374 research outputs found
Archaea and the tree of life
No full textSéminaire organisé par Patrick Forterre du 11 au 16 mai 2009 Participants Stephen D. Bell, Jonathan Berthon, Céline Brochier-Armanet (organisateur), Vincent Daubin, Elie Desmond, Arnold J.-M. Driessen, Laure Eme, Patrick Forterre (organisateur), Roger Garrett, J. Peter Gogarten, Simonetta Gribaldo (organisateur), Henri Grosjean, Karl-Peter Hopfner, Michel Morange, Gary J. Olsen, Anthony M. Poole, Christa Schleper, John van der Oost, Christiaan van Ooij, Finn Werner, Malcolm F. White Compte r..
Indications for a moonlighting function of translation factor aIF5A in the crenarchaeum Sulfolobus solfataricus
No full textTranslation factor a/eIF5A is highly conserved in Eukarya and Archaea. The eukaryal eIF5A protein is required for transit of ribosomes across consecutive proline codons, whereas the function of the archaeal orthologue remains unknown. Here, we provide a first hint for an involvement of Sulfolobus solfataricus (Sso) aIF5A in translation. CRISPR-mediated knock down of the aif5A gene resulted in strong growth retardation, underlining a pivotal function. Moreover, in vitro studies revealed that Sso aIF5A is endowed with endoribonucleolytic activity. Thus, aIF5A appears to be a moonlighting protein that might be involved in protein synthesis as well as in RNA metabolism
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