10,933 research outputs found
Interview of author Walter Satterthwait
No full textWalter Satterthwait, author of a series of contemporary crime novels, talks about his protagonists Joshua Croft and Rita Mondragon, and his novels set in Santa Fe, New Mexico. Satterthwait describes how he came to writing crime stories and why he chose to use a Latina as a main character. He describes his exposure to different cultures, his childhood of frequent moves, how he came to writing, and how he developed his characters. Satterthwait is interviewed by Diana Rivera at the 2005 Left Coast Crime Conference held in El Paso, Texas
HARNESSING GENOME EDITING TO ELUCIDATE THE ROLE OF HISTONE VARIANT H2A.Z IN GRAPEVINE
No full textPlants continually encounter various environmental stresses that represent significant challenges to their growth, development, and survival. In
particular, grapevine, which is a perennial plant of great economic importance, is subjected to both abiotic stresses, such as drought and
extreme temperatures, and biotic stresses, including diseases like powdery (Erysiphe necator) or downy (Plasmopara viticola) mildew. Lastly, due to a long history of breeding programs, crops have developed the "domestication syndrome". This involves an imbalance in the plant use of resources towards growth and productivity, at the expense of resistance to pathogens and the ability to recruit beneficial microorganisms, such as mycorrhizae.
Epigenetic modifications, such as DNA methylation and histone modifications, play an important role in stress responsiveness. Histone
variants can be incorporated into nucleosomes in a targeted and replication independent manner thus influencing nucleosome structure and properties, gene transcription, DNA repair mechanisms and finally genome stability. Despite this, there is still much to be discovered about histone variants, especially on their role during plant-microorganism interactions. Currently, H2A.Z, one variant of the canonical histone H2A, seems to be the
most involved in these mechanisms. Indeed, in plant, H2A.Z enrichment in the nucleosome occurs at Transcription starting site (TSS) of active genes
and in the gene body of silent inducible genes. Plant mutants of histone H2A.Z variants that have been produced so far are viable, and therefore can
reveal the function of these chromatin regulators throughout the life of the plant. Recent advances in genome editing techniques have provided powerful tools for precise modification of the genome. Our study aim is to investigate the application of genome editing (CRISPR-Cas9) techniques to modulate the expression of H2A.Z genes in grapevine and elucidate their role in in the interaction between plant and microorganisms, both beneficial and pathogenic. Target genes of interest for editing have been identified and transformation efforts are underway, aimed at both downregulation and
overexpression of the H2A.Z genes. This will provide a wider knowledge of the role of H2A.Z in biotic interactions in grapevine
First Report of Alternaria alternata Complex Causing Leaf Spot on Vitis vinifera in Italy
No full text
Anuson Walter Vella
No full textCremation volume for Vella, Walter F. (Walter Francis), 1924-1980, American author on Thailand; comprises condolences and papers on Thailand by both crematee and others
Infrared thermography to estimate vine water status: Optimizing canopy measurements and thermal indices for the varieties Merlot and moscato in northern Italy
No full textThis study investigated the effectiveness of infrared thermography to estimate water status in Merlot and Moscato grown in northeast Italy by comparing the crop water stress index (CWSI) and the stomatal conductance index (IG). The influence of the portion of the canopy in which the thermal images were captured (sunlit or shaded) was also investigated. During the 2018 growing season, potted vines were subjected to three irrigation treatments: T0 = 100% of daily water usage restored; T1 = 50% of daily water usage restored; and T2 = 30% of daily water usage restored. Measurements included stomatal conductance (gs), stem water potential (SWP), and thermal imagery. Results showed that both the CWSI and IG indices were effective in discriminating the irrigation treatments in Moscato and Merlot. CWSI showed higher correlations with gs and SWP compared to IG, especially in Moscato. CWSI was less influenced by the portion of the canopy the image was taken on. In general, Moscato showed greater differences in gs, SWP, and the thermal indices between the three irrigation treatments. This study suggests that the efficacy of thermography in estimating vine water status depends on the variety and its stomatal control physiology.Nicola Belfiore, Rosanna Vinti, Lorenzo Lovat, Walter Chitarra, Diego Tomasi, Roberta de Bei, Franco Meggio, and Federica Gaiott
Letter from Walter M. Weglyn to Frank Chin, May 30, 1989
No full textA letter from Walter M. Weglyn to Frank Chin praising him for his writings about Japanese Americans draft resisters during World War II.These materials are from box 73 and 74 of the Frank Chin Papers. The Frank Chin Papers contain personal and professional correspondence between Frank Chin and Michi Weglyn relating to particular projects on which either author was working as well as files related to the Day of Remembrance Tribute to Michi Weglyn
Letter from Michi and Walter Weglyn to Frank Chin, July 25, 1993
No full textA personal letter from Michi and Walter Weglyn to Frank Chin.These materials are from box 73 and 74 of the Frank Chin Papers. The Frank Chin Papers contain personal and professional correspondence between Frank Chin and Michi Weglyn relating to particular projects on which either author was working as well as files related to the Day of Remembrance Tribute to Michi Weglyn
Microbe-assisted crop improvement: a sustainable weapon to restore holobiont functionality and resilience
No full textIn the past years, breeding programs have been mainly addressed on pushing the commercial features, forgetting important traits, such as those related to environmental stress resilience, that are instead present in wild relatives. Among the traits neglected by breeding processes, the ability to recruit beneficial microorganisms that recently is receiving a growing attention due to its potentiality. In this context, this review will provide a spotlight on critical issues of the anthropocentric point of view that, until now, has characterized the selection of elite plant genotypes. Its effects on the plant-microbiome interactions, and the possibility to develop novel strategies mediated by the exploitation of beneficial root-microbe interactions, will be discussed. More sustainable microbial-assisted strategies might in fact foster the green revolution and the achievement of a more sustainable agriculture in a climatic change scenario
Actinomycetes come to rescue of viticulture sustainability
No full textA great number of recent researches have shown that Actinomycetes can be considered as promising beneficial bacteria improving the growth and the capacity of the plants to face up both biotic and abiotic stresses. However, very few studies have been performed about the use of Actinomycetes in grapevine and all of them have focused their attention just on those isolated from the soil. For this reason, we decided to isolate bacteria from grapevine wood tissues characterizing a collection of 42 isolates, 27 of which belonging to the Actinobacteria phylum. These bacteria have been isolated from twenty years old grapevines without visual symptoms of wood diseases and subsequently used in dual culture assay against the main grape pathogens such as B. cinerea and the etiological agents of esca syndrome. Additionally, we are analysing antifungal effects of diffusible and volatile organic compounds (VOCs) produced by the isolates that previously showed a promising antagonistic activity against fungal pathogens. More studies are now ongoing to better understand their potential ability to promote plant growth features both in vitro and in vivo. Indeed, we are testing these bacteria on in vitro grapevine plants and on rooted cuttings. In the vitro assay we are evaluating their individual capacity to promote plant growth measuring the number of leaves and the roots length. Regarding to the rooted cuttings experiment, we inoculated a microbial consortium consisting of five Actinomycetes and three bacteria belonging to different phyla. The aim of the experiment is to test the effect of these beneficial bacteria both alone and in combination with some mycorrhizae fungi. We are now collecting several physiological parameters of the rooted cuttings to confirm the ability of these bacteria to increase the plant growth and wellness. More studies are needed but preliminary results display an interesting attitude of these actinomycetes as promising tool to improve viticulture sustainability
- …
