4,715 research outputs found

    Cytosolic arylamine N-acetyltransferase (NAT) deficiency in the dog and other canids due to an absence of NAT genes

    No full text
    The purpose of this study was to determine the molecular basis in the dog for an unusual and absolute deficiency in the activity of cytosolic N-acetyltransferase (NAT), an enzyme important for the metabolism of arylamine and hydrazine compounds. NAT activity towards two NAT substrates, p-aminobenzoic acid and sulfamethazine, was undetectable in dog liver cytosol, despite substrate concentrations ranging from 10 microM to 4 mM and a wide range of incubation times. Similarly, no protein immunoreactive to NAT antibody was evident on western blot analysis of canine liver cytosol. Southern blot analysis of genomic DNA from a total of twenty-five purebred and mixed bred dogs, and eight wild canids, probed with a full-length human NAT2 cDNA, suggested an absence of NAT sequences in all canids. Polymerase chain reaction amplification of genomic DNA using degenerate primers designed to mammalian NAT1 and NAT2 consensus sequences generated products of the expected size in human, mouse, rabbit, and cat DNA, but no NAT products in any dog or wild canids. These results support the conclusion that cytosolic NAT deficiency in the domestic dog is due to a complete absence of NAT genes, and that this defect is shared by other canids.LR: 20061115; PUBM: Print; JID: 0101032; 150-13-0 (4-Aminobenzoic Acid); 57-68-1 (Sulfamethazine); 9007-49-2 (DNA); EC 2.3.1.5 (Arylamine N-Acetyltransferase); ppublishSource type: Electronic(1

    Strengthening Uganda's policy environment for investing in university development

    No full text
    The authors examine the policy environment for investment in university development in Uganda, with special attention to the needs of Makerere University. They present data on the structure and financing of higher education, which gets a high priority in government educational spending. A second public university and new private universities have been established since 1986, but Makerere accounts for most university enrollment and government spending on higher education and it trains most of the country's high-level professional and technical manpower. Its revitalization after many years of neglect is central to government and donor plans for investment in human resource development. The authors emphasize how continuing austerity affects staff retention and staff engagement in academic work, as well as the quality of programs Makerere offers. They present a strategy for university development that involves establishing policy structures to: guide and coordinate investments in higher education as a whole; facilitate the expansion of higher education and the development of diploma-granting institutions to accommodate increasing social demand; and promote cost-saving and revenue-generating activities in the public universities - which would require giving them more autonomy in matters affecting their cost structure and budgeting. Among specific actions they recommend: making better use of public university assets by developing night courses, part-time degree and non-degree programs, and contract training and other income-generating activities; investigating possibilities for better use of university farms and other properties; making more use of existing capacity in public institutions and increasing the capacity of the newly established private universities; strengthening secondary education in science subjects and encouraging more women to study science and technology; coordinating future donor investments so they address the broad needs of Makerere and other universities; and raising incomes of academic and nonacademic university staff members.Teaching and Learning,Curriculum&Instruction,Gender and Education,Tertiary Education,Primary Education

    Greek Author

    No full text
    Greek Author. Naples Nat\u27l. Arch. Mus. XII.13.6https://digitalcommons.acu.edu/ferguson_photos/1276/thumbnail.jp

    On the Author Correction: Magnetic field screening in hydrogen-rich high-temperature superconductors , Nat Commun 14, 5322 (2023)

    No full text
    I analyze the implications of the recently published Author Correction (Nat Commun 14, 5322 (2023)) to a paper by Eremets and coauthors reporting magnetization measurements on hydrides under high pressure (Nat Commun 13, 3194 (2022)) to the understanding of the validity and reproducibility of the published data. This paper is a compilation of several different papers already published or to be published in the scientific literature.The underlying measured data referred to in this article as unavailable have recently been made available at https://osf.io/7wqxb/ August 9, 202

    Rivierbocht model, doseerinstallatie nat zand

    No full text
    Begonnen is voor het rivierbochtmodel een doseerinstallatie te ont-vrikkelen, die net zo nauwkeurig als het doseerapparaat voor droog zand, nat zand doseert. De grote voordelen hierbij zijn: - de mogelijkheid continu dag en nacht te doseren, - minder arbeidsintensief (niet drogen en vullen). Hiervoor is met de heer Van der Brugge en de heer Groeneveld een bezoek gebracht aan het W.L. in de Voorst, om daar te kijken hoe op de rechte goot het doseerprobleem is opgelost. Aan de hand van dit bezoek is verder gedacht over wat in ons geval een goed systeem zou zijn.oHydraulic EngineeringCivil Engineering and Geoscience

