170,887 research outputs found
CHO microRNA engineering is growing up : recent successes and future challenges
No full textmicroRNAs with their ability to regulate complex pathways that control cellular behavior and phenotype have been proposed as potential targets for cell engineering in the context of optimization of biopharmaceutical production cell lines, specifically of Chinese Hamster Ovary cells. However, until recently, research was limited by a lack of genomic sequence information on this industrially important cell line. With the publication of the genomic sequence and other relevant data sets for CHO cells since 2011, the doors have been opened for an improved understanding of CHO cell physiology and for the development of the necessary tools for novel engineering strategies. In the present review we discuss both knowledge on the regulatory mechanisms of microRNAs obtained from other biological models and proof of concepts already performed on CHO cells, thus providing an outlook of potential applications of microRNA engineering in production cell lines
sj-docx-2-cho-10.1177_18632521241229618 – Supplemental material for MR-based Bony 3D models enable radiation-free preoperative patient-specific analysis and 3D printing for SCFE patients
No full textSupplemental material, sj-docx-2-cho-10.1177_18632521241229618 for MR-based Bony 3D models enable radiation-free preoperative patient-specific analysis and 3D printing for SCFE patients by Till D Lerch, Tilman Kaim, Valentin Grob, Markus Hanke, Florian Schmaranzer, Simon D Steppacher, Jasmin D Busch and Kai Ziebarth in Journal of Children’s Orthopaedics</p
Fujio Cho Legacy Lecture Notes
No full textThe Fujio Cho legacy lecture was created in 2013 as part of UK Institute of Research for Technology Development (IR4TD)’s True Lean Systems program to assist industrial clients to transform their organizations to a True Lean organization by effectively using principles and tools of Toyota Production System (TPS). During the years 1987-1994, Cho and Saito worked together to layout the foundation of the now well-established Toyota-University of Kentucky relationship on R&D, True Lean Systems, and production engineering, housed in IR4TD, the Toyota endowed Institute established in 2007 with the support from the Commonwealth of Kentucky under the research competitiveness trust fund. In 2019, this collaboration celebrated its 25th anniversary by recognizing True Lean Systems program serving over 30,000 people in eighteen different countries worldwide and 48 states nationally.
The idea of Fujio Cho Legacy Lecture Notes (FCLLN) was suggested to honor his wisdom and vision which are vital to maintain IR4TD/True Lean Systems program. FCLLN is written to provide philosophical and cultural background of TPS and Goroku, which are mentioned in the Fujio Cho legacy lecture. However, the human side of TPS, Hitozukuri, the manufacturing side of TPS, Monozukuri, and their interaction are not easily explained during an hour-long Cho lecture. Therefore, FCLLN plays into that role for attendees of IR4TD/True Lean Systems’ certification, and general audiences who are interested in TPS and are familiar with the concepts of Hitozukuri and Japanese Monozukuri culture. FCLLN covers a total of ten chapters: Chapter I. Introduction Chapter II. Toyota Production System and Goroku Chapter III. TPS and Wisdom Chapter IV. TPS and Empathetic Listening Chapter V. TPS as Unique Product of Japanese Culture Chapter VI. Deductive Science and Inductive TPS Chapter VII. Top-Down Power-Driven System vs. Bottom-up Kaizen System Chapter VIII. Cho Goroku on Service Chapter IX. Eastern Philosophy, Mother Teresa, and TPS Chapter X. Finally, the West and the East came together under the same principlehttps://uknowledge.uky.edu/ir4td_textbooks/1000/thumbnail.jp
