1,721,001 research outputs found
Characterization of transcription factors: a pipeline. Experimental evidence on the transcription factor FD in Arabidopsis thaliana
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New insights on the bZIP transcription factor FD promoting flowering in Arabidopsis thaliana
No full textSoPPIs, Sequencing of Protein-Protein Interactions: a powerful solution to a remarkable problem
No full textOil rich seeds of Jatropha curcas as a renewable source of biodiesel: genotyping clones of cultivated varieties and cloning genes for fatty acid biosynthesis
No full textJatropha is becoming a popular non-food oleaginous crop in several developing countries for its proposed value for the production of bioenergy. Despite the great potentials of its oil-rich seeds as renewable source of biofuel and the broad interest toward large-scale plantations, relatively little is known with respect to population genetics and functional genomics. We have performed SSR marker analysis to gain new insights on the genetic diversity structure of 123 multiple accessions, and the genetic relationships among commercial clones and locally dominant ecotypes. Based on our findings, seeds commercialized worldwide seem to include a few closely related genotypes that show a very low differentiation among cultivars. In addition, we have cloned and characterized 12 genes that control key steps of FA biosynthesis for a better understanding of the polymorphism information content of their allelic variants. The sequencing of these genes was pursued for discovering a set of discriminant SNPs and for haplotyping a core collection of accessions. Gene expression levels were also determined in two distinct developmentally staged seeds by means of quantitative Real-Time PCR assays. The genetical and functional analysis of seed-specific fatty acid desaturases (FAD/SAD) is the first step for selecting or generating high-oleate J. curcas genotypes, and also for breeding oil-rich J. curcas cultivars suitable for biofuel production
Gibberellic acid signaling is required for ambient temperature-mediated induction of flowering in Arabidopsis thaliana
No full textDistinct molecular mechanisms integrate changes in ambient temperature into the genetic pathways that govern flowering time in Arabidopsis thaliana. Temperature-dependent eviction of the histone variant H2A.Z from nucleosomes has been suggested to facilitate the expression of FT by PIF4 at elevated ambient temperatures. Here we show that, in addition to PIF4, PIF3 and PIF5, but not PIF1 and PIF6, can promote flowering when expressed specifically in phloem companion cells (PCC), where they can induce FT and its close paralog, TSF. However, despite their strong potential to promote flowering, genetic analyses suggest that the PIF genes seem to have only a minor role in adjusting flowering in response to photoperiod or high ambient temperature. In addition, loss of PIF function only partially suppressed the early flowering pheno- type and FT expression of the arp6 mutant, which is defective in H2A.Z deposition. In contrast, the chemical inhibition of gibberellic acid (GA) biosynthesis resulted in a strong attenuation of early flowering and FT expression in arp6. Furthermore, GA was able to induce flowering at low temperature (15°C) independently of FT, TSF, and the PIF genes, probably directly at the shoot apical meristem. Together, our results suggest that the timing of the floral transition in response to ambient temperature is more complex than previously thought and that GA signaling might play a crucial role in this process
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