    Structural changes in metals consumption

    No full text
    For 15 years the metals market has been characterized by slow growth - in some cases, even decline - in consumption. To test the proposition that structural changes in demand were the main cause of the slowdown, the author - drawing on U.S. data - uses an extended metals demand model that recognizes energy, labor, capital, and other materials as major inputs. The traditional model explains metals consumption in terms only of output and the prices of metal and its substitutes. It is inadequate to address the issue of structural change because it ignores other factors of production, such as energy, which have experienced dramatic changes. With the extended model, the null hypothesis of no structural change cannot be rejected for most metals. With the conventional model, the null hypothesis of no structural change is strongly rejected. Results with the extended model show that the downturn can be explained mostly by changes in the input variables, particularly such nonmetal inputs as capital and energy, which are much more important cost items than metals and have undergone drastic cost changes over the period.Environmental Economics&Policies,Economic Theory&Research,Montreal Protocol,Mining&Extractive Industry (Non-Energy),Primary Metals

    CITIZEN SECURITY – The Role of NGOs and Broader Civil Society in Ceasefire Monitoring: Lessons from Mindanao

    No full text
    This article examines the role of NGOs and broader civil society in laying the ground for sustainable peace and development by maintaining and promoting early security and stability through the monitoring of ceasefire agreements. Central activities include preventing protagonists from engaging in violence, monitoring the peace process, documenting violations and promoting dialogue. The article draws lessons from an analysis of the ‘Bantay Ceasefire’ (Ceasefire Watch), a closely networked grassroots movement of NGO and other civil society actors which was formed to establish community security through monitoring a ceasefire agreement between the Moro Islamic Liberation Front (MILF) and the Armed Forces of the Philippines (AFP) in Mindanao, the second largest island of the Philippines. The article adds to the growing literature on the strategic role of NGOs and wider civil society in enabling citizen security as the missing link at the nexus of peace, security and development.Submitted by Kari Schmidt ([email protected]) on 2009-05-18T16:10:58Z No. of bitstreams: 1 Colletta_v2n3.pdf: 816978 bytes, checksum: e4ad75aa5a0dc1dfc9f3fcdcc826535d (MD5)Made available in DSpace on 2009-05-18T16:10:58Z (GMT). No. of bitstreams: 1 Colletta_v2n3.pdf: 816978 bytes, checksum: e4ad75aa5a0dc1dfc9f3fcdcc826535d (MD5) Previous issue date: 200

    The 'Nat Turner' Controversy

    No full text
    In the late sixties, William Styron, who had acquired considerable reputation with his first three novels, published "The Confessions of Nat Turner", a fictionalized account of a slave rebellion in Virginia in 1831. The author himself, as well as influential white critics, thought that the book was highly sympathetic to Nat Turner, the black rebel leader, and expected a positive response from the black community. However, a number of black Writers attacked Styron for what they saw as profoundly racíst attitudes. This paper examines the problematic aspects of the novel and the main issues of this controversy as generally illustrative of the difficulties involved in bridging cultural and historical misunderstandings and, in its particularities, as characteristic of America in the 196os

    NAT traversal techniques for MediaSense open source platform

    No full text
    This thesis project concerns NAT traversal techniques and their application to P2P networking with regard to MediaSense platform. Since MediaSense open source platform, developed by Mid Sweden University, utilizes the benefits of P2P networking, it also suffers from the drawbacks provided by NAT. The issue of NAT traversal is not trivial due to the fact that the behavior of NAT devices is not standardized and vendors are free to provide their own implementations. The common knowledge is, that at least four main types of NATs exist, differing in the filtering and mapping algorithms employed. NAT traversal techniques vary accordingly. No single technique can handle all the cases. Most of the techniques can handle up to three main types of NAT. The last type is usually used in large corporate networks and is called the Symmetric NAT. The most viable, and basically the only available technique for its traversal, is data relaying. This thesis builds a NAT traversal module for the MediaSense platform. The main purpose of this module is to provide seamless NAT traversal capabilities to the platform. The module does this in several steps: UPnP enabled device discovery, NAT type determination and data relaying via the proxy. Firstly the module attempts to discover the presence of a UPnP enabled Internet Gateway Device on the network. If such a device is present on the network, a port mapping can be created, making the node located behind NAT accessible from the public Internet. If a UPnP enabled device was not found, the module will try to determine the type of NAT used. Based on the type of NAT used, the module can transit to either the proxy mode or request assistance of the STUN server to keep the created mapping alive. The resulting chapters provide the reader with the output produced by each step, conclusions the author has made while working on this project and some general ideas on future work within the subject
    corecore