1,593 research outputs found
Luminescence Immunoassays
No full textThis article is an update version of G. Gübitz, M.G. Schmid, IMMUNOASSAYS, TECHNIQUES | Luminescence Immunoassays, Editor(s): Paul Worsfold, Alan
Townshend, Colin Poole, Encyclopedia of Analytical Science (Second Edition), Elsevier, 2005, Pages 352–360, ISBN 9780123693976.Immunoassays (IAs) are analytical methods based on the specific recognition between the antigen (Ag), which molecular weight
can vary in a wide range, and the variable regions of antibody (Ab), a Y shaped macromolecule composed by four polypeptide
chains. IAs are applicable without analytes enrichment, purification, or pretreatment having great advantages over standard
methods. Immunoassays relate to medicine, food quality control, forensic veterinary, and environmental analysis.
A detectable label conjugated on one or both of the immunoreagents (IRs) reveals the recognition between Ag and Ab. The
evolution of IAs corresponded to the evolution of the labeling technology and the optical detection is the simplest way to evaluate
the analytical signal
Developing a partnership of indigenous peoples, conservationists, and land use planners in Latin America
No full textIllustrating from a rich body of case material, Poole's report reflects a shift away from the traditional view - represented by certain national parks and similar protected areas - that indigenous peoples be allowed to occupy and use an area's resources following rules set by conservationists. Under the new paradigm that is developing, indigenous peoples are seen as an integral part of protected area planning through agreements worked out in partnership with conservation authorities. An example of this new approach is the role that indigenous peoples are playing in the design of biosphere reserves. Poole suggests that the Bank and other development organizations pay more attention to vernacular economies - economies based on local resources, used either for subsistence or as a source of revenue. He also recommends more research into economics and resource implications of these local activities to harvest wild resources, especially in environmentally delicate areas such as tropical rainforests.Environmental Management,Tourism and Ecotourism,Water Conservation,Natural Resources Management,Wetlands
Proline transport and stress tolerance of ammonia-insensitive mutants of the PUT4-encoded proline-specific permease in yeast
No full textThe imino amino acid, proline, has roles in both cellular nutrition and response to stress. Proline uptake in Saccharomyces cerevisiae is largely mediated by a high affinity, specific permease, Put4p, and a low affinity general amino acid permease, Gap1p. Both are subject to nitrogen catabolite repression (NCR) and nitrogen catabolite inactivation (NCI). In order for proline to be fully exploited, its transport must be derepressed, as occurs upon depletion of preferred nitrogen sources, and molecular oxygen must be present to allow the first step of catabolism via proline oxidase. This study focuses on the isolation of variants of Put4p, which are insensitive to repression by a preferred nitrogen source (ammonia) and their subsequent effect on proline transport and stress tolerance. Specific amino acid residues in the carboxy-terminal region of Put4p were targeted by site-directed mutagenesis. Substitution at Serine(605), a potential phosphorylation target, led to the amelioration of ammonia-induced down-regulation of Put4p. When combined with a promoter mutation (-160), the S(605)A mutation resulted in increased proline uptake and accumulation. This increase in proline accumulation was associated with increased cell viability in conditions of high temperature and osmotic stress raising possible benefits in industrial fermentation applications.Kate Poole, Michelle E. Walker, Tristan Warren, Jennie Gardner, Colin McBryde, Miguel de Barros Lopes and Vladimir Jiranekhttp://www.ncbi.nlm.nih.gov/pubmed/2011860
Theoretical principles of capillary electromigration methods
No full textDuring the past 50 years, electrophoretic separations and analyses in capillaries and microchannels emerged as techniques which provide high-resolution separations with on-column and/or end-column sample detection while requiring only small amounts of sample and reagents. Techniques developed include capillary zone electrophoresis, capillary isotachophoresis, capillary isoelectric focusing, capillary gel electrophoresis, affinity capillary electrophoresis, electrokinetic capillary chromatography and capillary electrochromatography. This chapter provides the theoretical principles of capillary electromigration methods, namely short descriptions of electrokinetic phenomena, efficiency of analyte transport, the concept of mobility, dispersion and focusing of analytes, the regulating principle, the occurrence of system peaks, dynamic computer simulations of capillary electrophoresis and other computer models to predict transport and separation in capillary electrophoresis
