3 research outputs found

    Prevalence of Staphylococcus aureus, Klebsiella spp., Escherichia coli and Pseudomonas spp. in some common Bangladeshi milk and dairy products

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    The study was done to isolate Klebsiella spp., Staphylococcus aureus, Escherichia coli & Pseudomonas spp. from raw milk, processed milk (powdered milk & ultra-high-temperature milk) and dairy products (mango milk, and ice cream). During 3 months’ time span of study, a total of ten samples were gathered where eight of the samples powdered milk (2), ultra-high-temperature milk (2), ice cream (2) & mango milk (2) were gathered from different places of Bangladesh & other 2 samples (raw milk) were collected from a cattle farm & individual households in & around Noakhali city. The samples were gathered under aseptic safety measures and were followed by plating on non-selective media (Nutrient broth) & selective media (Mannitol Salt Agar & Macconkey agar). The presumptive Klebsiella spp., Staphylococcus aureus, Escherichia coli & Pseudomonas spp. isolates were identified by biochemical tests. Analysis of the result discovered that out of a total of 10 samples; raw milk (2), processed milk i.e. powdered milk (2), ultra-high-temperature milk (2) and dairy products i.e. mango milk (2) and ice cream (2) resulted in the isolation of 8 isolates (2.5%) of S. aureus, (12.5%) of Klebsiella spp., (2.5%) of Pseudomonas spp. & (2.5%) of Escherichia coli. Results recommended a probability of potential public health threat of Staphylococcus aureus, Klebsiella spp., Escherichia coli & Pseudomonas spp. resulting from contamination of milk and dairy products with pathogenic microorganisms is mainly due to unhygienic practices and conditions in the handling, production, and processing

    A confirmatory death case of human rabies in Bangladesh

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    In the present study clinical profile with specific attention to typical clinical presentation and deficiencies/lacunae in post-exposure prophylactic management as per WHO guidelines in adult rabies patients. An observational analysis was performed on a clinically suspected human rabies case, whose samples were gathered at a rabies diagnostic laboratory at Infectious Disease Hospital, Dhaka (IDH) over the course of three days (20/09/2021 to 23/09/2021). The patient’s clinical and demographic information was acquired. The clinical specimens involved cerebrospinal fluid (CSF), saliva, nuchal skin biopsy and serum collected antemortem, and brain tissue obtained post-mortem. A variety of laboratory tests were used to make the diagnosis. The patient died in hospital 3 days after hospitalization (20 days after the occurrence of animal bite). After referring the patient to the IDH, a lumbar puncture was administered, and PCR tests for rabies were forwarded to the cerebrospinal fluid (CSF) and serum. The serum & CSF have indicated antibodies with rabies. The fluorescence microscopy screening program for rabies was positive in the brainstem, cerebral cortex, cerebellum, and cerebrum. The patient’s samples of saliva (0.5-1 mL) and skin were also sent to PCR for rabies. Diagnostic testing of the patient later confirmed human rabies. Human rabies is a crucial health problem. So, there are required to educate the community and health workers about the value of immediate and adequate post-exposure treatment, to launch an effective control program for rabid dogs, and to make availability of rabies vaccine as well as rabies immunoglobulin (RIG)

    Performance Analysis of Perovskite Solar Cells Using DFT-Extracted Parameters of Metal-Doped TiO2 Electron Transport Layer

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    The performance of perovskite solar cells (PSCs) depends heavily on the electronic and optical properties of the electron transport layer (ETL). Density functional theory (DFT) uses a quantum-mechanical approach to accurately predict the properties of different layers in PSCs, including the ETL. Titanium dioxide (TiO2) is a widely used material for the ETL in PSCs. In this work, we use first-principles calculations based on DFT to obtain the electronic and optical properties of pristine rutile TiO2 and TiO2 doped with tin (Sn) and zinc (Zn). DFT-extracted carrier mobility, band gap, and the absorption spectrum of TiO2 are used in the SCAPS-1D device simulator to evaluate the performance of the solar cell device, with respect to dopant concentration and thickness of TiO2. PSCs with 3.125 mol % Sn-doped TiO2 achieve a maximum power conversion efficiency (PCE) of 17.14 versus 13.70% with undoped TiO2. We have also compared the performance of PSCs with Sn-doped and Zn-doped TiO2. For the same dopant concentration, Sn-doped TiO2 offers 0.63% higher PCE than the Zn-doped counterpart. The results are in good agreement with reported experimental findings and provide a reliable means of evaluating PSC performance by combining first-principles (DFT) calculations with conventional device simulations
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