170,123 research outputs found
CIL:42157
No full textTime lapse movie of P-cadherin +/- epithelium (mammary organoid) branching normally in 3D collagen I matrix. The movie shows minimal cell dissemination and retention of complete myoepithelial coverage. The cells are expressing td tomato as a membrane marker and eEGFP-actin using a keratin-14 promoter. Images were collected every 20 minutes. Image size is 350 x 350 microns. A movie of a normal mouse mammary organoid in collagen I can be found at CIL 42155. This movie is part of a group of movies that include CIL 42151-42168
CIL:42156
No full textP-cadherin null epithelium shows enhanced and sustained dissemination of myoepithelial cells. Images taken every 20 minutes. This movie is part of a group of movies that include CIL 42151-42168
CIL:42159
No full textTime-lapse movie showing that disseminated P-cadherin null cells persist in the extracellular matrix and can proliferate The cells are expressing td tomato as a membrane marker and eEGFP-actin using a keratin-14 promoter. Images were collected every 20 minutes. The image size is 350 x 350 microns. This movie is part of a group of movies that include CIL 42151-42168
CIL:42158
No full textTime-lapse movie showing extensive dissemination of myoepithelial cells from P-cadherin null epithelium, with gradual loss of myoepithelial coverage. The cells are expressing td tomato as a membrane marker and eEGFP-actin using a keratin-14 promoter. Images were collected every 20 minutes. The image size is 350 x 350 microns. This movie is part of a group of movies that include CIL 42151-42168
CIL:40267
No full textTime lapse movie of a porcine kidney LLCPK1 cell stably expressing photoactivable GFP-tubulin. Fluorescence was activated at prophase in a vertical strip perpendicular to the spindle orientation. Microtubules in the activated strip show a variety of motions both toward and away from spindle poles. This contrasts with the exclusively poleward motion of spindle microtubules seen at metaphase (and illustrated in the grouped movie CIL:40266). The time series of images used to prpeare this movie is included in the group of images.
See Fig 1A in: NP Ferenz and P Wadsworth 2007. Prophase microtubule arrays undergo flux-like behavior in mammalian cells. Mol Biol Cell 18:3993-400
CIL:36613
No full textThe P-face of the ciliary membrane at the base of the cilium has 9 triple rows of IMPs in register with the 9 doublets of the internal axoneme. It is thought that these IMPs may be sites of calcium influx into the base of the cilium for regulating ciliary activity. Also, trichocyst docking sites. TEM taken on 1/6/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm. There is a high resolution version of this image in the library (CIL:39317) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/)
CIL:36605
No full textFreeze fracture image of the surface ridges at the anterior end of the cell where kineties to the right and left sides come together at an angle at the anterior suture tip. In this area are numerous plates of IMPs in the P-face of the plasma membrane. Rings and rosette IMPs at the trichocyst exocytic sites are also seen. TEM taken on 10/2/80 by R. Allen with HU 11A operating at 75kV. Neg. 10,000X. Bar = 1µm. There is a high resolution version of this image in the library (CIL:39313) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/)
CIL:36614
No full textIn addition to plates the plasma membrane contains IMPs on its P-fracture face in ring or parenthesis shape at sites of trichocyst secretion. A ring actually consists of two circles of IMPs and signifies that a trichocyst is docked underneath while a parenthesis means the site has no docked trichocyst. The fracture also exposed some of the E-face of the outer alveolar membrane which is relatively free of IMPs. TEM taken on 6/4/90 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm. There is a high resolution version of this image in the library (CIL:39318) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/)
CIL:21996
No full textThe plasma membrane of Paramecium multimicronucleatum seen in a freeze fracture replica. The two rows of IMPs can be seen on the P-face of the plasma membrane that connects the plasma membrane to the underlying margins of alveolar sacs. A gap 20nm wide composed of only the plasma membrane connects these two ribbons. The spent vacuole membrane will fuse with the membrane over this gap. Further interpretation of this image can be seen at Figure 4 of Chapter 7 at the link below. TEM taken on 6/3/75 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 12,750X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:12346) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/)
CIL:36607
No full textIn quick-freeze deep-etch preparations the plasma membrane can be seen to contain plaques or plates of intramembrane particles (IMPs) that are regularly aligned in rows with a center-to-center spacing between rows of 25nm. These plaques remain in the P-fracture face of the plasma membrane and are found mostly on the anterior ventral surface of the cell near the anterior suture. Their function is not known. For discussion see Allen, J. Ultrastruc. Res. 63:64-78, 1978 and Hufnagel, Micros. Res. Tech. 22:225-264, 1992. TEM taken on 5/26/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm. There is a high resolution version of this image in the library (CIL:39314) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/)
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