235 research outputs found
Agrobacterium delta rem
No full textAgrobacterium tumefaciens non-motile delta rem mutant<strong>Tilt Series Date:</strong> 2014-04-04</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 19500x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Clay Fuqua</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown and induced at 19C in liquid</p>
<strong>Sample Preparation:</strong> manual blot</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-20</p>delta_rem0007.mrc, Tilt Series (Pixel Size 0.56 nm), 3.3 GB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-20/rawdata/delta_rem0007.mrc"
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delta_rem0007_full.rec, Reconstruction (Pixel Size 2.24 nm), 516.7 MB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-20/3dimage_40998/delta_rem0007_full.rec"
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Agro delta_rem 28C liq
No full textapr-12-2014, confirmed virb2 by western<strong>Tilt Series Date:</strong> 2014-04-12</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> autoPatch</p>
<strong>Collaborators and Roles:</strong> clay fuqua</p>
<strong>Purification / Growth Conditions / Treatment:</strong> AB media, +acetosyringone ~24 hrs</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-12-15</p>agro_rem_28liq0015.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-12-15/rawdata/agro_rem_28liq0015.mrc"
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agro_rem_28liq0015.rec, Reconstruction (Pixel Size 1.92 nm), 1.1 GB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-12-15/3dimage_41398/agro_rem_28liq0015.rec"
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Agro delta_rem 28C liq
No full textapr-12-2014, confirmed virb2 by western<strong>Tilt Series Date:</strong> 2014-04-12</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> clay fuqua</p>
<strong>Purification / Growth Conditions / Treatment:</strong> AB media, +acetosyringone ~24 hrs</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-12-8</p>agro_rem_28liq0008.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-12-8/rawdata/agro_rem_28liq0008.mrc"
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agro_rem_28liq0008_part121_5.rec, Reconstruction (Pixel Size 1.92 nm), 534.5 MB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-12-8/3dimage_41384/agro_rem_28liq0008_part121_5.rec"
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Agro pJW58
No full textwild-type Agro with plasmid-borne VirC1-GFP translational fusion under control of the virB promoter. Kanamycin-resistant (150 to 300 ug/mL)<strong>Tilt Series Date:</strong> 2014-03-05</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Clay Fuqua</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AB medium</p>
<strong>Sample Preparation:</strong> Vitrobot</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-05-2</p>Agro_pJW580002.mrc, Tilt Series (Pixel Size 0.4 nm), 3.4 GB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-05-2/rawdata/Agro_pJW580002.mrc"
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Agro_pJW580002_part121_13.rec, Reconstruction (Pixel Size 1.6 nm), 356.4 MB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-05-2/3dimage_39678/Agro_pJW580002_part121_13.rec"
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Agrobacterium delta rem
No full textAgrobacterium tumefaciens non-motile delta rem mutant<strong>Tilt Series Date:</strong> 2014-04-04</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 19500x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Clay Fuqua</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown and induced at 19C in liquid</p>
<strong>Sample Preparation:</strong> manual blot</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-38</p>delta_rem0025.mrc, Tilt Series (Pixel Size 0.56 nm), 3.3 GB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-38/rawdata/delta_rem0025.mrc"
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delta_rem0025_part121_32.rec, Reconstruction (Pixel Size 2.24 nm), 516.7 MB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-38/3dimage_41028/delta_rem0025_part121_32.rec"
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Agrobacterium VirD4-GFP 28C
No full textInduction in liquid at 28C in AB+AS medium<strong>Tilt Series Date:</strong> 2014-03-24</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> IMOD</p>
<strong>Collaborators and Roles:</strong> Clay Fuqua</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Induction in liquid at 28C in AB+AS medium</p>
<strong>Sample Preparation:</strong> Vitrobot</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-24-6</p>VirD4_GFP_Clay20001.mrc, Tilt Series (Pixel Size 0.4 nm), 3.4 GB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-24-6/rawdata/VirD4_GFP_Clay20001.mrc"
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VirD4_GFP_Clay20001_part121_40.rec, Reconstruction (Pixel Size 0.0 nm), 356.4 MB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-24-6/3dimage_49423/VirD4_GFP_Clay20001_part121_40.rec"
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Agrobacterium delta rem
No full textAgrobacterium tumefaciens non-motile delta rem mutant<strong>Tilt Series Date:</strong> 2014-04-04</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 19500x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Clay Fuqua</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown and induced at 19C in liquid</p>
<strong>Sample Preparation:</strong> manual blot</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-17</p>delta_rem0004.mrc, Tilt Series (Pixel Size 0.56 nm), 3.3 GB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-17/rawdata/delta_rem0004.mrc"
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delta_rem0004_full.rec, Reconstruction (Pixel Size 2.24 nm), 516.7 MB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-17/3dimage_40986/delta_rem0004_full.rec"
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Agro pJW58
No full textwild-type Agro with plasmid-borne VirC1-GFP translational fusion under control of the virB promoter. Kanamycin-resistant (150 to 300 ug/mL)<strong>Tilt Series Date:</strong> 2014-03-05</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Clay Fuqua</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AB medium</p>
<strong>Sample Preparation:</strong> Vitrobot</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-05-19</p>Agro_pJW58_30004.mrc, Tilt Series (Pixel Size 0.4 nm), 3.4 GB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-05-19/rawdata/Agro_pJW58_30004.mrc"
> <i class="download icon"></i> Download </a></p>
Agro_pJW58_30004_full.rec, Reconstruction (Pixel Size 1.6 nm), 356.4 MB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-05-19/3dimage_39737/Agro_pJW58_30004_full.rec"
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Agrobacterium delta rem
No full textAgrobacterium tumefaciens non-motile delta rem mutant<strong>Tilt Series Date:</strong> 2014-04-04</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 19500x. </p>
<strong>Microscope:</strong> Janelia Titan</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Clay Fuqua</p>
<strong>Purification / Growth Conditions / Treatment:</strong> cells were grown and induced at 19C in liquid</p>
<strong>Sample Preparation:</strong> manual blot</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-14</p>delta_rem0001.mrc, Tilt Series (Pixel Size 0.56 nm), 3.3 GB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-14/rawdata/delta_rem0001.mrc"
> <i class="download icon"></i> Download </a></p>
delta_rem0001.rec, Reconstruction (Pixel Size 2.24 nm), 1.0 GB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-04-04-14/3dimage_40979/delta_rem0001.rec"
> <i class="download icon"></i> Download </a></p>
Agro pJW58
No full textwild-type Agro with plasmid-borne VirC1-GFP translational fusion under control of the virB promoter. Kanamycin-resistant (150 to 300 ug/mL)<strong>Tilt Series Date:</strong> 2014-03-05</p>
<strong>Data Taken By:</strong> Elitza Tocheva</p>
<strong>Species / Specimen:</strong> Agrobacterium tumefaciens</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>
<strong>Collaborators and Roles:</strong> Clay Fuqua</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AB medium</p>
<strong>Sample Preparation:</strong> Vitrobot</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-05-3</p>Agro_pJW580003.mrc, Tilt Series (Pixel Size 0.4 nm), 3.4 GB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-05-3/rawdata/Agro_pJW580003.mrc"
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Agro_pJW580003_part121_2.rec, Reconstruction (Pixel Size 1.6 nm), 356.4 MB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2014-03-05-3/3dimage_39679/Agro_pJW580003_part121_2.rec"
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