31,505 research outputs found
A common pathway for detoxification of 4-hydroxybenzylglucosinolate in Pieris and Anthocaris (Lepidoptera: Pieridae).
No full textAgerbirk N, Olsen CE, Chew F, Müller C. A common pathway for detoxification of 4-hydroxybenzylglucosinolate in Pieris and Anthocaris (Lepidoptera: Pieridae). Biochemical Systematics and Ecology. 2006;34(3):189-198
Getting Started as a Medical Teacher in Times of Change
No full textMedical school teaching is a skill that is very often learned on the job. The faculty comprised of researchers and clinicians are expert in many biomedical disciplines, but familiarity with learning theories and pedagogy are usually not included in their knowledge and skill sets. The pressure to see patients and acquire extramural funding leaves little time for faculty to learn how to teach. When coupled with the natural attrition of senior faculty it is necessary to start junior faculty on the correct path to being effective medical educators who are capable of lecturing and facilitating. Institutions cannot afford to have medical educators learn through trial and error. The standards set by the Liaison Committee on Medical Education (LCME) are also creating an urgency to produce competent teachers as quickly as possible. Novice teachers need to be able to use these standards to align their teaching with goals, objectives and the appropriate pedagogy. This article is designed to be a self-directed guide describing some essentials that a newly hired faculty member can quickly use to get started. An institutional faculty development program can then serve to build upon and enrich the experience for the new faculty member.This is the authors' accepted manuscript of the article. The final publication is available at Springer via http://dx.doi.org/doi:10.1007/s40670-014-0098-y.Peer reviewe
Ryhiner-Kartensammlung / 8/B Carte de la baye de Chin-Chew ou Chang-Chew avec les isles d'Emowi et de Quemowi
No full textdressée sur divers manuscrits, par N. B[ellin] ing.r de la marineTitel unten links, Massstabsleiste unten rechtsOrtsnamen "Chin-Chew" und "Chang-Chew" mit Angabe von Schreibvarianten (oberhalb "ew" jeweils "ou")Numerierung unten rechts: "Tom. V. No. 22."Ursprungswerk: Band 5 der "Histoire générale des voyages" von A. F. Prévost (Paris : Didot, 1748
Sous-facteurs de L(F∞) d'indice 4cos2π/n,n≥3
No full textLet Q be a factor of type II1, λ a number in the Jones discrete series {4cosπ/m:m≥3}, and {ei} the Jones projections associated with λ. Denote by A2n and A1n the finite-dimensional von Neumann algebras generated, respectively, by {1,e2,⋯,en} and {1,e1,⋯,en}, with the corresponding traces. The author shows that, for n sufficiently large, the index of the inclusion An=(Q⊗A2n)∗A2nA1n⊂(Q⊗A2n+1)∗A2n+1A1n+1=An+1 is equal to λ (here ∗ denotes the reduced, amalgamated free product of the algebras in question). Using the random matrix model of Voiculescu, he proves that if Q is the von Neumann algebra L(F∞) of the free group with infinitely many generators, then An is isomorphic to L(F∞).
