177 research outputs found
An electromagnetically-driven microfluidic platform with indirect-heating thermo-pneumatic valves
No full text
The integration of safety and ergonomics into a lean manufacturing processa case study
No full textIncludes bibliographical references
CD8+ T Lymphocyte Self-Renewal during Effector Cell Determination
No full textSummarySelected CD8+ T cells must divide, produce differentiated effector cells, and self-renew, often repeatedly. We now show that silencing expression of the transcription factor TCF1 marks loss of self-renewal by determined effector cells and that this requires cell division. In acute infections, the first three CD8+ T cell divisions produce daughter cells with unequal proliferative signaling but uniform maintenance of TCF1 expression. The more quiescent initial daughter cells resemble canonical central memory cells. The more proliferative, effector-prone cells from initial divisions can subsequently undergo division-dependent production of a TCF1-negative effector daughter cell along with a self-renewing TCF1-positive daughter cell, the latter also contributing to the memory cell pool upon resolution of infection. Self-renewal in the face of effector cell determination may promote clonal amplification and memory cell formation in acute infections, sustain effector regeneration during persistent subclinical infections, and be rate limiting, but remediable, in chronic active infections and cancer
Reading silence actively: Recovering the maternal narrative in contemporary women's novels
No full textThe author has granted permission for their work to be available to the general public.This project uses an interdisciplinary methodology derived from linguistic, rhetorical, critical race, feminist, and third-space feminist theories to examine how close discursive analysis reveals counter-hegemonic tendencies in maternal characters who use silence as a source of linguistic empowerment. In my analysis, I compare novels published post-1985 by both white and black American women to demonstrate an emerging cross-racial dialectic concerning American feminist mothering and the role of silence in literature. Throughout my dissertation, I explore how silence has been used by contemporary women authors publishing post-1985 to subvert various forms of oppression, as well as to recover via a palimpsestic methodology matrilineal heritages that have been left unwritten. Specifically, I focus on Sherley Anne Williams's Dessa Rose (1986), Ellen Douglas's Can't Quit You, Baby (1988), Kaye Gibbons's Ellen Foster (1987), Dori Sanders's Clover (1990), Sapphire's PUSH (1996), Kim Edwards's The Memory Keeper's Daughter (2005), Alice Randall's The Wind Done Gone (2001), and Nancy Rawles's My Jim (2005). Throughout this project, I demonstrate the progressive, transformational use of silence as a rhetorical strategy by contemporary American women writers as a discursive method of non-oppositional feminist dialogue.Englis
The Plural of Us
No full textThis is the first book to focus on the poet's use of the first-person plural voice—poetry's “we.” Closely exploring the work of W. H. Auden, the book uncovers the trove of thought and feeling carried in this small word. While lyric has long been associated with inwardness and a voice saying “I,” “we” has hardly been noticed, even though it has appeared throughout the history of poetry. Reading for this pronoun in its variety and ambiguity, the book's author explores the communal function of poetry—the reasons, risks, and rewards of the first-person plural. The author adopts a taxonomic approach to her subject, considering “we” from its most constricted to its fully unbounded forms. The author also takes a historical perspective, following Auden's interest in the full range of “the human pluralities” in a time of particular pressure for and against the collective. Examples from many other poets—including Walt Whitman, T. S. Eliot, Elizabeth Bishop, and Wallace Stevens—arise throughout the book, and the final chapter offers a consideration of how contemporary writers find form for what George Oppen called “the meaning of being numerous.” Connecting insights to philosophy of language and to recent work in concepts of community, the book shows how poetry raises vital questions—literary and social—about how we speak of our togetherness.</p
The Canadian Elder Standard - Pricing the Cost of Basic Needs for the Canadian Elderly
No full textWe determined the after-tax income required to fi nance basic needs for Canadian elders living with different circumstances in terms of age, gender, city of residence, household size, homeowner or renter status, means of transportation, and health status. Using 2001 as our base year, we priced the typical expenses for food, shelter, medical, transportation, miscellaneous basic living items and home-based long-term care for elders living in fi ve Canadian cities. This is the fi rst Canadian study of basic living expenses tailored to elders instead of adults in general, prepared on an absolute rather than a relative basis. We also accounted for an individual’s unique life circumstances and established the varying effect that they have on the cost of basic expenses, particularly for home care. We found that the maximum Guaranteed Income Supple ment and Old Age Security benefi t did not meet the cost of basic needs for an elder living in poor circumstances.Canadian seniors, poverty measure, economic security, aging-in-place, cost-of-living, absolute measure, home care
Development of a mouse model system for studying the regulated expression of CD40L
No full textCD4+ T cells are part of the adaptive immune response and are critical for providing signals for orchestrating the immune response. Through the expression of the CD40L, CD4+ T cells interact and activate macrophages, B-cells, dendritic cells, and CD8+ T cells. Previous data showed that during early times of activation, the CD40L mRNA is highly transcribed and rapidly degraded, while at late times transcription is decreased and the mRNA is stabilized by a polypyrimidine tracks binding complex in the 3’ UTR region.To broaden our understanding of the CD40L stability pathway and its impact on overall CD4+ adaptive immune response, we characterized the mouse CD40L 3’ UTR region, assembled a targeting construct with a deletion in the CD40L 3’ UTR stability element, and through homologous recombination generated a mouse model. Characterization of the CD40L 3’ UTR region was performed using both luciferase and DRB RNA decay assays. We concluded that region mA led to the instability of the 3’ UTR, while regions mB and mC contributed to its stability at late times post-activation. A targeting construct was designed to engineer a mouse lacking the CD40L stability region in order to determine the effect on the strength of the humoral response in these CD40LΔ5 mice. Analysis of the mutant mice revealed differences in the availability of CD40L on the surface of CD4+ cells (40% decrease in the CD40LΔ5 mice), organization of germinal centers (with CD40LΔ5 mice having more disorganized GC), and differences in IgM and IgG levels during a secondary immune response.M.S.Includes bibliographical reference
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