1ST MEASUREMENT OF GAMMA(D(S)(+)-]MU+NU)/GAMMA(D(S)(+)-]PHI-PI+)
No full textComplete Author List:
ACOSTA D, ATHANAS M, MASEK G, PAAR H, BEAN A, GRONBERG J, KUTSCHKE R, MENARY S, MORRISON RJ, NAKANISHI S, NELSON HN, NELSON TK, RICHMAN JD, RYD A, TAJIMA H, SCHMIDT D, SPERKA D, WITHERELL MS, PROCARIO M, YANG S, BALEST R, CHO K, DAOUDI M, FORD WT, JOHNSON DR, LINGEL K, LOHNER M, RANKIN P, SMITH JG, ALEXANDER JP, BEBEK C, BERKELMAN K, BESSON D, BROWDER TE, CASSEL DG, CHO HA, COFFMAN DM, DRELL PS, EHRLICH R, GALIK RS, GARCIASCIVERES M, GEISER B, GITTELMAN B, GRAY SW, HARTILL DL, HELTSLEY BK, JONES CD, JONES SL, KANDASWAMY J, KATAYAMA N, KIM PC, KREINICK DL, LUDWIG GS, MASUI J, MEVISSEN J, MISTRY NB, NG CR, NORDBERG E, OGG M, PATTERSON JR, PETERSON D, RILEY D, SALMAN S, SAPPER M, WORDEN H, WURTHWEIN F, AVERY P, FREYBERGER A, RODRIGUEZ J, STEPHENS R, YELTON J, CINABRO D, HENDERSON S, KINOSHITA K, LIU T, SAULNIER M, SHEN F, WILSON R, YAMAMOTO H, ONG B, SELEN M, SADOFF AJ, AMMAR R, BALL S, BARINGER P, COPPAGE D, COPTY N, DAVIS R, HANCOCK N, KELLY M, KWAK N, LAM H, KUBOTA Y, LATTERY M, NELSON JK, PATTON S, PERTICONE D, POLING R, SAVINOV V, SCHRENK S, WANG R, ALAM MS, KIM IJ, NEMATI B, ONEILL JJ, SEVERINI H, SUN CR, ZOELLER MM, CRAWFORD G, DAUBENMIER CM, FULTON R, FUJINO D, GAN KK, HONSCHEID K, KAGAN H, KASS R, LEE J, MALCHOW R, MORROW F, SKOVPEN Y, SUNG M, WHITE C, WHITMORE J, WILSON P, BUTLER F, FU X, KALBFLEISCH G, LAMBRECHT M, ROSS WR, SKUBIC P, SNOW J, WANG PL, WOOD M, BORTOLETTO D, BROWN DN, FAST J, MCILWAIN RL, MIAO T, MILLER DH, MODESITT M, SCHAFFNER SF, SHIBATA EI, SHIPSEY IPJ, WANG PN, BATTLE M, ERNST J, KROHA H, ROBERTS S, SPARKS K, THORNDIKE EH, WANG CH, DOMINICK J, SANGHERA S, SHELKOV V, SKWARNICKI T, STROYNOWSKI R, VOLOBOUEV I, ZADOROZHNY P, ARTUSO M, HE D, GOLDBERG M, HORWITZ N, KENNETT R, MONETI GC, MUHEIM F, MUKHIN Y, PLAYFER S, ROZEN Y, STONE S, THULASIDAS M, VASSEUR G, ZHU G, BARTELT J, CSORNA SE, EGYED Z, JAIN V, SHELDON P, AKERIB DS, BARISH B, CHADHA M, CHAN S, COWEN DF, EIGEN G, MILLER JS, OGRADY C, URHEIM J, WEINSTEIN A
The antihypertensive effects of the Jamaican Cho-Cho (Sechium edule)
No full textThe experiments reported in this study constitute a preliminary investigation into the possible hypotensive effect of the Jamaican Cho-Cho (Sechium edule). Experiments were conducted in a random and blind fashion on two sub species of Sechium edule. Both the pulp and the peel were examined for hypotensive activity. Water-soluble extracts were prepared from these components of the fruit and injected into anaesthetised rats. Various cardiovascular parameters were measured including heart rate, mean arterial pressure (MAP) and several ECG intervals. We report that all extracts tested produced a fall in blood pressure with little change in ECG intervals. Extract B produced the least change in heart rate with a fall in MAP of approximately 23 mmHg. Changes in heart rate with all extracts appeared to be minimal as an ED25 value could only be determined for extract A, and ED10 values could not be evaluated for extracts C and D. The mechanism(s) by which these extracts produce their hypotensive effects could not be determined in these preliminary experiments. However, it appears not to involve direct effects on cardiac tissue. This conclusion is based on the finding that it took a minimum of 10 to 15 seconds for the hypotensive action to manifest post bolus. Future experiments will be aimed at delineating the mechanism(s) involved in decreasing MAP.Peer reviewedfinal article publishe
CHO-K1, COS7, and HEK293 cells express PAR1 and PAR2 receptors.