The two facts together imply the existence, for any λ in the Jones discrete series, of an irreducible subfactor of L(F∞) of index λ. This constitutes the first example of a nonhyperfinite, non-Γ II1 factor such that its Jones invariant is fully computable (the existence of nonirreducible subfactors of L(F∞) for any index ≥4 is a simple consequence of known results)
A proteomic investigation of Fusobacterium nucleatum alkaline-induced biofilms
Get PDFBackground: The Gram negative anaerobe Fusobacterium nucleatum has been implicated in the aetiology of periodontal diseases. Although frequently isolated from healthy dental plaque, its numbers and proportion increase in plaque associated with disease. One of the significant physico-chemical changes in the diseased gingival sulcus is increased environmental pH. When grown under controlled conditions in our laboratory, F. nucleatum subspecies polymorphum formed mono-culture biofilms when cultured at pH 8.2. Biofilm formation is a survival strategy for bacteria, often associated with altered physiology and increased virulence. A proteomic approach was used to understand the phenotypic changes in F. nucleatum cells associated with alkaline induced biofilms. The proteomic based identification of significantly altered proteins was verified where possible using additional methods including quantitative real-time PCR (qRT-PCR), enzyme assay, acidic end-product analysis, intracellular polyglucose assay and Western blotting. Results: Of 421 proteins detected on two-dimensional electrophoresis gels, spot densities of 54 proteins varied significantly (p < 0.05) in F. nucleatum cultured at pH 8.2 compared to growth at pH 7.4. Proteins that were differentially produced in biofilm cells were associated with the functional classes; metabolic enzymes, transport, stress response and hypothetical proteins. Our results suggest that biofilm cells were more metabolically efficient than planktonic cells as changes to amino acid and glucose metabolism generated additional energy needed for survival in a sub-optimal environment. The intracellular concentration of stress response proteins including heat shock protein GroEL and recombinational protein RecA increased markedly in the alkaline environment. A significant finding was the increased abundance of an adhesin, Fusobacterial outer membrane protein A (FomA). This surface protein is known for its capacity to bind to a vast number of bacterial species and human epithelial cells and its increased abundance was associated with biofilm formation. Conclusion: This investigation identified a number of proteins that were significantly altered by F. nucleatum in response to alkaline conditions similar to those reported in diseased periodontal pockets. The results provide insight into the adaptive mechanisms used by F. nucleatum biofilms in response to pH increase in the host environment.Jactty Chew, Peter S Zilm, Janet M Fuss and Neville J Gull
Postać n-tej iteracji operatora q = f d/dx
No full textArtykuł nie zawiera streszczeniaMotivated by applications in linear dynamical systems, the author studies q^n(f), where q is the operator f●(d/dx) and qn is its n-th iteration. q^n(f) is a polynomial F(f(0),f(1),...,f(n)) in the derivatives f(0)=f,...,f(n) of f with integer coefficients. Special attention is paid to determining the coefficients of F. The author presents algorithms for computing the coefficients and also shows that the sum of all coefficients of F equals n!. The paper ends with some remarks on the number of coefficients of F, which is related to the number-theoretic unrestricted partition function
Effects of the mutation R62A on the global and local backbone dynamics of CheW.
No full text<p>Backbone amide <sup>15</sup>N relaxation parameters for CheW vs. residue number are shown. The black squares represent wild-type CheW, the red circles represent R62A mutant, and the green triangles represent the difference between these two constructs. Approximate location of secondary structural elements is shown at the top: (a) the longitudinal relaxation rate R<sub>1</sub>; (b) the transverse relaxation rate R<sub>2</sub>; (c) <sup>1</sup>H-<sup>15</sup>N NOE; (d) the extracted order parameter S<sup>2</sup> (e) the differences phenomenological transverse relaxation rate constant ΔR<sub>ex</sub> = R<sub>ex</sub>(20 ms)−R<sub>ex</sub>(1 ms); (f) the differences between the ΔR<sub>ex</sub> in (e) ΔR<sub>ex</sub>(R62A)−ΔR<sub>ex</sub>(WT).</p
Ratio of n-6/n-3 in the diets of beef cattle
