1,720,976 research outputs found
Comparative fingerprinting analysis of Escherichia coli O157:H7 isolates from foods by amplified fragment polymorphism
No full textStaphylococcus aureus enterotossigeno e meticillino-resistente (MRSA) in prodotti lattiero caseari della Puglia (Italia)-Dati preliminari
No full textPestivirus infection in small ruminants: virological and histo-pathological findings
No full textPestivirus infection was detected in several flocks of sheep and goats located in the south of Italy by means of serological, virological and histopathological investigations. From four animals, two lambs and two kids, showing enteric symptoms which died during the first week of life, four pestivirus strains were isolated and typed as BVDV-like (three isolates) and "tipic" BDV strains (one isolate). The histopathological lesions consisted of areas of hypomyelination in the brain, and a slight depletion of thymic medullary lymphocytes associated with an increase in reticular cells
Comparison between three vaccines against canine leihmaniasis: in vitro evaluation of macrophage killing ability
No full textDetection of anthrax vaccine virulence factors by polymerase chain reaction
No full textIn Italy, an attenuated Bacillus anthracis strain, named 'Carbosap', is used for immunization against ovine and bovine anthrax. Analysis on 'Carbosap', Sterne vaccine strain F34 and Pasteur vaccine strain SS104, were performed using primers specific for the sequences, encoding the toxic factors, located on plasmids pXO1 and pXO2 and primers specific for the chromosome. The results obtained from polymerase chain reaction (PCR) assay revealed the presence of both plasmids pXO1 and pXO2 in 'Carbosap' strain. This study showed that the 'Carbosap' vaccine strain has a different plasmid pattern in comparison to Pasteur vaccine strain SS104 and Sterne vaccine strain F34
PCR assay to detect Bacillus anthracis spores in heat-treated specimens
Full text linkRecent interest in anthrax is due to its potential use in bioterrorism and as a biowarfare agent against civilian populations. The development of rapid and sensitive techniques to detect anthrax spores in suspicious specimens is the most important aim for public health. With a view to preventing exposure of laboratory workers to viable Bacillus anthracis spores, this study evaluated the suitability of PCR assays for detecting anthrax spores previously inactivated at 121 degrees C for 45 min. The results indicate that heat treatment ensures the complete inactivation of B. anthracis spores without significantly affecting the efficiency of PCR assays
In vitro evaluation of the Enrofloxacin activity on killing ability of canine macrophages against Leishmania infantum
No full textVibrio parahaemolyticus, v. vulnificus and microrganisms of fecal origin in mussels (Mytilus galloprovincialis) sold in the Puglia Region (Italy)
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