1,576 research outputs found
Beauvericin Immunotoxicity Prevention by Gentiana lutea L. Flower In Vitro
No full textBeauvericin (BEA) is an emerging mycotoxin produced by some species of Fusarium genera that widely contaminates food and feed. Gentiana lutea is a protected medicinal plant known for its antioxidant and anti-inflammatory properties, which are attributed to its rich content of bioactive compounds. In order to evaluate the beneficial effects of G. lutea flower against BEA cytotoxicity, the aim of this study is to evaluate changes in protein expression after Jurkat cell exposure through a proteomics approach. To carry out the experiment, cells were exposed to intestinally digested G. lutea flower alone or in combination with the BEA standard (100 nM) over 7 days. Differentially expressed proteins were statistically evaluated (p G. lutea alone, and 139 proteins when exposing the cells to the combined exposure. Bioinformatic analysis revealed processes implicated in mitochondria, ATP-related activity, and RNA binding. After careful analysis of differentially expressed proteins, it was evident that G. lutea attenuated, in most cases, the negative effects of BEA. Furthermore, it decreased the presence of major oncoproteins involved in the modulation of immune function
Lettera di Alessandra
No full textUn ritratto critico dell'opera di Alessandra Carnaroli, autrice fra le più apprezzate delle ultime generazioni della poesia di ricerca. La sezione a lei dedicata, nel numero della rivista, contiene inoltre saggi di Cecilia Bello Minciacchi, Andrea Cortellessa, e Ivan Schiavone; e vari inediti dell'autrice. Il saggio è pubblicato con lo pseudonimo di Tommaso Ottonieri.A critical portrait of the work of Alessandra Carnaroli, author of the most appreciated in the latest generations of italian research poetry. Published under the pseudonym Tommaso Ottonieri
Allium sativum L. var. Voghiera Reduces Aflatoxin B1 Bioaccessibility and Cytotoxicity In Vitro
No full textThe present work focuses on the evaluation of AFB1's bioaccessibility and cytotoxicity in vitro using bread (naturally contaminated) enriched or not enriched with fresh Voghiera garlic (2%). Two different experiments were carried out: experiment 1 (E1), with low-AFB1-concentration breads (1.6-1.7 mg/kg); and experiment 2 (E2), with high-AFB1-concentration breads (96.4-102.7 mg/kg). Eight breads were prepared, four for E1 (experiment 1) and another four for E2 (experiment 2), with each experiment having a control group (C), a garlic-enriched group (2%) (G), an AFB1 group (A), and an AFB1 + garlic group (A + G). Simulated digestion was performed on each type of bread, and gastric and intestinal digests were obtained. AFB1 content in flours, baked bread, and gastric and intestinal digests was measured by High-Performance Liquid Chromatography coupled to Fluorescence Detection. The results demonstrate dose-dependent AFB1 bioaccessibility and that the presence of garlic contributed to its reduction in both doses (7-8%). Moreover, garlic's presence in AFB1-contaminated bread increased cell viability (9-18%) in differentiated Caco-2 cells and mitigated the arrest of S and G2/M phases provoked by AFB1 on Jurkat T cells and reduced apoptosis/necrosis, cellular reactive oxygen species (ROS), and mitochondrial ROS by 16%, 71%, and 24% respectively. The inclusion of garlic as a functional ingredient helped relieve the presence and effects of AFB1
Selected letters of Alessandra Strozzi
No full textThe letters of Alessandra Strozzi provide a vivid and spirited portrayal of life in fifteenth-century Florence. Among the richest autobiographical materials to survive from the Italian Renaissance, the letters reveal a woman who fought stubbornly to preserve her family's property and position in adverse circumstances, and who was an acute observer of Medicean society. Her letters speak of political and social status, of the concept of honor, and of the harshness of life, including the plague and the loss of children. They are also a guide to Alessandra's inner life over a period of twenty-three years, revealing the pain and sorrow, and, more rarely, the joy and triumph, with which she responded to the events unfolding around her.This edition includes translations, in full or in part, of 35 of the 73 extant letters. The selections carry forward the story of Alessandra's life and illustrate the range of attitudes, concerns, and activities which were characteristic of their author
Proteomic Analysis of the Murine Liver Response to Oral Exposure to Aflatoxin B1 and Ochratoxin A: The Protective Role to Bioactive Compounds
No full textAflatoxin B1 (AFB1) and Ochratoxin A (OTA) are considered the most important mycotoxins in terms of food safety. The aim of this study was to evaluate the hepatotoxicity of AFB1 and OTA exposure in Wistar rats and to assess the beneficial effect of fermented whey (FW) and pumpkin (P) as functional ingredients through a proteomic approach. For the experimental procedures, rats were fed AFB1 and OTA individually or in combination, with the addition of FW or a FW-P mixture during 28 days. For proteomics analysis, peptides were separated using a LC-MS/MS-QTOF system and differentially expressed proteins (DEPs) were statistically filtered (p < 0.05) distinguishing males from females. Gene ontology visualization allowed the identification of proteins involved in important biological processes such as the response to xenobiotic stimuli and liver development. Likewise, KEGG pathway analysis reported the metabolic routes as the most affected, followed by carbon metabolism and biosynthesis of amino acids. Overall, the results highlighted a strong downregulation of DEPs in the presence of AFB1 and OTA individually but not with the mixture of both, suggesting a synergistic effect. However, FW and P have helped in the mitigation of processes triggered by mycotoxins
Challenging the author: Gavin Douglas's Eneados
