1,880 research outputs found
Impaired neural development in a zebrafish model for lowe syndrome
No full textLowe syndrome, which is characterized by defects in the central nervous system, eyes and kidneys, is caused by mutation of the phosphoinositide 5-phosphatase OCRL1. The mechanisms by which loss of OCRL1 leads to the phenotypic manifestations of Lowe syndrome are currently unclear, in part, owing to the lack of an animal model that recapitulates the disease phenotype. Here, we describe a zebrafish model for Lowe syndrome using stable and transient suppression of OCRL1 expression. Deficiency of OCRL1, which is enriched in the brain, leads to neurological defects similar to those reported in Lowe syndrome patients, namely increased susceptibility to heat-induced seizures and cystic brain lesions. In OCRL1-deficient embryos, Akt signalling is reduced and there is both increased apoptosis and reduced proliferation, most strikingly in the neural tissue. Rescue experiments indicate that catalytic activity and binding to the vesicle coat protein clathrin are essential for OCRL1 function in these processes. Our results indicate a novel role for OCRL1 in neural development, and support a model whereby dysregulation of phosphoinositide metabolism and clathrin-mediated membrane traffic leads to the neurological symptoms of Lowe syndrome. © The Author 2011. Published by Oxford University Press. All rights reserved
sj-pdf-1-vet-10.1177_03009858221102600 – Supplemental material for Carnobacterium maltaromaticum associated with meningoencephalitis and otitis in stranded common thresher sharks (Alopias vulpinus)
No full textSupplemental material, sj-pdf-1-vet-10.1177_03009858221102600 for Carnobacterium maltaromaticum associated with meningoencephalitis and otitis in stranded common thresher sharks (Alopias vulpinus) by Laura Martinez Steele, Mark S. Okihiro, Renaud Berlemont, Jesse G. Dillon, Kelly A. Young, Shohreh Hesami, Sean Van Sommeran and Christopher G. Lowe in Veterinary Pathology</p
Correction to: The possibilities and practicalities of professional learning in support of Indigenous student experiences in schooling: A systematic review
No full textCorrection to: The Australian Educational Researcher https://doi.org/10.1007/s13384-019-00313-7
In the original publication of the article, the author group was incorrectly published without the co-authors. The correct author group is “Greg Vass, Kevin Lowe, Cathie Burgess, Neil Harrison, Nikki Moodie”.No Full Tex
Defining absolute environmental limits for the built environment
No full textThe question addressed is whether it is possible to define working limits on environmental impacts from the built environment in terms of global carrying capacity. The main focus is on energy-related impacts, since these are global and relatively well-understood. Four possible approaches to defining limits are explored: static equilibrium, asymptotic, integral of excess and planned future. The conclusions that emerge from this exploration are that global environmental constraints are very tight, but also that they are dynamically and strongly influenced by the trajectory of social and technological development over the coming century. Their use as the basis for practical, quantitative metrics of sustainability, therefore, involves a large measure of subjectivity. A fifth approach - the developmental approach - is identified, which instead of focusing on long-term external constraints to human activity, focuses instead on the internal, short- to medium-term dynamics of the built environment itself. It appears likely that the developmental approach, guided by qualitative conclusions from the analysis of global carrying capacity, is likely to be most fruitful
Correction to: ‘Aboriginal Voices’: An overview of the methodology applied in the systematic review of recent research across ten key areas of Australian Indigenous education (The Australian Educational Researcher, (2019), 46, 2, (213-229), 10.1007/s13384-019-00307-5)
No full textIn the original publication of the article, the author name “Cathie Burgess” was inadvertently missed in the author group. The correct author group is “Kevin Lowe · Christine Tennent · John Guenther · Neil Harrison · Cathie Burgess · Nikki Moodie · Greg Vass”. Cathie Burgess coordinates Aboriginal Studies, Aboriginal Community Engagement and the Master of Education: Leadership in Aboriginal Education programs at the University of Sydney. Cathie’s research involves community-led initiatives positioning Aboriginal cultural educators as experts through projects such as Learning from Country in the City, Aboriginal Voices: Insights into Aboriginal Education and Redfern Cultural Program. The original article has been corrected
Gender and the culture of the English alehouse in late Stuart England
No full textThe world of the alehouse and tavern in early modern England has generally been
regarded as primarily male, a view that was deeply embedded in the period itself.
