90 research outputs found
OXIDATIVE STRESS REDUCING AND LIVER PROTECTIVE EFFICACY OF ETHYL ACETATE FRACTION ISOLATED FROM PAJANELIA LONGIFOLIA (WILLD) K. SCHUMAN
No full textObjectives: We investigated the liver protective as well as oxidative stress reducing activity of isolated fractions (Pajanelia fraction-1, 2, and 3 [PF1, PF2, and [PF3]) from ethyl acetate extract of Indian medicinal plant Pajanelia longifolia (Willd.) K. Schuman.Methods: The liver protective activity of fractions was evaluated against carbon tetrachloride (CCl4) (0.5 ml/kg body weight intraperitoneal [b.w. i.p.]) induced hepatic damage in Swiss albino mice. Isolated fractions (at dose level of 150 mg/kg b.w. per orally [p.o.]) were administered to mice before 30 minutes of CCl4 administration. Silymarin at a dose of 50 mg/kg b.w. p.o. was taken as a standard drug. The liver protective activity of fractions was measured on serum biochemical parameters such as serum glutamic pyruvic transaminase, serum glutamic oxaloacetic transaminase, serum alkaline phosphatase, and bilirubin (total). Further antioxidant activity of fractions was also measured on antioxidant enzymatic and non-enzymatic levels such as reduced glutathione, lipid peroxidation, superoxide dismutase, catalase, and glutathione peroxidase.Results: The in vivo study revealed that the PF3 most effectively protected the liver from damage by CCl4. The findings were also confirmed by histopathological observations. High-performance liquid chromatography result was clearly indicated that the fraction was pure.Conclution: Ethyl acetate fraction (Pajanelia fraction 3) from P. longifolia may be considered as a liver protective agent.</jats:p
ANTIHEPATOTOXIC POTENTIALITY OF 2-3-6 TRIMETHYLOCT-6-ENAL AGAINST PARACETAMOL INDUCED LIVER DYSFUNCTION
Full text linkObjective: We investigated the liver protective activity of 2-3-6 trimethyloct-6-enal from the methanol extract of Pajanelia longifolia (Willd.) K. Schuman. The liver protective activity of 2,3,6 trimethyloct-6-enal was evaluated against paracetamol (2 mg/kg body weight per orally) induced liver toxicity in swiss albino mice.Methods: Considering the Spectral data (IR spectrum, 1HNMR spectrum and 13C NMR spectrum) the predictable structure of 2,3,6 trimethyloct-6-enal was elucidated. To study the liver protective activity of the compound, Swiss albino mice of either sex were divided into six groups and treated for 5 d. Group I and II served as normal and toxic control, Group III were treated with Silymarin as a standard drug (50 mg/kg), and Group IV to VI was treated with 2-3-6 trimethyloct-6-enal at the dose of 50 mg/kg, 150 mg/kg and 250 mg/kg b.w. p. o. respectively. The liver protective activity of the compound was measured on biochemical parameters such as aspertate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total bilirubin (TB), triglycerides (TGL), total cholesterol (TC) and protein. Further antioxidant activity of the compound was also measured on antioxidant enzymatic and non-enzymatic levels such as reduced glutathione (GSH), lipid peroxidation (LPO), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)).Results: The study revealed that the compound has protective activity at the dose of 50, 150 and 250 mg/kg b.w. p. o. against paracetamol induced toxicity. In some biochemical parameters such as aspartate amino transferase and bilirubin, the compound has showed better result at a dose of 150 mg/kg compared to standard drug silymarin (value of aspartate amino transferase (compound) =71.10±0.12, (toxic) = 173.43±1.21, (silymarin) =79.86±0.02and total bilirubin (compound) = 1.04±0.11), (toxic) = 2.69±0.02, (silymarin) ==1.11±0.01. The findings were also confirmed by histopathological observations.Conclusion: 2,3,6 trimethyloct-6-enal from Pajanelia longifolia may be considered as a potent liver protective agent
ANTIHEPATOTOXIC POTENTIALITY OF 2-3-6 TRIMETHYLOCT-6-ENAL AGAINST PARACETAMOL INDUCED LIVER DYSFUNCTION