Steam Engine Sir Colin Campbell at Glossop Fair
No full textHibbert's Burrell steam traction engine No.2806 'Sir Colin Campbell' photographed at Glossop Fair, 6 May 1937. Digitisation and record funded by the Pilgrim Trust
Water analysis/Industrial Effluents
No full textIndustrial effluents result from various types of industrial processes and disposal practices, and may
contain pollutants at levels that could affect the quality of receiving waters, as well as the aquatic ecosystem. The emission of industrial pollutants in liquid effluents has to comply with stringent regulatory requirements and guidelines, in which chemicals listed should not exceed a given concentration. On the other hand, a chemical company may release a large number of different chemicals, which are not considered by regulatory requirements and in many cases are unknown. These compounds may be the final products, precursors, or intermediates of the process, or impurities and byproducts. One of the distinguishing characteristics of effluents of industrial origin, as compared to municipal wastewaters, is that often they may contain a mixture of different and very toxic substances. Approved analytical methods exists for compliance monitoring of conventional pollutants in industrial effluents; however, because of the complexity of the sample matrix, several analytical methods are required to determine polar and nonpolar organic compounds and new emerging pollutants that may impact water quality. As a consequence, modifications in instrumentation, sampling, and sample preparation techniques have become essential to comply with the regulatory water standards, as well as to achieve a faster speed of analysis
Map of the central section of British Columbia : shewing the country served by the Grand Trunk Pacific Railway
No full textScale [ca. 1:6,400] (W 133 00'--W 118 00'/N 56 00'--N 53 00'). Relief shown by hachures. Across top of map: 2,000,000 acres agricultural land
Long-term morphological model of Poole Harbour
No full textThe study focuses on the impact of human interventions on the natural behaviour of estuaries in response to sea-level rise. In particular it focuses on the affect of managed realignment on the critical sea level rise (SLRcrit) for the Poole Harbour. Numerical model ASMITA (Aggregated Scale Morphological Interaction between a Tidal inlet and the Adjacent coast, Stive and Wang, 1996) is used to study the response of the system to the external forcing (both natural and anthropogenic). ASMITA model schematises the tidal-inlet system and characterises each model element by a single variable volume. Volumetric data of the Poole harbour inlet elements obtained through the digitisation of the historical admiralty charts was used to calibrate the model. The model assumes that the each inlet elements tends towards an equilibrium volume which can be defined using the empirical equations. These equilibrium equations consist of equilibrium parameters that depend on the regional properties like hydrodynamics and topography of the Poole harbour. Once the model is calibrated, simulations were performed with various sea level rise scenarios (historic and future SLR) to observe the natural behaviour of the Poole harbour. Managed realignment is being introduced in the model and a range of values of critical sea-level rise was obtained and compared with the predicted sea-level rise rate given in the literature for the next century. Results suggest that the “hold the line” management scenario (current situation) would not result in the complete loss of inter-tidal flats and the coastal flooding is unlikely to occur over the next century, as a result of critical sea-level rise values exceeds the predicted rate. However research suggests, introducing realignment (coastline retreat management scenario) reduces SLRcrit values for the estuary; thus making the estuary more sensitive to the predicted future SLR rates.CoMEMHydraulic EngineeringCivil Engineering and Geoscience
One Hundred Years of Showmanship: Poole's 1837-1937
No full textBooklet describes the Poole family's history as creators of 'panoramas, dioramas, myrioramas, theatres, variety halls, and cinemas.' Illustrations of some of the scenes portrayed, and an explanation of how some of the spectacular effects were produced. Second part consists of a Jim Poole autograph postcard (dated Feburary 1985; addressed to Bill Douglas). Jim Poole was son of the author. Page number(s) as given:
files ending 01_001, 01_001_2, 01_010, 01_010_2, 02, 02_2 are not included in pagination, files ending 002-009_2 are pp.1-1
Coat Cooke & Joe Poole | Coat Cooke & Rainer Wiens: Reviews
No full textCoat Cooke album reviews by Randy Raine-Reusch. Coat Cooke (sax); Joe Poole (drums); Rainer Wiens (guitar)
- …