This essay explores the place of women within the public house, in serving, buying
and consuming alcohol, and the unwritten conventions that underpinned social
practice. It argues that while some female customers matched their contemporary
image, as disorderly, immoral and dishonest, it was also possible for respectable
women to visit a tavern or alehouse without risking their good name, provided they
adhered to the conventions. Middling-sort and elite women might drink and dine in
London taverns with their husbands, or in mixed parties; throughout England married
couples, and mixed groups of young folk, might drink, dance, and socialise; marketwomen
might assemble at the end of the day, and chapwomen often lodged overnight.
And, at least in London, respectable women might enter a public house alone, by
day, without meeting disapproval. Many establishments provided private as well as
public rooms, and these created social spaces for female customers, couples and
mixed parties, serving different needs than those met within the main public space
Barbaracurus somalicus Kovařík & Lowe & Šťáhlavský 2018, comb. n.
No full text<i>Barbaracurus somalicus</i> (Hirst, 1907) comb. n. <p>(Figures 3, 27, 34, 54–57, 74–75, 83–84, 113–146, 263–265, Table 1)</p> <p> <i>Babycurus somalicus</i> Hirst, 1907: 208; Lamoral & Reynders, 1975: 498; Kovařík, 1998: 104; Fet & Lowe, 2000: 79; Kovařík, 2000: 255–256, figs. 10, 21, tables 1–3; Kovařík, 2003: 134.</p> <p>TYPE LOCALITY AND TYPE DEPOSITORY. Somaliland, Berbera and Durbar; BMNH.</p> <p> MATERIAL EXAMINED. <b>Somaliland</b>, Berbera and Durbar, 400 ft., leg. G. W. Bury, 1♀ (holotype), BMNH; Borama, campus Amound University, 09°56'49"N 43° 13'23"E, 1394 m a.s.l., 4-5.II.2017 (locality No. <b>17 SA</b>), 1♀, 9.-13.IX.2017, (locality No. <b>17SR</b>, Fig. 145), 4♂ 3♀ 4juvs. (Figs. 27, 35, 113–144, 260–261, Nos. 1308, 1309, 1332), leg. F. Kovařík, T. Mazuch & P. Just, FKCP.</p> <p>DIAGNOSIS. Total length of adult males 32–36 mm, adult female 38–47 mm. Coloration yellowish brown to grey with darker markings, chelicerae yellow without or with traces of reticulation. Pedipalp chela manus much wider in male than female, chela length/width ratio 3.45 in male and 4.15 in female; proximal margins of pedipalp fingers of female almost straight (Fig. 57, 135), of male strongly undulate so as to leave a gap with fingers closed (Figs. 55, 129); dentate margin of movable finger armed with 6 rows of granules, and a short apical row of 4 denticles (Fig. 3); most proximal granule row with one external accessory granule. Pectines with 17–20 teeth in both sexes. Hemispermatophore basal lobe a weak carina (Figs. 27, 35). No sexual dimorphism in length and width of metasomal segments (Figs. 74–75); metasoma I with 10 carinae, II–IV with 8 carinae. Telson setose, bearing numerous long macrosetae and short, pointed subaculear tubercle; vesicle smooth, elongate, pyriform, telson length/depth ratio 2.75–2.89 in both sexes; aculeus slender, curved, shorter than vesicle.</p> <p> NOTE. Until now, only the holotype and paratype females were known. The recent collection of both sexes of <i>B. somalicus</i> by one of us (F.K.) enables us to show photographs of live specimens, especially of the male, for the first time, and to characterize their sexual dimorphism.</p> <p> COMMENTS ON LOCALITY AND LIFE STRATEGY. The first author (F.K.) visited the locality 17SA on 4–5 February 2017 (winter dry season). At this locality, the author recorded a daytime temperature of 24.7 ºC (4 February, 16:08 h), and nighttime temperatures of 21.4 ºC shortly after sunset, dropping to 19.3 ºC (minimum temperature on 5 February at 19:20 h). The recorded humidity was 41% on 5 February at 19:20 h. The first author (F.K.) again visited the same locality on 9–13 September 2017 (summer minor dry season, 17SR) and recorded maximum daytime temperatures of 29.1 ºC (10th September 2017) and 31.8 ºC (12 September 2017), and a minimum nighttime temperature of 19.6 ºC. The recorded humidity was