524 research outputs found
The functional role of EZH2 inactivation in the pathogenesis of T-cell acute lymphoblastic leukemia
No full textT-cell acute lymphoblastic leukemia (T-ALL) is a hematological malignancy, characterized by frequent loss-of-function mutations in EZH2 gene. EZH2 is a histone-lysine N- methyltransferase enzyme and in mouse, its inactivation leads to the initiation and progression of T-cell leukemia. Here, we characterized the in vitro phenotypes associated with a long-term expansion of human CD34+ hematopoietic stem and progenitor cells (HSPCs) in response to CRISPR/Cas9-mediated inactivation of EZH2 alone or in combination with the constitutive expression of known T-ALL oncogenes after lentiviral transduction. It is known that early T cell leukemias with inactivating EZH2 mutations are closely associated with the upregulation of HOXA9 and LYL1 oncogenes. In our in vitro model EZH2 knockout in LYL1 or HOXA9- transduced HSPCs, expanded in vitro up to 31 days, exhibited an increased leukemia clonogenic capacity and promoted an enrichment of early progenitor T cells at the double negative 1 (DN1) development stage. Furthermore, EZH2-null LYL1 and EZH2-null HOXA9- transduced cells demonstrated high penetrance leukemia in vivo experiments. RNA-seq data from ETP-ALL-like LOUCY cells revealed that EZH2 knockout overexpressing HOXA9 or LYL1 resulted in a gene expression signature of immature T-ALL, stem/immature immunophenotypic stage, increased replication status, and enhanced dependency on the inflammation response. Lastly, RNA-seq data analysis from a large pediatric cohort of T-ALLs revealed that patients with Loss of Function (LoF) mutations in EZH2 gene shared a stem cell- associated transcriptional program and were statistically enriched for the immature HOXA cluster and ETP subtype in human T-ALL leukemia
In the shadow of the church: the building of mosques in early medieval Syria
No full textIn his book In the Shadow of the Church: The Building of Mosques in Early Medieval Syria Mattia Guidetti examines the establishment of Muslim religious architecture within the Christian context in which it first appeared in the Syrian region, contributing to the debate on the transformation of late antique society to a Muslim one. He scrutinizes the slow process of conversion to Islam of the most important town centers by looking at religious places of both communities between the seventh and the eleventh century. The author assesses the relevancy of churches by analyzing the location of mosques and by researching phenomena of transfer of marble material from churches to mosques
Internet of Things as a Means to Improve Agricultural Sustainability
No full textThe paper presents a technological platform that favours the introduction of sustainable processes in agriculture. The platform is based on an extensive use of Wireless Sensor Networks, where low power nodes are combined with multimedia devices to integrate meteorological measurements with high definition pictures. Two different solutions are discussed: a short range, multi hop architecture and its long range, single hop variation. Experimental results demonstrate the performance of the proposed solutions, which appears particularly suitable to continuously and pervasively monitor vineyards, favouring a measurable reduction in the use of pesticides
RESVERATROL-INDUCED AUTOPHAGY CONTRIBUTES TO THE INHIBITION OF EPSTEIN BARR VIRUS REPLICATION IN BURKITT’S LYMPHOMA CELLS
No full textRESVERATROL-INDUCED AUTOPHAGY CONTRIBUTES TO THE INHIBITION OF EPSTEIN BARR VIRUS REPLICATION IN BURKITT’S LYMPHOMA CELLS
De Leo Alessandra (a), Colavita Francesca (a), Arena Giuseppe (b), Mattia Elena (a)
(a) Dip. di Scienze di Sanità Pubblica e Malattie Infettive “Sanarelli”, Univ. di Roma “Sapienza”
(b) Casa Sollievo della Sofferenza Hospital, CSS-Mendel Institute, Roma
Presenting author: De Leo Alessandra, [email protected]
We have previously examined the antiviral activity of resveratrol on the replication of Epstein Barr Virus (EBV), the etiologic agent of infectious mononucleosis and associated with several types of malignancies of epithelial and lymphoid origin.
In a cellular context that allows in vitro EBV activation and lytic cycle progression through mechanisms closely resembling those that in vivo initiate and enable productive infection, we found that RV inhibited EBV lytic genes expression and the production of viral particles in a dose-dependent manner
A preliminary synthesis of a light and compact wearable cable-driven parallel robot for wrist joint rehabilitation
No full textRobot-assisted rehabilitation has proven to be effective in clinical practice, not only because of its ability to perform repetitive and intensive rehabilitation therapy but also because its rehabilitation outcome is not limited by the lack of experienced therapists or their fatigue due to long and repetitive rehabilitation sessions. Several robotic solutions have been proposed over time, but rarely have these solutions been successful, in particular due to the excessive bulk, discomfort, and joints alignment of the robotic solution. This paper lays the foundation for a preliminary synthesis of a wearable cable-driven parallel robot for wrist joint rehabilitation. Specifically, the synthesis focuses on defining an ad hoc geometric efficiency index to maximise the effective force required to achieve flexion-extension, radial-ulnar deviation and pronation-supination movements with the lowest possible cable tension. To consider lightness and wearability, the results of the synthesis are weighted to obtain a light and compact system that will be developed
Resistenza all'effetto profibrinolitico della proteina C attivata: una nuova causa di ipercoagulabilità nei pazienti talassemici
No full textUse of balloons and blimps to improve coverage range in Low Power Wireless Wide Area Networks
No full textThe availability of devices suitable to construct low power wireless wide area networks, offers the possibility to set up simple infrastructures for data sensing in a larger region, using simple single hop wireless sensor networks. Avoiding multi-hop routing schemes, allows to Save energy and reduces the probability of interruptions and the deployment cost. We have recently applied this solution in a hilly environment, to collect meteorological data from crops. In such an environment, when unlicensed frequencies are used, an elevated access point allows direct line-of-sight between devices. To avoid the construction of expensive towers, one possible solution is to lift the access points with balloons, blimps and kites, reaching up to 350 feet of elevation. Preliminary results are presented in the paper, together with a comparison among the technological solutions adopted for the multi and single hop cases
Anomalie cellulari e plasmatiche della coagulazione e della fibrinolisi dei linfomi ed effetto della terapia
No full textCellular and plasmatic anomalies of the coagulation system and fibrinolysis in children with lymphomas and influence of cytostatic treatment
No full textIncreased mononuclear cell tissue factor and type-2 plasminogen activator inhibitor and reduced plasma fibrinolytic capacity in children with lymphoma
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