No full textGavin Douglas’s Eneados, a translation into the “Scottis” tongue of Virgil’s Aeneid, completed in 1513 and first published in London in 1553, presents, as well as the translation of the additional thirteenth book by Maphaeus Vegius, original prologues and marginal notes to the text, rubrics and articulate conclusive material. The present paper analyses this complex paratext as evidence of Douglas’s almost philological attention to the original and his preoccupation with a faithful reproduction; it is also suggested that the models for his organization of the commentary might be both medieval (i.e., manuscripts such as Petrarch’s Virgilius Ambrosianus) and early modern, as in the case of editions of classical works: the most apt example being Jodocus Badius Ascensius’ edition of the Aeneid, printed in 1501. The Eneados thus stands on the threshold between manuscript and print, and might have indicated new possibilities of use of the printing medium in Scotland, and of the value of the translation of a classical text, had history not intervened with the Scottish defeat at Flodden Fields in 1513, which put a temporary stop both to the circulation of the Eneados and to the development of Scottish printing
Mitigating AFB1 and OTA hepatic residues using pumpkin and milk fermented whey as functional ingredients
No full textThe consumption of antioxidant-rich commodities acts as a preventive measure to regulate mycotoxin levels, which pose a significant threat to human and animal health worldwide. This study aimed to evaluate the potential reduction of mycotoxin liver bioaccumulation through an in vivo experimental study on 120 rats fed with
pumpkin, milk fermented whey, or combinations. The levels of Aflatoxin B1 (AFB1) and Ochratoxin A (OTA) in liver were monitored through Ultra High-Resolution Liquid Chromatography-Orbitrap Mass Spectrometry (UHPLC-Q-Orbitrap HRMS). The methodology was appropriately optimized according to current regulations.
Limit of quantification (LOQ) for AFB1 and OTA were 0.78 and 6.25 ng/g respectively, whereas recovery rates were up to 70% for both mycotoxins. Mycotoxin residues in the liver of rats fed with functional ingredients showed up to a 100 % reduction for AFB1 and 80% for OTA. The combination of pumpkin and fermented whey in
feed formulations has proven to be an effective approach easily implemented by the food industry to safeguard consumer health
Nicetas Nicaenus, De azymis
No full textThe RAP online repertorium offers the first comprehensive catalogue of polemical literature related to the schism between the Roman Catholic and Orthodox Churches from the 9th to the 16th century and can be described as an ideal continuation of the *Clavis Patrum*.
Each entry identifies the work (often unpublished or newly discovered in manuscript catalogs), lists its various titles (since medieval texts often lack stable titles), provides incipit and explicit (with possible variations), and examines the manuscript tradition and foliation (by reviewing catalogs or manuscripts, verifying dates, folios, etc.). It also includes relevant bibliography (critical editions and studies), identifies the author (using prosopographical studies, dictionaries, repertories, sigillography, etc.), and provides essential biographical details. Each work is classified by literary genre (e.g., treatise, dialogue), the corresponding Byzantine term, and the main polemical themes (e.g., Filioque, Azymes, Purgatory), and is assigned a unique RAP identification number.
The Repertorium Auctorum Polemicorum is identified by the International Standard Serial Number (ISSN) 3035-2096 [continuously updated publication
Polemica scripta anonyma, Dialogus inter Graecum et Cardinales quosdam de processione Spiritus Sancti
No full textThe RAP online repertorium offers the first comprehensive catalogue of polemical literature related to the schism between the Roman Catholic and Orthodox Churches from the 9th to the 16th century and can be described as an ideal continuation of the *Clavis Patrum*.
Each entry identifies the work (often unpublished or newly discovered in manuscript catalogs), lists its various titles (since medieval texts often lack stable titles), provides incipit and explicit (with possible variations), and examines the manuscript tradition and foliation (by reviewing catalogs or manuscripts, verifying dates, folios, etc.). It also includes relevant bibliography (critical editions and studies), identifies the author (using prosopographical studies, dictionaries, repertories, sigillography, etc.), and provides essential biographical details. Each work is classified by literary genre (e.g., treatise, dialogue), the corresponding Byzantine term, and the main polemical themes (e.g., Filioque, Azymes, Purgatory), and is assigned a unique RAP identification number.
The Repertorium Auctorum Polemicorum is identified by the International Standard Serial Number (ISSN) 3035-2096 [continuously updated publication
Theophylactus Bulgariae archiepiscopus, Allocutio ad quemdam ex suis familiaribus de iis quorum Latini incusantur
No full textThe RAP online repertorium offers the first comprehensive catalogue of polemical literature related to the schism between the Roman Catholic and Orthodox Churches from the 9th to the 16th century and can be described as an ideal continuation of the *Clavis Patrum*.
Each entry identifies the work (often unpublished or newly discovered in manuscript catalogs), lists its various titles (since medieval texts often lack stable titles), provides incipit and explicit (with possible variations), and examines the manuscript tradition and foliation (by reviewing catalogs or manuscripts, verifying dates, folios, etc.). It also includes relevant bibliography (critical editions and studies), identifies the author (using prosopographical studies, dictionaries, repertories, sigillography, etc.), and provides essential biographical details. Each work is classified by literary genre (e.g., treatise, dialogue), the corresponding Byzantine term, and the main polemical themes (e.g., Filioque, Azymes, Purgatory), and is assigned a unique RAP identification number.
The Repertorium Auctorum Polemicorum is identified by the International Standard Serial Number (ISSN) 3035-2096 [continuously updated publication
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