852 research outputs found
Design and performance of a low-flow capillary electrophoresis-electrospray-mass spectrometry interface using an emitter with dual beveled edge
No full textA beveled tip sheath liquid interface for capillary electrophoresis-electrospray ionization-mass spectrometry
No full textAnalysis of coptisine, berberine and palmatine in adulterated Chinese medicine by capillary electrophoresis–electrospray ion trap mass spectrometry
No full textAnalysis of triazines by reversed electroosmotic flow capillary electrophoresis–electrospray mass spectrometry
No full textA Low-Makeup Beveled Tip Capillary Electrophoresis /Electrospray Ionization Mass Spectrometry Interface for Micellar Electrokinetic Chromatography and Nonvolatile Buffer Capillary Electrophoresis
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Sheathless Capillary Electrophoresis/Electrospray Mass Spectrometry Using a Carbon-Coated Fused-Silica Capillary
No full textA Low-Flow CE/Electrospray Ionization MS Interface for Capillary Zone Electrophoresis, Large-Volume Sample Stacking, and Micellar Electrokinetic Chromatography
No full textQuantitative proteomic analysis of metabolic regulation by copper ions in Methylococcus capsulatus (Bath)
No full text[[abstract]]Copper ions switch the oxidation of methane by soluble methane monooxygenase to particulate methane monooxygenase in Methylococcus capsulatus ( Bath). Toward understanding the change in cellular metabolism related to this transcriptional and metabolic switch, we have undertaken genomic sequencing and quantitative comparative analysis of the proteome in M. capsulatus ( Bath) grown under different copper-to-biomass ratios by cleavable isotope-coded affinity tag technology. Of the 682 proteins identified, the expressions of 60 proteins were stimulated by at least 2-fold by copper ions; 68 proteins were down-regulated by 2-fold or more. The 60 proteins overexpressed included the methane and carbohydrate metabolic enzymes, while the 68 proteins suppressed were mainly responsible for cellular signaling processes, indicating a role of copper ions in the expression of the genes associated with the metabolism of the organism downstream of methane oxidation. The study has also provided a complet
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