8,984 research outputs found
Pellicle Biofilm Formation in Burkholderia cenocepacia J2315 is Epigenetically Regulated through WspH, a Hybrid Two-Component System Kinase-Response Regulator
Get PDFThe chemosensory signal transduction system Wsp regulates biofilm formation and related phenotypes by influencing cyclic-di-GMP (c-di-GMP) levels in bacterial cells. This is typically achieved by activation of the diguanylate cyclase WspR, through phosphorylation of its phosphoreceiver domain. The Wsp system of Burkholderia cenocepacia J2315 is in one operon with the hybrid response regulator/histidine kinase wspH, but lacks the diguanylate cyclase wspR which is located in a different operon. The expression of wspH, the first gene in the B. cenocepacia Wsp operon as well as pellicle biofilm formation are epigenetically regulated in B. cenocepacia J2315. To investigate whether WspH regulates pellicle biofilm formation, several mutants with altered expression of wspH were constructed. Mutants with increased expression of wspH showed accelerated pellicle biofilm formation, reduced swimming motility and increased c-di-GMP levels. This was independent of WspR phosphorylation, showing that WspR is not the cognate response receiver for histidine kinase WspH. IMPORTANCE Biofilms are surface-attached or suspended aggregates of cells, that are problematic in the context of bacterial infections, as they provide protection from antibiotic treatment. Burkholderia cenocepacia can colonize the lung of immunocompromised patients and forms biofilms that increase its recalcitrance to antibiotic treatment. Pellicles are biofilms which form at an air-liquid interface to take advantage of the higher oxygen concentrations in this environment. How quickly pellicles are formed is crucial for the fitness of obligate aerobic bacteria such as B. cenocepacia. Cyclic-di-GMP (c-di-GMP) levels determine the transition between planktonic and biofilm lifestyle, and WspH controls c-di-GMP production. WspH is therefore important for the fitness of B. cenocepacia in environments with gradients in oxygen concentration, such as the human lung
Michael Rodriguez interviews author Tom Springer
No full textAuthor Tom Springer is interviewed about his writing career and his newest book "Looking for hickories". Springer talks about his career following after earning an Environmental Journalism degree from Michigan State University. He calls his genre "creative non-fiction" and explains how he weaves his memories into his books about life in rural and wild Michigan. Part of the Michigan State University Libraries' Michigan Writers Series. Springer is interviewed by Librarian Michael Rodriguez
Performing the archive: following in the footsteps
Get PDFUsing documentation of Mike Pearson's performance 'Bubbling Tom', Deirdre Heddon attempts to step into his shoes and re-perform it
The future of biofilm research – report on the ‘2019 Biofilm Bash’
No full textIn May 2019, 29 scientists with expertise in various subdisciplines of biofilm research got together in Leavenworth (Seattle, WA, USA) at an event designated as the 2019 ‘Biofilm Bash’. The goal of this informal two-day meeting was first to identify gaps in our knowledge, and then to come up with ways how the biofilm community can fill these gaps. The meeting was organized around six questions that covered the most important items brought forward by the organizers and participants. The outcome of these discussions is summarized in the present paper. We are aware that these views represent a small subset of our field, and that inevitably we will have inadvertently overlooked important developing research areas and ideas but we are nevertheless hopeful that this report will stimulate discussions and help create new ways how we can advance our field
CRE Author Tom Franklin
No full textCommon Reading Experience author and UM creative writing instructor Tom Franklin talks about his novel, Crooked Letter, Crooked Letter. Video by Mary Stanton.https://egrove.olemiss.edu/umvideo/1334/thumbnail.jp
Tom Kubancik
No full textTom is the Vice President of Advanced Programs at Applied Defense Solutions (ADS).
Tom’s entire career has been focused on advanced technology with over 30 years in Space Systems, High Performance Computing, and Microelectronics. With a background in Operations Management, Tom has enjoyed broad success when pioneering companies in rapidly evolving markets and shaping today’s high technology landscape.
Tom is a recognized international expert in Space Situational Awareness (SSA), participating in research, development, and deployment programs since the 1980’s. At Applied Defense Solutions (ADS), Tom has led the transition away from military-only SSA, establishing a broad portfolio of research and development, commercialization, and operational support programs. ADS is a recognized leader in civilian, commercial, and government space exploration, focusing on all phases from mission analysis, operations support, and space protection. Leading the ADS Advanced Programs’ team, Tom coordinates a highly talented group of technical experts working alongside program managers, operational experts, and capture professionals. Their focus is to create and develop opportunities for ADS to apply its innovations and expertise to the most challenging space systems development tasks. His team harnesses a company-wide passion for problem-solving by leveraging a world class research portfolio with exquisite analytical capabilities and deep operational experience. ADS has constructed the most interesting mission portfolio in the industry as Tom and his team love their role in defining the next generation of safe space operations.
Tom is an active participant in NATO Science and Technology panels and activities leading to better understanding of global approaches for effective coalition and collaborative SSA. Tom is a published author on global SSA and is a frequent speaker at domestic and international conferences.
Tom has a wealth of experience with leadership positions. He is a graduate of Bowling Green University. Tom and his family live in Boulder, Colorado.https://commons.erau.edu/stm-images/1097/thumbnail.jp
Tom Lawson
No full textTom Lawson is Professor of History and Pro Vice Chancellor for Arts, Design and Social Sciences at Northumbria University. He is the author and editor of several books including Debates on the Holocaust (2010) and most recently The Last Man: a British Genocide in Tasmania (2014).https://commons.erau.edu/genocide-bios/1044/thumbnail.jp
Compliance Update with Tom Fox
No full textJoin us for lunch with Tom Fox, compliance professional, author and creator of the Compliance Podcast Network, hosting a variety of compliance related podcasts, including a succinct daily compliance tip
First person – Tom Carruthers.
No full textFirst Person is a series of interviews with the first authors of a selection of papers published in Biology Open, helping early-career researchers promote themselves alongside their papers. Tom Carruthers is first author on ‘ exTREEmaTIME: a method for incorporating uncertainty into divergence time estimates’, published in BiO. Tom conducted the research described in this article while a PhD student in Professor Robert Scotland's lab in the Department of Plant Sciences, University of Oxford. He is now a postdoc in the lab of Dr William Baker at the Royal Botanic Gardens, Kew, working on determining the extent to which large molecular phylogenies provide information about evolutionary history
- …
