6,683 research outputs found
Ki-67 is a PP1-interacting protein that organises the mitotic chromosome periphery
No full textCopyright @ 2014 Booth et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.When the nucleolus disassembles during open mitosis, many nucleolar proteins and RNAs associate with chromosomes, establishing a perichromosomal compartment coating the chromosome periphery. At present nothing is known about the function of this poorly characterised compartment. In this study, we report that the nucleolar protein Ki-67 is required for the assembly of the perichromosomal compartment in human cells. Ki-67 is a cell-cycle regulated protein phosphatase 1-binding protein that is involved in phospho-regulation of the nucleolar protein B23/nucleophosmin. Following siRNA depletion of Ki-67, NIFK, B23, nucleolin, and four novel chromosome periphery proteins all fail to associate with the periphery of human chromosomes. Correlative light and electron microscopy (CLEM) images suggest a near-complete loss of the entire perichromosomal compartment. Mitotic chromosome condensation and intrinsic structure appear normal in the absence of the perichromosomal compartment but significant differences in nucleolar reassembly and nuclear organisation are observed in post-mitotic cells
Physical dance performance: An investigation into the development of a performance technique based on the integration of certain Korean dance technique and contemporary Western styles of dance and physical theatre
No full textThis thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University, 04/10/2001.This development of a performer practice that integrates elements of traditional Korean dance technique and Western forms of physical theatre and contemporary dance is based on an approach to internal understanding and external execution. Central to the work is the concept of body energy, or Ki. This ancient Eastern term is translated into a contemporary practice that enables a performer to engage mental and physical training. Breath and the use of breath in performing are the principal means of achieving this level of engagement
Les marqueurs de prolifération en oncologie vétérinaire : applications à l'étude pronostique du mastocytome cutané canin
No full textLe mastocytome cutané représente un type tumoral dominant de la cancérologie du chien mais demeure l'une des tumeurs canines au comportement clinique les plus imprévisibles. L'auteur évalue dans une étude rétrospective portant sur 120 chiens porteurs de mastocytome cutané, la valeur pronostique de la détection immunohistochimique des marqueurs de prolifération PCNA et Ki-67. Il montre que la détermination de l'expression de l'antigène Ki-67 permet de prédire avec une grande fiabilité l'évolution clinique post-chirurgicale de ce type tumoral
Tatari, tautoko, tauawhi - Hei awhina tamariki ki te panui pukapuka: Some preliminary findings
No full textThe Tatari, Tautoko, Tauawhi reading tutoring procedures have been adapted from the procedures known as Pause, Prompt, Praise, first developed in Mangere in 1977. The first author offered the procedures as a koha at a Special Education Service hui at Poho o Rawiri in 1991. The second author took up the koha and obtained the support of kaumatua and kuia at Hairini marae Tauranga Moana, and the support of senior Maori staff of the Special Education Service National Office to produce a Maori language video and training booklet. This began an important bicultural journey through the processes of producing instructional materials and trailing and evaluating them in ways that are biculturally appropriate. This paper reports on that journey and presents some preliminary data on the implementation of Tatari, Tautoko, Tauawhi by seven tuakana - teina pairs in a bi-lingual classroom
Ki-Bot
No full textCopyright is held by the owner/author(s).This paper explores the development of a robotic toy that will be helping students in kinematic physics. Physics has always been a hard class and it is very difficult for students to understand the concepts behind each topic in physics. Ki-bot will help students not only solve kinematic word problems, but also understand the concept of kinematic word problems by testing their answers to word problems on the bot. Ki-bot will essentially bring kinematic word problems to life to help the student understand kinematic physics. The paper will explore the advantages and disadvantages of using interactive toys for learning and will also discuss the other products in the market that help students in kinematic physics. I will also discuss the design decisions, coding decisions, and some of the testing I did in the process of building and developing Ki-Bot. Ki-bot has been successful in showing the proof of concept that this will work in helping students test their answer to word problems and understand them. Additionally, I will discuss the future work that could be done to improve Ki-bot
Generiert von Kreativer KI: Erforschung Menschlicher Reaktionen auf Informationen über KI als Quelle Kreativer Inhalte
No full textArtificial intelligence (AI) can now be used to produce a wide range of creative content, such as images, stories, music or poems. Reactions to this technological advancement could have an impact on the future development and use of this technology. This thesis examines how the information that creative content has been generated by an AI influences the experience and evaluation of creative works. The focus here is on the reception of stories and images. Possible effects on different forms of experience and judgment are examined using different stimuli materials. In addition, the mechanisms underlying these effects are tested using empirical methods. Furthermore, the potential influence of various individual differences on reactions to AI artist or author information is examined.
