1,999,039 research outputs found
An Evaluation of the Deployment of Moderation-Enhanced MOX Fuel Fully Loaded in Pressurized Water Reactor
No full text
CHO microRNA engineering is growing up : recent successes and future challenges
No full textmicroRNAs with their ability to regulate complex pathways that control cellular behavior and phenotype have been proposed as potential targets for cell engineering in the context of optimization of biopharmaceutical production cell lines, specifically of Chinese Hamster Ovary cells. However, until recently, research was limited by a lack of genomic sequence information on this industrially important cell line. With the publication of the genomic sequence and other relevant data sets for CHO cells since 2011, the doors have been opened for an improved understanding of CHO cell physiology and for the development of the necessary tools for novel engineering strategies. In the present review we discuss both knowledge on the regulatory mechanisms of microRNAs obtained from other biological models and proof of concepts already performed on CHO cells, thus providing an outlook of potential applications of microRNA engineering in production cell lines
CHO-K1 B2M assay performance on CHO-K1 genomic DNA.
No full textThe CHO-K1 B2M qPCR assay was tested against a fourfold serial dilution of CHO-K1 genomic DNA spanning 0.025–102.4 ng gDNA. Graphing the Ct versus the log of plasmid copies demonstrates a linear range of 0.025–102.4 ng gDNA with R2 values approaching 1 and slope values close to -3.3.</p
Erratum: Wang, S.Q., Yan, H.F., Cheng, Z.J. & Wang, Y.B. (2023) Primula xingshanensis (Primulaceae), a new species from Hubei, China. Phytotaxa 594 (2): 158-162.
No full textWang, S.Q., Yan, H.F., Cheng, Z.J., Wang, Y.B. (2023): Erratum: Wang, S.Q., Yan, H.F., Cheng, Z.J. & Wang, Y.B. (2023) Primula xingshanensis (Primulaceae), a new species from Hubei, China. Phytotaxa 594 (2): 158-162. Phytotaxa 597 (4): 299-300, DOI: 10.11646/phytotaxa.597.4.6, URL: http://dx.doi.org/10.11646/phytotaxa.597.4.
CHO-S master cell line generation.
No full text(A) The H11 locus was cleaved, using CRISPR/Cas9, to encourage integration of the landing pad donor. A successful knock-in at the H11 locus generates a master cell line that has two PhiC31 attP sites, and that co-expresses three proteins via a PGK promoter-driven transcript. (B) Genotyping of the 5’-arm and 3’-arm in the CHO-S master cell line. PCR was performed with genomic DNA and specific primer pairs. 1: CHO-S parental cell line with 5’-arm primers; 2: CHO-S master cell line ("4–6") with 5’-arm primers; 3: CHO-S parental cell line with 3’-arm primers; 4: CHO-S master cell line ("4–6") with 3’-arm primers.</p
- …
