96 research outputs found
A TALEN-mediated gene correction for Wiskott-Aldrich Syndrome using a single- stranded Oligodeoxynucleotide donor template
No full textWiskott-Aldrich syndrome (WAS) is an X-linked primary immunodeficiency
disorder caused by mutations in the WAS gene, resulting in defects in the gene product, the WAS protein (WASP). WASP directs the formation of polymerized actin filaments during the cytoskeletal rearrangements required for immune system surveillance and homeostasis. Currently, the only cure for WAS is a bone marrow transplant. This study aimed to correct a known gene mutation causing WAS in human induced pluripotent stem cells (iPSCs). A pair of TALENs were constructed to target a known gene mutation in WAS iPSCs. A single strand oligodeoxynucleotide served as a donor template for the wild-type WAS gene, leading to a gene correction via homology-directed recombination
(HDR). The efficiency of HDR was approximately 7% in 293T cells, but was 0% in WAS iPSCs. This approach may represent a useful strategy for autologous gene therapy in WAS and other inherited human diseases
A TALEN-mediated gene correction for Wiskott-Aldrich Syndrome using a single- stranded Oligodeoxynucleotide donor template
No full textWiskott-Aldrich syndrome (WAS) is an X-linked primary immunodeficiency disorder caused by mutations in the WAS gene, resulting in defects in the gene product, the WAS protein (WASP). WASP directs the formation of polymerized actin filaments during the cytoskeletal rearrangements required for immune system surveillance and homeostasis. Currently, the only cure for WAS is a bone marrow transplant. This study aimed to correct a known gene mutation causing WAS in human induced pluripotent stem cells (iPSCs). A pair of TALENs were constructed to target a known gene mutation in WAS iPSCs. A single strand oligodeoxynucleotide served as a donor template for the wild-type WAS gene, leading to a gene correction via homology-directed recombination (HDR). The efficiency of HDR was approximately 7% in 293T cells, but was 0% in WAS iPSCs. This approach may represent a useful strategy for autologous gene therapy in WAS and other inherited human diseases
Counting the Patsy Edwards Benefit Show proceeds
No full textThis 1951 photograph shows three bank executives counting the proceeds donated from the Patsy Edwards Benefit Show. Patsy Edwards was the ballad champion in 1949 and 1951 in the Mountain Youth Jamboree. Her father was accidentally killed while Ms. Edwards was performing for the Dance Masters of America in Washington, D.C. with other Mountain Youth Jamboree contestants. The Jamboree champions gave a benenfit program to assist her and her family. Founder and director of the Mountain Youth Jamboree, Hubert H. Hayes (1901-1964) auditioned and directed youth to perform in folk dance, music, and folk and ballad singing. The jamboree was held in the Asheville City Auditorium (now known as Thomas Wolfe Auditorium) from 1948 to 1973, and Hayes’ wife, Leona Trantham Hayes (1913-1989) continued to direct the program after his death in 1964. Hubert Hayes was an author, playwright, and alumni of Duke University
Patsy Edwards singing with Jimmy Haynie on guitar
No full textThis undated photograph shows Patsy Edwards of Woodfin Jr. High School singing with Jimmy Haynie on guitar. Ms. Edwards was the ballad champion in 1949 and 1951 in the Mountain Youth Jamboree. Founder and director of the Mountain Youth Jamboree, Hubert H. Hayes (1901-1964) auditioned and directed youth to perform in folk dance, music, and folk and ballad singing. The jamboree was held in the Asheville City Auditorium (now known as Thomas Wolfe Auditorium) from 1948 to 1973, and Hayes’ wife, Leona Trantham Hayes (1913-1989) continued to direct the program after his death in 1964. Hubert Hayes was an author, playwright, and alumni of Duke University
My Nursery Tale Book
No full textThis is my earliest version of this book. I have two other copies printed in 1970 and 1972. All three of these stem from the 1961 copyright. Earlier copyrights were in 1949 and 1954. This copy differs from the 1970 printing by using the blue page facing the title-page as a paste-down inside front cover. The same is true in the back of the book. Thus two nice illustrations are not included, as they are on the first page facing the blank inside cover and the corresponding last page facing the blank inside back cover: Pierre Bear reading a book (excerpted from the front cover's picture and the first picture in "Pierre Bear") and Duck sleeping in bed. As I wrote on the 1970 version, TMCM told by Patsy Scarry has a quaint flapper city mouse in its water colors. The picture of the cat discovering the two mice may be the best. This story adds a vacuum cleaner! The picture on the back cover has a dog never mentioned in the story! FC by the same author adds an elephant and a tiger to its cast of characters. Overall, I continue to believe that there is little special here for the lover of new and different understandings of fables. Inscribed in 1966.This is a hardbound book (hard cover)1964 printingPatsy Scarry et a
Patsy Nailed the Horse-Shoe
No full textA man hangs a horse shoe upside down and has bad luck.https://egrove.olemiss.edu/kgbsides_uk/2510/thumbnail.jp
Author In Town
No full textPhotograph used for a story in the Daily Oklahoman newspaper. Caption: "Dr. Joyce Brothers, left, visits with Sheron Hendryx and Patsy Sine at a cocktail party preceding a recent dinner given by Maguire Brothers Inc. in the Marriott Hotel's grand ballroom.
A study of thirty-six non-follow-up Negro mothers in Postpartum Clinic, Grady Hospital, Atlanta, Georgia, 1947
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