1,721,100 research outputs found
The validity of tissue factor assays as diagnostic tool for cancer: correlation between urinary tissue factor and monocyte tissue factor activity
No full textAdvances in urinary tissue factor measurements: clinical application of a new methodology
No full textAssociation between monocyte tissue factor, tumour presence and progression in urological cancer
No full textDevelopment and validation of an assay for urinary tissue factor activity
No full textBACKGROUND: Activation of blood coagulation is a common complication of cancer and inflammation in both humans and experimental animals. Increased production of tissue factor--the principal initiator of the coagulation process--by endothelial cells, monocytes, and macrophages has been implicated in these conditions.AIM: To investigate whether urinary tissue factor (uTF) might reflect the state of monocyte/macrophage activation and be a useful diagnostic test.METHODS: Urine was centrifuged at 51,000 g to sediment tissue factor containing membrane vesicles. The tissue factor was then solubilised in beta-octyl-glucopyranoside and assayed in a specific chromogenic assay adapted for use in microtitre plates.RESULTS: The assay proved to be sensitive, specific, and reproducible. The normal range of uTF was relatively narrow and unaffected by age, sex, or cigarette smoking. Levels were not significantly influenced by storage of urine samples before assay or by the presence of fresh blood in the urine sample.CONCLUSIONS: This method may have diagnostic application in the study of haemostatic activation in patients with cancer and other disease states
Tissue factor assays in cancer: correlation between urinary and monocyte tissue factor activity
No full text
Monocyte tissue factor activity in controls and patients with breast and colorectal cancer
No full textGeorge-G. Chisholm, M, A. B. Sc. Geography and Commerce (La Géographie et le Commerce), septembre 1907
No full textGoegg Egmond. George-G. Chisholm, M, A. B. Sc. Geography and Commerce (La Géographie et le Commerce), septembre 1907. In: Le Globe. Revue genevoise de géographie, tome 47, 1908. pp. 173-177
Urinary tissue factor in urological cancer: associations with tumour presence and progression
No full textMonocyte tissue factor levels in cancer patients
No full textOBJECTIVE: The association between cancer and thromboembolic disease has been known for over a century. Increased tissue factor expression by endothelial cells, monocytes or macrophages is implicated. Thus, monocyte tissue factor measurements may reflect disease presence or progression.METHODS: Using a 2 stage kinetic chromogenic assay, monocyte tissue factor levels were assessed in normal controls (n=60), patient controls (hernia or cholecystectomy, n=60) and in patients with benign and malignant disease of the bladder (n=73), prostate (n=81), breast (n=83) and colorectum (n=62). This was performed as baseline (resting cells) and after 6 hours incubation with (stimulated) and without (unstimulated) lipopolysaccharide. Each benign disease group was sub-divided into inflammatory and non-inflammatory categories.RESULTS: The relative operating characteristic curve for the lipopolysaccharide-stimulated monocyte tissue factor assay showed sensitivity and specificity for cancer, the area under the curve being 0.71. The control groups and the benign non-inflammatory groups gave similar results and were pooled for further analysis. Each malignant group showed higher monocyte tissue factor levels than the control groups for baseline (P< 0.05) and lipopolysaccharide-stimulated cells (P< 0.05). All benign inflammatory groups apart from breast, showed increased monocyte tissue factor levels over controls for baseline (P< 0.05) and lipopolysaccharide-stimulated cells (P< 0.05). In all cases there was no significant difference between the malignant and the benign inflammatory groups. Monocyte tissue factor levels were related to tumor grade or stage, patients' survival time, serum prostate specific antigen and static bone scan images. Levels were also higher in patients with bladder cancer recurrence and in those who subsequently died.CONCLUSION: Lipopolysaccharide-stimulated monocyte tissue factor assay showed sensitivity and specificity for cancer compared to controls. Monocyte tissue factor levels are raised in malignant groups compared to controls and non-inflammatory diseases but not when compared with inflammatory conditions. Stimulated cells give better discrimination between the groups and may be useful in identifying high risk individuals. Monocyte tissue factor levels were related to tumor progression
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