allocazione delle risorse. In conclusione, l'articolo evidenzia la
necessità di valutazioni approfondite e del coinvolgimento degli enti territoriali
per garantire il successo del piano.This contribution analyzes the first results of a survey launched in January 2021
on the National Recovery and Resilience Plan (PNRR). Information flows and
assessments are explored in the plan development process, with a particular
focus on governance. The author notes criticality in the structure of the PNRR,
stressing the lack of a framework of spatial coherence and governance that
limits the involvement of local and regional authorities. The revision of the
PNRR in July 2023 presents contradictory elements, raising questions about
consistency and resource allocation. In conclusion, the article highlights the
need for in-depth assessments and the involvement of local and regional
authorities to ensure the success of the plan
Il Pnrr e la nuova mobilità di Bologna
No full textIl contributo descrive le iniziative di Bologna riguardanti il Piano Nazionale di Ripresa e Resilienza (PNRR) in merito alla mobilità urbana. Il testo delinea strategie di investimento, in particolare nei progetti di mobilità sostenibile, come linee tramviarie, piste ciclabili e miglioramenti del trasporto pubblico. La città mira a cambiare le preferenze di trasporto modale, enfatizzando il trasporto pubblico, le biciclette e i percorsi pedonali, mentre illustra le sfide nel coordinamento dei progetti, nelle fonti di finanziamento e nei tempi di implementazione
La terapia termale sulfurea nella cura dell' otite media secretiva dell' infanzia. Nostra esperienza.
No full textIn vitro neurogenesis: development and functional implications of iPSC technology
No full textNeurogenesis is the developmental process regulating cell proliferation of neural stem cells, determining their differentiation into glial and neuronal cells, and orchestrating their organization into finely regulated functional networks. Can this complex process be recapitulated in vitro using induced pluripotent stem cell (iPSC) technology? Can neurodevelopmental and neurodegenerative diseases be modeled using iPSCs? What is the potential of iPSC technology in neurobiology? What are the recent advances in the field of neurological diseases? Since the applications of iPSCs in neurobiology are based on the capacity to regulate in vitro differentiation of human iPSCs into different neuronal subtypes and glial cells, and the possibility of obtaining iPSC-derived neurons and glial cells is based on and hindered by our poor understanding of human embryonic development, we reviewed current knowledge on in vitro neural differentiation from a developmental and cellular biology perspective. We highlight the importance to further advance our understanding on the mechanisms controlling in vivo neurogenesis in order to efficiently guide neurogenesis in vitro for cell modeling and therapeutical applications of iPSCs technology
IX RAPPORTO SULLE CITTA'. Le città e i territori del PNRR. Attori, Processi, Politiche
No full textitalian
Mitochondrial abnormalities in induced pluripotent stem cells-derived motor neurons from patients with riboflavin transporter deficiency
No full textRiboflavin transporter deficiency (RTD) is a childhood-onset neurodegenerative disorder characterized by sensorineural deafness and motor neuron degeneration. Since riboflavin plays key functions in biological oxidation-reduction reactions, energy metabolism pathways involving flavoproteins are affected in RTD. We recently generated induced pluripotent stem cell (iPSC) lines from affected individuals as an in vitro model of the disease and documented mitochondrial impairment in these cells, dramatically impacting cell redox status. This work extends our study to motor neurons (MNs), i.e., the cell type most affected in patients with RTD. Altered intracellular distribution of mitochondria was detected by confocal microscopic analysis (following immunofluorescence for superoxide dismutase 2 (SOD2), as a dual mitochondrial and antioxidant marker), and βIII-Tubulin, as a neuronal marker. We demonstrate significantly lower SOD2 levels in RTD MNs, as compared to their healthy counterparts. Mitochondrial ultrastructural abnormalities were also assessed by focused ion beam/scanning electron microscopy. Moreover, we investigated the effects of combination treatment using riboflavin and N-acetylcysteine, which is a widely employed antioxidant. Overall, our findings further support the potential of patient-specific RTD models and provide evidence of mitochondrial alterations in RTD-related iPSC-derived MNs—emphasizing oxidative stress involvement in this rare disease. We also provide new clues for possible therapeutic strategies aimed at correcting mitochondrial defects, based on the use of antioxidants
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