No full textObjective: We investigated the liver protective activity of 2-3-6 trimethyloct-6-enal from the methanol extract of Pajanelia longifolia (Willd.) K. Schuman. The liver protective activity of 2,3,6 trimethyloct-6-enal was evaluated against paracetamol (2 mg/kg body weight per orally) induced liver toxicity in swiss albino mice.Methods: Considering the Spectral data (IR spectrum, 1HNMR spectrum and 13C NMR spectrum) the predictable structure of 2,3,6 trimethyloct-6-enal was elucidated. To study the liver protective activity of the compound, Swiss albino mice of either sex were divided into six groups and treated for 5 d. Group I and II served as normal and toxic control, Group III were treated with Silymarin as a standard drug (50 mg/kg), and Group IV to VI was treated with 2-3-6 trimethyloct-6-enal at the dose of 50 mg/kg, 150 mg/kg and 250 mg/kg b.w. p. o. respectively. The liver protective activity of the compound was measured on biochemical parameters such as aspertate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total bilirubin (TB), triglycerides (TGL), total cholesterol (TC) and protein. Further antioxidant activity of the compound was also measured on antioxidant enzymatic and non-enzymatic levels such as reduced glutathione (GSH), lipid peroxidation (LPO), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)).Results: The study revealed that the compound has protective activity at the dose of 50, 150 and 250 mg/kg b.w. p. o. against paracetamol induced toxicity. In some biochemical parameters such as aspartate amino transferase and bilirubin, the compound has showed better result at a dose of 150 mg/kg compared to standard drug silymarin (value of aspartate amino transferase (compound) =71.10±0.12, (toxic) = 173.43±1.21, (silymarin) =79.86±0.02and total bilirubin (compound) = 1.04±0.11), (toxic) = 2.69±0.02, (silymarin) ==1.11±0.01. The findings were also confirmed by histopathological observations.Conclusion: 2,3,6 trimethyloct-6-enal from Pajanelia longifolia may be considered as a potent liver protective agent.</jats:p
OXIDATIVE STRESS REDUCING AND LIVER PROTECTIVE EFFICACY OF ETHYL ACETATE FRACTION ISOLATED FROM PAJANELIA LONGIFOLIA (WILLD) K. SCHUMAN
Full text linkObjectives: We investigated the liver protective as well as oxidative stress reducing activity of isolated fractions (Pajanelia fraction-1, 2, and 3 [PF1, PF2, and [PF3]) from ethyl acetate extract of Indian medicinal plant Pajanelia longifolia (Willd.) K. Schuman.Methods: The liver protective activity of fractions was evaluated against carbon tetrachloride (CCl4) (0.5 ml/kg body weight intraperitoneal [b.w. i.p.]) induced hepatic damage in Swiss albino mice. Isolated fractions (at dose level of 150 mg/kg b.w. per orally [p.o.]) were administered to mice before 30 minutes of CCl4 administration. Silymarin at a dose of 50 mg/kg b.w. p.o. was taken as a standard drug. The liver protective activity of fractions was measured on serum biochemical parameters such as serum glutamic pyruvic transaminase, serum glutamic oxaloacetic transaminase, serum alkaline phosphatase, and bilirubin (total). Further antioxidant activity of fractions was also measured on antioxidant enzymatic and non-enzymatic levels such as reduced glutathione, lipid peroxidation, superoxide dismutase, catalase, and glutathione peroxidase.Results: The in vivo study revealed that the PF3 most effectively protected the liver from damage by CCl4. The findings were also confirmed by histopathological observations. High-performance liquid chromatography result was clearly indicated that the fraction was pure.Conclution: Ethyl acetate fraction (Pajanelia fraction 3) from P. longifolia may be considered as a liver protective agent
Assessment of pulp and paper mill effluent quality and its toxicity to fingerlings of Cyprinus carpio
No full textEffluents from a pulp and paper mill in India were analyzed for various physicochemical parameters and heavy metals. Multivariate statistical analysis was used to interpret the data obtained and predict the toxicity of each component to Cyprinus carpio L. fingerlings. The results showed that most of these parameters exceeded recommended levels set by various agencies, and the effluent was not suitable for discharge into agricultural fields or water bodies without further treatment. The results of static renewal toxicity tests revealed median lethal concentrations at 24, 48, 72, and 96 h to be 42, 25, 18.6 and 14.5%, respectively, and the fingerlings exhibited severe behavioral anomalies. The sublethal dose of 2.9% (v/v) caused alterations in the gill and liver at durations of 7, 14, 21, and 28 d. Anomalies such as mucous secretion, leucocyte infiltration, curling of the secondary lamellae tip, clubbing, and the fusion of the secondary lamellae, aneurism, etc. were prominent. The liver showed inflammatory infiltration, cytoplasmic inclusion bodies, vacuolar degeneration, fatty degeneration, pyknosis, karyolysis, distorted pancreatic area, and piecemeal necrosis, among other pathological symptoms. The study concluded that the effluent, even in greatly diluted form, was highly toxic and the severity of responses depended on the length of exposure