Manuscript 1 presents the results of two experiments (N1 = 325, N2 = 489) that indicate a negative influence of AI author information on transportation into a story. Furthermore, it was shown that genre of the story can influence this effect. The negative effects of AI author information are only evident for contemporary fiction stories, but not for the science fiction genre. The eeriness experienced while reading and the willingness to share the story are not influenced by AI artist information as originally hypothesized. Openness, affinity for technology and attitude towards AI do not influence the effect of author information on transportation. Manuscript 2 examines in two experiments (N1 = 176, N2 = 381) whether a lower appreciation of supposedly AI-generated images can be attributed to the reduced agency and experience ascribed to AI compared to humans. Both experiments show a significant indirect effect of AI artist information on the appreciation of AI-generated images, although no total effect can be observed. In addition, a positive deviation from pre-set expectations is considered as a potential mediator of a positive effect of AI artist information on appreciation. However, no mediating effect becomes apparent. Manuscript 3 addresses a possible algorithmic bias in AI-generated images. Results of a content analysis of 324 AI-generated images of people working in STEM professions show that women and POC are
strongly underrepresented. Results of an experiment (N = 495) indicate that these biases are not reflected in responses to AI artist information. Rather, the information that images are AI-generated increases the perceived objectivity of some of the images. Furthermore, AI artist information causes moral outrage when the AI-generated images show older people.
In sum, the information that creative content has been generated by an AI, and not created by a human, can impact responses to these works. However, these effects can be influenced by the genre or the specific content. The reactions to AI artist or author information do not always have to reflect actual tendencies but depend on perceptions of AI.Künstliche Intelligenz (KI) kann inzwischen zur Erstellung vieler kreativer Inhalte, wie Bildern, Geschichten, Musik oder Gedichten genutzt werden. Reaktionen auf diesen technologischen Fortschritt könnten sich auf die künftige Weiterentwicklung und Nutzung dieser Technologie auswirken. Die vorliegende Arbeit untersucht wie die Information, dass kreative Inhalte durch eine KI generiert wurden, das Erleben und die Beurteilung kreativer Werke beeinflusst. Hierbei steht die Rezeption von Geschichten und Bildern im Fokus. Es werden mögliche Effekte auf unterschiedliche Formen des Erlebens und des Beurteilens anhand von unterschiedlichem Stimulusmaterial untersucht. Darüber hinaus werden diesen Effekten zugrunde liegende Wirkmechanismen mit empirischen Methoden geprüft. Außerdem wird der potentielle Einfluss verschiedener individueller Unterschiede auf die Reaktionen auf KI-Künstler- oder Autoreninformationen überprüft.
Manuskript 1 stellt die Ergebnisse zweier Experimente (N1 = 325, N2 = 489) vor, die auf einen negativen Einfluss von KI-Autoreninformationen auf die Transportation in eine gelesene Geschichte hindeuten. Des Weiteren zeigt sich, dass das Genre der Geschichte diesen Effekt beeinflussen kann. Die negativen Effekte der KI-Autoreninformationen zeigen sich nur für Contemporary Fiction Geschichten, nicht aber für Geschichten aus dem Genre Science-Fiction. Die erlebte Eeriness während des Lesens und die Bereitschaft die Geschichte zu teilen werden nicht wie erwartet von KI-Künstlerinformationen beeinflusst. Offenheit, Technikaffinität, sowie die Einstellung gegenüber KI haben keinen Einfluss auf die Wirkung der Autoreninformation auf die erlebte Transportation. Manuskript 2 (N1 = 176, N2 = 381) untersucht, ob eine geringere Wertschätzung vermeintlich KI-generierter Bilder auf die geringere Agency und Experience zurückgeführt werden kann, die KI im Vergleich zu Menschen zugeschrieben wird. In beiden Experimenten zeigt sich ein signifikanter indirekter Effekt der KI-Künstlerinformation auf die Wertschätzung KI-generierter Bilder, obwohl kein totaler Effekt beobachtet werden kann. Darüber hinaus wird eine positive Abweichung von den Erwartungen vor der Rezeption als möglicher positiver Wirkmechanismus von KI-Künstlerinformation auf die Wertschätzung untersucht. Allerdings zeigt sich hier kein mediierender Effekt. Manuskript 3 thematisiert eine mögliche algorithmische Darstellungsverzerrung in KI-generierten Bildern. Ergebnisse einer Inhaltsanalyse von 324 KI-generierten Bildern von Personen, die in MINT-Berufen arbeiten zeigt, dass Frauen und POC stark unterrepräsentiert sind. Ergebnisse eines Experiments (N = 495) deuten darauf hin, dass diese Verzerrungen nicht in den Reaktionen auf KI-Künstlerinformationen abgebildet sind. Vielmehr erhöht die Information, dass Bilder KI-generiert sind für einige der Bilder die wahrgenommene Objektivität dieser. Darüber hinaus rufen KI-Künstlerinformationen Empörung hervor, wenn die KI-generierten Bilder ältere Menschen zeigen.