between 31% (minimum at night) and 79% (maximum at day). All specimens were collected at night by ultraviolet (UV) detection near rocks. At this locality, in addition to <i>B. somalicus</i>, the first author also recorded <i>Neobuthus</i> sp., <i>Parabuthus abyssinicus</i> Pocock, 1901 (Buthidae) and <i>Pandinurus kmoniceki</i> Kovařík et al., 2017 (type locality) (Scorpionidae). Fifty metres from this rocky site is a riverbed of an occasional river (figs. 45–48 in Kovařík et al., 2017: 18) where in addition the author recorded <i>Gint amoudensis</i> Kovařík et al., 2018 (type locality) (Buthidae), and <i>Pandinops pugilator</i> (Pocock, 1900) (Scorpionidae).</p>Published as part of <i>Kovařík, František, Lowe, Graeme & Šťáhlavský, František, 2018, Review of the genus Babycurus Karsch, 1886 (Arachnida, Scorpiones, Buthidae), with descriptions of Barbaracurus gen. n. and two new species from Oman and Yemen, pp. 1-41 in Euscorpius 267</i> on pages 25-27, DOI: <a href="http://zenodo.org/record/6544157">10.5281/zenodo.6544157</a>
Inflation Targeting: The British Experience
No full textThis lecture describes the United Kingdom's experience with inflation targeting. It provides a historical perspective to the introduction of inflation targeting, discusses the concept of inflation targets, and compares an inflation targeting regime with money supply and exchange rate targeting regimes. It is noteworthy that inflation targeting is based on the assumption that low inflation is the proper objective of monetary policy. A significant portion of the lecture covers the issue of the measurement of inflation. It discusses whether asset prices should be taken into account in the inflation measure and looks in particular at the experience of Japan in the late 1980s. It also considers sources of imperfection in traditional measures. It concludes that monetary policy will have to be conducted by reference to estimated price indexes that fall short of the conceptual ideal but does not regard this as seriously undermining an inflation targeting regime. The lecture goes on to discuss the issues of (1) having a target band for inflation or not, (2) the difficulty in forecasting inflation, and (3) the time horizon over which monetary policy should aim. The lecture highlights the important role that openness and transparency play in achieving credibility in monetary policy. It highlights the five devices that are now in use in the United Kingdom, and notes some of the benefits emerging from the open and transparent nature of the United Kingdom approach. It concludes by warning that inflation targeting does not promise to make monetary policy easy but does have the positive virtue of directing attention to many technical issues that need to be resolved in conducting monetary policy.Inflation, Targeting, British
OCRL1 engages with the F-BAR protein pacsin 2 to promote biogenesis of membrane-trafficking intermediates.
No full textMutation of the inositol 5-phosphatase OCRL1 causes Lowe syndrome and Dent-2 disease. Loss of OCRL1 function perturbs several cellular processes, including membrane traffic, but the underlying mechanisms remain poorly defined. Here we show that OCRL1 is part of the membrane-trafficking machinery operating at the trans-Golgi network (TGN)/endosome interface. OCRL1 interacts via IPIP27A with the F-BAR protein pacsin 2. OCRL1 and IPIP27A localize to mannose 6-phosphate receptor (MPR)-containing trafficking intermediates, and loss of either protein leads to defective MPR carrier biogenesis at the TGN and endosomes. OCRL1 5-phosphatase activity, which is membrane curvature sensitive, is stimulated by IPIP27A-mediated engagement of OCRL1 with pacsin 2 and promotes scission of MPR-containing carriers. Our data indicate a role for OCRL1, via IPIP27A, in regulating the formation of pacsin 2-dependent trafficking intermediates and reveal a mechanism for coupling PtdIns(4,5)P2 hydrolysis with carrier biogenesis on endomembranes
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