HEPATOPROTECTIVE ACTIVITY OF AQUEOUS EXTRACT OF OXALIS DEBILIS KUNTH AGAINST CCL4 - INDUCED LIVER DAMAGE
No full text Objective: To evaluate the hepatoprotective activity of aqueous extract of Oxalis debilis Kunth in carbon tetrachloride (CCl4)-induced hepatotoxicity in Swiss albino mice.Methods: Hepatotoxicity was induced by CCl4 30% in olive oil (1 ml/kg intraperitoneally). Mice were treated with aqueous extract of O. debilis at doses of 250 and 500 mg/kg body weight orally for 14 days. There were two groups, pre-treatment (once daily for 14 days before CCl4 intoxication) and post-treatment (2, 6, 24, and 48 hrs after CCl4 intoxication). The observed effects were compared with a known hepatoprotective agent, silymarin.Results: Pre-treatment and post-treatment groups of aqueous extract of O. debilis significantly reduced elevated serum levels of serum transaminases, alkaline phosphatase, and bilirubin and increased the level of total protein as compared to CCl4-treated group. The histopathological study also confirms the hepatoprotection. Preliminary qualitative phytochemical analysis of the plant revealed the presence of phenolic compounds, tannins, flavonoids, and saponins.Conclusion: The results of this study suggest that O. debilis can be used as safe, cheap, and alternative preventive and protective drugs against liver injury. The protective effect observed could be attributed to the presence of various phytochemicals which are responsible for the restoration of liver damage.</jats:p
HEPATOPROTECTIVE ACTIVITY OF AQUEOUS EXTRACT OF OXALIS DEBILIS KUNTH AGAINST CCL4 - INDUCED LIVER DAMAGE
Full text link  Objective: To evaluate the hepatoprotective activity of aqueous extract of Oxalis debilis Kunth in carbon tetrachloride (CCl4)-induced hepatotoxicity in Swiss albino mice.Methods: Hepatotoxicity was induced by CCl4 30% in olive oil (1 ml/kg intraperitoneally). Mice were treated with aqueous extract of O. debilis at doses of 250 and 500 mg/kg body weight orally for 14 days. There were two groups, pre-treatment (once daily for 14 days before CCl4 intoxication) and post-treatment (2, 6, 24, and 48 hrs after CCl4 intoxication). The observed effects were compared with a known hepatoprotective agent, silymarin.Results: Pre-treatment and post-treatment groups of aqueous extract of O. debilis significantly reduced elevated serum levels of serum transaminases, alkaline phosphatase, and bilirubin and increased the level of total protein as compared to CCl4-treated group. The histopathological study also confirms the hepatoprotection. Preliminary qualitative phytochemical analysis of the plant revealed the presence of phenolic compounds, tannins, flavonoids, and saponins.Conclusion: The results of this study suggest that O. debilis can be used as safe, cheap, and alternative preventive and protective drugs against liver injury. The protective effect observed could be attributed to the presence of various phytochemicals which are responsible for the restoration of liver damage
Hydro-physico-chemical Grouping of Cachar Paper Mill Effluents in Assam Using Multivariate Statistical Model
No full textCOMPUTATIONAL STUDY ON THE ANGIOTENSIN CONVERTING ENZYME INHIBITORY POTENTIAL OF THE TEA POLYPHENOLS – CATECHINS: RELEVANCE TO CARDIOVASCULAR DISEASES
Full text linkObjectives: Secondary metabolites obtained from different plants have been the starting material for designing different drugs. Polyphenols are the secondary metabolites that shows myriad varieties of pharmacological activities. Different polyphenols have been found to be cardioprotective and paved the path towards development of cardioprotective formulations. In the present study, we have analyzed the inhibitory potential of tea catechins, a group of polyphenols, on the Angiotensin Converting Enzyme (ACE) - the enzyme responsible for various cardiovascular diseases.Methods: Binding affinity of catechins against known cardioprotective drug target were calculated by performing the docking experiment using FlexX and IC50 values of the compounds were predicted by QSAR analysis.Results: ADMET screening revealed that catechins were non-toxic and obeyed Lipinski\u27s rule. The docking studies showed greater affinity of the catechins towards the active site of drug target. QSAR analysis revealed that the catechins have significant IC 50 values.Conclusion: The study suggests that catechins may be Angiotensin Converting Enzyme targeted potent new drug for treating Cardiovascular diseases.Â
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