No full textA. CHO-K1, COS7, and HEK293 cells naturally express high levels of PAR1 and PAR2 mRNA but express little or no PAR3 and PAR4 mRNA. qPCR analysis was used to quantify the mRNA expression. Specific primers for each of PAR1, PAR2, PAR3, and PAR4, were used to quantify the respective mRNA expression using cDNA made from each cell line as the templates. β-actin primers were used to quantify β-actin mRNA expression as the internal controls. The relative mRNA expression of PAR1, PAR2, PAR3, and PAR4 were first normalized using β-actin expression, and then normalized using the PAR1 expression level in CHO-K1 cells, which is arbitrarily set as 100%. The relative expressions of other genes are represented as percentage of PAR1 mRNA level in CHO-K1 cells. The results shown are mean ± sd (n = 3). Statistical analysis (One-Way ANOVA) shows that compared with the mRNA expression of PAR4, which is undetectable in these cells, CHO cells express high levels of mRNAs for PAR1 (** p = 0.0037), PAR2 (* p = 0.023), and PAR3 (* p = 0.035); COS7 and HEK293 cells express high level of mRNAs for PAR1 (** p = 0.0029, * p = 0.032, respectively) and PAR2 (** p = 0.0013, ** p = 0.0027, respectively) without expressing detectable PAR3 and PAR4 mRNAs. B, C, D. CHO-K1, COS7, and HEK293 cells naturally express PAR1 and PAR2 receptors and respond to thrombin (PAR1 ligand) and trypsin (PAR2 ligand) stimulations. FLIPR assays were used measure receptor activation as indicated by intracellular Ca2+ mobilization. Relative fluorescent units (RFU) are the readout for fluorescent intensities for Ca2+ mobilization signals. Various concentration of thrombin or trypsin were used as the ligands to activation the receptors. The assays were performed in triplicates at each data point and mean ± sd are shown. E. Sequencing analysis of the genomic DNA from par1 & par2 knock out HEK293 cells. The results show that a 270 bp deletion in par1 gene and a 347 bp deletion in par2 gene have been achieved. The deletions removed the coding regions from TM2 to TM3 for both PAR1 and PAR2 proteins. The vertical lines indicate the deletion sites. F. Characterization of par1 & par2 knock-out HEK293 cells. FLIPR assays were used to characterize receptor activation as indicated. Wild type HEK293 cells were used as the positive control. The assays were performed in triplicates at each data point and mean ± sd are shown.</p
GRP78 protects CHO cells from ribosylation
No full text
D-Ribose (Rib), a reactive glycation compound that exists in organisms, abnormally increases in the urine of diabetic patients and can yield large amounts of advanced glycation end products (AGEs), leading to cell dysfunction. However, whether cellular proteins are sensitive to this type of glycation is unknown. In this study, we found that cellular AGEs accumulate in Chinese hamster ovary (CHO) cells with increased Rib concentration and administration time. Mass spectrum analysis of isolated AGE-modified proteins from cell lysates showed that glucose-regulated protein 78 kD (GRP78) is one of the main ribosylated proteins. Co-immunoprecipitation assays further confirmed the interaction between AGEs and GRP78. Compared with D-glucose (Glc), Rib produced much more AGEs in cells. In kinetic studies, the first order rate constant of LDH released from CHO cells incubated with Rib was nearly 8-fold higher than that of Glc, suggesting that Rib is highly cytotoxic. Immunofluorescent co-localization analysis manifested partial superimposition of AGEs and GRP78, which were distributed throughout the endoplasmic reticulum. Western blotting showed that the expression of GRP78 is up-regulated and then down-regulated in CHO cells during Rib treatment. In the presence of Rib, the suppression of GRP78 expression either with transfected siRNA or with the inhibitor (-)-epigallocatechin gallate (EGCG) dramatically increased AGE levels and decreased cell viability compared with these parameters in the control groups. GRP78 over-expression decreased AGE levels and rescued the cells from Rib-induced cytotoxicity. These data indicate that GRP78 plays a role in preventing Rib-induced CHO cell cytotoxicity.</p