Zusammenfassend lässt sich festhalten, dass die Information kreative Inhalte seien durch eine KI generiert und nicht durch einen Menschen geschaffen, zu veränderten Reaktionen auf diese Werke führen kann. Diese Effekte können allerdings durch das Genre bzw. den spezifischen Inhalten beeinflusst werden. Hierbei müssen die Reaktionen auf KI-Künstler- oder Autoreninformation nicht immer tatsächliche Tendenzen widerspiegeln, sondern sind stärker von der Wahrnehmung von KI abhängig
Free Convection Dominant Asymptotic Region in a Horizontal Tubular Reverse Osmosis
No full textAxial invariance of production performance encountered in a horizontal tubular reverse osmosis has been analyzed by assuming that the free convection is the dominant transport mechanism. The governing equations, which had been deduced from dimensional analyses used in heat transfer problem with a large Prandtl number, have been solved approximately by the integral method. The numerical computations show that the system performance in terms of concentration polarization and wall permeation velocity, even if axially constant, is better at the top of the tube than at any other circumferential positions. The effects of system parameters such as feed concentration level, operating pressure and tube radius can also be explained from the results obtained.This work was completed as part of author's M.S. thesis at the State University of New York at Buffalo in U.S.A. It is acknowledged that the author took advantage of critical comments from one of the evaluation committee in the process of revision
KI und Robotik fur das Human Brain Project
No full textByline: Ubbo Visser (1) Author Affiliation: (1) Department of Computer Science, University of Miami, Coral Gables, FL, 33146, USA Article History: Registration Date: 17/09/2014 Online Date: 02/10/2014Academi
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No full textSilent information regulator 2 (Sir2), an NAD+-dependent protein deacetylase, has been proposed to be a longevity factor that plays important roles in dietary restriction (DR)-mediated lifespan extension. In this study, we show that the Sir2’s role for DR-mediated lifespan extension depends on cAMP-PKA and casein kinase 2 (CK2) signaling in yeast. Sir2 partially represses the transcription of lifespan-associated genes, such as PMA1 (encoding an H+-ATPase) and many ribosomal protein genes, through deacetylation of Lys 16 of histone H4 in the promoter regions of these genes. This repression is relieved by Sir2 S473 phosphorylation, which is mediated by active cAMP-PKA and CK2 signaling. Moderate DR increases the replicative lifespan of wild-type yeast but has no effect on that of yeast expressing the Sir2-S473E or S473A allele, suggesting that the effect of Sir2 on DR-mediated lifespan extension is negatively regulated by S473 phosphorylation. Our results demonstrate a mechanism by which Sir2 contributes to lifespan extension.open
Constraining the progenitor companion of the nearby Type Ia SN 2011fe with a nebular spectrum at +981 d
No full textWe present an optical nebular spectrum of the nearby Type Ia supernova 2011fe, obtained 981 d after explosion. SN 2011fe exhibits little evolution since the +593 d optical spectrum, but there are several curious aspects in this new extremely late-time regime. We suggest that the persistence of the ?5800 Å feature is due to Na?I D, and that a new emission feature at ?7300 Å may be [Ca?II]. Also, we discuss whether the new emission feature at ?6400 Å might be [Fe?I] or the high-velocity hydrogen predicted by Mazzali et al. The nebular feature at 5200 Å exhibits a linear velocity evolution of ?350 km?s? 1 per 100 d from at least +220 to +980 d, but the line's shape also changes in this time, suggesting that line blending contributes to the evolution. At ?1000 d after explosion, flux from the SN has declined to a point where contribution from a luminous secondary could be detected. In this work, we make the first observational tests for a post-impact remnant star and constrain its temperature and luminosity to T ? 104?K?and?L?? 104?L?. Additionally, we do not see any evidence for narrow H ? emission in our spectrum. We conclude that observations continue to strongly exclude many single-degenerate scenarios for SN 2011fe
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