54,564 research outputs found
CHO microRNA engineering is growing up : recent successes and future challenges
Full text linkmicroRNAs with their ability to regulate complex pathways that control cellular behavior and phenotype have been proposed as potential targets for cell engineering in the context of optimization of biopharmaceutical production cell lines, specifically of Chinese Hamster Ovary cells. However, until recently, research was limited by a lack of genomic sequence information on this industrially important cell line. With the publication of the genomic sequence and other relevant data sets for CHO cells since 2011, the doors have been opened for an improved understanding of CHO cell physiology and for the development of the necessary tools for novel engineering strategies. In the present review we discuss both knowledge on the regulatory mechanisms of microRNAs obtained from other biological models and proof of concepts already performed on CHO cells, thus providing an outlook of potential applications of microRNA engineering in production cell lines
1H NMR Spectroscopy Profiling of Metabolic Reprogramming of Chinese Hamster Ovary Cells upon a Temperature Shift during Culture
Full text linkWe report an NMR based approach to determine the metabolic reprogramming of Chinese hamster ovary cells upon
a temperature shift during culture by investigating the extracellular cell culture media and intracellular metabolome of
CHOK1 and CHO-S cells during culture and in response to cold-shock and subsequent recovery from hypothermic
culturing. A total of 24 components were identified for CHOK1 and 29 components identified for CHO-S cell systems
including the observation that CHO-S media contains 5.6 times the level of glucose of CHOK1 media at time zero.
We confirm that an NMR metabolic approach provides quantitative analysis of components such as glucose and
alanine with both cell lines responding in a similar manner and comparable to previously reported data. However,
analysis of lactate confirms a differentiation between CHOK1 and CHO-S and that reprogramming of metabolism in
response to temperature was cell line specific. The significance of our results is presented using principal component
analysis (PCA) that confirms changes in metabolite profile in response to temperature and recovery. Ultimately, our
approach demonstrates the capability of NMR providing real-time analysis to detect reprogramming of metabolism
upon cellular perception of cold-shock/sub-physiological temperatures. This has the potential to allow manipulation of
metabolites in culture supernatant to improve growth or productivity
Thermal neutron cross-section and resonance integral of the W-186(n, gamma)W-187 reaction
No full textWe measured the thermal neutron cross-section and the resonance integral of the reaction W-186(n, gamma)W-187 by the activation method using a Au-197(n, gamma)Au-198 monitor reaction as single comparator. The high-purity natural W and Au metallic foils with and without a cadmium shield case of 0.5 mm thickness were irradiated in a neutron field of the Pohang neutron facility. The induced activities in the samples were measured by high-resolution gamma-ray spectrometry with a calibrated p-type high-purity Ge detector. The necessary correction factors for gamma-ray attenuation (F-g), thermal neutron self-shielding (G(th)), and resonance neutron self-shielding (G(epi)) effects, and the epithermal neutron spectrum shape factor (alpha) were taken into account. The thermal neutron cross-section for the W-186(n, gamma)W-187 reaction has been determined to be 37.2 +/- 2.1 barn, relative to the reference value of 98.65 +/- 0.09 barn for the Au-197(n, gamma)Au-198 reaction. The present result is, in general, in good agreement with most of the experimental data and the recently evaluated value of ENDF/BVII.0 by 5.7%. By assuming the cadmium cut-off energy of 0.55 eV, the resonance integral obtained is 461 +/- 39 barn, which is determined relative to the reference values of 1550 +/- 28 barn for the Au-197(n, gamma)Au-198 reaction. The present resonance integral value is in general good agreement with the recently measured values by 9%. The present result is lower than the evaluated ones by 10-13%. (C) 2008 Elsevier B.V. All rights reserved.11sciescopu
Polarity effect of pulsed corona discharge for the oxidation of gaseous elemental mercury
No full textThe effect of polarity on the oxidation of Hg(0) was examined in the presence of O(2) via a pulsed corona discharge (PCD). The experimental result showed no difference in the energy yield of Hg(0) oxidation at both positive and negative PCDs (similar to 8 mu g Hg W h(-1) at following conditions: total flow rate = 2 L min(-1) (Hg(0) = 50 mu g N m(-3), O(2) = 10%, and N(2) balance), temperature = 150 degrees C, and specific energy density = 5-15 W h N m(-3)). This suggests that the positive PCD process used to control gaseous air pollutants may play an essential key role in Hg(0) oxidation because it consumes enough energy (similar to 15 W h N m(-3)) but an electrical precipitator could not because it consumes less energy (similar to 0.3 W h N m(-3)) to oxidize Hg(0). (C) 2011 Elsevier Ltd. All rights reserved.X1178sciescopu
Correspondence and Photograph from Ernest W. Wright to Dr. C. A. Bacote, August 30, 1977
No full textCorrespondence between Ernest W. Wright (Employment Security Agency, Georgia Department of Labor) and Dr. C. A. Bacote, 30 August 1977. Enclosed 8"x10" photograph circa 1955, depicting voter registration participants. Written on Verso: "Left to Right: (Seated): Dr. Clarence A. Bacote, Dr. William Hale, Mr. Ron Harris? Dr. Robert Brisbane (Standing): Mr. Michael? Mr. C. A. Scott? Mr. Milton White"
Editorial: Molecular Genetics of Cutaneous Melanoma: Current Status and Future Direction
Full text linkFaust : Romance Of The Secret Tribunals / By George W. M. Reynolds, Author Of "The Mysteries Of London", "Pickwick Abroad", &c. Illustrated In The First Style Of The Art
No full textFAUST : ROMANCE OF THE SECRET TRIBUNALS / BY GEORGE W. M. REYNOLDS, AUTHOR OF "THE MYSTERIES OF LONDON", "PICKWICK ABROAD", &C. ILLUSTRATED IN THE FIRST STYLE OF THE ART
Faust : Romance Of The Secret Tribunals / By George W. M. Reynolds, Author Of "The Mysteries Of London", "Pickwick Abroad", &c. Illustrated In The First Style Of The Art (1)
Cover (1)
Umschlagtitel (8)
Titelseite (9)
Contents (10)
Illustrations / Prologue (11)
Prologue (12)
Chapter I.-XCV. (14)
Epilogue (119
Letter from C. W. Pence, Colonel, 442nd Infantry Regiment, Commanding, Headquarters, 442nd Combat Team, to Kiku Saito, May 20, 1943
No full textA letter from C. W. Pence, Colonel, 442nd Infantry Regiment to Kiku Saito incarcerated in the Granada camp, Colorado. It informs her of her son, Minoru Frank Saito's arrival at Camp Shelby, Mississippi. It congratulates her for his enlistment and quoted the President Roosevelt's statement, "Americanism is not, and never was, a matter of race or ancestry. A good American is one who is loyal to this country and to our creed of liberty and democracy.
Correspondence, C. W. Tayleure to John Brown, Jr., June 18, 1879
No full textA letter to C. W. Tayleure to John Brown, Jr. concerning the death of Brown's brother, Watson. 5 pages
Label-free protein quantification of sodium butyrate treated CHO cells by ESI-UHR-TOF-MS
No full textMüller B, Heinrich C, Jabs W, et al. Label-free protein quantification of sodium butyrate treated CHO cells by ESI-UHR-TOF-MS. Journal of Biotechnology. 2017;257:87-98
- …
