1,279 research outputs found

    Effects of medium components on l-ornithine production by Brevibacterium ketoglutamicum

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    Effects of yeast extract and ammonium sulfate were investigated on the production of L-ornithine by an arginine auxotroph, Brevibacterium ketoglutamicum in flask and batch cultures. Yeast extract as an arginine source and ammonium sulfate as an inorganic nitrogen source had significant effects on L-ornithine production and cell growth. L-ornithine production was repressed by the excessive addition of arginine Reversion of auxotrophic cells to the wild type was observed when the initial yeast extract concentration was too low. There existed optimum concentrations of yeast extract and ammonium sulfate for L-ornithine production. The effects of yeast extract and ammonium sulfate concentrations on the Leudeking-Piret model parameters were examined to analyze the relationship between cell growth and L-ornithine production

    Kraichnan-Leith-Batchelor similarity theory and two-dimensional inverse cascades

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    We study the scaling properties and Kraichnan-Leith-Batchelor (KLB) theory of forced inverse cascades in generalized two-dimensional (2D) fluids (α\alpha-turbulence models) simulated at resolution 819228192^2. We consider α=1\alpha=1 (surface quasigeostrophic flow), α=2\alpha=2 (2D vorticity dynamics) and α=3\alpha=3. The forcing scale is well-resolved, a direct cascade is present and there is no large-scale dissipation. Coherent vortices spanning a range of sizes, most larger than the forcing scale, are present for both α=1\alpha=1 and α=2\alpha=2. The active scalar field for α=3\alpha=3 contains comparatively few and small vortices. The energy spectral slopes in the inverse cascade are steeper than the KLB prediction (7α)/3-(7-\alpha)/3 in all three systems. Since we stop the simulations well before the cascades have reached the domain scale, vortex formation and spectral steepening are not due to condensation effects; nor are they caused by large-scale dissipation, which is absent. One- and two-point pdfs, hyperflatness factors and structure functions indicate that the inverse cascades are intermittent and non-Gaussian over much of the inertial range for α=1\alpha=1 and α=2\alpha=2, while the α=3\alpha=3 inverse cascade is much closer to Gaussian and non-intermittent. For α=3\alpha=3 the steep spectrum is close to that associated with enstrophy equipartition. Continuous wavelet analysis shows approximate KLB scaling E(k)k2\mathcal{E}(k) \propto k^{-2} (α=1\alpha=1) and E(k)k5/3\mathcal{E}(k) \propto k^{-5/3} (α=2\alpha=2) in the interstitial regions between the coherent vortices. Our results demonstrate that coherent vortex formation (α=1\alpha=1 and α=2\alpha=2) and non-realizability (α=3\alpha=3) cause 2D inverse cascades to deviate from the KLB predictions, but that the flow between the vortices exhibits KLB scaling and non-intermittent statistics for α=1\alpha=1 and α=2\alpha=2. The results will appear in \cite{BurgessEA2015}, which has been accepted to the \emph{Journal of Fluid Mechanics}
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