sj-docx-1-cho-10.1177_18632521231192462 – Supplemental material for Hip Impingement of severe SCFE patients after in situ pinning causes decreased flexion and forced external rotation in flexion on 3D-CT
No full textSupplemental material, sj-docx-1-cho-10.1177_18632521231192462 for Hip Impingement of severe SCFE patients after in situ pinning causes decreased flexion and forced external rotation in flexion on 3D-CT by Till D Lerch, Young-Jo Kim, Ata Kiapour, Adam Boschung, Simon D Steppacher, Moritz Tannast, Klaus A Siebenrock and Eduardo N Novais in Journal of Children’s Orthopaedics</p
MeSH term explosion and author rank improve expert recommendations
No full textInformation overload is an often-cited phenomenon that reduces the productivity, efficiency and efficacy of scientists. One challenge for scientists is to find appropriate collaborators in their research. The literature describes various solutions to the problem of expertise location, but most current approaches do not appear to be very suitable for expert recommendations in biomedical research. In this study, we present the development and initial evaluation of a vector space model-based algorithm to calculate researcher similarity using four inputs: 1) MeSH terms of publications; 2) MeSH terms and author rank; 3) exploded MeSH terms; and 4) exploded MeSH terms and author rank. We developed and evaluated the algorithm using a data set of 17,525 authors and their 22,542 papers. On average, our algorithms correctly predicted 2.5 of the top 5/10 coauthors of individual scientists. Exploded MeSH and author rank outperformed all other algorithms in accuracy, followed closely by MeSH and author rank. Our results show that the accuracy of MeSH term-based matching can be enhanced with other metadata such as author rank
1H NMR Spectroscopy Profiling of Metabolic Reprogramming of Chinese Hamster Ovary Cells upon a Temperature Shift during Culture
No full textWe report an NMR based approach to determine the metabolic reprogramming of Chinese hamster ovary cells upon
a temperature shift during culture by investigating the extracellular cell culture media and intracellular metabolome of
CHOK1 and CHO-S cells during culture and in response to cold-shock and subsequent recovery from hypothermic
culturing. A total of 24 components were identified for CHOK1 and 29 components identified for CHO-S cell systems
including the observation that CHO-S media contains 5.6 times the level of glucose of CHOK1 media at time zero.
We confirm that an NMR metabolic approach provides quantitative analysis of components such as glucose and
alanine with both cell lines responding in a similar manner and comparable to previously reported data. However,
analysis of lactate confirms a differentiation between CHOK1 and CHO-S and that reprogramming of metabolism in
response to temperature was cell line specific. The significance of our results is presented using principal component
analysis (PCA) that confirms changes in metabolite profile in response to temperature and recovery. Ultimately, our
approach demonstrates the capability of NMR providing real-time analysis to detect reprogramming of metabolism
upon cellular perception of cold-shock/sub-physiological temperatures. This has the potential to allow manipulation of
metabolites in culture supernatant to improve growth or productivity
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