No full textEffects of feeding heat-treated canola (C), soybean (S) and flax (F) or mixtures on growth and slaughter characteristics, taste and fatty acid (FA) composition of beef tissue were investigated using 128 crossbred steers to determine the potential of improving the nutritional quality of beef for humans. For Trial 1 (48 steers), dietary treatments were: roasted C, extruded C, roasted S, extruded S, roasted F and extruded F. For Trial 2 (80 steers), the dietary treatments were: S:F (1:1), S:C (1:1), C:F (1:1) and S:F:C (1:1:1), and the oilseeds were processed either by roasting or extruding before mixing. Soybean meal and soybean oil were used to give equivalent lipid and protein contents to each experimental diet. The basal diet consisted of grass silage, barley grain, vitamins and minerals. Steers were fed for a minimum of 100d then slaughtered at a uniform degree of finish. Growth and slaughter characteristics of the steers were only slightly affected by dietary treatment in that the soybean-fed steers consumed more feed and had a higher average daily gain than the canola or flax-fed animals in Trial 1. There was no difference in taste panel parameters for any of the treatments. Inclusion of flax in the diet increased the total n-3 content of meat. Similar results were found for canola and C18:1n-9 although this was not the case for soybean and the n-6 FA. For the n-6 FA in the PL and neutral lipid fractions of the meat samples, levels were correlated with high dietary levels of n-6 or n-9 with low levels of n-3 while for the n-3 FA, levels were correlated with high dietary n-3 levels and low n-6 levels. Oilseed processing method did not have an effect on any fatty acid levels. It is possible to modify the FA composition of beef meat toward a healthier profile by including heat-treated oilseeds in the diet to influence the degree of lipid metabolism in the rumen.ID: S0377840111004007; M3: Article; Accession Number: S0377840111004007; Author: M.A. McNiven (a, ⁎); Author: J.L. Duynisveld (b); Author: T. Turner (a); Author: A.W. Mitchell (a); Affiliation: Department of Health Management, Atlantic Veterinary College, University of PEI, Charlottetown, PEI, Canada C1A 4P3; Affiliation: Agriculture and Agri-Food Canada, Nappan, NS, Canada B0L 1C0; Keyword: Oilseeds; Keyword: Roasted; Keyword: Extruded; Keyword: Fatty acids; Keyword: Healthy fat; Number of Pages: 11; Language: English
In vitro reconstitution of the TlpD, CheW, and CheA chemotaxis signaling complex.
No full text(A) Kinetics of HpCheA with varying concentrations of ATP are shown. Experiments were conducted in triplicate with 4 μM CheA and varying amounts of ATP in 50 mM Tris (pH 7.5), 100 mM NaCl, and 10 mM MgCl2. The average kobs for three replicate time courses are shown at various concentrations of ATP (black dots), error bars are the sample standard deviation, and these measurements are fit to the Michaelis-Menten curve (black line). (B) Representative analytical ultracentrifugation data (black axes) are shown for TlpD at 1 μM (black dotted black line) in PBS buffer (pH 7) with 1 mM TCEP. At this concentration, peaks corresponding to the TlpD monomer and dimer occur near 3.5 [S] and 5.2 [S], respectively. The average dimer KD for the recombinant TlpD construct calculated across various protein concentrations was found to be 188 nM (S1 Table). Shown in red on secondary axes are fluorescence anisotropy data for a titration of TlpD under identical conditions with experiments run in triplicate. See also S1 Fig. for a comparison of the TlpD dimer KD with the N-terminal His tag present or cleaved off and simulated data showing the expected monomer and dimer populations expected based on measured KD values. (C) Shown on top are representative raw data from radio-ATP labeling experiments of 15-minute reactions with CheA alone (“A”) and additions of CheW (“AW”) and TlpD (“AWD”). Below are reactions of 1 mM ATP and 4 μM CheA (black circles), +8 μM CheW (blue triangles), and +8 μM CheW; 24 μM TlpD (red squares) run in triplicate and fit to a pseudo–first-order reaction curve (solid lines). (D) CheW was titrated against 4 μM CheA and resulting kobs measurements were fit to a binding isotherm to estimate a kinetically-defined KD of 14.6 μM for the CheA↔CheW interaction. (E) TlpD was titrated against 4 μM CheA and 40 μM CheW and fit to a binding isotherm as in panel C to approximate the thermodynamics of the CheA, CheW↔TlpD interaction to have a KD of 15.2 μM. (F) A titration of TlpD against 4 μM CheA shows no activation (gray squares). For CheA in the presence of saturating [CheW], a 2.7-fold activation occurs (blue line), and with saturating [CheW] and [TlpD], this is increased to a 14.6-fold activation (red line) over CheA alone (black line). See S1 Table for a summary of reaction parameters and statistics. CheA, chemotaxis protein A; CheA-Pi, phosphorylated CheA; CheW, chemotaxis protein W; HpCheA, Helicobacter pylori CheA; S, Sedverg; TCEP, tris(2-carboxyethyl)phosphine; TlpD, transducer-like protein D.</p
Fast implementation of iterative adaptive approach for wideband unambiguous radar detection
No full textAccepted author manuscriptMicrowave Sensing, Signals & System
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